This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TAATGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 65.20 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 318,412 (12.5%) Reads that were too short: 146,638 (5.8%) Reads written (passing filters): 171,774 (6.7%) Total basepairs processed: 766,381,518 bp Total written (filtered): 32,085,964 bp (4.2%) === Adapter 1 === Sequence: TAATGA; Type: regular 5'; Length: 6; Trimmed: 318412 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 302 621.6 0 302 7 7874 621.6 0 7874 8 6153 621.6 0 6153 9 71 621.6 0 71 10 65 621.6 0 65 11 233 621.6 0 233 12 80 621.6 0 80 13 64 621.6 0 64 14 63 621.6 0 63 15 64 621.6 0 64 16 68 621.6 0 68 17 57 621.6 0 57 18 69 621.6 0 69 19 61 621.6 0 61 20 66 621.6 0 66 21 71 621.6 0 71 22 71 621.6 0 71 23 66 621.6 0 66 24 72 621.6 0 72 25 63 621.6 0 63 26 73 621.6 0 73 27 93 621.6 0 93 28 96 621.6 0 96 29 86 621.6 0 86 30 137 621.6 0 137 31 114 621.6 0 114 32 57 621.6 0 57 33 70 621.6 0 70 34 55 621.6 0 55 35 72 621.6 0 72 36 109 621.6 0 109 37 92 621.6 0 92 38 87 621.6 0 87 39 121 621.6 0 121 40 93 621.6 0 93 41 61 621.6 0 61 42 100 621.6 0 100 43 121 621.6 0 121 44 88 621.6 0 88 45 104 621.6 0 104 46 91 621.6 0 91 47 106 621.6 0 106 48 96 621.6 0 96 49 77 621.6 0 77 50 82 621.6 0 82 51 77 621.6 0 77 52 79 621.6 0 79 53 151 621.6 0 151 54 87 621.6 0 87 55 83 621.6 0 83 56 98 621.6 0 98 57 76 621.6 0 76 58 136 621.6 0 136 59 147 621.6 0 147 60 129 621.6 0 129 61 115 621.6 0 115 62 128 621.6 0 128 63 131 621.6 0 131 64 128 621.6 0 128 65 132 621.6 0 132 66 201 621.6 0 201 67 170 621.6 0 170 68 268 621.6 0 268 69 778 621.6 0 778 70 4937 621.6 0 4937 71 5097 621.6 0 5097 72 3472 621.6 0 3472 73 116 621.6 0 116 74 114 621.6 0 114 75 66 621.6 0 66 76 112 621.6 0 112 77 81 621.6 0 81 78 135 621.6 0 135 79 121 621.6 0 121 80 132 621.6 0 132 81 188 621.6 0 188 82 223 621.6 0 223 83 281 621.6 0 281 84 121 621.6 0 121 85 101 621.6 0 101 86 106 621.6 0 106 87 72 621.6 0 72 88 76 621.6 0 76 89 65 621.6 0 65 90 57 621.6 0 57 91 78 621.6 0 78 92 74 621.6 0 74 93 81 621.6 0 81 94 88 621.6 0 88 95 80 621.6 0 80 96 83 621.6 0 83 97 107 621.6 0 107 98 143 621.6 0 143 99 172 621.6 0 172 100 240 621.6 0 240 101 186 621.6 0 186 102 119 621.6 0 119 103 142 621.6 0 142 104 108 621.6 0 108 105 107 621.6 0 107 106 106 621.6 0 106 107 125 621.6 0 125 108 162 621.6 0 162 109 203 621.6 0 203 110 378 621.6 0 378 111 715 621.6 0 715 112 222 621.6 0 222 113 284 621.6 0 284 114 483 621.6 0 483 115 240 621.6 0 240 116 242 621.6 0 242 117 390 621.6 0 390 118 364 621.6 0 364 119 564 621.6 0 564 120 424 621.6 0 424 121 586 621.6 0 586 122 889 621.6 0 889 123 1554 621.6 0 1554 124 27934 621.6 0 27934 125 17331 621.6 0 17331 126 32000 621.6 0 32000 127 688 621.6 0 688 128 242 621.6 0 242 129 431 621.6 0 431 130 252 621.6 0 252 131 306 621.6 0 306 132 115 621.6 0 115 