This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCACT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 67.61 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 366,069 (14.4%) Reads that were too short: 20,082 (0.8%) Reads written (passing filters): 345,987 (13.6%) Total basepairs processed: 766,381,518 bp Total written (filtered): 67,232,255 bp (8.8%) === Adapter 1 === Sequence: AGCACT; Type: regular 5'; Length: 6; Trimmed: 366069 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 96 621.6 0 96 7 2309 621.6 0 2309 8 1969 621.6 0 1969 9 73 621.6 0 73 10 55 621.6 0 55 11 68 621.6 0 68 12 186 621.6 0 186 13 234 621.6 0 234 14 292 621.6 0 292 15 73 621.6 0 73 16 52 621.6 0 52 17 63 621.6 0 63 18 74 621.6 0 74 19 86 621.6 0 86 20 145 621.6 0 145 21 148 621.6 0 148 22 134 621.6 0 134 23 102 621.6 0 102 24 95 621.6 0 95 25 435 621.6 0 435 26 285 621.6 0 285 27 120 621.6 0 120 28 73 621.6 0 73 29 108 621.6 0 108 30 93 621.6 0 93 31 86 621.6 0 86 32 108 621.6 0 108 33 66 621.6 0 66 34 83 621.6 0 83 35 133 621.6 0 133 36 142 621.6 0 142 37 235 621.6 0 235 38 91 621.6 0 91 39 107 621.6 0 107 40 87 621.6 0 87 41 75 621.6 0 75 42 83 621.6 0 83 43 141 621.6 0 141 44 103 621.6 0 103 45 48 621.6 0 48 46 87 621.6 0 87 47 82 621.6 0 82 48 80 621.6 0 80 49 74 621.6 0 74 50 74 621.6 0 74 51 85 621.6 0 85 52 96 621.6 0 96 53 70 621.6 0 70 54 109 621.6 0 109 55 118 621.6 0 118 56 129 621.6 0 129 57 91 621.6 0 91 58 118 621.6 0 118 59 97 621.6 0 97 60 95 621.6 0 95 61 111 621.6 0 111 62 155 621.6 0 155 63 125 621.6 0 125 64 147 621.6 0 147 65 1359 621.6 0 1359 66 723 621.6 0 723 67 631 621.6 0 631 68 86 621.6 0 86 69 119 621.6 0 119 70 101 621.6 0 101 71 85 621.6 0 85 72 125 621.6 0 125 73 79 621.6 0 79 74 109 621.6 0 109 75 112 621.6 0 112 76 111 621.6 0 111 77 149 621.6 0 149 78 352 621.6 0 352 79 211 621.6 0 211 80 740 621.6 0 740 81 267 621.6 0 267 82 193 621.6 0 193 83 111 621.6 0 111 84 170 621.6 0 170 85 150 621.6 0 150 86 253 621.6 0 253 87 298 621.6 0 298 88 431 621.6 0 431 89 638 621.6 0 638 90 1425 621.6 0 1425 91 2070 621.6 0 2070 92 422 621.6 0 422 93 611 621.6 0 611 94 1118 621.6 0 1118 95 528 621.6 0 528 96 430 621.6 0 430 97 835 621.6 0 835 98 930 621.6 0 930 99 1361 621.6 0 1361 100 1270 621.6 0 1270 101 1770 621.6 0 1770 102 3333 621.6 0 3333 103 6739 621.6 0 6739 104 130637 621.6 0 130637 105 65023 621.6 0 65023 106 73090 621.6 0 73090 107 319 621.6 0 319 108 63 621.6 0 63 109 62 621.6 0 62 110 141 621.6 0 141 111 186 621.6 0 186 112 172 621.6 0 172 113 94 621.6 0 94 114 101 621.6 0 101 115 95 621.6 0 95 116 93 621.6 0 93 117 121 621.6 0 121 118 80 621.6 0 80 119 163 621.6 0 163 120 107 621.6 0 107 121 161 621.6 0 161 122 83 621.6 0 83 123 109 621.6 0 109 124 509 621.6 0 509 125 392 621.6 0 392 126 296 621.6 0 296 127 171 621.6 0 171 128 244 621.6 0 244 129 63 621.6 0 63 130 55 621.6 0 55 131 71 621.6 0 71 132 120 621.6 0 120 133 67 