This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CATTAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 75.24 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 103,141 (3.6%) Reads that were too short: 9,559 (0.3%) Reads written (passing filters): 93,582 (3.3%) Total basepairs processed: 853,219,115 bp Total written (filtered): 18,969,494 bp (2.2%) === Adapter 1 === Sequence: CATTAG; Type: regular 5'; Length: 6; Trimmed: 103141 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 440 692.0 0 440 7 13276 692.0 0 13276 8 12597 692.0 0 12597 9 91 692.0 0 91 10 56 692.0 0 56 11 44 692.0 0 44 12 37 692.0 0 37 13 36 692.0 0 36 14 54 692.0 0 54 15 38 692.0 0 38 16 38 692.0 0 38 17 84 692.0 0 84 18 55 692.0 0 55 19 39 692.0 0 39 20 73 692.0 0 73 21 48 692.0 0 48 22 53 692.0 0 53 23 49 692.0 0 49 24 44 692.0 0 44 25 43 692.0 0 43 26 57 692.0 0 57 27 79 692.0 0 79 28 69 692.0 0 69 29 124 692.0 0 124 30 46 692.0 0 46 31 51 692.0 0 51 32 50 692.0 0 50 33 58 692.0 0 58 34 60 692.0 0 60 35 74 692.0 0 74 36 52 692.0 0 52 37 79 692.0 0 79 38 57 692.0 0 57 39 72 692.0 0 72 40 42 692.0 0 42 41 54 692.0 0 54 42 59 692.0 0 59 43 49 692.0 0 49 44 55 692.0 0 55 45 74 692.0 0 74 46 45 692.0 0 45 47 45 692.0 0 45 48 48 692.0 0 48 49 40 692.0 0 40 50 83 692.0 0 83 51 70 692.0 0 70 52 91 692.0 0 91 53 61 692.0 0 61 54 78 692.0 0 78 55 53 692.0 0 53 56 132 692.0 0 132 57 103 692.0 0 103 58 523 692.0 0 523 59 245 692.0 0 245 60 352 692.0 0 352 61 52 692.0 0 52 62 64 692.0 0 64 63 37 692.0 0 37 64 42 692.0 0 42 65 49 692.0 0 49 66 49 692.0 0 49 67 50 692.0 0 50 68 72 692.0 0 72 69 79 692.0 0 79 70 119 692.0 0 119 71 129 692.0 0 129 72 1230 692.0 0 1230 73 313 692.0 0 313 74 761 692.0 0 761 75 44 692.0 0 44 76 49 692.0 0 49 77 66 692.0 0 66 78 72 692.0 0 72 79 50 692.0 0 50 80 51 692.0 0 51 81 98 692.0 0 98 82 51 692.0 0 51 83 56 692.0 0 56 84 57 692.0 0 57 85 61 692.0 0 61 86 60 692.0 0 60 87 55 692.0 0 55 88 133 692.0 0 133 89 81 692.0 0 81 90 153 692.0 0 153 91 125 692.0 0 125 92 167 692.0 0 167 93 97 692.0 0 97 94 61 692.0 0 61 95 93 692.0 0 93 96 79 692.0 0 79 97 68 692.0 0 68 98 53 692.0 0 53 99 70 692.0 0 70 100 58 692.0 0 58 101 79 692.0 0 79 102 56 692.0 0 56 103 54 692.0 0 54 104 71 692.0 0 71 105 70 692.0 0 70 106 64 692.0 0 64 107 73 692.0 0 73 108 70 692.0 0 70 109 77 692.0 0 77 110 73 692.0 0 73 111 70 692.0 0 70 112 228 692.0 0 228 113 171 692.0 0 171 114 385 692.0 0 385 115 56 692.0 0 56 116 48 692.0 0 48 117 88 692.0 0 88 118 65 692.0 0 65 119 177 692.0 0 177 120 97 692.0 0 97 121 116 692.0 0 116 122 66 692.0 0 66 123 83 692.0 0 83 124 55 692.0 0 55 125 87 692.0 0 87 126 82 692.0 0 82 127 111 692.0 0 111 128 134 692.0 0 134 129 277 692.0 0 277 130 545 692.0 0 545 131 217 692.0 0 217 132 132 692.0 0 132 133 325 692.0 0 325 134 547 692.0 0 547 135 177 692.0 0 177 136 241 692.0 0 241 137 298 692.0 0 298 138 172 692.0 0 172 139 195 692.0 0 195 140 333 692.0 0 333 141 444 692.0 0 444 142 531 692.0 0 531 143 6243 692.0 0 6243 144 5726 692.0 0 5726 145 31716 692.0 0 31716 146 202 692.0 0 202 147 65 692.0 0 65 148 105 692.0 0 105 149 108 692.0 0 108 150 120 692.0 0 120 151 55 692.0 0 55 152 73 692.0 0 73 153 94 692.0 0 94 154 80 692.0 0 80 155 201 692.0 0 201 156 464 692.0 0 464 157 888 692.0 0 888 158 71 692.0 0 71 159 87 692.0 0 87 160 50 692.0 0 50 161 79 692.0 0 79 162 91 692.0 0 91 163 439 692.0 0 439 164 1050 692.0 0 1050 165 551 692.0 0 551 166 54 692.0 0 54 167 89 692.0 0 89 168 68 692.0 0 68 169 55 692.0 0 55 170 72 692.0 0 72 171 65 692.0 0 65 172 46 692.0 0 46 173 62 692.0 0 62 174 58 692.0 0 58 175 74 692.0 0 74 176 70 692.0 0 70 177 61 692.0 0 61 178 42 692.0 0 42 179 65 692.0 0 65 180 68 692.0 0 68 181 57 692.0 0 57 182 49 692.0 0 49 183 63 692.0 0 63 184 55 692.0 0 55 185 67 692.0 0 67 186 49 692.0 0 49 187 61 