133 118 621.6 0 118 134 134 621.6 0 134 135 205 621.6 0 205 136 393 621.6 0 393 137 254 621.6 0 254 138 226 621.6 0 226 139 252 621.6 0 252 140 204 621.6 0 204 141 158 621.6 0 158 142 289 621.6 0 289 143 189 621.6 0 189 144 288 621.6 0 288 145 361 621.6 0 361 146 347 621.6 0 347 147 526 621.6 0 526 148 988 621.6 0 988 149 16919 621.6 0 16919 150 8126 621.6 0 8126 151 9777 621.6 0 9777 152 97 621.6 0 97 153 61 621.6 0 61 154 105 621.6 0 105 155 89 621.6 0 89 156 109 621.6 0 109 157 120 621.6 0 120 158 124 621.6 0 124 159 94 621.6 0 94 160 124 621.6 0 124 161 84 621.6 0 84 162 152 621.6 0 152 163 206 621.6 0 206 164 192 621.6 0 192 165 122 621.6 0 122 166 92 621.6 0 92 167 80 621.6 0 80 168 83 621.6 0 83 169 101 621.6 0 101 170 76 621.6 0 76 171 79 621.6 0 79 172 84 621.6 0 84 173 76 621.6 0 76 174 76 621.6 0 76 175 73 621.6 0 73 176 74 621.6 0 74 177 85 621.6 0 85 178 96 621.6 0 96 179 102 621.6 0 102 180 90 621.6 0 90 181 232 621.6 0 232 182 185 621.6 0 185 183 365 621.6 0 365 184 96 621.6 0 96 185 111 621.6 0 111 186 132 621.6 0 132 187 96 621.6 0 96 188 192 621.6 0 192 189 106 621.6 0 106 190 121 621.6 0 121 191 93 621.6 0 93 192 96 621.6 0 96 193 84 621.6 0 84 194 122 621.6 0 122 195 230 621.6 0 230 196 124 621.6 0 124 197 133 621.6 0 133 198 574 621.6 0 574 199 90 621.6 0 90 200 89 621.6 0 89 201 64 621.6 0 64 202 81 621.6 0 81 203 164 621.6 0 164 204 194 621.6 0 194 205 242 621.6 0 242 206 232 621.6 0 232 207 361 621.6 0 361 208 828 621.6 0 828 209 197 621.6 0 197 210 264 621.6 0 264 211 204 621.6 0 204 212 258 621.6 0 258 213 699 621.6 0 699 214 448 621.6 0 448 215 375 621.6 0 375 216 164 621.6 0 164 217 159 621.6 0 159 218 332 621.6 0 332 219 376 621.6 0 376 220 618 621.6 0 618 221 905 621.6 0 905 222 892 621.6 0 892 223 3217 621.6 0 3217 224 6290 621.6 0 6290 225 37203 621.6 0 37203 226 51165 621.6 0 51165 227 33382 621.6 0 33382 228 230 621.6 0 230 229 91 621.6 0 91 230 118 621.6 0 118 231 173 621.6 0 173 232 111 621.6 0 111 233 84 621.6 0 84 234 123 621.6 0 123 235 134 621.6 0 134 236 131 621.6 0 131 237 108 621.6 0 108 238 94 621.6 0 94 239 105 621.6 0 105 240 116 621.6 0 116 241 98 621.6 0 98 242 114 621.6 0 114 243 165 621.6 0 165 244 175 621.6 0 175 245 175 621.6 0 175 246 114 621.6 0 114 247 61 621.6 0 61 248 65 621.6 0 65 249 65 621.6 0 65 250 76 621.6 0 76 251 67 621.6 0 67 252 90 621.6 0 90 253 82 621.6 0 82 254 127 621.6 0 127 255 125 621.6 0 125 256 145 621.6 0 145 257 134 621.6 0 134 258 105 621.6 0 105 259 102 621.6 0 102 260 103 621.6 0 103 261 75 621.6 0 75 262 176 621.6 0 176 263 194 621.6 0 194 264 176 621.6 0 176 265 166 621.6 0 166 266 157 621.6 0 157 267 138 621.6 0 138 268 165 621.6 0 165 269 69 621.6 0 69 270 64 621.6 0 64 271 71 621.6 0 71 272 80 621.6 0 80 273 104 621.6 0 104 274 88 621.6 0 88 275 68 621.6 0 68 276 69 621.6 0 69 277 82 621.6 0 82 278 73 621.6 0 73 279 63 621.6 0 63 280 