This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CATTAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 104.83 s (33 us/read; 1.82 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 184,209 (5.8%) Reads that were too short: 29,165 (0.9%) Reads written (passing filters): 155,044 (4.9%) Total basepairs processed: 958,672,960 bp Total written (filtered): 26,104,005 bp (2.7%) === Adapter 1 === Sequence: CATTAG; Type: regular 5'; Length: 6; Trimmed: 184209 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 15 777.6 0 15 7 135 777.6 0 135 8 83 777.6 0 83 9 31 777.6 0 31 10 12 777.6 0 12 11 11 777.6 0 11 12 13 777.6 0 13 13 11 777.6 0 11 14 11 777.6 0 11 15 13 777.6 0 13 16 16 777.6 0 16 17 15 777.6 0 15 18 12 777.6 0 12 19 17 777.6 0 17 20 15 777.6 0 15 21 23 777.6 0 23 22 11 777.6 0 11 23 16 777.6 0 16 24 16 777.6 0 16 25 19 777.6 0 19 26 19 777.6 0 19 27 236 777.6 0 236 28 318 777.6 0 318 29 28 777.6 0 28 30 13 777.6 0 13 31 15 777.6 0 15 32 26 777.6 0 26 33 19 777.6 0 19 34 368 777.6 0 368 35 61 777.6 0 61 36 17 777.6 0 17 37 26 777.6 0 26 38 17 777.6 0 17 39 17 777.6 0 17 40 15 777.6 0 15 41 27 777.6 0 27 42 24 777.6 0 24 43 20 777.6 0 20 44 314 777.6 0 314 45 67 777.6 0 67 46 17 777.6 0 17 47 11 777.6 0 11 48 10 777.6 0 10 49 10 777.6 0 10 50 22 777.6 0 22 51 16 777.6 0 16 52 22 777.6 0 22 53 25 777.6 0 25 54 22 777.6 0 22 55 32 777.6 0 32 56 22 777.6 0 22 57 34 777.6 0 34 58 507 777.6 0 507 59 47 777.6 0 47 60 42 777.6 0 42 61 56 777.6 0 56 62 33 777.6 0 33 63 26 777.6 0 26 64 20 777.6 0 20 65 30 777.6 0 30 66 23 777.6 0 23 67 43 777.6 0 43 68 78 777.6 0 78 69 116 777.6 0 116 70 240 777.6 0 240 71 500 777.6 0 500 72 9665 777.6 0 9665 73 2976 777.6 0 2976 74 3614 777.6 0 3614 75 76 777.6 0 76 76 189 777.6 0 189 77 185 777.6 0 185 78 33 777.6 0 33 79 58 777.6 0 58 80 68 777.6 0 68 81 74 777.6 0 74 82 124 777.6 0 124 83 82 777.6 0 82 84 94 777.6 0 94 85 82 777.6 0 82 86 103 777.6 0 103 87 50 777.6 0 50 88 117 777.6 0 117 89 843 777.6 0 843 90 828 777.6 0 828 91 112 777.6 0 112 92 806 777.6 0 806 93 68 777.6 0 68 94 86 777.6 0 86 95 119 777.6 0 119 96 105 777.6 0 105 97 203 777.6 0 203 98 23 777.6 0 23 99 36 777.6 0 36 100 101 777.6 0 101 101 55 777.6 0 55 102 21 777.6 0 21 103 40 777.6 0 40 104 28 777.6 0 28 105 11 777.6 0 11 106 48 777.6 0 48 107 34 777.6 0 34 108 39 777.6 0 39 109 158 777.6 0 158 110 171 777.6 0 171 111 61 777.6 0 61 112 148 777.6 0 148 113 281 777.6 0 281 114 271 777.6 0 271 115 86 777.6 0 86 116 77 777.6 0 77 117 98 777.6 0 98 118 94 777.6 0 94 119 208 777.6 0 208 120 64 777.6 0 64 121 109 777.6 0 109 122 95 777.6 0 95 123 132 777.6 0 132 124 122 777.6 0 122 125 122 777.6 0 122 126 166 777.6 0 166 127 275 777.6 0 275 128 469 777.6 0 469 129 730 777.6 0 730 130 1846 777.6 0 1846 131 388 777.6 0 388 132 485 777.6 0 485 133 1186 777.6 0 1186 134 797 777.6 0 797 135 587 777.6 0 587 136 1131 777.6 0 1131 137 766 777.6 0 766 138 1650 777.6 0 1650 139 577 777.6 0 577 140 1077 777.6 0 1077 141 1483 777.6 0 1483 142 1466 777.6 0 1466 143 19006 777.6 0 19006 144 12633 777.6 0 12633 145 71412 777.6 0 71412 146 422 777.6 0 422 147 108 777.6 0 108 148 130 777.6 0 130 149 117 777.6 0 117 150 172 777.6 0 172 151 73 777.6 0 73 152 55 777.6 0 55 153 74 777.6 0 74 154 75 777.6 0 75 155 167 777.6 0 167 156 387 777.6 0 387 157 1717 777.6 0 1717 158 201 777.6 0 201 159 86 777.6 0 86 160 79 777.6 0 79 161 88 777.6 0 88 162 155 777.6 0 155 163 680 777.6 0 680 164 269 777.6 0 269 165 1013 777.6 0 1013 166 114 777.6 0 114 167 108 777.6 0 108 168 113 777.6 0 113 169 80 777.6 0 80 170 70 777.6 0 70 171 39 777.6 0 39 172 48 777.6 0 48 173 67 777.6 0 67 174 91 777.6 0 91 175 105 777.6 0 105 176 91 777.6 0 91 177 74 777.6 0 74 178 38 777.6 0 38 179 57 777.6 0 57 180 76 777.6 0 76 181 80 777.6 0 80 182 104 777.6 