This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATGGAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 174.53 s (33 us/read; 1.83 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 96,539 (1.8%) Reads that were too short: 14,904 (0.3%) Reads written (passing filters): 81,635 (1.5%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 20,297,739 bp (1.3%) === Adapter 1 === Sequence: ATGGAT; Type: regular 5'; Length: 6; Trimmed: 96539 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1360 1302.2 0 1360 7 41958 1302.2 0 41958 8 126 1302.2 0 126 9 3979 1302.2 0 3979 10 12 1302.2 0 12 11 12 1302.2 0 12 12 14 1302.2 0 14 13 11 1302.2 0 11 14 7 1302.2 0 7 15 8 1302.2 0 8 16 26 1302.2 0 26 17 59 1302.2 0 59 18 54 1302.2 0 54 19 14 1302.2 0 14 20 21 1302.2 0 21 21 13 1302.2 0 13 22 11 1302.2 0 11 23 11 1302.2 0 11 24 13 1302.2 0 13 25 11 1302.2 0 11 26 13 1302.2 0 13 27 19 1302.2 0 19 28 13 1302.2 0 13 29 22 1302.2 0 22 30 15 1302.2 0 15 31 6 1302.2 0 6 32 13 1302.2 0 13 33 13 1302.2 0 13 34 10 1302.2 0 10 35 8 1302.2 0 8 36 9 1302.2 0 9 37 17 1302.2 0 17 38 15 1302.2 0 15 39 11 1302.2 0 11 40 83 1302.2 0 83 41 19 1302.2 0 19 42 19 1302.2 0 19 43 16 1302.2 0 16 44 14 1302.2 0 14 45 14 1302.2 0 14 46 23 1302.2 0 23 47 101 1302.2 0 101 48 437 1302.2 0 437 49 15 1302.2 0 15 50 15 1302.2 0 15 51 12 1302.2 0 12 52 14 1302.2 0 14 53 16 1302.2 0 16 54 14 1302.2 0 14 55 25 1302.2 0 25 56 281 1302.2 0 281 57 226 1302.2 0 226 58 314 1302.2 0 314 59 30 1302.2 0 30 60 11 1302.2 0 11 61 13 1302.2 0 13 62 9 1302.2 0 9 63 25 1302.2 0 25 64 12 1302.2 0 12 65 12 1302.2 0 12 66 18 1302.2 0 18 67 22 1302.2 0 22 68 20 1302.2 0 20 69 9 1302.2 0 9 70 18 1302.2 0 18 71 15 1302.2 0 15 72 12 1302.2 0 12 73 11 1302.2 0 11 74 21 1302.2 0 21 75 18 1302.2 0 18 76 21 1302.2 0 21 77 27 1302.2 0 27 78 79 1302.2 0 79 79 88 1302.2 0 88 80 237 1302.2 0 237 81 570 1302.2 0 570 82 375 1302.2 0 375 83 156 1302.2 0 156 84 216 1302.2 0 216 85 72 1302.2 0 72 86 61 1302.2 0 61 87 88 1302.2 0 88 88 53 1302.2 0 53 89 107 1302.2 0 107 90 99 1302.2 0 99 91 99 1302.2 0 99 92 112 1302.2 0 112 93 215 1302.2 0 215 94 3474 1302.2 0 3474 95 4500 1302.2 0 4500 96 6365 1302.2 0 6365 97 289 1302.2 0 289 98 178 1302.2 0 178 99 50 1302.2 0 50 100 148 1302.2 0 148 101 295 1302.2 0 295 102 58 1302.2 0 58 103 67 1302.2 0 67 104 20 1302.2 0 20 105 20 1302.2 0 20 106 32 1302.2 0 32 107 49 1302.2 0 49 108 48 1302.2 0 48 109 50 1302.2 0 50 110 367 1302.2 0 367 111 101 1302.2 0 101 112 247 1302.2 0 247 113 45 1302.2 0 45 114 35 1302.2 0 35 115 182 1302.2 0 182 116 78 1302.2 0 78 117 86 1302.2 0 86 118 148 1302.2 0 148 119 168 1302.2 0 168 120 124 1302.2 0 124 121 74 1302.2 0 74 122 73 1302.2 0 73 123 107 1302.2 0 107 124 98 1302.2 0 98 125 81 1302.2 0 81 126 98 1302.2 0 98 127 81 1302.2 0 81 128 77 1302.2 0 77 129 51 1302.2 0 51 130 90 1302.2 0 90 131 267 1302.2 0 267 132 364 1302.2 0 364 133 383 1302.2 0 383 134 128 1302.2 0 128 135 97 1302.2 0 97 136 185 1302.2 0 185 137 347 1302.2 0 347 138 356 1302.2 0 356 139 486 1302.2 0 486 140 302 1302.2 0 302 141 140 1302.2 0 140 142 76 1302.2 0 76 143 73 1302.2 0 73 144 97 1302.2 0 97 145 413 1302.2 0 413 146 340 1302.2 0 340 147 87 1302.2 0 87 148 56 1302.2 0 56 149 43 1302.2 0 43 150 55 1302.2 0 55 151 43 1302.2 0 43 152 36 1302.2 0 36 153 30 1302.2 0 30 154 66 1302.2 0 66 155 62 1302.2 0 62 156 99 1302.2 0 99 157 166 1302.2 0 166 158 174 1302.2 0 174 159 153 1302.2 0 153 160 159 1302.2 0 159 161 91 1302.2 0 91 162 63 1302.2 0 63 163 75 1302.2 0 75 164 75 1302.2 0 75 165 129 1302.2 0 129 166 157 1302.2 0 157 167 135 1302.2 0 135 168 112 1302.2 0 112 169 89 1302.2 0 89 170 154 1302.2 0 154 171 90 1302.2 0 90 172 105 1302.2 0 105 173 61 1302.2 0 61 174 135 1302.2 0 135 175 98 1302.2 0 98 176 88 1302.2 0 88 177 57 1302.2 0 57 178 35 