This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TAATGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 95.72 s (30 us/read; 2.00 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 185,495 (5.8%) Reads that were too short: 41,110 (1.3%) Reads written (passing filters): 144,385 (4.5%) Total basepairs processed: 961,105,341 bp Total written (filtered): 27,578,927 bp (2.9%) === Adapter 1 === Sequence: TAATGA; Type: regular 5'; Length: 6; Trimmed: 185495 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 377 779.6 0 377 7 21408 779.6 0 21408 8 45 779.6 0 45 9 3 779.6 0 3 10 1 779.6 0 1 11 8 779.6 0 8 12 6 779.6 0 6 13 5 779.6 0 5 14 5 779.6 0 5 15 10 779.6 0 10 16 3 779.6 0 3 17 11 779.6 0 11 18 5 779.6 0 5 19 10 779.6 0 10 20 9 779.6 0 9 21 8 779.6 0 8 22 14 779.6 0 14 23 11 779.6 0 11 24 11 779.6 0 11 25 9 779.6 0 9 26 7 779.6 0 7 27 18 779.6 0 18 28 11 779.6 0 11 29 88 779.6 0 88 30 262 779.6 0 262 31 128 779.6 0 128 32 7 779.6 0 7 33 7 779.6 0 7 34 10 779.6 0 10 35 5 779.6 0 5 36 14 779.6 0 14 37 7 779.6 0 7 38 7 779.6 0 7 39 14 779.6 0 14 40 14 779.6 0 14 41 3 779.6 0 3 42 17 779.6 0 17 43 8 779.6 0 8 44 14 779.6 0 14 45 6 779.6 0 6 46 9 779.6 0 9 47 8 779.6 0 8 48 10 779.6 0 10 49 6 779.6 0 6 50 7 779.6 0 7 51 5 779.6 0 5 52 9 779.6 0 9 53 7 779.6 0 7 54 10 779.6 0 10 55 11 779.6 0 11 56 17 779.6 0 17 57 13 779.6 0 13 58 6 779.6 0 6 59 16 779.6 0 16 60 15 779.6 0 15 61 10 779.6 0 10 62 9 779.6 0 9 63 13 779.6 0 13 64 13 779.6 0 13 65 22 779.6 0 22 66 22 779.6 0 22 67 35 779.6 0 35 68 43 779.6 0 43 69 54 779.6 0 54 70 803 779.6 0 803 71 544 779.6 0 544 72 1509 779.6 0 1509 73 35 779.6 0 35 74 13 779.6 0 13 75 20 779.6 0 20 76 9 779.6 0 9 77 14 779.6 0 14 78 38 779.6 0 38 79 120 779.6 0 120 80 90 779.6 0 90 81 161 779.6 0 161 82 289 779.6 0 289 83 330 779.6 0 330 84 42 779.6 0 42 85 102 779.6 0 102 86 32 779.6 0 32 87 11 779.6 0 11 88 53 779.6 0 53 89 67 779.6 0 67 90 9 779.6 0 9 91 6 779.6 0 6 92 152 779.6 0 152 93 175 779.6 0 175 94 141 779.6 0 141 95 116 779.6 0 116 96 56 779.6 0 56 97 27 779.6 0 27 98 259 779.6 0 259 99 649 779.6 0 649 100 275 779.6 0 275 101 54 779.6 0 54 102 128 779.6 0 128 103 45 779.6 0 45 104 75 779.6 0 75 105 191 779.6 0 191 106 99 779.6 0 99 107 158 779.6 0 158 108 218 779.6 0 218 109 504 779.6 0 504 110 408 779.6 0 408 111 784 779.6 0 784 112 212 779.6 0 212 113 261 779.6 0 261 114 538 779.6 0 538 115 366 779.6 0 366 116 298 779.6 0 298 117 653 779.6 0 653 118 459 779.6 0 459 119 406 779.6 0 406 120 463 779.6 0 463 121 473 779.6 0 473 122 616 779.6 0 616 123 1181 779.6 0 1181 124 16308 779.6 0 16308 125 18131 779.6 0 18131 126 45603 779.6 0 45603 127 1184 779.6 0 1184 128 109 779.6 0 109 129 179 779.6 0 179 130 227 779.6 0 227 131 203 779.6 0 203 132 112 779.6 0 112 133 145 779.6 0 145 134 167 779.6 0 167 135 241 779.6 0 241 136 279 779.6 0 279 137 187 779.6 0 187 138 189 779.6 0 189 139 203 779.6 0 203 140 468 779.6 0 468 141 173 779.6 0 173 142 263 779.6 0 263 143 203 779.6 0 203 144 352 779.6 0 352 145 212 779.6 0 212 146 160 779.6 0 160 147 151 779.6 0 151 148 330 779.6 0 330 149 2497 779.6 0 2497 150 2584 779.6 0 2584 151 6643 779.6 0 6643 152 204 779.6 0 204 153 142 779.6 0 142 154 93 779.6 0 93 155 118 779.6 0 118 156 408 779.6 0 408 157 206 779.6 0 206 158 209 779.6 0 209 159 151 779.6 0 151 160 104 779.6 0 104 161 144 779.6 0 144 162 153 779.6 0 153 163 214 779.6 0 214 164 303 779.6 0 303 165 662 779.6 0 662 166 167 779.6 0 167 167 180 779.6 0 180 168 219 779.6 0 219 169 291 779.6 0 291 170 97 779.6 0 97 171 101 779.6 0 101 172 121 779.6 0 121 173 179 779.6 0 179 174 57 779.6 0 57 175 58 779.6 0 58 176 82 779.6 0 82 177 97 779.6 0 97 178 172 779.6 0 172 179 126 779.6 0 126 180 137 779.6 0 137 181 253 779.6 0 253 182 352 779.6 0 352 183 356 779.6 0 356 184 228 779.6 0 228 185 218 779.6 0 218 186 185 779.6 0 185 187 