This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCACT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 75.12 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 222,937 (7.9%) Reads that were too short: 10,892 (0.4%) Reads written (passing filters): 212,045 (7.5%) Total basepairs processed: 853,219,115 bp Total written (filtered): 39,522,035 bp (4.6%) === Adapter 1 === Sequence: AGCACT; Type: regular 5'; Length: 6; Trimmed: 222937 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 75 692.0 0 75 7 4380 692.0 0 4380 8 4728 692.0 0 4728 9 16 692.0 0 16 10 16 692.0 0 16 11 18 692.0 0 18 12 76 692.0 0 76 13 40 692.0 0 40 14 73 692.0 0 73 15 40 692.0 0 40 16 14 692.0 0 14 17 23 692.0 0 23 18 17 692.0 0 17 19 21 692.0 0 21 20 27 692.0 0 27 21 30 692.0 0 30 22 19 692.0 0 19 23 19 692.0 0 19 24 16 692.0 0 16 25 71 692.0 0 71 26 61 692.0 0 61 27 69 692.0 0 69 28 30 692.0 0 30 29 31 692.0 0 31 30 97 692.0 0 97 31 26 692.0 0 26 32 32 692.0 0 32 33 27 692.0 0 27 34 41 692.0 0 41 35 48 692.0 0 48 36 45 692.0 0 45 37 79 692.0 0 79 38 31 692.0 0 31 39 36 692.0 0 36 40 35 692.0 0 35 41 26 692.0 0 26 42 29 692.0 0 29 43 50 692.0 0 50 44 45 692.0 0 45 45 26 692.0 0 26 46 27 692.0 0 27 47 30 692.0 0 30 48 46 692.0 0 46 49 49 692.0 0 49 50 24 692.0 0 24 51 85 692.0 0 85 52 28 692.0 0 28 53 37 692.0 0 37 54 48 692.0 0 48 55 44 692.0 0 44 56 38 692.0 0 38 57 44 692.0 0 44 58 40 692.0 0 40 59 37 692.0 0 37 60 53 692.0 0 53 61 50 692.0 0 50 62 65 692.0 0 65 63 77 692.0 0 77 64 37 692.0 0 37 65 136 692.0 0 136 66 77 692.0 0 77 67 107 692.0 0 107 68 44 692.0 0 44 69 40 692.0 0 40 70 36 692.0 0 36 71 57 692.0 0 57 72 65 692.0 0 65 73 36 692.0 0 36 74 46 692.0 0 46 75 25 692.0 0 25 76 37 692.0 0 37 77 43 692.0 0 43 78 108 692.0 0 108 79 80 692.0 0 80 80 212 692.0 0 212 81 103 692.0 0 103 82 90 692.0 0 90 83 56 692.0 0 56 84 82 692.0 0 82 85 51 692.0 0 51 86 76 692.0 0 76 87 134 692.0 0 134 88 204 692.0 0 204 89 323 692.0 0 323 90 808 692.0 0 808 91 1474 692.0 0 1474 92 362 692.0 0 362 93 368 692.0 0 368 94 814 692.0 0 814 95 312 692.0 0 312 96 273 692.0 0 273 97 521 692.0 0 521 98 653 692.0 0 653 99 464 692.0 0 464 100 523 692.0 0 523 101 834 692.0 0 834 102 1114 692.0 0 1114 103 1598 692.0 0 1598 104 31120 692.0 0 31120 105 30902 692.0 0 30902 106 65777 692.0 0 65777 107 284 692.0 0 284 108 46 692.0 0 46 109 39 692.0 0 39 110 85 692.0 0 85 111 54 692.0 0 54 112 92 692.0 0 92 113 65 692.0 0 65 114 86 692.0 0 86 115 503 692.0 0 503 116 91 692.0 0 91 117 1157 692.0 0 1157 118 57 692.0 0 57 119 77 692.0 0 77 120 35 692.0 0 35 121 86 692.0 0 86 122 70 692.0 0 70 123 79 692.0 0 79 124 1091 692.0 0 1091 125 59 