This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCACT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 70.44 s (23 us/read; 2.64 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 195,193 (6.3%) Reads that were too short: 42,415 (1.4%) Reads written (passing filters): 152,778 (4.9%) Total basepairs processed: 931,490,553 bp Total written (filtered): 28,922,298 bp (3.1%) === Adapter 1 === Sequence: AGCACT; Type: regular 5'; Length: 6; Trimmed: 195193 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 199 755.5 0 199 7 10049 755.5 0 10049 8 10035 755.5 0 10035 9 19 755.5 0 19 10 11 755.5 0 11 11 17 755.5 0 17 12 82 755.5 0 82 13 34 755.5 0 34 14 234 755.5 0 234 15 7 755.5 0 7 16 7 755.5 0 7 17 4 755.5 0 4 18 10 755.5 0 10 19 3 755.5 0 3 20 11 755.5 0 11 21 11 755.5 0 11 22 8 755.5 0 8 23 14 755.5 0 14 24 10 755.5 0 10 25 39 755.5 0 39 26 53 755.5 0 53 27 145 755.5 0 145 28 10 755.5 0 10 29 3 755.5 0 3 30 12 755.5 0 12 31 9 755.5 0 9 32 9 755.5 0 9 33 9 755.5 0 9 34 8 755.5 0 8 35 12 755.5 0 12 36 20 755.5 0 20 37 11 755.5 0 11 38 12 755.5 0 12 39 10 755.5 0 10 40 18 755.5 0 18 41 13 755.5 0 13 42 5 755.5 0 5 43 20 755.5 0 20 44 12 755.5 0 12 45 10 755.5 0 10 46 8 755.5 0 8 47 11 755.5 0 11 48 12 755.5 0 12 49 13 755.5 0 13 50 15 755.5 0 15 51 14 755.5 0 14 52 1 755.5 0 1 53 7 755.5 0 7 54 16 755.5 0 16 55 14 755.5 0 14 56 42 755.5 0 42 57 13 755.5 0 13 58 2 755.5 0 2 59 14 755.5 0 14 60 11 755.5 0 11 61 11 755.5 0 11 62 25 755.5 0 25 63 13 755.5 0 13 64 13 755.5 0 13 65 53 755.5 0 53 66 37 755.5 0 37 67 39 755.5 0 39 68 10 755.5 0 10 69 17 755.5 0 17 70 18 755.5 0 18 71 10 755.5 0 10 72 16 755.5 0 16 73 37 755.5 0 37 74 33 755.5 0 33 75 25 755.5 0 25 76 12 755.5 0 12 77 58 755.5 0 58 78 55 755.5 0 55 79 86 755.5 0 86 80 87 755.5 0 87 81 155 755.5 0 155 82 57 755.5 0 57 83 71 755.5 0 71 84 114 755.5 0 114 85 32 755.5 0 32 86 120 755.5 0 120 87 289 755.5 0 289 88 242 755.5 0 242 89 193 755.5 0 193 90 505 755.5 0 505 91 699 755.5 0 699 92 179 755.5 0 179 93 323 755.5 0 323 94 484 755.5 0 484 95 266 755.5 0 266 96 246 755.5 0 246 97 334 755.5 0 334 98 270 755.5 0 270 99 372 755.5 0 372 100 341 755.5 0 341 101 595 755.5 0 595 102 614 755.5 0 614 103 736 755.5 0 736 104 12429 755.5 0 12429 105 17088 755.5 0 17088 106 36739 755.5 0 36739 107 197 755.5 0 197 108 38 755.5 0 38 109 18 755.5 0 18 110 61 755.5 0 61 111 93 755.5 0 93 112 135 755.5 0 135 113 115 755.5 0 115 114 95 755.5 0 95 115 48 755.5 0 48 116 56 755.5 0 56 117 98 755.5 0 98 118 63 755.5 0 63 119 278 755.5 0 278 120 328 755.5 0 328 121 134 755.5 0 134 122 128 755.5 0 128 123 70 755.5 0 70 124 121 755.5 0 121 125 124 755.5 0 124 126 147 755.5 0 147 127 114 755.5 