This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTCTCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 73.09 s (25 us/read; 2.40 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 61,052 (2.1%) Reads that were too short: 6,410 (0.2%) Reads written (passing filters): 54,642 (1.9%) Total basepairs processed: 881,011,047 bp Total written (filtered): 12,012,786 bp (1.4%) === Adapter 1 === Sequence: TTCTCG; Type: regular 5'; Length: 6; Trimmed: 61052 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 284 714.6 0 284 7 206 714.6 0 206 8 369 714.6 0 369 9 13452 714.6 0 13452 10 81 714.6 0 81 11 54 714.6 0 54 12 67 714.6 0 67 13 66 714.6 0 66 14 56 714.6 0 56 15 71 714.6 0 71 16 53 714.6 0 53 17 78 714.6 0 78 18 84 714.6 0 84 19 60 714.6 0 60 20 63 714.6 0 63 21 55 714.6 0 55 22 56 714.6 0 56 23 54 714.6 0 54 24 56 714.6 0 56 25 63 714.6 0 63 26 51 714.6 0 51 27 82 714.6 0 82 28 56 714.6 0 56 29 50 714.6 0 50 30 63 714.6 0 63 31 57 714.6 0 57 32 67 714.6 0 67 33 85 714.6 0 85 34 71 714.6 0 71 35 55 714.6 0 55 36 60 714.6 0 60 37 100 714.6 0 100 38 75 714.6 0 75 39 2604 714.6 0 2604 40 1118 714.6 0 1118 41 69 714.6 0 69 42 37 714.6 0 37 43 49 714.6 0 49 44 56 714.6 0 56 45 38 714.6 0 38 46 72 714.6 0 72 47 57 714.6 0 57 48 66 714.6 0 66 49 100 714.6 0 100 50 45 714.6 0 45 51 60 714.6 0 60 52 82 714.6 0 82 53 70 714.6 0 70 54 70 714.6 0 70 55 40 714.6 0 40 56 47 714.6 0 47 57 63 714.6 0 63 58 68 714.6 0 68 59 47 714.6 0 47 60 47 714.6 0 47 61 44 714.6 0 44 62 49 714.6 0 49 63 41 714.6 0 41 64 42 714.6 0 42 65 36 714.6 0 36 66 31 714.6 0 31 67 39 714.6 0 39 68 40 714.6 0 40 69 44 714.6 0 44 70 56 714.6 0 56 71 51 714.6 0 51 72 44 714.6 0 44 73 56 714.6 0 56 74 83 714.6 0 83 75 74 714.6 0 74 76 138 714.6 0 138 77 81 714.6 0 81 78 50 714.6 0 50 79 58 714.6 0 58 80 49 714.6 0 49 81 73 714.6 0 73 82 85 714.6 0 85 83 78 714.6 0 78 84 77 714.6 0 77 85 75 714.6 0 75 86 79 714.6 0 79 87 54 714.6 0 54 88 63 714.6 0 63 89 77 714.6 0 77 90 237 714.6 0 237 91 506 714.6 0 506 92 449 714.6 0 449 93 113 714.6 0 113 94 176 714.6 0 176 95 148 714.6 0 148 96 144 714.6 0 144 97 152 714.6 0 152 98 232 714.6 0 232 99 95 714.6 0 95 100 79 714.6 0 79 101 140 714.6 0 140 102 104 714.6 0 104 103 87 714.6 0 87 104 155 714.6 0 155 105 102 714.6 0 102 106 109 714.6 0 109 107 147 714.6 0 147 108 175 714.6 0 175 109 168 714.6 0 168 110 366 714.6 0 366 111 995 714.6 0 995 112 17520 714.6 0 17520 113 643 714.6 0 643 114 98 714.6 0 98 115 97 714.6 0 97 116 65 714.6 0 65 117 73 714.6 0 73 118 81 714.6 0 81 119 49 714.6 0 49 120 52 714.6 0 52 121 41 714.6 0 41 122 33 714.6 0 33 123 55 714.6 0 55 124 67 714.6 0 67 125 53 714.6 0 53 126 45 714.6 0 45 127 51 714.6 0 51 128 58 714.6 0 58 129 94 714.6 0 94 130 91 714.6 0 91 131 95 714.6 0 95 132 93 714.6 0 93 133 89 714.6 0 89 134 52 714.6 0 52 135 91 714.6 0 91 136 59 714.6 0 59 137 87 714.6 0 87 138 54 714.6 0 54 139 67 714.6 0 67 140 66 714.6 0 66 141 91 714.6 0 91 142 71 714.6 0 71 143 206 714.6 0 206 144 198 714.6 0 198 145 137 714.6 0 137 146 72 714.6 0 72 147 68 714.6 0 68 148 94 714.6 0 94 149 112 714.6 0 112 150 95 714.6 0 95 151 68 714.6 0 68 152 97 714.6 0 97 153 98 714.6 0 98 154 185 714.6 0 185 155 66 714.6 0 66 156 98 714.6 0 98 157 82 714.6 0 82 158 69 714.6 0 69 159 94 714.6 0 94 160 81 714.6 0 81 161 279 714.6 0 279 162 467 714.6 0 467 163 406 714.6 0 406 164 326 714.6 0 326 165 97 714.6 0 97 166 51 714.6 0 51 167 64 714.6 0 64 168 84 714.6 0 84 169 101 714.6 0 101 170 87 714.6 0 87 171 104 714.6 0 104 172 181 714.6 0 181 173 52 714.6 0 52 174 64 714.6 0 64 175 35 714.6 0 35 176 105 714.6 0 105 177 244 714.6 0 244 178 176 714.6 0 176 179 188 714.6 0 188 180 66 714.6 0 66 181 