This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGTCAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 96.89 s (30 us/read; 1.98 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 31,453 (1.0%) Reads that were too short: 14,460 (0.5%) Reads written (passing filters): 16,993 (0.5%) Total basepairs processed: 961,105,341 bp Total written (filtered): 3,556,424 bp (0.4%) === Adapter 1 === Sequence: CGTCAC; Type: regular 5'; Length: 6; Trimmed: 31453 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 583 779.6 0 583 7 57 779.6 0 57 8 3905 779.6 0 3905 9 31 779.6 0 31 10 38 779.6 0 38 11 22 779.6 0 22 12 20 779.6 0 20 13 26 779.6 0 26 14 15 779.6 0 15 15 9 779.6 0 9 16 20 779.6 0 20 17 21 779.6 0 21 18 13 779.6 0 13 19 15 779.6 0 15 20 15 779.6 0 15 21 12 779.6 0 12 22 19 779.6 0 19 23 18 779.6 0 18 24 24 779.6 0 24 25 28 779.6 0 28 26 28 779.6 0 28 27 21 779.6 0 21 28 27 779.6 0 27 29 20 779.6 0 20 30 18 779.6 0 18 31 16 779.6 0 16 32 18 779.6 0 18 33 9 779.6 0 9 34 17 779.6 0 17 35 20 779.6 0 20 36 14 779.6 0 14 37 26 779.6 0 26 38 17 779.6 0 17 39 17 779.6 0 17 40 22 779.6 0 22 41 22 779.6 0 22 42 25 779.6 0 25 43 27 779.6 0 27 44 44 779.6 0 44 45 24 779.6 0 24 46 15 779.6 0 15 47 34 779.6 0 34 48 31 779.6 0 31 49 25 779.6 0 25 50 11 779.6 0 11 51 26 779.6 0 26 52 22 779.6 0 22 53 77 779.6 0 77 54 33 779.6 0 33 55 35 779.6 0 35 56 33 779.6 0 33 57 13 779.6 0 13 58 28 779.6 0 28 59 29 779.6 0 29 60 39 779.6 0 39 61 33 779.6 0 33 62 32 779.6 0 32 63 49 779.6 0 49 64 694 779.6 0 694 65 506 779.6 0 506 66 608 779.6 0 608 67 34 779.6 0 34 68 30 779.6 0 30 69 37 779.6 0 37 70 28 779.6 0 28 71 49 779.6 0 49 72 16 779.6 0 16 73 16 779.6 0 16 74 14 779.6 0 14 75 28 779.6 0 28 76 19 779.6 0 19 77 22 779.6 0 22 78 12 779.6 0 12 79 18 779.6 0 18 80 24 779.6 0 24 81 56 779.6 0 56 82 20 779.6 0 20 83 63 779.6 0 63 84 72 779.6 0 72 85 50 779.6 0 50 86 27 779.6 0 27 87 75 779.6 0 75 88 26 779.6 0 26 89 77 779.6 0 77 90 87 779.6 0 87 91 45 779.6 0 45 92 99 779.6 0 99 93 195 779.6 0 195 94 154 779.6 0 154 95 38 779.6 0 38 96 73 779.6 0 73 97 29 779.6 0 29 98 10 779.6 0 10 99 48 779.6 0 48 100 70 779.6 0 70 101 132 779.6 0 132 102 28 779.6 0 28 103 41 779.6 0 41 104 30 779.6 0 30 105 14 779.6 0 14 106 20 779.6 0 20 107 17 779.6 0 17 108 26 779.6 0 26 109 19 779.6 0 19 110 18 779.6 0 18 111 26 779.6 0 26 112 20 779.6 0 20 113 16 779.6 0 16 114 111 779.6 0 111 115 38 779.6 0 38 116 45 779.6 0 45 117 31 779.6 0 31 118 47 779.6 0 47 119 38 779.6 0 38 120 32 779.6 0 32 121 63 779.6 0 63 122 49 779.6 0 49 123 55 779.6 0 55 124 59 779.6 0 59 125 41 779.6 0 41 126 25 779.6 0 25 127 40 779.6 0 40 128 84 779.6 0 84 129 36 779.6 0 36 130 54 779.6 0 54 131 71 779.6 0 71 132 80 779.6 0 80 133 65 779.6 0 65 134 40 779.6 0 40 135 69 779.6 0 69 136 127 779.6 0 127 137 198 779.6 0 198 138 73 779.6 0 73 139 59 779.6 0 59 140 45 779.6 0 45 141 47 779.6 0 47 142 53 779.6 0 53 143 38 779.6 0 38 144 40 779.6 0 40 145 25 779.6 0 25 146 54 779.6 0 54 147 108 779.6 0 108 148 108 779.6 0 108 149 105 779.6 0 105 150 71 779.6 0 71 151 44 779.6 0 44 152 65 779.6 0 65 153 78 779.6 0 78 154 47 779.6 0 47 155 77 779.6 0 77 156 118 779.6 0 118 157 149 779.6 0 149 158 175 779.6 0 175 159 99 779.6 0 99 160 91 779.6 0 91 161 89 779.6 0 89 162 108 779.6 0 108 163 104 779.6 0 104 164 83 779.6 0 83 165 66 779.6 0 66 166 92 779.6 0 92 167 132 779.6 0 132 168 120 779.6 0 120 169 182 779.6 0 182 170 113 779.6 0 113 171 123 779.6 0 123 172 86 779.6 0 86 173 66 779.6 0 66 174 60 779.6 0 60 175 229 779.6 0 229 176 199 779.6 0 199 177 177 779.6 0 177 178 68 779.6 0 68 179 34 779.6 0 34 180 52 779.6 0 52 181 85 779.6 0 85 