This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGGATG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 103.63 s (33 us/read; 1.84 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 132,051 (4.1%) Reads that were too short: 13,929 (0.4%) Reads written (passing filters): 118,122 (3.7%) Total basepairs processed: 958,672,960 bp Total written (filtered): 21,771,349 bp (2.3%) === Adapter 1 === Sequence: AGGATG; Type: regular 5'; Length: 6; Trimmed: 132051 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 88 777.6 0 88 7 468 777.6 0 468 8 13833 777.6 0 13833 9 31 777.6 0 31 10 10 777.6 0 10 11 8 777.6 0 8 12 10 777.6 0 10 13 13 777.6 0 13 14 11 777.6 0 11 15 13 777.6 0 13 16 7 777.6 0 7 17 8 777.6 0 8 18 27 777.6 0 27 19 13 777.6 0 13 20 22 777.6 0 22 21 16 777.6 0 16 22 11 777.6 0 11 23 11 777.6 0 11 24 12 777.6 0 12 25 12 777.6 0 12 26 7 777.6 0 7 27 13 777.6 0 13 28 13 777.6 0 13 29 9 777.6 0 9 30 14 777.6 0 14 31 12 777.6 0 12 32 11 777.6 0 11 33 12 777.6 0 12 34 8 777.6 0 8 35 11 777.6 0 11 36 11 777.6 0 11 37 18 777.6 0 18 38 15 777.6 0 15 39 11 777.6 0 11 40 13 777.6 0 13 41 15 777.6 0 15 42 10 777.6 0 10 43 10 777.6 0 10 44 13 777.6 0 13 45 11 777.6 0 11 46 18 777.6 0 18 47 10 777.6 0 10 48 25 777.6 0 25 49 13 777.6 0 13 50 71 777.6 0 71 51 48 777.6 0 48 52 11 777.6 0 11 53 26 777.6 0 26 54 41 777.6 0 41 55 25 777.6 0 25 56 73 777.6 0 73 57 16 777.6 0 16 58 35 777.6 0 35 59 19 777.6 0 19 60 61 777.6 0 61 61 27 777.6 0 27 62 54 777.6 0 54 63 56 777.6 0 56 64 124 777.6 0 124 65 168 777.6 0 168 66 293 777.6 0 293 67 628 777.6 0 628 68 124 777.6 0 124 69 185 777.6 0 185 70 443 777.6 0 443 71 215 777.6 0 215 72 155 777.6 0 155 73 315 777.6 0 315 74 243 777.6 0 243 75 420 777.6 0 420 76 209 777.6 0 209 77 323 777.6 0 323 78 350 777.6 0 350 79 896 777.6 0 896 80 14524 777.6 0 14524 81 10127 777.6 0 10127 82 20528 777.6 0 20528 83 141 777.6 0 141 84 23 777.6 0 23 85 13 777.6 0 13 86 12 777.6 0 12 87 16 777.6 0 16 88 14 777.6 0 14 89 7 777.6 0 7 90 32 777.6 0 32 91 29 777.6 0 29 92 93 777.6 0 93 93 78 777.6 0 78 94 31 777.6 0 31 95 60 777.6 0 60 96 25 777.6 0 25 97 154 777.6 0 154 98 20 777.6 0 20 99 23 777.6 0 23 100 32 777.6 0 32 101 34 777.6 0 34 102 7 777.6 0 7 103 14 777.6 0 14 104 24 777.6 0 24 105 24 777.6 0 24 106 17 777.6 0 17 107 21 777.6 0 21 108 22 777.6 0 22 109 9 777.6 0 9 110 19 777.6 0 19 111 24 777.6 0 24 112 11 777.6 0 11 113 29 777.6 0 29 114 12 777.6 0 12 115 10 777.6 0 10 116 45 777.6 0 45 117 68 777.6 0 68 118 55 777.6 0 55 119 161 777.6 0 161 120 93 777.6 0 93 121 127 777.6 0 127 122 41 777.6 0 41 123 41 777.6 0 41 124 39 777.6 0 39 125 270 777.6 0 270 126 30 777.6 0 30 127 170 777.6 0 170 128 68 777.6 0 68 129 374 777.6 0 374 130 28 777.6 0 28 131 55 777.6 0 55 132 25 777.6 0 25 133 23 777.6 0 23 134 29 777.6 0 29 135 17 777.6 0 17 136 21 777.6 0 21 137 35 777.6 0 35 138 33 777.6 0 33 139 38 777.6 0 38 140 21 777.6 0 21 141 29 777.6 0 29 142 36 777.6 0 36 143 32 777.6 0 32 144 23 777.6 0 23 145 82 777.6 0 82 146 83 777.6 0 83 147 35 777.6 0 35 148 257 777.6 0 257 149 71 777.6 0 71 150 240 777.6 0 240 151 14 777.6 0 14 152 21 777.6 0 21 153 37 777.6 0 37 154 21 777.6 0 21 155 26 777.6 0 26 156 19 777.6 0 19 157 27 777.6 0 27 158 17 777.6 0 17 159 34 777.6 0 34 160 25 777.6 0 25 161 29 777.6 0 29 162 36 777.6 0 36 163 42 777.6 0 42 164 66 777.6 0 66 165 37 777.6 0 37 166 40 777.6 0 40 167 75 777.6 0 75 168 70 777.6 0 70 169 50 777.6 0 50 170 76 777.6 0 76 171 138 777.6 0 138 172 85 777.6 0 85 173 105 777.6 0 105 174 221 777.6 0 221 175 372 777.6 0 372 176 117 777.6 0 117 177 173 777.6 0 173 178 225 777.6 0 225 179 168 777.6 0 168 180 181 777.6 0 181 181 267 777.6 0 267 182 221 777.6 0 221 183 302 777.6 0 302 184 201 777.6 0 201 185 288 777.6 0 288 186 600 