This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TAATGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 66.81 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 349,621 (13.7%) Reads that were too short: 180,650 (7.1%) Reads written (passing filters): 168,971 (6.6%) Total basepairs processed: 766,381,518 bp Total written (filtered): 31,446,269 bp (4.1%) === Adapter 1 === Sequence: TAATGA; Type: regular 5'; Length: 6; Trimmed: 349621 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 207 621.6 0 207 7 7496 621.6 0 7496 8 7301 621.6 0 7301 9 44 621.6 0 44 10 43 621.6 0 43 11 66 621.6 0 66 12 49 621.6 0 49 13 45 621.6 0 45 14 46 621.6 0 46 15 43 621.6 0 43 16 41 621.6 0 41 17 55 621.6 0 55 18 38 621.6 0 38 19 35 621.6 0 35 20 69 621.6 0 69 21 51 621.6 0 51 22 52 621.6 0 52 23 47 621.6 0 47 24 50 621.6 0 50 25 37 621.6 0 37 26 44 621.6 0 44 27 53 621.6 0 53 28 64 621.6 0 64 29 35 621.6 0 35 30 82 621.6 0 82 31 47 621.6 0 47 32 40 621.6 0 40 33 47 621.6 0 47 34 54 621.6 0 54 35 53 621.6 0 53 36 89 621.6 0 89 37 62 621.6 0 62 38 63 621.6 0 63 39 103 621.6 0 103 40 79 621.6 0 79 41 39 621.6 0 39 42 79 621.6 0 79 43 106 621.6 0 106 44 71 621.6 0 71 45 63 621.6 0 63 46 65 621.6 0 65 47 48 621.6 0 48 48 74 621.6 0 74 49 71 621.6 0 71 50 70 621.6 0 70 51 64 621.6 0 64 52 46 621.6 0 46 53 99 621.6 0 99 54 51 621.6 0 51 55 64 621.6 0 64 56 69 621.6 0 69 57 62 621.6 0 62 58 116 621.6 0 116 59 94 621.6 0 94 60 90 621.6 0 90 61 87 621.6 0 87 62 93 621.6 0 93 63 94 621.6 0 94 64 81 621.6 0 81 65 93 621.6 0 93 66 161 621.6 0 161 67 121 621.6 0 121 68 190 621.6 0 190 69 568 621.6 0 568 70 4843 621.6 0 4843 71 4674 621.6 0 4674 72 3482 621.6 0 3482 73 76 621.6 0 76 74 86 621.6 0 86 75 38 621.6 0 38 76 56 621.6 0 56 77 51 621.6 0 51 78 48 621.6 0 48 79 64 621.6 0 64 80 69 621.6 0 69 81 143 621.6 0 143 82 124 621.6 0 124 83 234 621.6 0 234 84 92 621.6 0 92 85 73 621.6 0 73 86 51 621.6 0 51 87 52 621.6 0 52 88 53 621.6 0 53 89 62 621.6 0 62 90 72 621.6 0 72 91 80 621.6 0 80 92 50 621.6 0 50 93 61 621.6 0 61 94 75 621.6 0 75 95 76 621.6 0 76 96 65 621.6 0 65 97 81 621.6 0 81 98 128 621.6 0 128 99 93 621.6 0 93 100 176 621.6 0 176 101 123 621.6 0 123 102 105 621.6 0 105 103 94 621.6 0 94 104 83 621.6 0 83 105 93 621.6 0 93 106 106 621.6 0 106 107 116 621.6 0 116 108 128 621.6 0 128 109 177 621.6 0 177 110 356 621.6 0 356 111 671 621.6 0 671 112 184 621.6 0 184 113 235 621.6 0 235 114 464 621.6 0 464 115 195 621.6 0 195 116 206 621.6 0 206 117 403 621.6 0 403 118 377 621.6 0 377 119 474 621.6 0 474 120 345 621.6 0 345 121 553 621.6 0 553 122 742 621.6 0 742 123 1229 621.6 0 1229 124 28082 621.6 0 28082 125 17405 621.6 0 17405 126 33422 621.6 0 33422 127 670 621.6 0 670 128 180 621.6 0 180 129 215 621.6 0 215 130 103 621.6 0 103 131 184 621.6 0 184 132 77 621.6 0 77 133 72 621.6 0 72 134 89 621.6 0 89 135 202 621.6 0 202 136 319 621.6 0 319 137 170 621.6 0 170 138 183 621.6 0 183 139 202 621.6 0 202 140 168 621.6 0 168 141 112 621.6 0 112 142 268 621.6 0 268 143 177 621.6 0 177 144 216 621.6 0 216 145 319 621.6 0 319 146 225 621.6 0 225 147 389 621.6 0 389 148 639 621.6 0 639 149 18122 621.6 0 18122 150 8943 621.6 0 8943 151 10521 621.6 0 10521 152 81 621.6 0 81 153 50 621.6 0 50 154 71 621.6 0 71 155 74 621.6 0 74 156 66 621.6 0 66 157 62 621.6 0 62 158 94 621.6 0 94 159 57 621.6 0 57 160 59 621.6 0 59 161 57 621.6 0 57 162 85 621.6 0 85 163 212 621.6 0 212 164 71 621.6 0 71 165 116 621.6 0 116 166 67 621.6 0 67 167 58 621.6 0 58 168 47 621.6 0 47 169 62 621.6 0 62 170 54 621.6 0 54 171 55 621.6 0 55 172 53 621.6 0 53 173 45 621.6 0 45 174 42 621.6 0 42 175 54 621.6 0 54 176 56 621.6 0 56 177 51 621.6 0 51 178 53 621.6 0 53 179 58 621.6 0 58 180 56 621.6 0 56 181 149 621.6 0 149 182 107 621.6 0 107 183 291 621.6 0 291 184 65 621.6 0 65 185 65 621.6 0 65 186 