93 621.6 0 93 281 97 621.6 0 97 282 67 621.6 0 67 283 68 621.6 0 68 284 70 621.6 0 70 285 55 621.6 0 55 286 72 621.6 0 72 287 79 621.6 0 79 288 60 621.6 0 60 289 74 621.6 0 74 290 59 621.6 0 59 291 65 621.6 0 65 292 65 621.6 0 65 293 67 621.6 0 67 294 61 621.6 0 61 295 78 621.6 0 78 296 55 621.6 0 55 297 63 621.6 0 63 298 61 621.6 0 61 299 64 621.6 0 64 300 58 621.6 0 58 301 73 621.6 0 73 Finished in 6.84 s (40 us/read; 1.51 M reads/minute). === Summary === Total reads processed: 171,774 Reads with adapters: 6,464 (3.8%) Reads that were too short: 86 (0.1%) Reads written (passing filters): 6,378 (3.7%) Total basepairs processed: 32,085,964 bp Total written (filtered): 1,730,455 bp (5.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 6464 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 1 0.0 3 0 0 0 1 19 21 0.0 3 0 15 1 3 2 20 1302 0.0 4 1141 130 14 10 7 21 4964 0.0 4 4609 286 51 15 3 22 13 0.0 4 4 8 1 23 7 0.0 4 2 0 0 3 2 24 14 0.0 4 0 0 0 7 7 26 1 0.0 4 0 0 0 0 1 30 1 0.0 4 0 0 0 0 1 39 1 0.0 4 0 0 0 0 1 43 1 0.0 4 0 0 0 0 1 45 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 0 0 0 1 50 1 0.0 4 1 56 1 0.0 4 0 0 0 0 1 59 1 0.0 4 0 0 0 1 71 1 0.0 4 0 0 0 0 1 76 1 0.0 4 0 0 0 0 1 79 3 0.0 4 0 0 0 0 3 83 2 0.0 4 0 0 0 0 2 91 1 0.0 4 0 0 0 0 1 92 5 0.0 4 0 0 0 0 5 93 2 0.0 4 0 0 0 0 2 95 2 0.0 4 0 0 0 0 2 96 2 0.0 4 0 0 0 0 2 103 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 1 109 2 0.0 4 0 0 0 1 1 112 4 0.0 4 0 0 0 0 4 115 2 0.0 4 0 0 0 0 2 116 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 0 0 0 1 121 2 0.0 4 0 0 0 0 2 125 1 0.0 4 0 0 0 0 1 126 2 0.0 4 0 0 0 0 2 127 44 0.0 4 0 0 0 0 44 128 1 0.0 4 0 0 0 0 1 132 1 0.0 4 0 0 0 0 1 134 1 0.0 4 0 0 0 0 1 140 9 0.0 4 0 0 0 1 8 141 3 0.0 4 0 0 0 0 3 142 2 0.0 4 0 0 0 0 2 143 1 0.0 4 0 0 0 0 1 149 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 157 1 0.0 4 0 0 0 0 1 159 1 0.0 4 0 0 0 0 1 163 1 0.0 4 0 0 0 0 1 166 1 0.0 4 0 0 0 0 1 171 18 0.0 4 0 0 0 0 18 173 1 0.0 4 0 0 0 0 1 174 4 0.0 4 0 0 0 0 4 182 1 0.0 4 0 0 0 0 1 192 1 0.0 4 0 0 0 0 1 224 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 270 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.25 s (39 us/read; 1.53 M reads/minute). === Summary === Total reads processed: 6,378 Reads with adapters: 2,227 (34.9%) Reads written (passing filters): 6,378 (100.0%) Total basepairs processed: 1,730,455 bp Total written (filtered): 1,706,183 bp (98.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2227 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.2% C: 6.3% G: 89.9% T: 2.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 2 99.7 0 2 4 1 24.9 0 1 6 1 1.6 0 1 9 1276 0.0 0 82 1194 10 496 0.0 1 32 464 11 425 0.0 1 17 408 12 7 0.0 1 0 7 13 3 0.0 1 0 3 14 2 0.0 1 0 2 71 1 0.0 1 1 150 4 0.0 1 2 2 170 1 0.0 1 1 268 7 0.0 1 6 1 269 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.