67 714.6 0 67 182 64 714.6 0 64 183 54 714.6 0 54 184 65 714.6 0 65 185 68 714.6 0 68 186 56 714.6 0 56 187 43 714.6 0 43 188 94 714.6 0 94 189 108 714.6 0 108 190 177 714.6 0 177 191 155 714.6 0 155 192 59 714.6 0 59 193 48 714.6 0 48 194 51 714.6 0 51 195 55 714.6 0 55 196 103 714.6 0 103 197 91 714.6 0 91 198 62 714.6 0 62 199 80 714.6 0 80 200 56 714.6 0 56 201 57 714.6 0 57 202 47 714.6 0 47 203 53 714.6 0 53 204 95 714.6 0 95 205 88 714.6 0 88 206 60 714.6 0 60 207 56 714.6 0 56 208 59 714.6 0 59 209 45 714.6 0 45 210 57 714.6 0 57 211 132 714.6 0 132 212 120 714.6 0 120 213 97 714.6 0 97 214 85 714.6 0 85 215 118 714.6 0 118 216 112 714.6 0 112 217 110 714.6 0 110 218 76 714.6 0 76 219 69 714.6 0 69 220 49 714.6 0 49 221 55 714.6 0 55 222 55 714.6 0 55 223 77 714.6 0 77 224 137 714.6 0 137 225 274 714.6 0 274 226 114 714.6 0 114 227 66 714.6 0 66 228 60 714.6 0 60 229 63 714.6 0 63 230 48 714.6 0 48 231 68 714.6 0 68 232 65 714.6 0 65 233 77 714.6 0 77 234 74 714.6 0 74 235 87 714.6 0 87 236 88 714.6 0 88 237 74 714.6 0 74 238 71 714.6 0 71 239 51 714.6 0 51 240 33 714.6 0 33 241 45 714.6 0 45 242 66 714.6 0 66 243 105 714.6 0 105 244 182 714.6 0 182 245 164 714.6 0 164 246 161 714.6 0 161 247 68 714.6 0 68 248 43 714.6 0 43 249 42 714.6 0 42 250 32 714.6 0 32 251 50 714.6 0 50 252 47 714.6 0 47 253 57 714.6 0 57 254 87 714.6 0 87 255 80 714.6 0 80 256 58 714.6 0 58 257 47 714.6 0 47 258 35 714.6 0 35 259 58 714.6 0 58 260 45 714.6 0 45 261 44 714.6 0 44 262 47 714.6 0 47 263 54 714.6 0 54 264 72 714.6 0 72 265 95 714.6 0 95 266 62 714.6 0 62 267 43 714.6 0 43 268 28 714.6 0 28 269 39 714.6 0 39 270 41 714.6 0 41 271 44 714.6 0 44 272 50 714.6 0 50 273 30 714.6 0 30 274 35 714.6 0 35 275 35 714.6 0 35 276 56 714.6 0 56 277 51 714.6 0 51 278 41 714.6 0 41 279 37 714.6 0 37 280 39 714.6 0 39 281 27 714.6 0 27 282 38 714.6 0 38 283 38 714.6 0 38 284 48 714.6 0 48 285 58 714.6 0 58 286 79 714.6 0 79 287 39 714.6 0 39 288 36 714.6 0 36 289 27 714.6 0 27 290 40 714.6 0 40 291 50 714.6 0 50 292 28 714.6 0 28 293 38 714.6 0 38 294 32 714.6 0 32 295 28 714.6 0 28 296 42 714.6 0 42 297 74 714.6 0 74 298 32 714.6 0 32 299 33 714.6 0 33 300 27 714.6 0 27 301 16 714.6 0 16 Finished in 2.40 s (44 us/read; 1.37 M reads/minute). === Summary === Total reads processed: 54,642 Reads with adapters: 14,033 (25.7%) Reads that were too short: 10 (0.0%) Reads written (passing filters): 14,023 (25.7%) Total basepairs processed: 12,012,786 bp Total written (filtered): 3,814,075 bp (31.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 14033 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 8 0.0 3 0 0 7 1 19 113 0.0 3 0 106 2 5 20 13719 0.0 4 12730 844 109 19 17 21 56 0.0 4 12 39 1 3 1 22 5 0.0 4 0 4 1 23 111 0.0 4 88 9 3 10 1 24 1 0.0 4 1 27 1 0.0 4 0 0 0 0 1 32 1 0.0 4 0 0 0 0 1 39 1 0.0 4 0 0 0 0 1 56 5 0.0 4 0 0 0 0 5 58 1 0.0 4 0 0 0 0 1 59 1 0.0 4 0 0 0 0 1 76 1 0.0 4 0 0 0 0 1 87 2 0.0 4 0 0 0 1 1 108 1 0.0 4 0 0 0 1 111 1 0.0 4 0 0 0 0 1 168 1 0.0 4 0 0 0 0 1 172 1 0.0 4 0 0 0 0 1 186 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.48 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 14,023 Reads with adapters: 802 (5.7%) Reads written (passing filters): 14,023 (100.0%) Total basepairs processed: 3,814,075 bp Total written (filtered): 3,805,361 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 802 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 7.6% C: 5.2% G: 86.0% T: 1.1% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 4 1 54.8 0 1 9 137 0.1 0 10 127 10 585 0.0 1 25 560 11 71 0.0 1 0 71 12 2 0.0 1 0 2 13 3 0.0 1 0 3 243 1 0.0 1 1 270 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.