1302.2 0 35 179 90 1302.2 0 90 180 173 1302.2 0 173 181 236 1302.2 0 236 182 154 1302.2 0 154 183 140 1302.2 0 140 184 126 1302.2 0 126 185 141 1302.2 0 141 186 168 1302.2 0 168 187 224 1302.2 0 224 188 110 1302.2 0 110 189 32 1302.2 0 32 190 55 1302.2 0 55 191 150 1302.2 0 150 192 499 1302.2 0 499 193 284 1302.2 0 284 194 871 1302.2 0 871 195 141 1302.2 0 141 196 58 1302.2 0 58 197 51 1302.2 0 51 198 118 1302.2 0 118 199 104 1302.2 0 104 200 80 1302.2 0 80 201 31 1302.2 0 31 202 32 1302.2 0 32 203 106 1302.2 0 106 204 268 1302.2 0 268 205 600 1302.2 0 600 206 485 1302.2 0 485 207 315 1302.2 0 315 208 87 1302.2 0 87 209 68 1302.2 0 68 210 89 1302.2 0 89 211 162 1302.2 0 162 212 225 1302.2 0 225 213 260 1302.2 0 260 214 190 1302.2 0 190 215 153 1302.2 0 153 216 117 1302.2 0 117 217 183 1302.2 0 183 218 219 1302.2 0 219 219 168 1302.2 0 168 220 175 1302.2 0 175 221 71 1302.2 0 71 222 44 1302.2 0 44 223 104 1302.2 0 104 224 191 1302.2 0 191 225 154 1302.2 0 154 226 179 1302.2 0 179 227 225 1302.2 0 225 228 263 1302.2 0 263 229 231 1302.2 0 231 230 115 1302.2 0 115 231 99 1302.2 0 99 232 86 1302.2 0 86 233 63 1302.2 0 63 234 122 1302.2 0 122 235 183 1302.2 0 183 236 222 1302.2 0 222 237 147 1302.2 0 147 238 195 1302.2 0 195 239 186 1302.2 0 186 240 124 1302.2 0 124 241 44 1302.2 0 44 242 37 1302.2 0 37 243 70 1302.2 0 70 244 134 1302.2 0 134 245 124 1302.2 0 124 246 78 1302.2 0 78 247 133 1302.2 0 133 248 56 1302.2 0 56 249 55 1302.2 0 55 250 20 1302.2 0 20 251 36 1302.2 0 36 252 98 1302.2 0 98 253 170 1302.2 0 170 254 190 1302.2 0 190 255 106 1302.2 0 106 256 148 1302.2 0 148 257 185 1302.2 0 185 258 113 1302.2 0 113 259 108 1302.2 0 108 260 39 1302.2 0 39 261 153 1302.2 0 153 262 203 1302.2 0 203 263 254 1302.2 0 254 264 149 1302.2 0 149 265 200 1302.2 0 200 266 185 1302.2 0 185 267 236 1302.2 0 236 268 171 1302.2 0 171 269 64 1302.2 0 64 270 24 1302.2 0 24 271 100 1302.2 0 100 272 400 1302.2 0 400 273 210 1302.2 0 210 274 157 1302.2 0 157 275 127 1302.2 0 127 276 89 1302.2 0 89 277 55 1302.2 0 55 278 83 1302.2 0 83 279 155 1302.2 0 155 280 189 1302.2 0 189 281 93 1302.2 0 93 282 70 1302.2 0 70 283 83 1302.2 0 83 284 140 1302.2 0 140 285 148 1302.2 0 148 286 177 1302.2 0 177 287 184 1302.2 0 184 288 289 1302.2 0 289 289 83 1302.2 0 83 290 90 1302.2 0 90 291 123 1302.2 0 123 292 171 1302.2 0 171 293 223 1302.2 0 223 294 128 1302.2 0 128 295 66 1302.2 0 66 296 51 1302.2 0 51 297 85 1302.2 0 85 298 160 1302.2 0 160 299 181 1302.2 0 181 300 153 1302.2 0 153 301 152 1302.2 0 152 Finished in 4.13 s (51 us/read; 1.19 M reads/minute). === Summary === Total reads processed: 81,635 Reads with adapters: 4,416 (5.4%) Reads that were too short: 10 (0.0%) Reads written (passing filters): 4,406 (5.4%) Total basepairs processed: 20,297,739 bp Total written (filtered): 1,210,158 bp (6.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 4416 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 4 0.0 3 0 0 4 16 27 0.0 3 0 25 1 1 17 3860 0.0 3 3806 53 1 18 39 0.0 3 0 5 34 19 44 0.0 3 1 4 13 26 20 419 0.0 3 410 8 0 1 22 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 56 1 0.0 3 1 62 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 92 3 0.0 3 0 0 0 3 100 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 282 3 0.0 3 0 0 0 3 283 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.21 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 4,406 Reads with adapters: 297 (6.7%) Reads written (passing filters): 4,406 (100.0%) Total basepairs processed: 1,210,158 bp Total written (filtered): 1,209,249 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 297 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 50.5% C: 24.2% G: 16.5% T: 8.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 282 68.8 0 282 4 14 17.2 0 14 7 1 0.3 0 1