777.6 0 600 187 6051 777.6 0 6051 188 20343 777.6 0 20343 189 4814 777.6 0 4814 190 8472 777.6 0 8472 191 582 777.6 0 582 192 306 777.6 0 306 193 620 777.6 0 620 194 426 777.6 0 426 195 1494 777.6 0 1494 196 517 777.6 0 517 197 25 777.6 0 25 198 35 777.6 0 35 199 38 777.6 0 38 200 147 777.6 0 147 201 191 777.6 0 191 202 22 777.6 0 22 203 33 777.6 0 33 204 87 777.6 0 87 205 88 777.6 0 88 206 813 777.6 0 813 207 440 777.6 0 440 208 974 777.6 0 974 209 36 777.6 0 36 210 47 777.6 0 47 211 138 777.6 0 138 212 231 777.6 0 231 213 420 777.6 0 420 214 69 777.6 0 69 215 31 777.6 0 31 216 28 777.6 0 28 217 26 777.6 0 26 218 29 777.6 0 29 219 25 777.6 0 25 220 23 777.6 0 23 221 148 777.6 0 148 222 22 777.6 0 22 223 93 777.6 0 93 224 51 777.6 0 51 225 43 777.6 0 43 226 39 777.6 0 39 227 33 777.6 0 33 228 47 777.6 0 47 229 57 777.6 0 57 230 37 777.6 0 37 231 20 777.6 0 20 232 63 777.6 0 63 233 35 777.6 0 35 234 24 777.6 0 24 235 40 777.6 0 40 236 35 777.6 0 35 237 42 777.6 0 42 238 26 777.6 0 26 239 35 777.6 0 35 240 52 777.6 0 52 241 143 777.6 0 143 242 60 777.6 0 60 243 18 777.6 0 18 244 28 777.6 0 28 245 36 777.6 0 36 246 17 777.6 0 17 247 19 777.6 0 19 248 82 777.6 0 82 249 27 777.6 0 27 250 36 777.6 0 36 251 60 777.6 0 60 252 60 777.6 0 60 253 52 777.6 0 52 254 24 777.6 0 24 255 50 777.6 0 50 256 44 777.6 0 44 257 66 777.6 0 66 258 48 777.6 0 48 259 28 777.6 0 28 260 27 777.6 0 27 261 46 777.6 0 46 262 46 777.6 0 46 263 52 777.6 0 52 264 51 777.6 0 51 265 46 777.6 0 46 266 39 777.6 0 39 267 33 777.6 0 33 268 114 777.6 0 114 269 111 777.6 0 111 270 63 777.6 0 63 271 608 777.6 0 608 272 1713 777.6 0 1713 273 1152 777.6 0 1152 274 275 777.6 0 275 275 66 777.6 0 66 276 45 777.6 0 45 277 43 777.6 0 43 278 21 777.6 0 21 279 17 777.6 0 17 280 30 777.6 0 30 281 94 777.6 0 94 282 274 777.6 0 274 283 132 777.6 0 132 284 266 777.6 0 266 285 22 777.6 0 22 286 21 777.6 0 21 287 26 777.6 0 26 288 48 777.6 0 48 289 55 777.6 0 55 290 70 777.6 0 70 291 255 777.6 0 255 292 638 777.6 0 638 293 565 777.6 0 565 294 786 777.6 0 786 295 124 777.6 0 124 296 52 777.6 0 52 297 73 777.6 0 73 298 178 777.6 0 178 299 115 777.6 0 115 300 99 777.6 0 99 301 38 777.6 0 38 Finished in 4.87 s (41 us/read; 1.46 M reads/minute). === Summary === Total reads processed: 118,122 Reads with adapters: 92 (0.1%) Reads that were too short: 22 (0.0%) Reads written (passing filters): 70 (0.1%) Total basepairs processed: 21,771,349 bp Total written (filtered): 9,078 bp (0.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 92 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 2 18 1 0.0 3 0 0 0 1 20 2 0.0 3 1 0 0 1 32 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 1 52 10 0.0 3 8 2 63 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 67 2 0.0 3 0 0 0 2 73 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 1 86 5 0.0 3 0 0 0 5 87 3 0.0 3 0 0 0 3 89 1 0.0 3 0 0 0 1 91 3 0.0 3 0 0 0 3 92 4 0.0 3 0 0 0 4 95 4 0.0 3 0 0 0 4 96 1 0.0 3 0 0 0 1 101 2 0.0 3 0 0 0 2 104 2 0.0 3 0 0 0 2 109 1 0.0 3 0 0 0 1 111 9 0.0 3 0 0 0 9 112 1 0.0 3 0 0 0 1 114 16 0.0 3 0 0 0 16 122 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 134 2 0.0 3 0 0 0 2 137 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 197 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 0 1 213 3 0.0 3 0 0 0 3 216 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (143 us/read; 0.42 M reads/minute). === Summary === Total reads processed: 70 Reads with adapters: 2 (2.9%) Reads written (passing filters): 70 (100.0%) Total basepairs processed: 9,078 bp Total written (filtered): 9,072 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 2 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 0.0% C: 50.0% G: 50.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2 1.1 0 2