This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CATTAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 70.27 s (23 us/read; 2.64 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 56,549 (1.8%) Reads that were too short: 14,008 (0.5%) Reads written (passing filters): 42,541 (1.4%) Total basepairs processed: 931,490,553 bp Total written (filtered): 9,523,265 bp (1.0%) === Adapter 1 === Sequence: CATTAG; Type: regular 5'; Length: 6; Trimmed: 56549 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 202 755.5 0 202 7 9474 755.5 0 9474 8 31 755.5 0 31 9 8 755.5 0 8 10 11 755.5 0 11 11 7 755.5 0 7 12 7 755.5 0 7 13 12 755.5 0 12 14 16 755.5 0 16 15 14 755.5 0 14 16 6 755.5 0 6 17 23 755.5 0 23 18 16 755.5 0 16 19 14 755.5 0 14 20 24 755.5 0 24 21 62 755.5 0 62 22 22 755.5 0 22 23 27 755.5 0 27 24 33 755.5 0 33 25 46 755.5 0 46 26 25 755.5 0 25 27 56 755.5 0 56 28 117 755.5 0 117 29 60 755.5 0 60 30 36 755.5 0 36 31 59 755.5 0 59 32 110 755.5 0 110 33 150 755.5 0 150 34 6897 755.5 0 6897 35 53 755.5 0 53 36 61 755.5 0 61 37 13 755.5 0 13 38 26 755.5 0 26 39 30 755.5 0 30 40 48 755.5 0 48 41 57 755.5 0 57 42 72 755.5 0 72 43 2207 755.5 0 2207 44 584 755.5 0 584 45 12 755.5 0 12 46 9 755.5 0 9 47 12 755.5 0 12 48 12 755.5 0 12 49 6 755.5 0 6 50 16 755.5 0 16 51 12 755.5 0 12 52 20 755.5 0 20 53 10 755.5 0 10 54 17 755.5 0 17 55 29 755.5 0 29 56 17 755.5 0 17 57 38 755.5 0 38 58 168 755.5 0 168 59 64 755.5 0 64 60 34 755.5 0 34 61 20 755.5 0 20 62 19 755.5 0 19 63 12 755.5 0 12 64 14 755.5 0 14 65 17 755.5 0 17 66 23 755.5 0 23 67 26 755.5 0 26 68 45 755.5 0 45 69 29 755.5 0 29 70 72 755.5 0 72 71 112 755.5 0 112 72 1014 755.5 0 1014 73 471 755.5 0 471 74 804 755.5 0 804 75 15 755.5 0 15 76 8 755.5 0 8 77 20 755.5 0 20 78 13 755.5 0 13 79 12 755.5 0 12 80 36 755.5 0 36 81 35 755.5 0 35 82 20 755.5 0 20 83 28 755.5 0 28 84 32 755.5 0 32 85 25 755.5 0 25 86 39 755.5 0 39 87 51 755.5 0 51 88 74 755.5 0 74 89 543 755.5 0 543 90 804 755.5 0 804 91 59 755.5 0 59 92 484 755.5 0 484 93 77 755.5 0 77 94 54 755.5 0 54 95 101 755.5 0 101 96 96 755.5 0 96 97 184 755.5 0 184 98 19 755.5 0 19 99 32 755.5 0 32 100 53 755.5 0 53 101 21 755.5 0 21 102 13 755.5 0 13 103 28 755.5 0 28 104 29 755.5 0 29 105 15 755.5 0 15 106 57 755.5 0 57 107 28 755.5 0 28 108 25 755.5 0 25 109 180 755.5 0 180 110 132 755.5 0 132 111 30 755.5 0 30 112 43 755.5 0 43 113 112 755.5 0 112 114 256 755.5 0 256 115 41 755.5 0 41 116 38 755.5 0 38 117 25 755.5 0 25 118 44 755.5 0 44 119 23 755.5 0 23 120 15 755.5 0 15 121 58 755.5 0 58 122 63 755.5 0 63 123 48 755.5 0 48 124 47 755.5 0 47 125 41 755.5 0 41 126 41 755.5 0 41 127 64 755.5 0 64 128 89 755.5 0 89 129 60 755.5 0 60 130 144 755.5 0 144 131 102 755.5 0 102 132 56 755.5 0 56 133 118 755.5 0 118 134 163 755.5 0 163 135 141 755.5 0 141 136 152 755.5 0 152 137 142 755.5 0 142 138 92 755.5 0 92 139 93 755.5 0 93 140 87 755.5 0 87 141 143 755.5 0 143 142 171 755.5 0 171 143 2126 755.5 0 2126 144 1149 755.5 0 1149 145 4786 755.5 0 4786 146 399 755.5 0 399 147 48 755.5 0 48 148 55 755.5 0 55 149 48 755.5 0 48 150 49 755.5 0 49 151 33 755.5 0 33 152 27 755.5 0 27 153 35 755.5 0 35 154 55 755.5 0 55 155 105 755.5 0 105 156 264 755.5 0 264 157 121 755.5 0 121 158 43 755.5 0 43 159 69 755.5 0 69 160 35 755.5 0 35 161 60 755.5 0 60 162 57 755.5 0 57 163 111 755.5 0 111 164 88 755.5 0 88 165 186 755.5 0 186 166 77 755.5 0 77 167 101 755.5 0 101 168 91 755.5 0 91 169 61 755.5 0 61 170 80 755.5 0 80 171 30 755.5 0 30 172 28 755.5 0 28 173 66 755.5 0 66 174 55 755.5 0 55 175 62 755.5 0 62 176 50 755.5 0 50 177 43 755.5 0 43 178 25 755.5 0 25 179 35 