This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CATTAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 63.67 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 58,528 (2.4%) Reads that were too short: 10,710 (0.4%) Reads written (passing filters): 47,818 (1.9%) Total basepairs processed: 740,966,583 bp Total written (filtered): 9,103,467 bp (1.2%) === Adapter 1 === Sequence: CATTAG; Type: regular 5'; Length: 6; Trimmed: 58528 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 148 601.0 0 148 7 2322 601.0 0 2322 8 2306 601.0 0 2306 9 127 601.0 0 127 10 65 601.0 0 65 11 90 601.0 0 90 12 85 601.0 0 85 13 67 601.0 0 67 14 105 601.0 0 105 15 84 601.0 0 84 16 64 601.0 0 64 17 100 601.0 0 100 18 94 601.0 0 94 19 73 601.0 0 73 20 101 601.0 0 101 21 111 601.0 0 111 22 69 601.0 0 69 23 92 601.0 0 92 24 86 601.0 0 86 25 77 601.0 0 77 26 105 601.0 0 105 27 121 601.0 0 121 28 95 601.0 0 95 29 72 601.0 0 72 30 85 601.0 0 85 31 91 601.0 0 91 32 93 601.0 0 93 33 83 601.0 0 83 34 71 601.0 0 71 35 106 601.0 0 106 36 109 601.0 0 109 37 109 601.0 0 109 38 91 601.0 0 91 39 107 601.0 0 107 40 85 601.0 0 85 41 83 601.0 0 83 42 83 601.0 0 83 43 83 601.0 0 83 44 93 601.0 0 93 45 102 601.0 0 102 46 75 601.0 0 75 47 82 601.0 0 82 48 95 601.0 0 95 49 86 601.0 0 86 50 104 601.0 0 104 51 96 601.0 0 96 52 101 601.0 0 101 53 96 601.0 0 96 54 134 601.0 0 134 55 99 601.0 0 99 56 112 601.0 0 112 57 147 601.0 0 147 58 188 601.0 0 188 59 487 601.0 0 487 60 221 601.0 0 221 61 94 601.0 0 94 62 112 601.0 0 112 63 100 601.0 0 100 64 70 601.0 0 70 65 94 601.0 0 94 66 77 601.0 0 77 67 92 601.0 0 92 68 95 601.0 0 95 69 87 601.0 0 87 70 124 601.0 0 124 71 210 601.0 0 210 72 1782 601.0 0 1782 73 533 601.0 0 533 74 587 601.0 0 587 75 83 601.0 0 83 76 66 601.0 0 66 77 90 601.0 0 90 78 104 601.0 0 104 79 80 601.0 0 80 80 72 601.0 0 72 81 106 601.0 0 106 82 78 601.0 0 78 83 99 601.0 0 99 84 81 601.0 0 81 85 79 601.0 0 79 86 95 601.0 0 95 87 98 601.0 0 98 88 108 601.0 0 108 89 146 601.0 0 146 90 320 601.0 0 320 91 315 601.0 0 315 92 229 601.0 0 229 93 142 601.0 0 142 94 85 601.0 0 85 95 88 601.0 0 88 96 112 601.0 0 112 97 89 601.0 0 89 98 69 601.0 0 69 99 110 601.0 0 110 100 99 601.0 0 99 101 76 601.0 0 76 102 120 601.0 0 120 103 83 601.0 0 83 104 105 601.0 0 105 105 97 601.0 0 97 106 90 601.0 0 90 107 103 601.0 0 103 108 105 601.0 0 105 109 111 601.0 0 111 110 107 601.0 0 107 111 87 601.0 0 87 112 154 601.0 0 154 113 128 601.0 0 128 114 153 601.0 0 153 115 68 601.0 0 68 116 88 601.0 0 88 117 67 601.0 0 67 118 57 601.0 0 57 119 111 601.0 0 111 120 116 601.0 0 116 121 100 601.0 0 100 122 81 601.0 0 81 123 105 601.0 0 105 124 114 601.0 0 114 125 92 601.0 0 92 126 80 601.0 0 80 127 86 601.0 0 86 128 119 601.0 0 119 129 158 601.0 0 158 130 204 601.0 0 204 131 171 601.0 0 171 132 110 601.0 0 110 133 152 601.0 0 152 134 174 601.0 0 174 135 131 601.0 0 131 136 227 601.0 0 227 137 126 601.0 0 126 138 153 601.0 0 153 139 132 601.0 0 132 140 186 601.0 0 186 141 228 601.0 0 228 142 384 601.0 0 384 143 6133 601.0 0 6133 144 6157 601.0 0 6157 145 2686 601.0 0 2686 146 97 601.0 0 97 147 111 601.0 0 111 148 123 601.0 0 123 149 152 601.0 0 152 150 107 601.0 0 107 151 112 601.0 0 112 152 132 601.0 0 132 153 111 601.0 0 111 154 110 601.0 0 110 155 1540 601.0 0 1540 156 1424 601.0 0 1424 157 1597 601.0 0 1597 158 124 601.0 0 124 159 97 601.0 0 97 160 81 601.0 0 81 161 99 601.0 0 99 162 127 601.0 0 127 163 386 601.0 0 386 164 234 601.0 0 234 165 317 601.0 0 317 166 104 601.0 0 104 167 91 601.0 0 91 168 79 601.0 0 79 169 83 601.0 0 83 170 112 601.0 0 112 171 92 601.0 0 92 172 62 601.0 0 62 173 81 601.0 0 81 174 83 601.0 0 83 175 92 601.0 0 92 176 93 601.0 0 93 177 79 601.0 0 79 178 75 601.0 0 75 179 84 601.0 0 84 180 103 601.0 0 103 181 71 601.0 0 71 182 84 