This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TAATGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 176.51 s (33 us/read; 1.81 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 497,859 (9.3%) Reads that were too short: 234,542 (4.4%) Reads written (passing filters): 263,317 (4.9%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 44,778,063 bp (2.8%) === Adapter 1 === Sequence: TAATGA; Type: regular 5'; Length: 6; Trimmed: 497859 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 153 1302.2 0 153 7 1322 1302.2 0 1322 8 7777 1302.2 0 7777 9 31 1302.2 0 31 10 23 1302.2 0 23 11 16 1302.2 0 16 12 14 1302.2 0 14 13 27 1302.2 0 27 14 17 1302.2 0 17 15 17 1302.2 0 17 16 2 1302.2 0 2 17 21 1302.2 0 21 18 21 1302.2 0 21 19 13 1302.2 0 13 20 22 1302.2 0 22 21 12 1302.2 0 12 22 21 1302.2 0 21 23 64 1302.2 0 64 24 23 1302.2 0 23 25 17 1302.2 0 17 26 17 1302.2 0 17 27 64 1302.2 0 64 28 22 1302.2 0 22 29 200 1302.2 0 200 30 199 1302.2 0 199 31 26 1302.2 0 26 32 10 1302.2 0 10 33 19 1302.2 0 19 34 24 1302.2 0 24 35 19 1302.2 0 19 36 109 1302.2 0 109 37 31 1302.2 0 31 38 30 1302.2 0 30 39 21 1302.2 0 21 40 21 1302.2 0 21 41 16 1302.2 0 16 42 21 1302.2 0 21 43 69 1302.2 0 69 44 42 1302.2 0 42 45 53 1302.2 0 53 46 33 1302.2 0 33 47 21 1302.2 0 21 48 12 1302.2 0 12 49 23 1302.2 0 23 50 24 1302.2 0 24 51 10 1302.2 0 10 52 18 1302.2 0 18 53 18 1302.2 0 18 54 12 1302.2 0 12 55 17 1302.2 0 17 56 20 1302.2 0 20 57 100 1302.2 0 100 58 33 1302.2 0 33 59 19 1302.2 0 19 60 22 1302.2 0 22 61 53 1302.2 0 53 62 24 1302.2 0 24 63 33 1302.2 0 33 64 19 1302.2 0 19 65 25 1302.2 0 25 66 30 1302.2 0 30 67 30 1302.2 0 30 68 23 1302.2 0 23 69 35 1302.2 0 35 70 427 1302.2 0 427 71 149 1302.2 0 149 72 362 1302.2 0 362 73 108 1302.2 0 108 74 165 1302.2 0 165 75 299 1302.2 0 299 76 2278 1302.2 0 2278 77 322 1302.2 0 322 78 835 1302.2 0 835 79 2624 1302.2 0 2624 80 3164 1302.2 0 3164 81 2444 1302.2 0 2444 82 4827 1302.2 0 4827 83 7262 1302.2 0 7262 84 1900 1302.2 0 1900 85 2195 1302.2 0 2195 86 1251 1302.2 0 1251 87 442 1302.2 0 442 88 692 1302.2 0 692 89 1418 1302.2 0 1418 90 202 1302.2 0 202 91 460 1302.2 0 460 92 2923 1302.2 0 2923 93 1410 1302.2 0 1410 94 2548 1302.2 0 2548 95 1639 1302.2 0 1639 96 404 1302.2 0 404 97 611 1302.2 0 611 98 2536 1302.2 0 2536 99 2397 1302.2 0 2397 100 1536 1302.2 0 1536 101 481 1302.2 0 481 102 774 1302.2 0 774 103 343 1302.2 0 343 104 476 1302.2 0 476 105 1252 1302.2 0 1252 106 338 1302.2 0 338 107 252 1302.2 0 252 108 825 1302.2 0 825 109 2896 1302.2 0 2896 110 584 1302.2 0 584 111 968 1302.2 0 968 112 1312 1302.2 0 1312 113 1233 1302.2 0 1233 114 2392 1302.2 0 2392 115 