This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCTTGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 75.81 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 48,243 (1.6%) Reads that were too short: 9,034 (0.3%) Reads written (passing filters): 39,209 (1.3%) Total basepairs processed: 881,011,047 bp Total written (filtered): 9,053,113 bp (1.0%) === Adapter 1 === Sequence: TCTTGG; Type: regular 5'; Length: 6; Trimmed: 48243 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1011 714.6 0 1011 7 944 714.6 0 944 8 3465 714.6 0 3465 9 5588 714.6 0 5588 10 102 714.6 0 102 11 59 714.6 0 59 12 52 714.6 0 52 13 88 714.6 0 88 14 61 714.6 0 61 15 60 714.6 0 60 16 52 714.6 0 52 17 43 714.6 0 43 18 84 714.6 0 84 19 85 714.6 0 85 20 55 714.6 0 55 21 70 714.6 0 70 22 70 714.6 0 70 23 71 714.6 0 71 24 97 714.6 0 97 25 64 714.6 0 64 26 79 714.6 0 79 27 111 714.6 0 111 28 105 714.6 0 105 29 63 714.6 0 63 30 73 714.6 0 73 31 80 714.6 0 80 32 98 714.6 0 98 33 101 714.6 0 101 34 85 714.6 0 85 35 95 714.6 0 95 36 126 714.6 0 126 37 827 714.6 0 827 38 1348 714.6 0 1348 39 1642 714.6 0 1642 40 3618 714.6 0 3618 41 109 714.6 0 109 42 59 714.6 0 59 43 96 714.6 0 96 44 92 714.6 0 92 45 69 714.6 0 69 46 85 714.6 0 85 47 80 714.6 0 80 48 66 714.6 0 66 49 89 714.6 0 89 50 37 714.6 0 37 51 72 714.6 0 72 52 75 714.6 0 75 53 54 714.6 0 54 54 76 714.6 0 76 55 75 714.6 0 75 56 73 714.6 0 73 57 49 714.6 0 49 58 65 714.6 0 65 59 59 714.6 0 59 60 50 714.6 0 50 61 60 714.6 0 60 62 64 714.6 0 64 63 80 714.6 0 80 64 65 714.6 0 65 65 49 714.6 0 49 66 66 714.6 0 66 67 71 714.6 0 71 68 71 714.6 0 71 69 54 714.6 0 54 70 82 714.6 0 82 71 71 714.6 0 71 72 89 714.6 0 89 73 66 714.6 0 66 74 70 714.6 0 70 75 101 714.6 0 101 76 127 714.6 0 127 77 170 714.6 0 170 78 74 714.6 0 74 79 89 714.6 0 89 80 63 714.6 0 63 81 63 714.6 0 63 82 87 714.6 0 87 83 77 714.6 0 77 84 80 714.6 0 80 85 60 714.6 0 60 86 58 714.6 0 58 87 77 714.6 0 77 88 72 714.6 0 72 89 83 714.6 0 83 90 81 714.6 0 81 91 181 714.6 0 181 92 500 714.6 0 500 93 441 714.6 0 441 94 136 714.6 0 136 95 101 714.6 0 101 96 84 714.6 0 84 97 81 714.6 0 81 98 98 714.6 0 98 99 43 714.6 0 43 100 51 714.6 0 51 101 56 714.6 0 56 102 63 714.6 0 63 103 72 714.6 0 72 104 56 714.6 0 56 105 64 714.6 0 64 106 80 714.6 0 80 107 69 714.6 0 69 108 41 714.6 0 41 109 67 714.6 0 67 110 77 714.6 0 77 111 75 714.6 0 75 112 89 714.6 0 89 113 114 714.6 0 114 114 200 714.6 0 200 115 168 714.6 0 168 116 95 714.6 0 95 117 79 714.6 0 79 118 73 714.6 0 73 119 61 714.6 0 61 120 68 714.6 0 68 121 79 714.6 0 79 122 69 714.6 0 69 123 72 714.6 0 72 124 64 714.6 0 64 125 67 714.6 0 67 126 73 714.6 0 73 127 86 714.6 0 86 128 116 714.6 0 116 129 399 714.6 0 399 130 251 714.6 0 251 131 205 714.6 0 205 132 132 714.6 0 132 133 89 714.6 0 89 134 117 714.6 0 117 135 115 714.6 0 115 136 121 714.6 0 121 137 113 714.6 0 113 138 100 714.6 0 100 139 121 714.6 0 121 140 103 714.6 0 103 141 148 714.6 0 148 142 1293 714.6 0 1293 143 1796 714.6 0 1796 144 2029 714.6 0 2029 145 77 714.6 0 77 146 67 714.6 0 67 147 68 714.6 0 68 148 67 714.6 0 67 149 72 714.6 0 72 150 90 714.6 0 90 151 75 714.6 0 75 152 67 714.6 0 67 153 56 714.6 0 56 154 79 714.6 0 79 155 67 714.6 0 67 156 70 714.6 0 70 157 65 714.6 0 65 158 58 714.6 0 58 159 63 714.6 0 63 160 64 714.6 0 64 161 65 714.6 0 65 162 45 714.6 0 45 163 82 714.6 0 82 164 78 714.6 0 78 165 65 714.6 0 65 166 89 714.6 0 89 167 66 714.6 0 66 168 128 714.6 0 128 169 87 714.6 0 87 170 118 714.6 0 118 171 43 714.6 0 43 172 45 714.6 0 45 173 75 714.6 0 75 174 43 714.6 0 43 175 76 714.6 0 76 176 45 714.6 0 45 177 75 714.6 0 75 178 78 714.6 0 78 179 55 714.6 0 55 180 54 714.6 0 54 181 55 714.6 0 55 182 73 714.6 0 73 183 50 714.6 0 50 184 43 714.6 0 43 185 77 714.6 0 77 186 67 