This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACCTAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 70.82 s (23 us/read; 2.62 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 93,370 (3.0%) Reads that were too short: 27,417 (0.9%) Reads written (passing filters): 65,953 (2.1%) Total basepairs processed: 931,490,553 bp Total written (filtered): 14,650,565 bp (1.6%) === Adapter 1 === Sequence: ACCTAT; Type: regular 5'; Length: 6; Trimmed: 93370 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 282 755.5 0 282 7 8862 755.5 0 8862 8 80 755.5 0 80 9 175 755.5 0 175 10 143 755.5 0 143 11 157 755.5 0 157 12 344 755.5 0 344 13 11564 755.5 0 11564 14 7498 755.5 0 7498 15 23 755.5 0 23 16 7 755.5 0 7 17 2 755.5 0 2 18 6 755.5 0 6 19 5 755.5 0 5 20 10 755.5 0 10 21 2 755.5 0 2 22 2 755.5 0 2 23 2 755.5 0 2 24 7 755.5 0 7 25 8 755.5 0 8 26 5 755.5 0 5 27 5 755.5 0 5 28 4 755.5 0 4 29 1 755.5 0 1 30 6 755.5 0 6 31 4 755.5 0 4 32 8 755.5 0 8 33 6 755.5 0 6 34 1 755.5 0 1 35 7 755.5 0 7 36 6 755.5 0 6 37 7 755.5 0 7 38 10 755.5 0 10 39 15 755.5 0 15 40 2948 755.5 0 2948 41 15 755.5 0 15 42 19 755.5 0 19 43 37 755.5 0 37 44 21 755.5 0 21 45 23 755.5 0 23 46 12 755.5 0 12 47 9 755.5 0 9 48 8 755.5 0 8 49 8 755.5 0 8 50 3 755.5 0 3 51 5 755.5 0 5 52 4 755.5 0 4 53 4 755.5 0 4 54 11 755.5 0 11 56 6 755.5 0 6 57 8 755.5 0 8 58 4 755.5 0 4 59 1 755.5 0 1 60 6 755.5 0 6 61 5 755.5 0 5 62 5 755.5 0 5 63 4 755.5 0 4 64 5 755.5 0 5 65 9 755.5 0 9 66 6 755.5 0 6 67 2 755.5 0 2 68 5 755.5 0 5 69 8 755.5 0 8 70 4 755.5 0 4 71 4 755.5 0 4 72 2 755.5 0 2 73 12 755.5 0 12 74 4 755.5 0 4 75 4 755.5 0 4 76 9 755.5 0 9 77 11 755.5 0 11 78 23 755.5 0 23 79 39 755.5 0 39 80 39 755.5 0 39 81 40 755.5 0 40 82 35 755.5 0 35 83 87 755.5 0 87 84 37 755.5 0 37 85 20 755.5 0 20 86 49 755.5 0 49 87 141 755.5 0 141 88 10 755.5 0 10 89 104 755.5 0 104 90 232 755.5 0 232 91 469 755.5 0 469 92 20 755.5 0 20 93 140 755.5 0 140 94 196 755.5 0 196 95 380 755.5 0 380 96 401 755.5 0 401 97 207 755.5 0 207 98 70 755.5 0 70 99 53 755.5 0 53 100 100 755.5 0 100 101 85 755.5 0 85 102 44 755.5 0 44 103 150 755.5 0 150 104 163 755.5 0 163 105 133 755.5 0 133 106 2077 755.5 0 2077 107 455 755.5 0 455 108 185 755.5 0 185 109 126 755.5 0 126 110 400 755.5 0 400 111 274 755.5 0 274 112 187 755.5 0 187 113 292 755.5 0 292 114 55 755.5 0 55 115 99 755.5 0 99 116 55 755.5 0 55 117 52 755.5 0 52 118 77 755.5 0 77 119 142 755.5 0 142 120 504 755.5 0 504 121 248 755.5 0 248 122 365 755.5 0 365 123 92 755.5 0 92 124 117 755.5 0 117 125 119 755.5 0 119 126 232 755.5 0 232 127 1938 755.5 0 1938 128 1179 755.5 0 1179 129 559 755.5 0 559 130 130 755.5 0 130 131 77 755.5 0 77 132 139 755.5 0 139 133 168 755.5 0 168 134 294 755.5 0 294 135 593 755.5 0 593 136 454 755.5 0 454 137 292 755.5 0 292 138 265 755.5 0 265 139 996 755.5 0 996 140 687 755.5 0 687 141 493 755.5 0 493 142 506 755.5 0 506 143 123 755.5 0 123 144 92 755.5 0 92 145 271 755.5 0 271 146 822 755.5 0 822 147 185 755.5 0 185 148 230 755.5 0 230 149 85 755.5 0 85 150 159 755.5 0 159 151 977 755.5 0 977 152 333 755.5 0 333 153 753 755.5 0 753 154 304 755.5 0 304 155 278 755.5 0 278 156 142 755.5 0 142 157 125 755.5 0 125 158 333 755.5 0 333 159 246 755.5 0 246 160 279 755.5 0 279 161 780 755.5 0 780 162 170 755.5 0 170 163 228 755.5 0 228 164 160 755.5 0 160 165 266 755.5 0 266 166 306 755.5 0 306 167 236 755.5 0 236 168 128 755.5 0 128 169 77 755.5 0 77 170 152 755.5 0 152 171 134 755.5 0 134 172 144 755.5 0 144 173 214 755.5 0 214 174 618 755.5 0 618 175 172 755.5 0 172 176 146 755.5 0 146 177 103 755.5 0 103 178 65 755.5 0 65 179 204 755.5 0 204 180 186 755.5 0 186 181 356 755.5 0 356 182 269 755.5 0 269 183 307 755.5 0 307 184 204 755.5 0 204 185 276 755.5 0 276 186 235 755.5 0 235 187 201 755.5 0 201 188 103 755.5 0 103 189 94 755.5 0 94 190 199 755.5 0 199 191 317 755.5 0 317 192 603 755.5 0 603 193 170 755.5 0 