This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CATTAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 64.19 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 60,312 (2.5%) Reads that were too short: 12,240 (0.5%) Reads written (passing filters): 48,072 (2.0%) Total basepairs processed: 740,966,583 bp Total written (filtered): 9,138,215 bp (1.2%) === Adapter 1 === Sequence: CATTAG; Type: regular 5'; Length: 6; Trimmed: 60312 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 212 601.0 0 212 7 2326 601.0 0 2326 8 2042 601.0 0 2042 9 173 601.0 0 173 10 67 601.0 0 67 11 82 601.0 0 82 12 73 601.0 0 73 13 65 601.0 0 65 14 85 601.0 0 85 15 79 601.0 0 79 16 55 601.0 0 55 17 87 601.0 0 87 18 72 601.0 0 72 19 60 601.0 0 60 20 101 601.0 0 101 21 103 601.0 0 103 22 61 601.0 0 61 23 71 601.0 0 71 24 82 601.0 0 82 25 112 601.0 0 112 26 107 601.0 0 107 27 343 601.0 0 343 28 188 601.0 0 188 29 167 601.0 0 167 30 65 601.0 0 65 31 70 601.0 0 70 32 63 601.0 0 63 33 88 601.0 0 88 34 74 601.0 0 74 35 108 601.0 0 108 36 93 601.0 0 93 37 114 601.0 0 114 38 73 601.0 0 73 39 71 601.0 0 71 40 66 601.0 0 66 41 73 601.0 0 73 42 61 601.0 0 61 43 58 601.0 0 58 44 80 601.0 0 80 45 80 601.0 0 80 46 89 601.0 0 89 47 80 601.0 0 80 48 79 601.0 0 79 49 69 601.0 0 69 50 89 601.0 0 89 51 98 601.0 0 98 52 92 601.0 0 92 53 85 601.0 0 85 54 79 601.0 0 79 55 68 601.0 0 68 56 78 601.0 0 78 57 99 601.0 0 99 58 241 601.0 0 241 59 432 601.0 0 432 60 206 601.0 0 206 61 71 601.0 0 71 62 91 601.0 0 91 63 67 601.0 0 67 64 65 601.0 0 65 65 73 601.0 0 73 66 74 601.0 0 74 67 60 601.0 0 60 68 96 601.0 0 96 69 109 601.0 0 109 70 163 601.0 0 163 71 244 601.0 0 244 72 1831 601.0 0 1831 73 476 601.0 0 476 74 579 601.0 0 579 75 72 601.0 0 72 76 68 601.0 0 68 77 83 601.0 0 83 78 83 601.0 0 83 79 85 601.0 0 85 80 79 601.0 0 79 81 70 601.0 0 70 82 64 601.0 0 64 83 69 601.0 0 69 84 85 601.0 0 85 85 62 601.0 0 62 86 72 601.0 0 72 87 81 601.0 0 81 88 102 601.0 0 102 89 135 601.0 0 135 90 462 601.0 0 462 91 562 601.0 0 562 92 346 601.0 0 346 93 78 601.0 0 78 94 73 601.0 0 73 95 68 601.0 0 68 96 104 601.0 0 104 97 69 601.0 0 69 98 55 601.0 0 55 99 78 601.0 0 78 100 83 601.0 0 83 101 79 601.0 0 79 102 76 601.0 0 76 103 68 601.0 0 68 104 74 601.0 0 74 105 56 601.0 0 56 106 59 601.0 0 59 107 62 601.0 0 62 108 81 601.0 0 81 109 109 601.0 0 109 110 115 601.0 0 115 111 77 601.0 0 77 112 401 601.0 0 401 113 232 601.0 0 232 114 308 601.0 0 308 115 68 601.0 0 68 116 60 601.0 0 60 117 75 601.0 0 75 118 50 601.0 0 50 119 82 601.0 0 82 120 96 601.0 0 96 121 93 601.0 0 93 122 67 601.0 0 67 123 81 601.0 0 81 124 75 601.0 0 75 125 80 601.0 0 80 126 69 601.0 0 69 127 99 601.0 0 99 128 116 601.0 0 116 129 181 601.0 0 181 130 284 601.0 0 284 131 162 601.0 0 162 132 101 601.0 0 101 133 137 601.0 0 137 134 146 601.0 0 146 135 155 601.0 0 155 136 220 601.0 0 220 137 131 601.0 0 131 138 133 601.0 0 133 139 148 601.0 0 148 140 203 601.0 0 203 141 282 601.0 0 282 142 497 601.0 0 497 143 6567 601.0 0 6567 144 6787 601.0 0 6787 145 2876 601.0 0 2876 146 110 601.0 0 110 147 89 601.0 0 89 148 126 601.0 0 126 149 137 601.0 0 137 150 102 601.0 0 102 151 70 601.0 0 70 152 117 601.0 0 117 153 92 601.0 0 92 154 117 601.0 0 117 155 1257 601.0 0 1257 156 1216 601.0 0 1216 157 1324 601.0 0 1324 158 102 601.0 0 102 159 92 601.0 0 92 160 66 601.0 0 66 161 66 601.0 0 66 162 106 601.0 0 106 163 463 601.0 0 463 164 305 601.0 0 305 165 354 601.0 0 354 166 81 601.0 0 81 167 92 601.0 0 92 168 92 601.0 0 92 169 87 601.0 0 87 170 80 601.0 0 80 171 64 601.0 0 64 172 74 601.0 0 74 173 87 601.0 0 87 174 65 601.0 0 65 175 52 601.0 0 52 176 64 601.0 0 64 177 72 601.0 0 72 178 46 601.0 0 46 179 70 601.0 0 70 180 66 601.0 0 66 181 55 601.0 0 55 182 98 601.0 0 98 183 66 601.0 0 66 184 78 601.0 0 78 185 72 601.0 0 72 186 67 601.0 0 67 187 74 601.0 0 74 188 99 601.0 0 99 189 70 601.0 0 70 190 68 601.0 0 68 191 80 601.0 