714.6 0 67 187 66 714.6 0 66 188 69 714.6 0 69 189 64 714.6 0 64 190 77 714.6 0 77 191 63 714.6 0 63 192 65 714.6 0 65 193 46 714.6 0 46 194 63 714.6 0 63 195 56 714.6 0 56 196 49 714.6 0 49 197 59 714.6 0 59 198 42 714.6 0 42 199 44 714.6 0 44 200 45 714.6 0 45 201 56 714.6 0 56 202 71 714.6 0 71 203 65 714.6 0 65 204 84 714.6 0 84 205 67 714.6 0 67 206 58 714.6 0 58 207 67 714.6 0 67 208 61 714.6 0 61 209 104 714.6 0 104 210 99 714.6 0 99 211 100 714.6 0 100 212 88 714.6 0 88 213 77 714.6 0 77 214 290 714.6 0 290 215 745 714.6 0 745 216 486 714.6 0 486 217 176 714.6 0 176 218 171 714.6 0 171 219 69 714.6 0 69 220 64 714.6 0 64 221 85 714.6 0 85 222 91 714.6 0 91 223 55 714.6 0 55 224 78 714.6 0 78 225 84 714.6 0 84 226 81 714.6 0 81 227 87 714.6 0 87 228 112 714.6 0 112 229 127 714.6 0 127 230 160 714.6 0 160 231 134 714.6 0 134 232 94 714.6 0 94 233 64 714.6 0 64 234 191 714.6 0 191 235 476 714.6 0 476 236 1010 714.6 0 1010 237 279 714.6 0 279 238 51 714.6 0 51 239 41 714.6 0 41 240 38 714.6 0 38 241 47 714.6 0 47 242 40 714.6 0 40 243 62 714.6 0 62 244 43 714.6 0 43 245 56 714.6 0 56 246 56 714.6 0 56 247 44 714.6 0 44 248 38 714.6 0 38 249 42 714.6 0 42 250 52 714.6 0 52 251 36 714.6 0 36 252 36 714.6 0 36 253 47 714.6 0 47 254 78 714.6 0 78 255 83 714.6 0 83 256 46 714.6 0 46 257 36 714.6 0 36 258 48 714.6 0 48 259 49 714.6 0 49 260 44 714.6 0 44 261 43 714.6 0 43 262 38 714.6 0 38 263 74 714.6 0 74 264 65 714.6 0 65 265 49 714.6 0 49 266 57 714.6 0 57 267 52 714.6 0 52 268 40 714.6 0 40 269 35 714.6 0 35 270 67 714.6 0 67 271 108 714.6 0 108 272 47 714.6 0 47 273 39 714.6 0 39 274 44 714.6 0 44 275 45 714.6 0 45 276 53 714.6 0 53 277 49 714.6 0 49 278 36 714.6 0 36 279 22 714.6 0 22 280 44 714.6 0 44 281 63 714.6 0 63 282 37 714.6 0 37 283 40 714.6 0 40 284 40 714.6 0 40 285 43 714.6 0 43 286 38 714.6 0 38 287 36 714.6 0 36 288 23 714.6 0 23 289 25 714.6 0 25 290 20 714.6 0 20 291 36 714.6 0 36 292 34 714.6 0 34 293 38 714.6 0 38 294 38 714.6 0 38 295 28 714.6 0 28 296 45 714.6 0 45 297 62 714.6 0 62 298 48 714.6 0 48 299 24 714.6 0 24 300 24 714.6 0 24 301 22 714.6 0 22 Finished in 1.81 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 39,209 Reads with adapters: 6,643 (16.9%) Reads that were too short: 7 (0.0%) Reads written (passing filters): 6,636 (16.9%) Total basepairs processed: 9,053,113 bp Total written (filtered): 1,803,922 bp (19.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 6643 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 4 0.0 3 0 0 3 0 1 19 46 0.0 3 0 37 6 2 1 20 5638 0.0 4 5271 319 36 7 5 21 738 0.0 4 693 29 9 5 2 22 11 0.0 4 3 7 1 23 174 0.0 4 159 7 2 2 4 24 2 0.0 4 0 1 0 0 1 25 2 0.0 4 0 0 0 0 2 27 1 0.0 4 1 57 1 0.0 4 0 0 0 0 1 63 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 0 0 0 1 97 4 0.0 4 0 0 0 0 4 107 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 0 0 0 1 111 2 0.0 4 0 0 0 2 112 1 0.0 4 0 0 0 1 124 1 0.0 4 0 0 0 1 127 6 0.0 4 0 0 0 0 6 153 1 0.0 4 0 0 0 0 1 160 1 0.0 4 0 0 0 0 1 165 1 0.0 4 0 0 0 0 1 166 1 0.0 4 0 0 0 0 1 206 1 0.0 4 0 0 0 0 1 221 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.23 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 6,636 Reads with adapters: 697 (10.5%) Reads written (passing filters): 6,636 (100.0%) Total basepairs processed: 1,803,922 bp Total written (filtered): 1,700,601 bp (94.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 697 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 55.8% C: 3.2% G: 40.6% T: 0.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 103.7 0 1 5 1 6.5 0 1 9 38 0.0 0 7 31 10 204 0.0 1 9 195 11 79 0.0 1 0 79 12 1 0.0 1 0 1 13 3 0.0 1 0 3 150 2 0.0 1 2 259 1 0.0 1 1 260 1 0.0 1 1 270 3 0.0 1 3 271 355 0.0 1 345 10 272 7 0.0 1 7 273 1 0.0 1 1