This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCTTGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 94.83 s (30 us/read; 2.02 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 54,891 (1.7%) Reads that were too short: 3,734 (0.1%) Reads written (passing filters): 51,157 (1.6%) Total basepairs processed: 961,105,341 bp Total written (filtered): 10,790,465 bp (1.1%) === Adapter 1 === Sequence: TCTTGG; Type: regular 5'; Length: 6; Trimmed: 54891 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3764 779.6 0 3764 7 2348 779.6 0 2348 8 3927 779.6 0 3927 9 5458 779.6 0 5458 10 23 779.6 0 23 11 14 779.6 0 14 12 8 779.6 0 8 13 14 779.6 0 14 14 18 779.6 0 18 15 21 779.6 0 21 16 7 779.6 0 7 17 16 779.6 0 16 18 24 779.6 0 24 19 33 779.6 0 33 20 28 779.6 0 28 21 21 779.6 0 21 22 30 779.6 0 30 23 27 779.6 0 27 24 59 779.6 0 59 25 15 779.6 0 15 26 26 779.6 0 26 27 38 779.6 0 38 28 23 779.6 0 23 29 15 779.6 0 15 30 30 779.6 0 30 31 24 779.6 0 24 32 38 779.6 0 38 33 32 779.6 0 32 34 38 779.6 0 38 35 75 779.6 0 75 36 86 779.6 0 86 37 1188 779.6 0 1188 38 1011 779.6 0 1011 39 1863 779.6 0 1863 40 14 779.6 0 14 41 9 779.6 0 9 42 10 779.6 0 10 43 18 779.6 0 18 44 17 779.6 0 17 45 29 779.6 0 29 46 12 779.6 0 12 47 16 779.6 0 16 48 15 779.6 0 15 49 14 779.6 0 14 50 15 779.6 0 15 51 7 779.6 0 7 52 13 779.6 0 13 53 12 779.6 0 12 54 15 779.6 0 15 55 8 779.6 0 8 56 16 779.6 0 16 57 8 779.6 0 8 58 16 779.6 0 16 59 11 779.6 0 11 60 21 779.6 0 21 61 9 779.6 0 9 62 38 779.6 0 38 63 27 779.6 0 27 64 17 779.6 0 17 65 12 779.6 0 12 66 9 779.6 0 9 67 8 779.6 0 8 68 17 779.6 0 17 69 8 779.6 0 8 70 24 779.6 0 24 71 44 779.6 0 44 72 26 779.6 0 26 73 10 779.6 0 10 74 23 779.6 0 23 75 65 779.6 0 65 76 15 779.6 0 15 77 16 779.6 0 16 78 16 779.6 0 16 79 31 779.6 0 31 80 22 779.6 0 22 81 13 779.6 0 13 82 11 779.6 0 11 83 12 779.6 0 12 84 8 779.6 0 8 85 18 779.6 0 18 86 20 779.6 0 20 87 9 779.6 0 9 88 11 779.6 0 11 89 15 779.6 0 15 90 28 779.6 0 28 91 225 779.6 0 225 92 270 779.6 0 270 93 151 779.6 0 151 94 26 779.6 0 26 95 13 779.6 0 13 96 10 779.6 0 10 97 11 779.6 0 11 98 19 779.6 0 19 99 11 779.6 0 11 100 21 779.6 0 21 101 18 779.6 0 18 102 12 779.6 0 12 103 17 779.6 0 17 104 26 779.6 0 26 105 12 779.6 0 12 106 18 779.6 0 18 107 17 779.6 0 17 108 15 779.6 0 15 109 16 779.6 0 16 110 23 779.6 0 23 111 9 779.6 0 9 112 31 779.6 0 31 113 91 779.6 0 91 114 43 779.6 0 43 115 33 779.6 0 33 116 40 779.6 0 40 117 16 779.6 0 16 118 36 779.6 0 36 119 25 779.6 0 25 120 24 779.6 0 24 121 16 779.6 0 16 122 21 779.6 0 21 123 14 779.6 0 14 124 26 779.6 0 26 125 33 779.6 0 33 126 41 779.6 0 41 127 57 779.6 0 57 128 103 779.6 0 103 129 350 779.6 0 350 130 149 779.6 0 149 131 791 779.6 0 791 132 119 779.6 0 119 133 77 779.6 0 77 134 59 779.6 0 59 135 104 779.6 0 104 136 165 779.6 0 165 137 131 779.6 0 131 138 134 779.6 0 134 139 157 779.6 0 157 140 249 779.6 0 249 141 610 779.6 0 610 142 14187 779.6 0 14187 143 5519 779.6 0 5519 144 3251 779.6 0 3251 145 26 779.6 0 26 146 14 779.6 0 14 147 10 779.6 0 10 148 12 779.6 0 12 149 50 779.6 0 50 150 208 779.6 0 208 151 120 779.6 0 120 152 12 779.6 0 12 153 8 779.6 0 8 154 9 779.6 0 9 155 7 779.6 0 7 156 11 779.6 0 11 157 16 779.6 0 16 158 9 779.6 0 9 159 14 779.6 0 14 160 10 779.6 0 10 161 7 779.6 0 7 162 8 779.6 0 8 163 17 779.6 0 17 164 9 779.6 0 9 165 9 779.6 0 9 166 13 779.6 0 13 167 11 779.6 0 11 168 20 779.6 0 20 169 19 779.6 0 19 170 32 779.6 0 32 171 38 779.6 0 38 172 5 779.6 0 5 173 18 779.6 0 18 174 19 779.6 0 19 175 15 779.6 0 15 176 19 779.6 0 19 177 41 779.6 0 41 178 142 779.6 0 142 