71 779.6 0 71 188 144 779.6 0 144 189 197 779.6 0 197 190 198 779.6 0 198 191 173 779.6 0 173 192 140 779.6 0 140 193 146 779.6 0 146 194 107 779.6 0 107 195 105 779.6 0 105 196 246 779.6 0 246 197 438 779.6 0 438 198 182 779.6 0 182 199 76 779.6 0 76 200 47 779.6 0 47 201 112 779.6 0 112 202 260 779.6 0 260 203 209 779.6 0 209 204 173 779.6 0 173 205 265 779.6 0 265 206 188 779.6 0 188 207 156 779.6 0 156 208 229 779.6 0 229 209 238 779.6 0 238 210 220 779.6 0 220 211 217 779.6 0 217 212 190 779.6 0 190 213 269 779.6 0 269 214 204 779.6 0 204 215 189 779.6 0 189 216 290 779.6 0 290 217 268 779.6 0 268 218 276 779.6 0 276 219 186 779.6 0 186 220 157 779.6 0 157 221 271 779.6 0 271 222 501 779.6 0 501 223 1555 779.6 0 1555 224 926 779.6 0 926 225 7114 779.6 0 7114 226 4490 779.6 0 4490 227 5589 779.6 0 5589 228 218 779.6 0 218 229 208 779.6 0 208 230 201 779.6 0 201 231 140 779.6 0 140 232 128 779.6 0 128 233 248 779.6 0 248 234 475 779.6 0 475 235 270 779.6 0 270 236 423 779.6 0 423 237 344 779.6 0 344 238 195 779.6 0 195 239 132 779.6 0 132 240 108 779.6 0 108 241 152 779.6 0 152 242 153 779.6 0 153 243 194 779.6 0 194 244 177 779.6 0 177 245 216 779.6 0 216 246 209 779.6 0 209 247 136 779.6 0 136 248 91 779.6 0 91 249 80 779.6 0 80 250 188 779.6 0 188 251 249 779.6 0 249 252 141 779.6 0 141 253 101 779.6 0 101 254 193 779.6 0 193 255 210 779.6 0 210 256 250 779.6 0 250 257 174 779.6 0 174 258 124 779.6 0 124 259 147 779.6 0 147 260 216 779.6 0 216 261 266 779.6 0 266 262 315 779.6 0 315 263 191 779.6 0 191 264 167 779.6 0 167 265 209 779.6 0 209 266 308 779.6 0 308 267 259 779.6 0 259 268 192 779.6 0 192 269 125 779.6 0 125 270 283 779.6 0 283 271 317 779.6 0 317 272 290 779.6 0 290 273 390 779.6 0 390 274 312 779.6 0 312 275 136 779.6 0 136 276 167 779.6 0 167 277 236 779.6 0 236 278 236 779.6 0 236 279 247 779.6 0 247 280 110 779.6 0 110 281 123 779.6 0 123 282 234 779.6 0 234 283 231 779.6 0 231 284 196 779.6 0 196 285 251 779.6 0 251 286 174 779.6 0 174 287 154 779.6 0 154 288 127 779.6 0 127 289 156 779.6 0 156 290 224 779.6 0 224 291 258 779.6 0 258 292 303 779.6 0 303 293 194 779.6 0 194 294 130 779.6 0 130 295 267 779.6 0 267 296 557 779.6 0 557 297 387 779.6 0 387 298 310 779.6 0 310 299 426 779.6 0 426 300 297 779.6 0 297 301 234 779.6 0 234 Finished in 5.55 s (38 us/read; 1.56 M reads/minute). === Summary === Total reads processed: 144,385 Reads with adapters: 22,022 (15.3%) Reads that were too short: 10 (0.0%) Reads written (passing filters): 22,012 (15.2%) Total basepairs processed: 27,578,927 bp Total written (filtered): 6,038,849 bp (21.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 22022 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 5 0.0 3 0 0 4 1 16 44 0.0 3 0 35 9 17 3678 0.0 3 3476 200 2 18 17917 0.0 3 17678 234 3 2 19 55 0.0 3 4 31 18 2 20 62 0.0 3 1 0 38 23 21 20 0.0 3 0 0 0 20 24 2 0.0 3 0 0 0 2 26 4 0.0 3 0 0 0 4 29 5 0.0 3 0 0 0 5 42 36 0.0 3 0 0 0 36 43 1 0.0 3 0 0 0 1 45 30 0.0 3 0 0 1 29 48 1 0.0 3 0 0 0 1 52 2 0.0 3 0 0 0 2 61 1 0.0 3 0 0 0 1 68 139 0.0 3 0 0 0 139 69 2 0.0 3 0 0 0 2 75 1 0.0 3 0 0 0 1 85 2 0.0 3 0 0 0 2 102 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 110 2 0.0 3 0 0 0 2 113 1 0.0 3 0 0 0 1 144 6 0.0 3 0 0 1 5 165 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 200 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.91 s (41 us/read; 1.45 M reads/minute). === Summary === Total reads processed: 22,012 Reads with adapters: 1,997 (9.1%) Reads written (passing filters): 22,012 (100.0%) Total basepairs processed: 6,038,849 bp Total written (filtered): 6,032,827 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1997 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 18.0% C: 5.9% G: 74.3% T: 1.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1968 343.9 0 1968 4 27 86.0 0 27 5 2 21.5 0 2