2086 1302.2 0 2086 116 2139 1302.2 0 2139 117 4351 1302.2 0 4351 118 2282 1302.2 0 2282 119 1094 1302.2 0 1094 120 1271 1302.2 0 1271 121 1134 1302.2 0 1134 122 1224 1302.2 0 1224 123 1291 1302.2 0 1291 124 7423 1302.2 0 7423 125 12152 1302.2 0 12152 126 19927 1302.2 0 19927 127 1493 1302.2 0 1493 128 930 1302.2 0 930 129 1999 1302.2 0 1999 130 2670 1302.2 0 2670 131 2256 1302.2 0 2256 132 1170 1302.2 0 1170 133 1446 1302.2 0 1446 134 1623 1302.2 0 1623 135 1991 1302.2 0 1991 136 1567 1302.2 0 1567 137 1528 1302.2 0 1528 138 1444 1302.2 0 1444 139 1291 1302.2 0 1291 140 2171 1302.2 0 2171 141 1080 1302.2 0 1080 142 990 1302.2 0 990 143 1507 1302.2 0 1507 144 2445 1302.2 0 2445 145 1652 1302.2 0 1652 146 1187 1302.2 0 1187 147 1313 1302.2 0 1313 148 1505 1302.2 0 1505 149 2355 1302.2 0 2355 150 3734 1302.2 0 3734 151 6883 1302.2 0 6883 152 1530 1302.2 0 1530 153 1799 1302.2 0 1799 154 1107 1302.2 0 1107 155 1184 1302.2 0 1184 156 3022 1302.2 0 3022 157 1764 1302.2 0 1764 158 1608 1302.2 0 1608 159 1205 1302.2 0 1205 160 1316 1302.2 0 1316 161 1648 1302.2 0 1648 162 1625 1302.2 0 1625 163 1526 1302.2 0 1526 164 2256 1302.2 0 2256 165 1551 1302.2 0 1551 166 1597 1302.2 0 1597 167 1666 1302.2 0 1666 168 1636 1302.2 0 1636 169 2383 1302.2 0 2383 170 1094 1302.2 0 1094 171 974 1302.2 0 974 172 1615 1302.2 0 1615 173 2087 1302.2 0 2087 174 709 1302.2 0 709 175 765 1302.2 0 765 176 1021 1302.2 0 1021 177 1483 1302.2 0 1483 178 2054 1302.2 0 2054 179 1798 1302.2 0 1798 180 1405 1302.2 0 1405 181 2986 1302.2 0 2986 182 1970 1302.2 0 1970 183 2958 1302.2 0 2958 184 2095 1302.2 0 2095 185 2062 1302.2 0 2062 186 1895 1302.2 0 1895 187 1195 1302.2 0 1195 188 1209 1302.2 0 1209 189 1698 1302.2 0 1698 190 2067 1302.2 0 2067 191 1701 1302.2 0 1701 192 1477 1302.2 0 1477 193 1640 1302.2 0 1640 194 1268 1302.2 0 1268 195 978 1302.2 0 978 196 1911 1302.2 0 1911 197 3994 1302.2 0 3994 198 1973 1302.2 0 1973 199 837 1302.2 0 837 200 634 1302.2 0 634 201 1501 1302.2 0 1501 202 2777 1302.2 0 2777 203 2174 1302.2 0 2174 204 1559 1302.2 0 1559 205 2842 1302.2 0 2842 206 1835 1302.2 0 1835 207 1237 1302.2 0 1237 208 1933 1302.2 0 1933 209 2291 1302.2 0 2291 210 2219 1302.2 0 2219 211 1671 1302.2 0 1671 212 1618 1302.2 0 1618 213 2166 1302.2 0 2166 214 2139 1302.2 0 2139 215 1705 1302.2 0 1705 216 2824 1302.2 0 2824 217 2245 1302.2 0 2245 218 3016 1302.2 0 3016 219 1856 1302.2 0 1856 220 1074 1302.2 0 1074 221 1517 1302.2 0 1517 222 2643 1302.2 0 2643 223 2529 1302.2 0 2529 224 2353 1302.2 0 2353 225 15992 1302.2 0 15992 226 6647 1302.2 0 6647 227 9683 1302.2 0 9683 228 1500 1302.2 0 1500 229 1665 1302.2 0 1665 