692.0 0 61 188 66 692.0 0 66 189 61 692.0 0 61 190 91 692.0 0 91 191 54 692.0 0 54 192 61 692.0 0 61 193 81 692.0 0 81 194 70 692.0 0 70 195 73 692.0 0 73 196 79 692.0 0 79 197 60 692.0 0 60 198 56 692.0 0 56 199 45 692.0 0 45 200 84 692.0 0 84 201 74 692.0 0 74 202 67 692.0 0 67 203 115 692.0 0 115 204 72 692.0 0 72 205 65 692.0 0 65 206 82 692.0 0 82 207 66 692.0 0 66 208 63 692.0 0 63 209 95 692.0 0 95 210 138 692.0 0 138 211 71 692.0 0 71 212 74 692.0 0 74 213 111 692.0 0 111 214 220 692.0 0 220 215 796 692.0 0 796 216 300 692.0 0 300 217 392 692.0 0 392 218 68 692.0 0 68 219 49 692.0 0 49 220 81 692.0 0 81 221 92 692.0 0 92 222 68 692.0 0 68 223 121 692.0 0 121 224 76 692.0 0 76 225 87 692.0 0 87 226 70 692.0 0 70 227 68 692.0 0 68 228 70 692.0 0 70 229 52 692.0 0 52 230 63 692.0 0 63 231 86 692.0 0 86 232 76 692.0 0 76 233 149 692.0 0 149 234 75 692.0 0 75 235 97 692.0 0 97 236 92 692.0 0 92 237 68 692.0 0 68 238 48 692.0 0 48 239 82 692.0 0 82 240 49 692.0 0 49 241 65 692.0 0 65 242 136 692.0 0 136 243 47 692.0 0 47 244 78 692.0 0 78 245 74 692.0 0 74 246 83 692.0 0 83 247 53 692.0 0 53 248 74 692.0 0 74 249 62 692.0 0 62 250 72 692.0 0 72 251 65 692.0 0 65 252 64 692.0 0 64 253 93 692.0 0 93 254 77 692.0 0 77 255 83 692.0 0 83 256 60 692.0 0 60 257 76 692.0 0 76 258 82 692.0 0 82 259 77 692.0 0 77 260 99 692.0 0 99 261 104 692.0 0 104 262 48 692.0 0 48 263 87 692.0 0 87 264 88 692.0 0 88 265 96 692.0 0 96 266 67 692.0 0 67 267 74 692.0 0 74 268 59 692.0 0 59 269 70 692.0 0 70 270 83 692.0 0 83 271 79 692.0 0 79 272 81 692.0 0 81 273 68 692.0 0 68 274 59 692.0 0 59 275 75 692.0 0 75 276 74 692.0 0 74 277 64 692.0 0 64 278 87 692.0 0 87 279 83 692.0 0 83 280 72 692.0 0 72 281 87 692.0 0 87 282 139 692.0 0 139 283 83 692.0 0 83 284 87 692.0 0 87 285 111 692.0 0 111 286 101 692.0 0 101 287 76 692.0 0 76 288 79 692.0 0 79 289 79 692.0 0 79 290 56 692.0 0 56 291 82 692.0 0 82 292 85 692.0 0 85 293 76 692.0 0 76 294 92 692.0 0 92 295 102 692.0 0 102 296 172 692.0 0 172 297 159 692.0 0 159 298 118 692.0 0 118 299 88 692.0 0 88 300 63 692.0 0 63 301 72 692.0 0 72 Finished in 3.66 s (39 us/read; 1.53 M reads/minute). === Summary === Total reads processed: 93,582 Reads with adapters: 12,672 (13.5%) Reads that were too short: 48 (0.1%) Reads written (passing filters): 12,624 (13.5%) Total basepairs processed: 18,969,494 bp Total written (filtered): 3,485,575 bp (18.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 12672 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 7 0.0 3 0 0 7 16 82 0.0 3 0 80 2 17 12420 0.0 3 12200 215 4 1 18 28 0.0 3 1 26 1 19 5 0.0 3 1 0 3 1 20 14 0.0 3 0 0 0 14 21 1 0.0 3 0 0 0 1 28 10 0.0 3 0 0 0 10 31 2 0.0 3 0 0 0 2 34 1 0.0 3 1 36 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 48 24 0.0 3 0 0 0 24 50 1 0.0 3 0 0 0 1 51 5 0.0 3 0 0 0 5 59 3 0.0 3 0 0 0 3 61 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 74 2 0.0 3 0 0 0 2 79 2 0.0 3 0 0 0 2 81 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 90 2 0.0 3 0 0 0 2 96 1 0.0 3 0 0 0 1 100 2 0.0 3 0 0 0 2 101 1 0.0 3 0 0 0 1 106 6 0.0 3 0 0 0 6 108 1 0.0 3 0 0 0 1 135 19 0.0 3 0 0 0 19 138 1 0.0 3 0 0 1 140 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 150 12 0.0 3 1 0 0 11 158 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 230 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.44 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 12,624 Reads with adapters: 102 (0.8%) Reads written (passing filters): 12,624 (100.0%) Total basepairs processed: 3,485,575 bp Total written (filtered): 3,485,267 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 102 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 9.8% C: 63.7% G: 23.5% T: 2.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 100 197.2 0 100 4 2 49.3 0 2