This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TAATGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 167.27 s (48 us/read; 1.24 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 293,987 (8.5%) Reads that were too short: 51,488 (1.5%) Reads written (passing filters): 242,499 (7.0%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 53,291,862 bp (5.1%) === Adapter 1 === Sequence: TAATGA; Type: regular 5'; Length: 6; Trimmed: 293987 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1475 847.3 0 1475 7 48454 847.3 0 48454 8 37370 847.3 0 37370 9 94 847.3 0 94 10 47 847.3 0 47 11 122 847.3 0 122 12 59 847.3 0 59 13 53 847.3 0 53 14 39 847.3 0 39 15 31 847.3 0 31 16 51 847.3 0 51 17 49 847.3 0 49 18 50 847.3 0 50 19 44 847.3 0 44 20 77 847.3 0 77 21 45 847.3 0 45 22 53 847.3 0 53 23 40 847.3 0 40 24 64 847.3 0 64 25 34 847.3 0 34 26 48 847.3 0 48 27 80 847.3 0 80 28 66 847.3 0 66 29 48 847.3 0 48 30 64 847.3 0 64 31 75 847.3 0 75 32 53 847.3 0 53 33 46 847.3 0 46 34 57 847.3 0 57 35 59 847.3 0 59 36 94 847.3 0 94 37 81 847.3 0 81 38 58 847.3 0 58 39 85 847.3 0 85 40 68 847.3 0 68 41 54 847.3 0 54 42 76 847.3 0 76 43 94 847.3 0 94 44 86 847.3 0 86 45 80 847.3 0 80 46 64 847.3 0 64 47 75 847.3 0 75 48 57 847.3 0 57 49 65 847.3 0 65 50 62 847.3 0 62 51 51 847.3 0 51 52 74 847.3 0 74 53 81 847.3 0 81 54 61 847.3 0 61 55 71 847.3 0 71 56 78 847.3 0 78 57 76 847.3 0 76 58 125 847.3 0 125 59 82 847.3 0 82 60 92 847.3 0 92 61 88 847.3 0 88 62 79 847.3 0 79 63 88 847.3 0 88 64 96 847.3 0 96 65 87 847.3 0 87 66 136 847.3 0 136 67 148 847.3 0 148 68 174 847.3 0 174 69 230 847.3 0 230 70 1652 847.3 0 1652 71 2451 847.3 0 2451 72 3875 847.3 0 3875 73 106 847.3 0 106 74 133 847.3 0 133 75 90 847.3 0 90 76 92 847.3 0 92 77 96 847.3 0 96 78 199 847.3 0 199 79 155 847.3 0 155 80 150 847.3 0 150 81 281 847.3 0 281 82 385 847.3 0 385 83 377 847.3 0 377 84 186 847.3 0 186 85 97 847.3 0 97 86 98 847.3 0 98 87 67 847.3 0 67 88 62 847.3 0 62 89 68 847.3 0 68 90 70 847.3 0 70 91 67 847.3 0 67 92 76 847.3 0 76 93 67 847.3 0 67 94 112 847.3 0 112 95 79 847.3 0 79 96 72 847.3 0 72 97 255 847.3 0 255 98 215 847.3 0 215 99 121 847.3 0 121 100 178 847.3 0 178 101 199 847.3 0 199 102 133 847.3 0 133 103 98 847.3 0 98 104 160 847.3 0 160 105 113 847.3 0 113 106 132 847.3 0 132 107 185 847.3 0 185 108 216 847.3 0 216 109 401 847.3 0 401 110 526 847.3 0 526 111 988 847.3 0 988 112 356 847.3 0 356 113 381 847.3 0 381 114 591 847.3 0 591 115 398 847.3 0 398 116 422 847.3 0 422 117 607 847.3 0 607 118 547 847.3 0 547 119 693 847.3 0 693 120 671 847.3 0 671 121 965 847.3 0 965 122 1279 847.3 0 1279 123 2766 847.3 0 2766 124 51007 847.3 0 51007 125 33833 847.3 0 33833 126 15379 847.3 0 15379 127 319 847.3 0 319 128 163 847.3 0 163 129 377 847.3 0 377 130 275 847.3 0 275 131 147 847.3 0 147 132 104 847.3 0 104 133 110 847.3 0 110 134 144 847.3 0 144 135 137 847.3 0 137 136 215 847.3 0 215 137 171 847.3 0 171 138 206 