692.0 0 59 126 140 692.0 0 140 127 48 692.0 0 48 128 56 692.0 0 56 129 67 692.0 0 67 130 55 692.0 0 55 131 36 692.0 0 36 132 163 692.0 0 163 133 38 692.0 0 38 134 45 692.0 0 45 135 31 692.0 0 31 136 37 692.0 0 37 137 36 692.0 0 36 138 96 692.0 0 96 139 66 692.0 0 66 140 61 692.0 0 61 141 88 692.0 0 88 142 168 692.0 0 168 143 69 692.0 0 69 144 113 692.0 0 113 145 71 692.0 0 71 146 78 692.0 0 78 147 117 692.0 0 117 148 69 692.0 0 69 149 64 692.0 0 64 150 61 692.0 0 61 151 124 692.0 0 124 152 127 692.0 0 127 153 181 692.0 0 181 154 188 692.0 0 188 155 230 692.0 0 230 156 344 692.0 0 344 157 734 692.0 0 734 158 1234 692.0 0 1234 159 24629 692.0 0 24629 160 12032 692.0 0 12032 161 10528 692.0 0 10528 162 71 692.0 0 71 163 43 692.0 0 43 164 40 692.0 0 40 165 40 692.0 0 40 166 58 692.0 0 58 167 322 692.0 0 322 168 124 692.0 0 124 169 185 692.0 0 185 170 17 692.0 0 17 171 62 692.0 0 62 172 102 692.0 0 102 173 131 692.0 0 131 174 79 692.0 0 79 175 60 692.0 0 60 176 45 692.0 0 45 177 48 692.0 0 48 178 48 692.0 0 48 179 55 692.0 0 55 180 67 692.0 0 67 181 45 692.0 0 45 182 166 692.0 0 166 183 41 692.0 0 41 184 139 692.0 0 139 185 121 692.0 0 121 186 84 692.0 0 84 187 74 692.0 0 74 188 87 692.0 0 87 189 119 692.0 0 119 190 44 692.0 0 44 191 54 692.0 0 54 192 35 692.0 0 35 193 37 692.0 0 37 194 36 692.0 0 36 195 34 692.0 0 34 196 77 692.0 0 77 197 48 692.0 0 48 198 178 692.0 0 178 199 100 692.0 0 100 200 762 692.0 0 762 201 260 692.0 0 260 202 203 692.0 0 203 203 35 692.0 0 35 204 42 692.0 0 42 205 61 692.0 0 61 206 57 692.0 0 57 207 61 692.0 0 61 208 49 692.0 0 49 209 50 692.0 0 50 210 32 692.0 0 32 211 41 692.0 0 41 212 38 692.0 0 38 213 71 692.0 0 71 214 166 692.0 0 166 215 35 692.0 0 35 216 47 692.0 0 47 217 40 692.0 0 40 218 87 692.0 0 87 219 139 692.0 0 139 220 100 692.0 0 100 221 77 692.0 0 77 222 32 692.0 0 32 223 53 692.0 0 53 224 113 692.0 0 113 225 41 692.0 0 41 226 71 692.0 0 71 227 44 692.0 0 44 228 98 692.0 0 98 229 72 692.0 0 72 230 85 692.0 0 85 231 62 692.0 0 62 232 62 692.0 0 62 233 54 692.0 0 54 234 59 692.0 0 59 235 56 692.0 0 56 236 47 692.0 0 47 237 70 692.0 0 70 238 128 692.0 0 128 239 122 692.0 0 122 240 680 692.0 0 680 241 755 692.0 0 755 242 674 692.0 0 674 243 152 692.0 0 152 244 52 692.0 0 52 245 44 692.0 0 44 246 46 692.0 0 46 247 59 692.0 0 59 248 80 692.0 0 80 249 55 692.0 0 55 250 40 692.0 0 40 251 72 692.0 0 72 252 56 692.0 0 56 253 61 692.0 0 61 254 64 692.0 0 64 255 80 692.0 0 80 256 85 692.0 0 85 257 70 692.0 0 70 258 68 692.0 0 68 259 80 692.0 0 80 260 50 692.0 0 50 261 35 692.0 0 35 262 43 692.0 0 43 263 53 692.0 0 53 264 54 692.0 0 54 265 74 692.0 0 74 266 62 692.0 0 62 