601.0 0 84 183 77 601.0 0 77 184 91 601.0 0 91 185 73 601.0 0 73 186 67 601.0 0 67 187 72 601.0 0 72 188 83 601.0 0 83 189 91 601.0 0 91 190 82 601.0 0 82 191 89 601.0 0 89 192 83 601.0 0 83 193 97 601.0 0 97 194 70 601.0 0 70 195 91 601.0 0 91 196 107 601.0 0 107 197 105 601.0 0 105 198 77 601.0 0 77 199 73 601.0 0 73 200 91 601.0 0 91 201 79 601.0 0 79 202 81 601.0 0 81 203 76 601.0 0 76 204 86 601.0 0 86 205 78 601.0 0 78 206 117 601.0 0 117 207 107 601.0 0 107 208 114 601.0 0 114 209 159 601.0 0 159 210 154 601.0 0 154 211 91 601.0 0 91 212 90 601.0 0 90 213 110 601.0 0 110 214 104 601.0 0 104 215 228 601.0 0 228 216 527 601.0 0 527 217 333 601.0 0 333 218 79 601.0 0 79 219 72 601.0 0 72 220 103 601.0 0 103 221 89 601.0 0 89 222 93 601.0 0 93 223 83 601.0 0 83 224 103 601.0 0 103 225 98 601.0 0 98 226 98 601.0 0 98 227 111 601.0 0 111 228 93 601.0 0 93 229 70 601.0 0 70 230 86 601.0 0 86 231 102 601.0 0 102 232 90 601.0 0 90 233 104 601.0 0 104 234 108 601.0 0 108 235 70 601.0 0 70 236 100 601.0 0 100 237 103 601.0 0 103 238 77 601.0 0 77 239 85 601.0 0 85 240 80 601.0 0 80 241 88 601.0 0 88 242 83 601.0 0 83 243 64 601.0 0 64 244 91 601.0 0 91 245 123 601.0 0 123 246 98 601.0 0 98 247 83 601.0 0 83 248 83 601.0 0 83 249 96 601.0 0 96 250 67 601.0 0 67 251 78 601.0 0 78 252 88 601.0 0 88 253 96 601.0 0 96 254 96 601.0 0 96 255 110 601.0 0 110 256 67 601.0 0 67 257 121 601.0 0 121 258 96 601.0 0 96 259 115 601.0 0 115 260 112 601.0 0 112 261 103 601.0 0 103 262 84 601.0 0 84 263 105 601.0 0 105 264 114 601.0 0 114 265 95 601.0 0 95 266 78 601.0 0 78 267 106 601.0 0 106 268 88 601.0 0 88 269 104 601.0 0 104 270 84 601.0 0 84 271 100 601.0 0 100 272 101 601.0 0 101 273 102 601.0 0 102 274 66 601.0 0 66 275 81 601.0 0 81 276 88 601.0 0 88 277 92 601.0 0 92 278 114 601.0 0 114 279 97 601.0 0 97 280 161 601.0 0 161 281 173 601.0 0 173 282 135 601.0 0 135 283 102 601.0 0 102 284 114 601.0 0 114 285 109 601.0 0 109 286 100 601.0 0 100 287 89 601.0 0 89 288 91 601.0 0 91 289 87 601.0 0 87 290 113 601.0 0 113 291 110 601.0 0 110 292 96 601.0 0 96 293 105 601.0 0 105 294 80 601.0 0 80 295 149 601.0 0 149 296 168 601.0 0 168 297 160 601.0 0 160 298 86 601.0 0 86 299 100 601.0 0 100 300 92 601.0 0 92 301 81 601.0 0 81 Finished in 1.75 s (37 us/read; 1.64 M reads/minute). === Summary === Total reads processed: 47,818 Reads with adapters: 2,209 (4.6%) Reads that were too short: 38 (0.1%) Reads written (passing filters): 2,171 (4.5%) Total basepairs processed: 9,103,467 bp Total written (filtered): 595,842 bp (6.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 2209 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 12 0.0 3 0 11 0 1 17 2114 0.0 3 2069 42 3 18 4 0.0 3 0 4 19 6 0.0 3 4 0 2 20 1 0.0 3 0 0 0 1 24 1 0.0 3 0 0 0 1 28 5 0.0 3 0 0 0 5 30 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 36 2 0.0 3 0 0 0 2 48 5 0.0 3 0 0 0 5 51 4 0.0 3 0 0 0 4 59 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 79 2 0.0 3 0 0 0 2 81 1 0.0 3 0 0 0 1 90 2 0.0 3 0 0 0 2 95 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 106 3 0.0 3 0 0 0 3 124 1 0.0 3 0 0 0 1 135 22 0.0 3 0 0 0 22 138 2 0.0 3 0 0 0 2 150 4 0.0 3 0 0 0 4 163 1 0.0 3 0 0 0 1 164 2 0.0 3 0 0 0 2 183 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 271 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.08 s (37 us/read; 1.63 M reads/minute). === Summary === Total reads processed: 2,171 Reads with adapters: 14 (0.6%) Reads written (passing filters): 2,171 (100.0%) Total basepairs processed: 595,842 bp Total written (filtered): 595,800 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 14 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 71.4% C: 0.0% G: 14.3% T: 14.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 14 33.9 0 14