170 194 275 755.5 0 275 195 84 755.5 0 84 196 57 755.5 0 57 197 111 755.5 0 111 198 373 755.5 0 373 199 293 755.5 0 293 200 160 755.5 0 160 201 135 755.5 0 135 202 177 755.5 0 177 203 305 755.5 0 305 204 322 755.5 0 322 205 122 755.5 0 122 206 288 755.5 0 288 207 306 755.5 0 306 208 297 755.5 0 297 209 233 755.5 0 233 210 138 755.5 0 138 211 151 755.5 0 151 212 151 755.5 0 151 213 187 755.5 0 187 214 309 755.5 0 309 215 279 755.5 0 279 216 209 755.5 0 209 217 202 755.5 0 202 218 197 755.5 0 197 219 212 755.5 0 212 220 426 755.5 0 426 221 198 755.5 0 198 222 103 755.5 0 103 223 144 755.5 0 144 224 332 755.5 0 332 225 234 755.5 0 234 226 248 755.5 0 248 227 242 755.5 0 242 228 242 755.5 0 242 229 177 755.5 0 177 230 129 755.5 0 129 231 169 755.5 0 169 232 150 755.5 0 150 233 205 755.5 0 205 234 259 755.5 0 259 235 376 755.5 0 376 236 347 755.5 0 347 237 455 755.5 0 455 238 118 755.5 0 118 239 107 755.5 0 107 240 282 755.5 0 282 241 357 755.5 0 357 242 285 755.5 0 285 243 301 755.5 0 301 244 273 755.5 0 273 245 301 755.5 0 301 246 183 755.5 0 183 247 186 755.5 0 186 248 102 755.5 0 102 249 85 755.5 0 85 250 42 755.5 0 42 251 213 755.5 0 213 252 273 755.5 0 273 253 445 755.5 0 445 254 238 755.5 0 238 255 252 755.5 0 252 256 367 755.5 0 367 257 292 755.5 0 292 258 221 755.5 0 221 259 111 755.5 0 111 260 154 755.5 0 154 261 273 755.5 0 273 262 288 755.5 0 288 263 308 755.5 0 308 264 235 755.5 0 235 265 205 755.5 0 205 266 272 755.5 0 272 267 1167 755.5 0 1167 268 587 755.5 0 587 269 461 755.5 0 461 270 99 755.5 0 99 271 158 755.5 0 158 272 167 755.5 0 167 273 145 755.5 0 145 274 259 755.5 0 259 275 210 755.5 0 210 276 203 755.5 0 203 277 481 755.5 0 481 278 384 755.5 0 384 279 564 755.5 0 564 280 1129 755.5 0 1129 281 865 755.5 0 865 282 552 755.5 0 552 283 429 755.5 0 429 284 328 755.5 0 328 285 240 755.5 0 240 286 255 755.5 0 255 287 351 755.5 0 351 288 517 755.5 0 517 289 285 755.5 0 285 290 143 755.5 0 143 291 130 755.5 0 130 292 169 755.5 0 169 293 381 755.5 0 381 294 311 755.5 0 311 295 251 755.5 0 251 296 245 755.5 0 245 297 108 755.5 0 108 298 68 755.5 0 68 299 104 755.5 0 104 300 173 755.5 0 173 301 208 755.5 0 208 Finished in 2.15 s (33 us/read; 1.84 M reads/minute). === Summary === Total reads processed: 65,953 Reads with adapters: 8,918 (13.5%) Reads that were too short: 22 (0.0%) Reads written (passing filters): 8,896 (13.5%) Total basepairs processed: 14,650,565 bp Total written (filtered): 2,454,588 bp (16.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 8918 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 23 0.0 3 0 20 2 1 17 8766 0.0 3 8681 82 3 18 18 0.0 3 1 15 1 1 19 19 0.0 3 0 0 18 1 20 7 0.0 3 0 0 0 7 32 3 0.0 3 0 0 0 3 34 3 0.0 3 0 0 1 2 35 1 0.0 3 0 0 0 1 36 1 0.0 3 1 48 2 0.0 3 0 0 0 2 53 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 1 58 2 0.0 3 0 0 0 2 67 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 88 3 0.0 3 0 0 0 3 93 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 1 110 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 159 2 0.0 3 0 0 0 2 160 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 178 6 0.0 3 0 0 0 6 179 13 0.0 3 0 0 0 13 180 3 0.0 3 0 0 1 2 181 3 0.0 3 0 0 0 3 182 14 0.0 3 0 0 0 14 188 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 271 3 0.0 3 0 0 0 3 272 1 0.0 3 0 0 0 1 274 1 0.0 3 0 0 0 1 275 6 0.0 3 0 0 0 6 276 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.28 s (31 us/read; 1.91 M reads/minute). === Summary === Total reads processed: 8,896 Reads with adapters: 621 (7.0%) Reads written (passing filters): 8,896 (100.0%) Total basepairs processed: 2,454,588 bp Total written (filtered): 2,452,677 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 621 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 53.8% C: 20.1% G: 13.7% T: 12.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 582 139.0 0 582 4 33 34.8 0 33 5 5 8.7 0 5 8 1 0.1 0 1