847.3 0 206 139 343 847.3 0 343 140 464 847.3 0 464 141 259 847.3 0 259 142 184 847.3 0 184 143 217 847.3 0 217 144 197 847.3 0 197 145 185 847.3 0 185 146 209 847.3 0 209 147 294 847.3 0 294 148 450 847.3 0 450 149 7821 847.3 0 7821 150 5086 847.3 0 5086 151 886 847.3 0 886 152 122 847.3 0 122 153 85 847.3 0 85 154 102 847.3 0 102 155 140 847.3 0 140 156 112 847.3 0 112 157 179 847.3 0 179 158 161 847.3 0 161 159 114 847.3 0 114 160 113 847.3 0 113 161 101 847.3 0 101 162 186 847.3 0 186 163 217 847.3 0 217 164 207 847.3 0 207 165 184 847.3 0 184 166 115 847.3 0 115 167 75 847.3 0 75 168 101 847.3 0 101 169 108 847.3 0 108 170 103 847.3 0 103 171 131 847.3 0 131 172 84 847.3 0 84 173 79 847.3 0 79 174 79 847.3 0 79 175 88 847.3 0 88 176 102 847.3 0 102 177 93 847.3 0 93 178 114 847.3 0 114 179 117 847.3 0 117 180 119 847.3 0 119 181 247 847.3 0 247 182 335 847.3 0 335 183 440 847.3 0 440 184 157 847.3 0 157 185 155 847.3 0 155 186 115 847.3 0 115 187 102 847.3 0 102 188 122 847.3 0 122 189 180 847.3 0 180 190 143 847.3 0 143 191 91 847.3 0 91 192 80 847.3 0 80 193 101 847.3 0 101 194 75 847.3 0 75 195 130 847.3 0 130 196 127 847.3 0 127 197 111 847.3 0 111 198 162 847.3 0 162 199 110 847.3 0 110 200 96 847.3 0 96 201 85 847.3 0 85 202 105 847.3 0 105 203 149 847.3 0 149 204 150 847.3 0 150 205 164 847.3 0 164 206 125 847.3 0 125 207 209 847.3 0 209 208 228 847.3 0 228 209 136 847.3 0 136 210 182 847.3 0 182 211 164 847.3 0 164 212 175 847.3 0 175 213 171 847.3 0 171 214 162 847.3 0 162 215 161 847.3 0 161 216 131 847.3 0 131 217 140 847.3 0 140 218 184 847.3 0 184 219 236 847.3 0 236 220 377 847.3 0 377 221 422 847.3 0 422 222 496 847.3 0 496 223 1120 847.3 0 1120 224 1361 847.3 0 1361 225 4563 847.3 0 4563 226 15619 847.3 0 15619 227 16178 847.3 0 16178 228 177 847.3 0 177 229 88 847.3 0 88 230 103 847.3 0 103 231 95 847.3 0 95 232 102 847.3 0 102 233 104 847.3 0 104 234 133 847.3 0 133 235 131 847.3 0 131 236 124 847.3 0 124 237 125 847.3 0 125 238 120 847.3 0 120 239 134 847.3 0 134 240 128 847.3 0 128 241 93 847.3 0 93 242 119 847.3 0 119 243 226 847.3 0 226 244 244 847.3 0 244 245 201 847.3 0 201 246 133 847.3 0 133 247 88 847.3 0 88 248 68 847.3 0 68 249 83 847.3 0 83 250 78 847.3 0 78 251 92 847.3 0 92 252 110 847.3 0 110 253 119 847.3 0 119 254 156 847.3 0 156 255 127 847.3 0 127 256 143 847.3 0 143 257 174 847.3 0 174 258 164 847.3 0 164 259 158 847.3 0 158 260 115 847.3 0 115 261 112 847.3 0 112 262 133 847.3 0 133 263 173 847.3 0 173 264 131 847.3 0 131 265 100 847.3 0 100 266 133 847.3 0 133 267 146 847.3 0 146 268 159 847.3 0 159 269 93 847.3 0 93 270 89 847.3 0 89 271 75 847.3 0 75 272 111 847.3 0 111 273 130 847.3 0 130 274 108 847.3 0 108 275 93 847.3 0 93 276 105 847.3 0 105 277 86 847.3 0 86 278 93 847.3 0 93 279 95 847.3 0 95 280 90 847.3 0 90 281 91 847.3 0 91 282 100 847.3 0 100 283 102 847.3 0 102 284 102 847.3 0 102 285 77 847.3 0 77 286 89 847.3 0 89 287 74 847.3 0 74 