71 621.6 0 71 187 57 621.6 0 57 188 161 621.6 0 161 189 89 621.6 0 89 190 93 621.6 0 93 191 96 621.6 0 96 192 79 621.6 0 79 193 69 621.6 0 69 194 129 621.6 0 129 195 271 621.6 0 271 196 111 621.6 0 111 197 92 621.6 0 92 198 710 621.6 0 710 199 79 621.6 0 79 200 51 621.6 0 51 201 50 621.6 0 50 202 62 621.6 0 62 203 85 621.6 0 85 204 84 621.6 0 84 205 139 621.6 0 139 206 140 621.6 0 140 207 352 621.6 0 352 208 935 621.6 0 935 209 156 621.6 0 156 210 294 621.6 0 294 211 180 621.6 0 180 212 225 621.6 0 225 213 931 621.6 0 931 214 547 621.6 0 547 215 450 621.6 0 450 216 116 621.6 0 116 217 168 621.6 0 168 218 281 621.6 0 281 219 330 621.6 0 330 220 577 621.6 0 577 221 856 621.6 0 856 222 892 621.6 0 892 223 3523 621.6 0 3523 224 6189 621.6 0 6189 225 49053 621.6 0 49053 226 64564 621.6 0 64564 227 44635 621.6 0 44635 228 231 621.6 0 231 229 87 621.6 0 87 230 76 621.6 0 76 231 93 621.6 0 93 232 81 621.6 0 81 233 65 621.6 0 65 234 59 621.6 0 59 235 73 621.6 0 73 236 74 621.6 0 74 237 69 621.6 0 69 238 69 621.6 0 69 239 56 621.6 0 56 240 66 621.6 0 66 241 52 621.6 0 52 242 83 621.6 0 83 243 101 621.6 0 101 244 110 621.6 0 110 245 105 621.6 0 105 246 68 621.6 0 68 247 56 621.6 0 56 248 45 621.6 0 45 249 49 621.6 0 49 250 45 621.6 0 45 251 51 621.6 0 51 252 46 621.6 0 46 253 68 621.6 0 68 254 78 621.6 0 78 255 72 621.6 0 72 256 81 621.6 0 81 257 95 621.6 0 95 258 82 621.6 0 82 259 60 621.6 0 60 260 53 621.6 0 53 261 56 621.6 0 56 262 104 621.6 0 104 263 92 621.6 0 92 264 89 621.6 0 89 265 82 621.6 0 82 266 82 621.6 0 82 267 76 621.6 0 76 268 80 621.6 0 80 269 70 621.6 0 70 270 48 621.6 0 48 271 47 621.6 0 47 272 46 621.6 0 46 273 57 621.6 0 57 274 53 621.6 0 53 275 50 621.6 0 50 276 66 621.6 0 66 277 54 621.6 0 54 278 47 621.6 0 47 279 34 621.6 0 34 280 59 621.6 0 59 281 60 621.6 0 60 282 63 621.6 0 63 283 62 621.6 0 62 284 49 621.6 0 49 285 45 621.6 0 45 286 58 621.6 0 58 287 49 621.6 0 49 288 51 621.6 0 51 289 50 621.6 0 50 290 43 621.6 0 43 291 41 621.6 0 41 292 46 621.6 0 46 293 64 621.6 0 64 294 57 621.6 0 57 295 51 621.6 0 51 296 47 621.6 0 47 297 48 621.6 0 48 298 63 621.6 0 63 299 51 621.6 0 51 300 53 621.6 0 53 301 44 621.6 0 44 Finished in 6.10 s (36 us/read; 1.66 M reads/minute). === Summary === Total reads processed: 168,971 Reads with adapters: 8,099 (4.8%) Reads that were too short: 56 (0.0%) Reads written (passing filters): 8,043 (4.8%) Total basepairs processed: 31,446,269 bp Total written (filtered): 2,113,440 bp (6.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 8099 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 12 0.0 3 0 11 1 17 1211 0.0 3 1144 64 3 18 6145 0.0 3 6037 104 3 1 19 10 0.0 3 0 9 0 1 20 5 0.0 3 1 0 1 3 21 2 0.0 3 0 0 0 2 23 1 0.0 3 0 0 0 1 29 5 0.0 3 0 0 0 5 40 1 0.0 3 0 0 0 1 41 2 0.0 3 0 0 0 2 42 420 0.0 3 0 0 0 420 45 55 0.0 3 0 0 1 54 65 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 68 170 0.0 3 0 0 0 170 69 1 0.0 3 0 0 0 1 79 1 0.0 3 1 85 1 0.0 3 0 0 0 1 93 8 0.0 3 0 0 0 8 102 8 0.0 3 0 0 0 8 105 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 143 4 0.0 3 0 0 0 4 144 17 0.0 3 0 0 0 17 145 2 0.0 3 0 0 0 2 153 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 157 2 0.0 3 0 0 0 2 164 1 0.0 3 0 0 0 1 167 1 0.0 3 0 1 182 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 230 1 0.0 3 1 281 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.27 s (34 us/read; 1.79 M reads/minute). === Summary === Total reads processed: 8,043 Reads with adapters: 18 (0.2%) Reads written (passing filters): 8,043 (100.0%) Total basepairs processed: 2,113,440 bp Total written (filtered): 2,113,372 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 18 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 27.8% C: 5.6% G: 61.1% T: 5.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 4 125.7 0 4 4 14 31.4 0 14