0 114 128 152 755.5 0 152 129 213 755.5 0 213 130 103 755.5 0 103 131 77 755.5 0 77 132 219 755.5 0 219 133 127 755.5 0 127 134 120 755.5 0 120 135 112 755.5 0 112 136 87 755.5 0 87 137 118 755.5 0 118 138 252 755.5 0 252 139 216 755.5 0 216 140 142 755.5 0 142 141 117 755.5 0 117 142 299 755.5 0 299 143 110 755.5 0 110 144 173 755.5 0 173 145 227 755.5 0 227 146 202 755.5 0 202 147 179 755.5 0 179 148 136 755.5 0 136 149 99 755.5 0 99 150 91 755.5 0 91 151 166 755.5 0 166 152 212 755.5 0 212 153 330 755.5 0 330 154 345 755.5 0 345 155 373 755.5 0 373 156 291 755.5 0 291 157 675 755.5 0 675 158 1338 755.5 0 1338 159 26071 755.5 0 26071 160 6226 755.5 0 6226 161 6385 755.5 0 6385 162 198 755.5 0 198 163 201 755.5 0 201 164 179 755.5 0 179 165 129 755.5 0 129 166 124 755.5 0 124 167 266 755.5 0 266 168 222 755.5 0 222 169 199 755.5 0 199 170 118 755.5 0 118 171 209 755.5 0 209 172 226 755.5 0 226 173 166 755.5 0 166 174 150 755.5 0 150 175 71 755.5 0 71 176 72 755.5 0 72 177 96 755.5 0 96 178 141 755.5 0 141 179 179 755.5 0 179 180 242 755.5 0 242 181 292 755.5 0 292 182 220 755.5 0 220 183 194 755.5 0 194 184 222 755.5 0 222 185 224 755.5 0 224 186 129 755.5 0 129 187 356 755.5 0 356 188 226 755.5 0 226 189 168 755.5 0 168 190 210 755.5 0 210 191 121 755.5 0 121 192 158 755.5 0 158 193 68 755.5 0 68 194 63 755.5 0 63 195 163 755.5 0 163 196 171 755.5 0 171 197 101 755.5 0 101 198 120 755.5 0 120 199 171 755.5 0 171 200 1526 755.5 0 1526 201 697 755.5 0 697 202 346 755.5 0 346 203 133 755.5 0 133 204 235 755.5 0 235 205 222 755.5 0 222 206 175 755.5 0 175 207 216 755.5 0 216 208 276 755.5 0 276 209 133 755.5 0 133 210 198 755.5 0 198 211 138 755.5 0 138 212 162 755.5 0 162 213 152 755.5 0 152 214 143 755.5 0 143 215 254 755.5 0 254 216 237 755.5 0 237 217 231 755.5 0 231 218 184 755.5 0 184 219 216 755.5 0 216 220 280 755.5 0 280 221 601 755.5 0 601 222 180 755.5 0 180 223 149 755.5 0 149 224 335 755.5 0 335 225 248 755.5 0 248 226 216 755.5 0 216 227 187 755.5 0 187 228 462 755.5 0 462 229 239 755.5 0 239 230 171 755.5 0 171 231 134 755.5 0 134 232 190 755.5 0 190 233 126 755.5 0 126 234 91 755.5 0 91 235 196 755.5 0 196 236 253 755.5 0 253 237 135 755.5 0 135 238 359 755.5 0 359 239 605 755.5 0 605 240 10284 755.5 0 10284 241 5085 755.5 0 5085 242 1050 755.5 0 1050 243 280 755.5 0 280 244 263 755.5 0 263 245 287 755.5 0 287 246 174 755.5 0 174 247 171 755.5 0 171 248 324 755.5 0 324 249 208 755.5 0 208 250 266 755.5 0 266 251 214 755.5 0 214 252 251 755.5 0 251 253 396 755.5 0 396 254 855 755.5 0 855 255 684 755.5 0 684 256 227 755.5 0 227 257 156 755.5 0 156 258 124 755.5 0 124 259 84 755.5 0 84 260 84 755.5 0 84 261 76 755.5 0 