621.6 0 67 134 80 621.6 0 80 135 56 621.6 0 56 136 60 621.6 0 60 137 89 621.6 0 89 138 188 621.6 0 188 139 188 621.6 0 188 140 225 621.6 0 225 141 80 621.6 0 80 142 135 621.6 0 135 143 71 621.6 0 71 144 112 621.6 0 112 145 88 621.6 0 88 146 140 621.6 0 140 147 154 621.6 0 154 148 145 621.6 0 145 149 101 621.6 0 101 150 96 621.6 0 96 151 254 621.6 0 254 152 230 621.6 0 230 153 402 621.6 0 402 154 193 621.6 0 193 155 205 621.6 0 205 156 233 621.6 0 233 157 484 621.6 0 484 158 980 621.6 0 980 159 10168 621.6 0 10168 160 3850 621.6 0 3850 161 6598 621.6 0 6598 162 97 621.6 0 97 163 91 621.6 0 91 164 95 621.6 0 95 165 88 621.6 0 88 166 120 621.6 0 120 167 435 621.6 0 435 168 278 621.6 0 278 169 252 621.6 0 252 170 80 621.6 0 80 171 171 621.6 0 171 172 282 621.6 0 282 173 329 621.6 0 329 174 211 621.6 0 211 175 74 621.6 0 74 176 88 621.6 0 88 177 99 621.6 0 99 178 77 621.6 0 77 179 81 621.6 0 81 180 111 621.6 0 111 181 113 621.6 0 113 182 100 621.6 0 100 183 118 621.6 0 118 184 354 621.6 0 354 185 166 621.6 0 166 186 284 621.6 0 284 187 85 621.6 0 85 188 103 621.6 0 103 189 107 621.6 0 107 190 72 621.6 0 72 191 75 621.6 0 75 192 87 621.6 0 87 193 96 621.6 0 96 194 99 621.6 0 99 195 71 621.6 0 71 196 95 621.6 0 95 197 90 621.6 0 90 198 119 621.6 0 119 199 151 621.6 0 151 200 433 621.6 0 433 201 273 621.6 0 273 202 418 621.6 0 418 203 93 621.6 0 93 204 104 621.6 0 104 205 167 621.6 0 167 206 194 621.6 0 194 207 189 621.6 0 189 208 134 621.6 0 134 209 131 621.6 0 131 210 72 621.6 0 72 211 76 621.6 0 76 212 72 621.6 0 72 213 80 621.6 0 80 214 99 621.6 0 99 215 120 621.6 0 120 216 87 621.6 0 87 217 102 621.6 0 102 218 146 621.6 0 146 219 244 621.6 0 244 220 242 621.6 0 242 221 169 621.6 0 169 222 95 621.6 0 95 223 103 621.6 0 103 224 110 621.6 0 110 225 97 621.6 0 97 226 226 621.6 0 226 227 142 621.6 0 142 228 291 621.6 0 291 229 180 621.6 0 180 230 196 621.6 0 196 231 137 621.6 0 137 232 108 621.6 0 108 233 132 621.6 0 132 234 128 621.6 0 128 235 91 621.6 0 91 236 215 621.6 0 215 237 152 621.6 0 152 238 289 621.6 0 289 239 261 621.6 0 261 240 1286 621.6 0 1286 241 1156 621.6 0 1156 242 1274 621.6 0 1274 243 419 621.6 0 419 244 94 621.6 0 94 245 91 621.6 0 91 246 88 621.6 0 88 247 129 621.6 0 129 248 111 621.6 0 111 249 103 621.6 0 103 250 97 621.6 0 97 251 127 621.6 0 127 252 117 621.6 0 117 253 144 621.6 0 144 254 297 621.6 0 297 255 437 621.6 0 437 256 524 621.6 0 524 257 340 621.6 0 340 258 92 621.6 0 92 259 87 621.6 0 87 260 90 621.6 0 90 261 104 621.6 0 104 262 93 621.6 0 93 263 105 621.6 0 105 264 85 621.6 0 85 265 109 621.6 0 109 266 103 621.6 0 103 267 160 621.6 0 160 268 186 621.6 0 186 269 193 621.6 0 193 270 139 621.6 0 139 271 120 621.6 0 120 272 103 621.6 0 103 273 155 621.6 0 155 274 239 621.6 0 239 275 203 621.6 0 203 276 212 621.6 0 212 277 123 621.6 0 123 278 218 621.6 0 218 279 272 621.6 0 272 280 264 621.6 0 264 281 329 621.6 0 329 282 290 621.6 