267 93 692.0 0 93 268 109 692.0 0 109 269 95 692.0 0 95 270 87 692.0 0 87 271 52 692.0 0 52 272 59 692.0 0 59 273 84 692.0 0 84 274 126 692.0 0 126 275 102 692.0 0 102 276 157 692.0 0 157 277 165 692.0 0 165 278 236 692.0 0 236 279 272 692.0 0 272 280 355 692.0 0 355 281 182 692.0 0 182 282 182 692.0 0 182 283 654 692.0 0 654 284 64 692.0 0 64 285 45 692.0 0 45 286 61 692.0 0 61 287 50 692.0 0 50 288 59 692.0 0 59 289 67 692.0 0 67 290 77 692.0 0 77 291 65 692.0 0 65 292 86 692.0 0 86 293 92 692.0 0 92 294 102 692.0 0 102 295 91 692.0 0 91 296 93 692.0 0 93 297 49 692.0 0 49 298 63 692.0 0 63 299 86 692.0 0 86 300 186 692.0 0 186 301 202 692.0 0 202 Finished in 7.28 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 212,045 Reads with adapters: 4,604 (2.2%) Reads that were too short: 106 (0.0%) Reads written (passing filters): 4,498 (2.1%) Total basepairs processed: 39,522,035 bp Total written (filtered): 1,213,829 bp (3.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 4604 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 1 0.0 3 0 0 1 16 46 0.0 3 0 45 1 17 4231 0.0 3 4152 78 0 1 18 9 0.0 3 0 9 19 10 0.0 3 1 0 8 1 20 7 0.0 3 1 0 0 6 23 15 0.0 3 0 0 0 15 28 4 0.0 3 0 0 0 4 44 10 0.0 3 0 0 0 10 46 16 0.0 3 0 0 0 16 50 1 0.0 3 0 0 0 1 53 4 0.0 3 0 0 0 4 54 2 0.0 3 0 0 0 2 58 25 0.0 3 0 0 0 25 62 1 0.0 3 0 0 0 1 65 3 0.0 3 0 0 0 3 68 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 78 2 0.0 3 0 0 0 2 80 1 0.0 3 0 0 0 1 86 2 0.0 3 0 0 0 2 87 61 0.0 3 0 0 2 59 88 30 0.0 3 0 0 0 30 89 1 0.0 3 0 0 0 1 90 12 0.0 3 0 0 1 11 91 9 0.0 3 0 0 0 9 100 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 113 3 0.0 3 0 0 0 3 114 2 0.0 3 0 0 0 2 122 2 0.0 3 0 0 0 2 126 2 0.0 3 0 0 0 2 127 1 0.0 3 0 0 0 1 128 7 0.0 3 0 0 0 7 129 2 0.0 3 0 0 0 2 130 5 0.0 3 0 0 0 5 131 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 135 3 0.0 3 0 0 0 3 137 1 0.0 3 0 0 0 1 138 3 0.0 3 0 0 0 3 139 2 0.0 3 0 0 0 2 141 4 0.0 3 2 0 0 2 142 12 0.0 3 1 1 0 10 143 1 0.0 3 1 145 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 147 3 0.0 3 0 0 0 3 149 2 0.0 3 0 0 0 2 153 1 0.0 3 1 159 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 166 3 0.0 3 0 0 0 3 179 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 188 3 0.0 3 0 0 0 3 189 19 0.0 3 0 0 3 16 224 1 0.0 3 0 0 0 1 226 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.16 s (36 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 4,498 Reads with adapters: 0 (0.0%) Reads written (passing filters): 4,498 (100.0%) Total basepairs processed: 1,213,829 bp Total written (filtered): 1,213,829 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 0 times.