0 80 192 115 601.0 0 115 193 75 601.0 0 75 194 64 601.0 0 64 195 85 601.0 0 85 196 105 601.0 0 105 197 65 601.0 0 65 198 86 601.0 0 86 199 66 601.0 0 66 200 66 601.0 0 66 201 76 601.0 0 76 202 53 601.0 0 53 203 89 601.0 0 89 204 88 601.0 0 88 205 90 601.0 0 90 206 94 601.0 0 94 207 86 601.0 0 86 208 134 601.0 0 134 209 137 601.0 0 137 210 151 601.0 0 151 211 273 601.0 0 273 212 130 601.0 0 130 213 129 601.0 0 129 214 131 601.0 0 131 215 294 601.0 0 294 216 565 601.0 0 565 217 397 601.0 0 397 218 68 601.0 0 68 219 58 601.0 0 58 220 70 601.0 0 70 221 92 601.0 0 92 222 100 601.0 0 100 223 96 601.0 0 96 224 100 601.0 0 100 225 104 601.0 0 104 226 106 601.0 0 106 227 114 601.0 0 114 228 70 601.0 0 70 229 70 601.0 0 70 230 69 601.0 0 69 231 202 601.0 0 202 232 176 601.0 0 176 233 186 601.0 0 186 234 133 601.0 0 133 235 132 601.0 0 132 236 110 601.0 0 110 237 88 601.0 0 88 238 63 601.0 0 63 239 116 601.0 0 116 240 77 601.0 0 77 241 85 601.0 0 85 242 77 601.0 0 77 243 84 601.0 0 84 244 91 601.0 0 91 245 144 601.0 0 144 246 149 601.0 0 149 247 76 601.0 0 76 248 57 601.0 0 57 249 61 601.0 0 61 250 55 601.0 0 55 251 68 601.0 0 68 252 72 601.0 0 72 253 94 601.0 0 94 254 107 601.0 0 107 255 114 601.0 0 114 256 83 601.0 0 83 257 127 601.0 0 127 258 92 601.0 0 92 259 131 601.0 0 131 260 104 601.0 0 104 261 76 601.0 0 76 262 63 601.0 0 63 263 149 601.0 0 149 264 204 601.0 0 204 265 144 601.0 0 144 266 114 601.0 0 114 267 74 601.0 0 74 268 83 601.0 0 83 269 104 601.0 0 104 270 100 601.0 0 100 271 90 601.0 0 90 272 88 601.0 0 88 273 86 601.0 0 86 274 84 601.0 0 84 275 89 601.0 0 89 276 84 601.0 0 84 277 91 601.0 0 91 278 85 601.0 0 85 279 104 601.0 0 104 280 231 601.0 0 231 281 220 601.0 0 220 282 160 601.0 0 160 283 117 601.0 0 117 284 152 601.0 0 152 285 162 601.0 0 162 286 144 601.0 0 144 287 89 601.0 0 89 288 80 601.0 0 80 289 84 601.0 0 84 290 90 601.0 0 90 291 98 601.0 0 98 292 109 601.0 0 109 293 91 601.0 0 91 294 120 601.0 0 120 295 292 601.0 0 292 296 312 601.0 0 312 297 274 601.0 0 274 298 116 601.0 0 116 299 91 601.0 0 91 300 89 601.0 0 89 301 95 601.0 0 95 Finished in 2.05 s (43 us/read; 1.41 M reads/minute). === Summary === Total reads processed: 48,072 Reads with adapters: 2,055 (4.3%) Reads that were too short: 21 (0.0%) Reads written (passing filters): 2,034 (4.2%) Total basepairs processed: 9,138,215 bp Total written (filtered): 554,737 bp (6.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 2055 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 2 0.0 3 0 0 0 0 2 19 33 0.0 3 0 25 3 5 20 1952 0.0 4 1773 134 37 7 1 21 10 0.0 4 6 4 22 20 0.0 4 15 5 23 10 0.0 4 1 0 0 6 3 24 1 0.0 4 0 0 0 0 1 27 1 0.0 4 0 0 0 1 45 1 0.0 4 0 0 0 0 1 48 1 0.0 4 0 0 0 0 1 51 1 0.0 4 0 0 0 0 1 71 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 0 0 0 1 87 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 0 1 96 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 101 2 0.0 4 0 0 0 0 2 109 2 0.0 4 0 0 0 0 2 113 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 0 1 122 1 0.0 4 0 0 0 0 1 133 1 0.0 4 0 0 0 0 1 134 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 0 0 0 1 145 1 0.0 4 0 0 0 0 1 146 2 0.0 4 0 0 0 0 2 151 1 0.0 4 0 0 0 0 1 160 1 0.0 4 0 0 0 0 1 172 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.08 s (39 us/read; 1.53 M reads/minute). === Summary === Total reads processed: 2,034 Reads with adapters: 424 (20.8%) Reads written (passing filters): 2,034 (100.0%) Total basepairs processed: 554,737 bp Total written (filtered): 549,764 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 424 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.6% C: 11.8% G: 82.8% T: 2.8% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 2 31.8 0 2 4 1 7.9 0 1 6 1 0.5 0 1 9 5 0.0 0 1 4 10 38 0.0 1 6 32 11 372 0.0 1 17 355 12 2 0.0 1 0 2 14 1 0.0 1 0 1 191 1 0.0 1 0 1 211 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.