182 93 779.6 0 93 183 104 779.6 0 104 184 106 779.6 0 106 185 88 779.6 0 88 186 166 779.6 0 166 187 204 779.6 0 204 188 271 779.6 0 271 189 72 779.6 0 72 190 74 779.6 0 74 191 26 779.6 0 26 192 58 779.6 0 58 193 59 779.6 0 59 194 70 779.6 0 70 195 46 779.6 0 46 196 36 779.6 0 36 197 31 779.6 0 31 198 30 779.6 0 30 199 59 779.6 0 59 200 69 779.6 0 69 201 32 779.6 0 32 202 30 779.6 0 30 203 29 779.6 0 29 204 55 779.6 0 55 205 56 779.6 0 56 206 75 779.6 0 75 207 79 779.6 0 79 208 46 779.6 0 46 209 77 779.6 0 77 210 50 779.6 0 50 211 51 779.6 0 51 212 109 779.6 0 109 213 83 779.6 0 83 214 95 779.6 0 95 215 111 779.6 0 111 216 130 779.6 0 130 217 187 779.6 0 187 218 166 779.6 0 166 219 97 779.6 0 97 220 116 779.6 0 116 221 123 779.6 0 123 222 232 779.6 0 232 223 660 779.6 0 660 224 334 779.6 0 334 225 138 779.6 0 138 226 204 779.6 0 204 227 131 779.6 0 131 228 212 779.6 0 212 229 80 779.6 0 80 230 70 779.6 0 70 231 46 779.6 0 46 232 66 779.6 0 66 233 37 779.6 0 37 234 52 779.6 0 52 235 62 779.6 0 62 236 67 779.6 0 67 237 81 779.6 0 81 238 56 779.6 0 56 239 63 779.6 0 63 240 53 779.6 0 53 241 57 779.6 0 57 242 330 779.6 0 330 243 324 779.6 0 324 244 305 779.6 0 305 245 182 779.6 0 182 246 102 779.6 0 102 247 118 779.6 0 118 248 134 779.6 0 134 249 137 779.6 0 137 250 84 779.6 0 84 251 154 779.6 0 154 252 355 779.6 0 355 253 425 779.6 0 425 254 415 779.6 0 415 255 340 779.6 0 340 256 183 779.6 0 183 257 169 779.6 0 169 258 140 779.6 0 140 259 44 779.6 0 44 260 29 779.6 0 29 261 30 779.6 0 30 262 42 779.6 0 42 263 45 779.6 0 45 264 35 779.6 0 35 265 89 779.6 0 89 266 82 779.6 0 82 267 216 779.6 0 216 268 431 779.6 0 431 269 251 779.6 0 251 270 222 779.6 0 222 271 83 779.6 0 83 272 55 779.6 0 55 273 81 779.6 0 81 274 110 779.6 0 110 275 101 779.6 0 101 276 56 779.6 0 56 277 37 779.6 0 37 278 45 779.6 0 45 279 63 779.6 0 63 280 172 779.6 0 172 281 111 779.6 0 111 282 129 779.6 0 129 283 123 779.6 0 123 284 92 779.6 0 92 285 78 779.6 0 78 286 51 779.6 0 51 287 95 779.6 0 95 288 757 779.6 0 757 289 364 779.6 0 364 290 52 779.6 0 52 291 39 779.6 0 39 292 102 779.6 0 102 293 167 779.6 0 167 294 220 779.6 0 220 295 128 779.6 0 128 296 75 779.6 0 75 297 53 779.6 0 53 298 87 779.6 0 87 299 80 779.6 0 80 300 533 779.6 0 533 301 442 779.6 0 442 Finished in 0.82 s (48 us/read; 1.24 M reads/minute). === Summary === Total reads processed: 16,993 Reads with adapters: 4,158 (24.5%) Reads that were too short: 5 (0.0%) Reads written (passing filters): 4,153 (24.4%) Total basepairs processed: 3,556,424 bp Total written (filtered): 1,133,236 bp (31.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 4158 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 4 0.0 3 0 0 3 0 1 19 31 0.0 3 0 28 0 2 1 20 3920 0.0 4 3778 119 15 2 6 21 12 0.0 4 2 9 1 22 3 0.0 4 2 1 23 178 0.0 4 169 4 1 3 1 24 1 0.0 4 0 1 26 1 0.0 4 0 0 0 1 68 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 85 1 0.0 4 0 0 0 0 1 130 2 0.0 4 0 0 0 0 2 144 1 0.0 4 0 0 0 0 1 163 1 0.0 4 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.17 s (41 us/read; 1.47 M reads/minute). === Summary === Total reads processed: 4,153 Reads with adapters: 1,035 (24.9%) Reads written (passing filters): 4,153 (100.0%) Total basepairs processed: 1,133,236 bp Total written (filtered): 1,120,791 bp (98.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1035 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.8% C: 1.7% G: 94.6% T: 1.8% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 9 2 0.0 0 0 2 10 5 0.0 1 0 5 11 1021 0.0 1 2 1019 12 1 0.0 1 0 1 151 3 0.0 1 3 152 1 0.0 1 1 260 1 0.0 1 1 269 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.