0 104 183 107 777.6 0 107 184 107 777.6 0 107 185 75 777.6 0 75 186 86 777.6 0 86 187 92 777.6 0 92 188 88 777.6 0 88 189 67 777.6 0 67 190 89 777.6 0 89 191 106 777.6 0 106 192 117 777.6 0 117 193 86 777.6 0 86 194 175 777.6 0 175 195 150 777.6 0 150 196 89 777.6 0 89 197 135 777.6 0 135 198 100 777.6 0 100 199 58 777.6 0 58 200 67 777.6 0 67 201 58 777.6 0 58 202 185 777.6 0 185 203 333 777.6 0 333 204 201 777.6 0 201 205 90 777.6 0 90 206 108 777.6 0 108 207 94 777.6 0 94 208 141 777.6 0 141 209 271 777.6 0 271 210 199 777.6 0 199 211 171 777.6 0 171 212 244 777.6 0 244 213 202 777.6 0 202 214 282 777.6 0 282 215 310 777.6 0 310 216 612 777.6 0 612 217 276 777.6 0 276 218 122 777.6 0 122 219 92 777.6 0 92 220 78 777.6 0 78 221 91 777.6 0 91 222 133 777.6 0 133 223 259 777.6 0 259 224 69 777.6 0 69 225 91 777.6 0 91 226 245 777.6 0 245 227 120 777.6 0 120 228 152 777.6 0 152 229 218 777.6 0 218 230 268 777.6 0 268 231 703 777.6 0 703 232 144 777.6 0 144 233 119 777.6 0 119 234 167 777.6 0 167 235 191 777.6 0 191 236 180 777.6 0 180 237 209 777.6 0 209 238 129 777.6 0 129 239 140 777.6 0 140 240 148 777.6 0 148 241 136 777.6 0 136 242 106 777.6 0 106 243 246 777.6 0 246 244 175 777.6 0 175 245 146 777.6 0 146 246 155 777.6 0 155 247 126 777.6 0 126 248 139 777.6 0 139 249 148 777.6 0 148 250 167 777.6 0 167 251 128 777.6 0 128 252 104 777.6 0 104 253 142 777.6 0 142 254 161 777.6 0 161 255 173 777.6 0 173 256 4033 777.6 0 4033 257 1916 777.6 0 1916 258 2294 777.6 0 2294 259 131 777.6 0 131 260 108 777.6 0 108 261 110 777.6 0 110 262 75 777.6 0 75 263 59 777.6 0 59 264 68 777.6 0 68 265 138 777.6 0 138 266 150 777.6 0 150 267 339 777.6 0 339 268 178 777.6 0 178 269 107 777.6 0 107 270 158 777.6 0 158 271 138 777.6 0 138 272 77 777.6 0 77 273 105 777.6 0 105 274 129 777.6 0 129 275 188 777.6 0 188 276 130 777.6 0 130 277 80 777.6 0 80 278 121 777.6 0 121 279 147 777.6 0 147 280 889 777.6 0 889 281 488 777.6 0 488 282 625 777.6 0 625 283 1271 777.6 0 1271 284 121 777.6 0 121 285 140 777.6 0 140 286 154 777.6 0 154 287 274 777.6 0 274 288 430 777.6 0 430 289 203 777.6 0 203 290 139 777.6 0 139 291 120 777.6 0 120 292 130 777.6 0 130 293 361 777.6 0 361 294 375 777.6 0 375 295 281 777.6 0 281 296 588 777.6 0 588 297 273 777.6 0 273 298 205 777.6 0 205 299 406 777.6 0 406 300 513 777.6 0 513 301 161 777.6 0 161 Finished in 7.04 s (45 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 155,044 Reads with adapters: 93 (0.1%) Reads that were too short: 86 (0.1%) Reads written (passing filters): 7 (0.0%) Total basepairs processed: 26,104,005 bp Total written (filtered): 1,187 bp (0.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 93 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 22 1 0.0 4 0 1 56 1 0.0 4 0 0 0 0 1 57 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 0 1 84 1 0.0 4 0 0 0 0 1 85 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 0 0 0 1 90 2 0.0 4 0 0 0 0 2 97 3 0.0 4 0 0 0 0 3 98 1 0.0 4 0 0 0 0 1 99 2 0.0 4 0 0 0 1 1 109 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 0 1 122 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 1 0.0 4 0 0 0 0 1 129 2 0.0 4 0 0 0 0 2 136 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 141 2 0.0 4 0 0 0 0 2 146 45 0.0 4 0 0 0 0 45 147 11 0.0 4 0 0 0 3 8 158 1 0.0 4 0 0 0 0 1 159 2 0.0 4 0 0 0 0 2 160 1 0.0 4 0 0 0 0 1 162 1 0.0 4 0 0 0 0 1 171 1 0.0 4 0 0 0 0 1 180 1 0.0 4 0 0 0 0 1 192 1 0.0 4 0 0 0 0 1 195 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (1429 us/read; 0.04 M reads/minute). === Summary === Total reads processed: 7 Reads with adapters: 0 (0.0%) Reads written (passing filters): 7 (100.0%) Total basepairs processed: 1,187 bp Total written (filtered): 1,187 bp (100.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 0 times.