755.5 0 35 180 52 755.5 0 52 181 64 755.5 0 64 182 84 755.5 0 84 183 78 755.5 0 78 184 100 755.5 0 100 185 63 755.5 0 63 186 61 755.5 0 61 187 61 755.5 0 61 188 50 755.5 0 50 189 52 755.5 0 52 190 107 755.5 0 107 191 103 755.5 0 103 192 87 755.5 0 87 193 76 755.5 0 76 194 200 755.5 0 200 195 117 755.5 0 117 196 73 755.5 0 73 197 95 755.5 0 95 198 43 755.5 0 43 199 67 755.5 0 67 200 50 755.5 0 50 201 51 755.5 0 51 202 165 755.5 0 165 203 371 755.5 0 371 204 188 755.5 0 188 205 117 755.5 0 117 206 88 755.5 0 88 207 88 755.5 0 88 208 348 755.5 0 348 209 244 755.5 0 244 210 265 755.5 0 265 211 135 755.5 0 135 212 112 755.5 0 112 213 157 755.5 0 157 214 485 755.5 0 485 215 909 755.5 0 909 216 286 755.5 0 286 217 176 755.5 0 176 218 82 755.5 0 82 219 62 755.5 0 62 220 70 755.5 0 70 221 56 755.5 0 56 222 73 755.5 0 73 223 172 755.5 0 172 224 64 755.5 0 64 225 107 755.5 0 107 226 158 755.5 0 158 227 107 755.5 0 107 228 108 755.5 0 108 229 91 755.5 0 91 230 108 755.5 0 108 231 226 755.5 0 226 232 61 755.5 0 61 233 90 755.5 0 90 234 114 755.5 0 114 235 127 755.5 0 127 236 138 755.5 0 138 237 139 755.5 0 139 238 90 755.5 0 90 239 64 755.5 0 64 240 44 755.5 0 44 241 42 755.5 0 42 242 50 755.5 0 50 243 88 755.5 0 88 244 156 755.5 0 156 245 116 755.5 0 116 246 88 755.5 0 88 247 54 755.5 0 54 248 55 755.5 0 55 249 49 755.5 0 49 250 55 755.5 0 55 251 40 755.5 0 40 252 90 755.5 0 90 253 95 755.5 0 95 254 83 755.5 0 83 255 81 755.5 0 81 256 82 755.5 0 82 257 107 755.5 0 107 258 59 755.5 0 59 259 91 755.5 0 91 260 81 755.5 0 81 261 108 755.5 0 108 262 80 755.5 0 80 263 85 755.5 0 85 264 51 755.5 0 51 265 95 755.5 0 95 266 77 755.5 0 77 267 160 755.5 0 160 268 94 755.5 0 94 269 65 755.5 0 65 270 82 755.5 0 82 271 67 755.5 0 67 272 67 755.5 0 67 273 85 755.5 0 85 274 86 755.5 0 86 275 115 755.5 0 115 276 102 755.5 0 102 277 64 755.5 0 64 278 93 755.5 0 93 279 78 755.5 0 78 280 1406 755.5 0 1406 281 304 755.5 0 304 282 515 755.5 0 515 283 160 755.5 0 160 284 75 755.5 0 75 285 129 755.5 0 129 286 105 755.5 0 105 287 124 755.5 0 124 288 120 755.5 0 120 289 79 755.5 0 79 290 71 755.5 0 71 291 56 755.5 0 56 292 43 755.5 0 43 293 134 755.5 0 134 294 108 755.5 0 108 295 92 755.5 0 92 296 172 755.5 0 172 297 203 755.5 0 203 298 139 755.5 0 139 299 145 755.5 0 145 300 102 755.5 0 102 301 25 755.5 0 25 Finished in 1.42 s (33 us/read; 1.80 M reads/minute). === Summary === Total reads processed: 42,541 Reads with adapters: 9,670 (22.7%) Reads that were too short: 9 (0.0%) Reads written (passing filters): 9,661 (22.7%) Total basepairs processed: 9,523,265 bp Total written (filtered): 2,669,970 bp (28.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 9670 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 3 0.0 3 0 0 2 1 16 49 0.0 3 0 49 17 9526 0.0 3 9347 174 4 1 18 20 0.0 3 1 16 3 19 20 0.0 3 17 0 3 20 3 0.0 3 0 0 0 3 23 1 0.0 3 1 43 1 0.0 3 1 47 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 49 1 0.0 3 1 74 1 0.0 3 0 0 0 1 79 2 0.0 3 0 0 0 2 81 1 0.0 3 0 0 0 1 100 28 0.0 3 0 0 0 28 101 1 0.0 3 0 0 0 1 111 2 0.0 3 0 0 0 2 128 1 0.0 3 0 0 0 1 150 6 0.0 3 0 0 0 6 160 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.30 s (31 us/read; 1.93 M reads/minute). === Summary === Total reads processed: 9,661 Reads with adapters: 23 (0.2%) Reads written (passing filters): 9,661 (100.0%) Total basepairs processed: 2,669,970 bp Total written (filtered): 2,669,896 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 23 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 43.5% C: 34.8% G: 8.7% T: 13.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 21 151.0 0 21 5 1 9.4 0 1 6 1 2.4 0 1