230 2179 1302.2 0 2179 231 1773 1302.2 0 1773 232 1688 1302.2 0 1688 233 3251 1302.2 0 3251 234 4034 1302.2 0 4034 235 2564 1302.2 0 2564 236 2769 1302.2 0 2769 237 2569 1302.2 0 2569 238 2478 1302.2 0 2478 239 1972 1302.2 0 1972 240 1815 1302.2 0 1815 241 1897 1302.2 0 1897 242 2159 1302.2 0 2159 243 1844 1302.2 0 1844 244 1458 1302.2 0 1458 245 2008 1302.2 0 2008 246 1755 1302.2 0 1755 247 1712 1302.2 0 1712 248 1484 1302.2 0 1484 249 1229 1302.2 0 1229 250 1815 1302.2 0 1815 251 2561 1302.2 0 2561 252 1622 1302.2 0 1622 253 914 1302.2 0 914 254 885 1302.2 0 885 255 1158 1302.2 0 1158 256 1485 1302.2 0 1485 257 1105 1302.2 0 1105 258 1067 1302.2 0 1067 259 1537 1302.2 0 1537 260 2182 1302.2 0 2182 261 1854 1302.2 0 1854 262 1724 1302.2 0 1724 263 1842 1302.2 0 1842 264 1866 1302.2 0 1866 265 1296 1302.2 0 1296 266 2311 1302.2 0 2311 267 2255 1302.2 0 2255 268 1671 1302.2 0 1671 269 2109 1302.2 0 2109 270 3032 1302.2 0 3032 271 2500 1302.2 0 2500 272 2876 1302.2 0 2876 273 2728 1302.2 0 2728 274 2255 1302.2 0 2255 275 1552 1302.2 0 1552 276 1924 1302.2 0 1924 277 2486 1302.2 0 2486 278 2163 1302.2 0 2163 279 1889 1302.2 0 1889 280 1415 1302.2 0 1415 281 1451 1302.2 0 1451 282 1625 1302.2 0 1625 283 1563 1302.2 0 1563 284 2008 1302.2 0 2008 285 2029 1302.2 0 2029 286 1236 1302.2 0 1236 287 2110 1302.2 0 2110 288 2402 1302.2 0 2402 289 2443 1302.2 0 2443 290 3047 1302.2 0 3047 291 2904 1302.2 0 2904 292 3186 1302.2 0 3186 293 2263 1302.2 0 2263 294 1907 1302.2 0 1907 295 2494 1302.2 0 2494 296 3528 1302.2 0 3528 297 2197 1302.2 0 2197 298 1968 1302.2 0 1968 299 4737 1302.2 0 4737 300 3526 1302.2 0 3526 301 1491 1302.2 0 1491 Finished in 11.68 s (44 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 263,317 Reads with adapters: 8,229 (3.1%) Reads that were too short: 134 (0.1%) Reads written (passing filters): 8,095 (3.1%) Total basepairs processed: 44,778,063 bp Total written (filtered): 2,192,956 bp (4.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 8229 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 4 0.0 3 0 0 1 0 3 19 21 0.0 3 0 14 4 2 1 20 1628 0.0 4 1500 117 5 3 3 21 6296 0.0 4 6095 162 22 9 8 22 24 0.0 4 1 17 0 3 3 23 10 0.0 4 1 0 3 0 6 24 3 0.0 4 0 0 0 1 2 25 44 0.0 4 37 7 26 1 0.0 4 0 0 0 0 1 27 2 0.0 4 0 0 0 0 2 28 1 0.0 4 0 0 0 0 1 29 1 0.0 4 0 0 0 0 1 31 1 0.0 4 0 0 0 0 1 32 2 0.0 4 0 0 0 0 2 35 2 0.0 4 0 0 0 0 2 38 1 0.0 4 0 0 0 0 1 40 2 0.0 4 0 0 0 0 2 41 3 0.0 4 0 0 0 0 3 42 1 0.0 4 0 0 0 0 1 43 1 0.0 4 0 0 0 0 1 44 1 0.0 4 0 0 0 0 1 45 3 0.0 4 0 0 0 0 3 46 5 0.0 4 0 0 0 0 5 48 2 0.0 4 0 0 0 0 2 49 3 0.0 4 0 0 0 0 3 50 1 0.0 4 0 0 0 0 1 55 1 0.0 4 0 0 0 0 1 