288 73 847.3 0 73 289 96 847.3 0 96 290 77 847.3 0 77 291 75 847.3 0 75 292 60 847.3 0 60 293 77 847.3 0 77 294 103 847.3 0 103 295 91 847.3 0 91 296 77 847.3 0 77 297 89 847.3 0 89 298 126 847.3 0 126 299 85 847.3 0 85 300 95 847.3 0 95 301 111 847.3 0 111 Finished in 16.59 s (68 us/read; 0.88 M reads/minute). === Summary === Total reads processed: 242,499 Reads with adapters: 39,061 (16.1%) Reads that were too short: 91 (0.0%) Reads written (passing filters): 38,970 (16.1%) Total basepairs processed: 53,291,862 bp Total written (filtered): 10,577,155 bp (19.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 39061 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 0 2 18 5 0.0 3 0 0 0 2 3 19 85 0.0 3 0 54 19 9 3 20 6146 0.0 4 5356 673 74 33 10 21 32230 0.0 4 30599 1316 240 52 23 22 80 0.0 4 19 47 9 2 3 23 20 0.0 4 1 1 4 9 5 24 112 0.0 4 0 0 0 89 23 25 5 0.0 4 1 0 0 0 4 30 1 0.0 4 0 0 0 0 1 37 1 0.0 4 1 43 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 0 0 0 1 49 1 0.0 4 0 0 0 0 1 54 1 0.0 4 0 0 0 0 1 78 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 92 4 0.0 4 0 0 0 0 4 93 3 0.0 4 0 0 0 0 3 102 1 0.0 4 0 0 0 0 1 111 1 0.0 4 0 0 0 0 1 112 4 0.0 4 0 0 0 0 4 114 1 0.0 4 0 0 0 0 1 115 3 0.0 4 0 0 0 0 3 125 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 109 0.0 4 0 0 0 0 109 128 161 0.0 4 0 0 0 0 161 129 2 0.0 4 0 0 0 0 2 132 1 0.0 4 0 0 0 0 1 133 1 0.0 4 0 0 0 0 1 139 1 0.0 4 0 0 0 0 1 140 9 0.0 4 0 0 0 0 9 141 2 0.0 4 0 0 0 0 2 142 8 0.0 4 0 0 0 0 8 143 2 0.0 4 0 0 0 0 2 147 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 154 5 0.0 4 0 0 0 0 5 163 1 0.0 4 0 0 0 0 1 164 1 0.0 4 0 0 0 0 1 165 2 0.0 4 0 0 0 0 2 171 16 0.0 4 0 0 0 0 16 172 1 0.0 4 0 0 0 0 1 173 3 0.0 4 0 0 0 0 3 175 1 0.0 4 0 0 0 0 1 177 1 0.0 4 0 0 0 0 1 218 1 0.0 4 0 0 0 0 1 222 1 0.0 4 0 0 0 0 1 224 1 0.0 4 0 0 0 0 1 229 2 0.0 4 0 0 0 0 2 230 2 0.0 4 0 0 0 0 2 234 3 0.0 4 0 0 0 0 3 236 1 0.0 4 0 0 0 0 1 238 1 0.0 4 0 0 0 0 1 244 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 265 1 0.0 4 0 0 0 0 1 266 1 0.0 4 0 0 0 0 1 267 1 0.0 4 0 0 0 0 1 288 1 0.0 4 0 0 0 0 1 293 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.28 s (59 us/read; 1.03 M reads/minute). === Summary === Total reads processed: 38,970 Reads with adapters: 10,351 (26.6%) Reads written (passing filters): 38,970 (100.0%) Total basepairs processed: 10,577,155 bp Total written (filtered): 10,464,277 bp (98.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 10351 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.6% C: 5.1% G: 91.8% T: 1.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 11 608.9 0 11 4 5 152.2 0 5 5 1 38.1 0 1 7 1 2.4 0 1 8 3 0.6 0 3 9 4311 0.1 0 341 3970 10 3656 0.0 1 230 3426 11 2269 0.0 1 42 2227 12 19 0.0 1 1 18 13 14 0.0 1 0 14 14 1 0.0 1 0 1 139 1 0.0 1 1 150 7 0.0 1 5 2 151 1 0.0 1 1 189 2 0.0 1 0 2 208 39 0.0 1 34 5 210 1 0.0 1 1 223 9 0.0 1 9 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.