0 290 283 458 621.6 0 458 284 172 621.6 0 172 285 96 621.6 0 96 286 100 621.6 0 100 287 92 621.6 0 92 288 137 621.6 0 137 289 105 621.6 0 105 290 122 621.6 0 122 291 118 621.6 0 118 292 139 621.6 0 139 293 166 621.6 0 166 294 198 621.6 0 198 295 127 621.6 0 127 296 147 621.6 0 147 297 99 621.6 0 99 298 110 621.6 0 110 299 211 621.6 0 211 300 356 621.6 0 356 301 329 621.6 0 329 Finished in 13.80 s (40 us/read; 1.50 M reads/minute). === Summary === Total reads processed: 345,987 Reads with adapters: 8,187 (2.4%) Reads that were too short: 174 (0.1%) Reads written (passing filters): 8,013 (2.3%) Total basepairs processed: 67,232,255 bp Total written (filtered): 1,548,639 bp (2.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 8187 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 5 0.0 3 0 0 3 1 1 19 31 0.0 3 0 20 4 5 2 20 1923 0.0 4 1763 127 23 6 4 21 5 0.0 4 0 3 1 0 1 22 2 0.0 4 1 1 23 12 0.0 4 1 0 1 10 26 1 0.0 4 0 0 0 0 1 27 1 0.0 4 0 0 0 0 1 29 4 0.0 4 0 0 0 0 4 30 5550 0.0 4 0 0 0 0 5550 31 1 0.0 4 0 0 0 0 1 32 19 0.0 4 0 0 0 0 19 33 22 0.0 4 0 0 0 1 21 34 1 0.0 4 0 0 0 0 1 37 1 0.0 4 1 43 1 0.0 4 0 0 0 0 1 55 1 0.0 4 0 0 0 0 1 56 1 0.0 4 0 0 0 0 1 64 1 0.0 4 0 1 69 4 0.0 4 0 0 0 0 4 70 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 1 100 1 0.0 4 0 0 0 0 1 108 3 0.0 4 0 0 0 0 3 109 1 0.0 4 0 0 0 0 1 110 3 0.0 4 0 0 0 0 3 111 2 0.0 4 0 0 0 0 2 112 1 0.0 4 0 0 0 0 1 118 1 0.0 4 0 0 0 0 1 119 1 0.0 4 0 0 0 0 1 125 2 0.0 4 0 0 0 0 2 126 6 0.0 4 0 0 0 0 6 127 414 0.0 4 0 0 0 0 414 128 6 0.0 4 0 0 0 0 6 129 5 0.0 4 0 0 0 0 5 136 6 0.0 4 0 0 0 0 6 137 21 0.0 4 0 0 0 1 20 138 15 0.0 4 0 0 0 1 14 139 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 141 1 0.0 4 0 0 0 0 1 143 1 0.0 4 0 0 0 0 1 151 1 0.0 4 0 0 0 0 1 156 2 0.0 4 0 0 0 0 2 160 1 0.0 4 0 0 0 0 1 161 1 0.0 4 0 0 0 0 1 162 2 0.0 4 0 0 0 0 2 165 1 0.0 4 0 0 0 0 1 166 2 0.0 4 0 0 0 0 2 167 1 0.0 4 0 0 0 0 1 170 1 0.0 4 0 0 0 0 1 173 1 0.0 4 0 0 0 0 1 176 1 0.0 4 0 0 0 0 1 185 51 0.0 4 0 0 0 0 51 186 12 0.0 4 0 0 0 1 11 187 2 0.0 4 0 0 0 0 2 188 13 0.0 4 0 1 0 0 12 196 1 0.0 4 0 0 0 0 1 197 2 0.0 4 0 0 0 0 2 198 1 0.0 4 0 0 0 0 1 199 5 0.0 4 0 0 0 0 5 200 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 224 1 0.0 4 0 0 0 0 1 245 1 0.0 4 0 0 0 1 282 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.23 s (29 us/read; 2.09 M reads/minute). === Summary === Total reads processed: 8,013 Reads with adapters: 587 (7.3%) Reads written (passing filters): 8,013 (100.0%) Total basepairs processed: 1,548,639 bp Total written (filtered): 1,542,455 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 587 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 16.4% C: 9.0% G: 71.2% T: 3.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 65 125.2 0 65 4 41 31.3 0 41 5 3 7.8 0 3 10 4 0.0 1 0 4 11 467 0.0 1 20 447 12 2 0.0 1 0 2 68 1 0.0 1 0 1 93 1 0.0 1 1 139 2 0.0 1 2 170 1 0.0 1 0 1