179 952 779.6 0 952 180 7 779.6 0 7 181 9 779.6 0 9 182 22 779.6 0 22 183 11 779.6 0 11 184 12 779.6 0 12 185 13 779.6 0 13 186 13 779.6 0 13 187 12 779.6 0 12 188 12 779.6 0 12 189 4 779.6 0 4 190 30 779.6 0 30 191 67 779.6 0 67 192 72 779.6 0 72 193 13 779.6 0 13 194 7 779.6 0 7 195 6 779.6 0 6 196 5 779.6 0 5 197 12 779.6 0 12 198 6 779.6 0 6 199 11 779.6 0 11 200 6 779.6 0 6 201 15 779.6 0 15 202 21 779.6 0 21 203 83 779.6 0 83 204 20 779.6 0 20 205 25 779.6 0 25 206 11 779.6 0 11 207 9 779.6 0 9 208 9 779.6 0 9 209 20 779.6 0 20 210 33 779.6 0 33 211 64 779.6 0 64 212 22 779.6 0 22 213 26 779.6 0 26 214 176 779.6 0 176 215 90 779.6 0 90 216 139 779.6 0 139 217 31 779.6 0 31 218 20 779.6 0 20 219 11 779.6 0 11 220 10 779.6 0 10 221 11 779.6 0 11 222 13 779.6 0 13 223 8 779.6 0 8 224 19 779.6 0 19 225 24 779.6 0 24 226 23 779.6 0 23 227 38 779.6 0 38 228 38 779.6 0 38 229 68 779.6 0 68 230 51 779.6 0 51 231 87 779.6 0 87 232 34 779.6 0 34 233 18 779.6 0 18 234 49 779.6 0 49 235 392 779.6 0 392 236 640 779.6 0 640 237 129 779.6 0 129 238 13 779.6 0 13 239 7 779.6 0 7 240 9 779.6 0 9 241 6 779.6 0 6 242 9 779.6 0 9 243 6 779.6 0 6 244 6 779.6 0 6 245 8 779.6 0 8 246 10 779.6 0 10 247 8 779.6 0 8 248 8 779.6 0 8 249 13 779.6 0 13 250 12 779.6 0 12 251 19 779.6 0 19 252 10 779.6 0 10 253 7 779.6 0 7 254 17 779.6 0 17 255 16 779.6 0 16 256 16 779.6 0 16 257 6 779.6 0 6 258 5 779.6 0 5 259 6 779.6 0 6 260 16 779.6 0 16 261 22 779.6 0 22 262 40 779.6 0 40 263 63 779.6 0 63 264 285 779.6 0 285 265 19 779.6 0 19 266 15 779.6 0 15 267 7 779.6 0 7 268 7 779.6 0 7 269 4 779.6 0 4 270 7 779.6 0 7 271 13 779.6 0 13 272 14 779.6 0 14 273 9 779.6 0 9 274 9 779.6 0 9 275 11 779.6 0 11 276 13 779.6 0 13 277 29 779.6 0 29 278 10 779.6 0 10 279 13 779.6 0 13 280 6 779.6 0 6 281 10 779.6 0 10 282 12 779.6 0 12 283 11 779.6 0 11 284 25 779.6 0 25 285 11 779.6 0 11 286 7 779.6 0 7 287 50 779.6 0 50 288 9 779.6 0 9 289 6 779.6 0 6 290 10 779.6 0 10 291 27 779.6 0 27 292 14 779.6 0 14 293 13 779.6 0 13 294 20 779.6 0 20 295 17 779.6 0 17 296 30 779.6 0 30 297 51 779.6 0 51 298 23 779.6 0 23 299 59 779.6 0 59 300 19 779.6 0 19 301 19 779.6 0 19 Finished in 2.13 s (42 us/read; 1.44 M reads/minute). === Summary === Total reads processed: 51,157 Reads with adapters: 7,117 (13.9%) Reads that were too short: 4 (0.0%) Reads written (passing filters): 7,113 (13.9%) Total basepairs processed: 10,790,465 bp Total written (filtered): 1,965,127 bp (18.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 7117 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 5 0.0 3 0 0 4 1 16 25 0.0 3 0 23 2 17 3809 0.0 3 3698 108 2 1 18 3140 0.0 3 3084 47 8 1 19 27 0.0 3 2 9 13 3 20 88 0.0 3 83 1 2 2 21 9 0.0 3 4 0 0 5 22 1 0.0 3 0 0 0 1 28 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 76 1 0.0 3 1 128 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 231 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.29 s (41 us/read; 1.47 M reads/minute). === Summary === Total reads processed: 7,113 Reads with adapters: 270 (3.8%) Reads written (passing filters): 7,113 (100.0%) Total basepairs processed: 1,965,127 bp Total written (filtered): 1,964,314 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 270 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 6.7% C: 81.5% G: 10.4% T: 1.5% none/other: 0.0% WARNING: The adapter is preceded by "C" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "C" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 268 111.1 0 268 4 1 27.8 0 1 5 1 6.9 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.