56 5 0.0 4 0 0 0 0 5 57 1 0.0 4 0 0 0 0 1 58 1 0.0 4 0 0 0 0 1 59 1 0.0 4 0 0 0 0 1 61 1 0.0 4 0 0 0 0 1 62 1 0.0 4 0 0 0 0 1 63 3 0.0 4 0 0 0 0 3 65 6 0.0 4 0 0 0 1 5 69 1 0.0 4 0 0 0 0 1 71 1 0.0 4 0 0 0 0 1 72 2 0.0 4 0 0 0 1 1 73 1 0.0 4 0 0 0 0 1 75 1 0.0 4 0 0 0 0 1 77 1 0.0 4 0 0 0 0 1 78 1 0.0 4 0 0 0 0 1 79 11 0.0 4 0 0 0 0 11 80 1 0.0 4 0 0 0 0 1 82 3 0.0 4 0 0 0 0 3 83 1 0.0 4 0 0 0 0 1 84 2 0.0 4 0 0 0 0 2 85 1 0.0 4 0 0 0 0 1 86 1 0.0 4 0 0 0 0 1 87 1 0.0 4 0 0 0 0 1 89 2 0.0 4 0 0 0 0 2 90 2 0.0 4 0 0 0 0 2 92 3 0.0 4 0 0 0 0 3 93 3 0.0 4 0 0 0 0 3 94 1 0.0 4 0 0 0 0 1 95 3 0.0 4 0 0 0 0 3 98 1 0.0 4 0 0 0 0 1 100 2 0.0 4 0 0 0 1 1 101 3 0.0 4 0 0 0 0 3 102 5 0.0 4 0 0 0 0 5 103 3 0.0 4 0 0 0 0 3 104 3 0.0 4 0 0 0 0 3 105 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 0 1 112 3 0.0 4 0 0 0 0 3 113 1 0.0 4 0 0 0 0 1 114 1 0.0 4 0 0 0 0 1 116 2 0.0 4 0 0 0 0 2 118 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 0 1 122 1 0.0 4 0 0 0 1 123 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 125 3 0.0 4 0 0 0 0 3 126 1 0.0 4 0 0 0 0 1 127 2 0.0 4 0 0 0 0 2 128 2 0.0 4 0 0 0 0 2 131 2 0.0 4 0 0 0 0 2 133 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 0 1 140 6 0.0 4 0 0 0 0 6 141 1 0.0 4 0 0 0 0 1 142 2 0.0 4 0 0 0 0 2 143 3 0.0 4 0 0 0 0 3 146 1 0.0 4 0 0 0 0 1 147 1 0.0 4 0 0 0 0 1 150 1 0.0 4 0 0 0 0 1 151 1 0.0 4 0 0 0 0 1 154 2 0.0 4 0 0 0 0 2 155 1 0.0 4 0 0 0 0 1 157 1 0.0 4 0 0 0 0 1 159 2 0.0 4 0 0 0 0 2 162 2 0.0 4 0 0 0 0 2 170 1 0.0 4 0 0 0 0 1 171 5 0.0 4 0 0 0 0 5 172 7 0.0 4 0 0 0 0 7 173 2 0.0 4 0 0 0 0 2 174 4 0.0 4 0 0 0 0 4 175 2 0.0 4 0 0 0 0 2 179 1 0.0 4 0 0 0 0 1 181 1 0.0 4 0 0 0 0 1 183 1 0.0 4 0 0 0 0 1 189 1 0.0 4 0 0 0 0 1 190 1 0.0 4 0 0 0 0 1 203 2 0.0 4 0 0 0 1 1 205 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.35 s (43 us/read; 1.39 M reads/minute). === Summary === Total reads processed: 8,095 Reads with adapters: 875 (10.8%) Reads written (passing filters): 8,095 (100.0%) Total basepairs processed: 2,192,956 bp Total written (filtered): 2,165,649 bp (98.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 875 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 4.0% C: 8.8% G: 84.0% T: 2.9% none/other: 0.3% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 9 124 0.0 0 10 114 10 108 0.0 1 2 106 11 561 0.0 1 3 558 12 2 0.0 1 0 2 150 1 0.0 1 1 215 33 0.0 1 27 6 216 1 0.0 1 1 217 1 0.0 1 1 218 1 0.0 1 1 220 1 0.0 1 1 232 1 0.0 1 1 242 7 0.0 1 7 256 19 0.0 1 18 1 257 2 0.0 1 2 258 2 0.0 1 2 266 1 0.0 1 1 267 1 0.0 1 1 268 1 0.0 1 0 1 272 5 0.0 1 5 273 3 0.0 1 3 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.