76 262 105 755.5 0 105 263 195 755.5 0 195 264 337 755.5 0 337 265 269 755.5 0 269 266 201 755.5 0 201 267 157 755.5 0 157 268 232 755.5 0 232 269 212 755.5 0 212 270 193 755.5 0 193 271 252 755.5 0 252 272 194 755.5 0 194 273 189 755.5 0 189 274 219 755.5 0 219 275 245 755.5 0 245 276 215 755.5 0 215 277 235 755.5 0 235 278 261 755.5 0 261 279 379 755.5 0 379 280 558 755.5 0 558 281 341 755.5 0 341 282 353 755.5 0 353 283 836 755.5 0 836 284 226 755.5 0 226 285 249 755.5 0 249 286 200 755.5 0 200 287 170 755.5 0 170 288 100 755.5 0 100 289 147 755.5 0 147 290 274 755.5 0 274 291 407 755.5 0 407 292 242 755.5 0 242 293 212 755.5 0 212 294 251 755.5 0 251 295 137 755.5 0 137 296 121 755.5 0 121 297 403 755.5 0 403 298 420 755.5 0 420 299 1156 755.5 0 1156 300 769 755.5 0 769 301 402 755.5 0 402 Finished in 5.29 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 152,778 Reads with adapters: 15,752 (10.3%) Reads that were too short: 32 (0.0%) Reads written (passing filters): 15,720 (10.3%) Total basepairs processed: 28,922,298 bp Total written (filtered): 3,708,452 bp (12.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 15752 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 5 0.0 3 0 0 5 19 102 0.0 3 0 87 5 6 4 20 10160 0.0 4 9838 287 26 7 2 21 22 0.0 4 3 17 1 1 22 2 0.0 4 0 0 2 23 7 0.0 4 1 0 0 4 2 30 3945 0.0 4 0 0 0 0 3945 47 1 0.0 4 0 0 0 0 1 52 1 0.0 4 0 0 0 0 1 54 175 0.0 4 0 0 0 1 174 55 17 0.0 4 0 0 0 0 17 70 1 0.0 4 0 0 0 0 1 78 1 0.0 4 0 0 0 1 98 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 125 3 0.0 4 0 0 0 0 3 126 16 0.0 4 0 0 0 0 16 127 1257 0.0 4 0 0 0 0 1257 128 3 0.0 4 0 0 0 0 3 137 1 0.0 4 0 0 0 0 1 138 2 0.0 4 0 0 0 1 1 139 1 0.0 4 0 0 0 0 1 157 1 0.0 4 0 0 0 0 1 160 1 0.0 4 0 0 0 0 1 161 1 0.0 4 0 0 0 0 1 166 1 0.0 4 0 0 0 0 1 185 5 0.0 4 0 0 0 0 5 186 1 0.0 4 0 0 0 0 1 187 1 0.0 4 0 0 0 0 1 190 1 0.0 4 0 0 0 0 1 191 4 0.0 4 0 0 0 0 4 192 3 0.0 4 0 0 0 0 3 194 4 0.0 4 0 0 0 0 4 204 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.44 s (28 us/read; 2.14 M reads/minute). === Summary === Total reads processed: 15,720 Reads with adapters: 1,295 (8.2%) Reads written (passing filters): 15,720 (100.0%) Total basepairs processed: 3,708,452 bp Total written (filtered): 3,691,354 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1295 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.8% C: 1.6% G: 92.5% T: 2.0% none/other: 0.1% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 3 245.6 0 3 4 20 61.4 0 20 5 4 15.4 0 4 10 1 0.0 1 0 1 11 1251 0.0 1 2 1249 12 4 0.0 1 0 4 258 1 0.0 1 1 259 1 0.0 1 1 260 1 0.0 1 1 264 7 0.0 1 7 272 1 0.0 1 1 273 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.