This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGCGCA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 69.86 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 268,886 (9.9%) Reads that were too short: 6,961 (0.3%) Reads written (passing filters): 261,925 (9.7%) Total basepairs processed: 815,312,680 bp Total written (filtered): 63,367,919 bp (7.8%) === Adapter 1 === Sequence: GGCGCA; Type: regular 5'; Length: 6; Trimmed: 268886 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 141 661.3 0 141 7 1055 661.3 0 1055 8 153 661.3 0 153 9 208 661.3 0 208 10 288 661.3 0 288 11 180 661.3 0 180 12 149 661.3 0 149 13 342 661.3 0 342 14 424 661.3 0 424 15 442 661.3 0 442 16 250 661.3 0 250 17 306 661.3 0 306 18 377 661.3 0 377 19 538 661.3 0 538 20 8840 661.3 0 8840 21 20047 661.3 0 20047 22 30616 661.3 0 30616 23 191 661.3 0 191 24 149 661.3 0 149 25 130 661.3 0 130 26 102 661.3 0 102 27 74 661.3 0 74 28 85 661.3 0 85 29 96 661.3 0 96 30 73 661.3 0 73 31 165 661.3 0 165 32 210 661.3 0 210 33 375 661.3 0 375 34 335 661.3 0 335 35 687 661.3 0 687 36 1561 661.3 0 1561 37 362 661.3 0 362 38 558 661.3 0 558 39 1084 661.3 0 1084 40 604 661.3 0 604 41 502 661.3 0 502 42 765 661.3 0 765 43 766 661.3 0 766 44 1046 661.3 0 1046 45 782 661.3 0 782 46 845 661.3 0 845 47 1046 661.3 0 1046 48 1689 661.3 0 1689 49 29668 661.3 0 29668 50 49202 661.3 0 49202 51 68876 661.3 0 68876 52 369 661.3 0 369 53 47 661.3 0 47 54 78 661.3 0 78 55 39 661.3 0 39 56 93 661.3 0 93 57 113 661.3 0 113 58 53 661.3 0 53 59 50 661.3 0 50 60 55 661.3 0 55 61 50 661.3 0 50 62 103 661.3 0 103 63 83 661.3 0 83 64 252 661.3 0 252 65 321 661.3 0 321 66 32 661.3 0 32 67 44 661.3 0 44 68 43 661.3 0 43 69 42 661.3 0 42 70 48 661.3 0 48 71 45 661.3 0 45 72 36 661.3 0 36 73 52 661.3 0 52 74 54 661.3 0 54 75 45 661.3 0 45 76 61 661.3 0 61 77 39 661.3 0 39 78 39 661.3 0 39 79 40 661.3 0 40 80 151 661.3 0 151 81 40 661.3 0 40 82 36 661.3 0 36 83 47 661.3 0 47 84 72 661.3 0 72 85 63 661.3 0 63 86 71 661.3 0 71 87 40 661.3 0 40 88 60 661.3 0 60 89 40 661.3 0 40 90 36 661.3 0 36 91 26 661.3 0 26 92 36 661.3 0 36 93 58 661.3 0 58 94 35 661.3 0 35 95 35 661.3 0 35 96 32 661.3 0 32 97 40 661.3 0 40 98 67 661.3 0 67 99 40 661.3 0 40 100 24 661.3 0 24 101 42 661.3 0 42 102 44 661.3 0 44 103 49 661.3 0 49 104 39 661.3 0 39 105 37 661.3 0 37 106 49 661.3 0 49 107 35 661.3 0 35 108 46 661.3 0 46 109 35 661.3 0 35 110 37 661.3 0 37 111 32 661.3 0 32 112 41 661.3 0 41 113 33 661.3 0 33 114 35 661.3 0 35 115 36 661.3 0 36 116 24 661.3 0 24 117 128 661.3 0 128 118 76 661.3 0 76 119 120 661.3 0 120 120 57 661.3 0 57 121 48 661.3 0 48 122 49 661.3 0 49 123 40 661.3 0 40 124 97 661.3 0 97 125 49 661.3 0 49 126 54 661.3 0 54 127 55 661.3 0 55 128 38 661.3 0 38 129 38 661.3 0 38 130 56 661.3 0 56 131 67 661.3 0 67 132 32 661.3 0 32 133 41 661.3 0 41 134 46 661.3 0 46 135 42 661.3 0 42 136 51 661.3 0 51 137 38 661.3 0 38 138 50 661.3 0 50 139 40 661.3 0 40 140 57 661.3 0 57 141 51 661.3 0 51 142 29 661.3 0 29 143 58 661.3 0 58 144 76 661.3 0 76 145 29 661.3 0 29 146 64 661.3 0 64 147 38 661.3 0 38 148 41 661.3 0 41 149 39 661.3 0 39 150 50 661.3 0 50 151 46 661.3 0 46 152 44 661.3 0 44 153 37 661.3 0 37 154 34 661.3 0 34 155 53 661.3 0 53 156 44 661.3 0 44 157 90 661.3 0 90 158 45 661.3 0 45 159 47 661.3 0 47 160 43 661.3 0 43 161 52 661.3 0 52 162 37 661.3 0 37 163 39 661.3 0 39 164 51 661.3 0 51 165 50 661.3 0 50 166 45 661.3 0 45 167 57 661.3 0 57 168 37 661.3 0 37 169 51 661.3 0 51 170 86 661.3 0 86 171 67 661.3 0 67 172 62 661.3 0 62 173 56 661.3 0 56 174 53 661.3 0 53 175 49 661.3 0 49 176 78 661.3 0 78 177 93 661.3 0 93 178 85 661.3 0 85 179 166 661.3 0 166 180 271 661.3 0 271 181 914 661.3 0 914 182 9635 661.3 0 9635 183 681 661.3 0 681 184 1652 661.3 0 1652 185 131 661.3 0 131 186 98 661.3 0 98 187 80 661.3 0 80 188 132 661.3 0 132 189 98 661.3 0 98 190 120 661.3 0 120 191 98 661.3 0 98 192 103 661.3 0 103 193 483 661.3 0 483 194 779 661.3 0 779 195 2355 661.3 0 2355 196 2380 661.3 0 2380 197 7731 661.3 0 7731 198 101 661.3 0 101 199 57 661.3 0 57 200 64 661.3 0 64 201 48 661.3 0 48 202 37 661.3 0 37 203 38 661.3 0 38 204 34 661.3 0 34 205 37 661.3 0 37 206 34 661.3 0 34 207 32 661.3 0 32 208 47 661.3 0 47 209 30 661.3 0 30 210 31 661.3 0 31 211 42 661.3 0 42 212 41 661.3 0 41 213 95 661.3 0 95 214 74 661.3 0 74 215 90 661.3 0 90 216 69 661.3 0 69 217 59 661.3 0 59 218 69 661.3 0 69 219 78 661.3 0 78 220 108 661.3 0 108 221 100 661.3 0 100 222 205 661.3 0 205 223 45 661.3 0 45 224 39 661.3 0 39 225 53 661.3 0 53 226 35 661.3 0 35 227 38 661.3 0 38 228 48 661.3 0 48 229 41 661.3 0 41 230 39 661.3 0 39 231 49 661.3 0 49 232 46 661.3 0 46 233 51 661.3 0 51 234 81 661.3 0 81 235 64 661.3 0 64 236 61 661.3 0 61 237 49 661.3 0 49 238 47 661.3 0 47 239 87 661.3 0 87 240 129 661.3 0 129 241 212 661.3 0 212 242 75 661.3 0 75 243 41 661.3 0 41 244 56 661.3 0 56 245 47 661.3 0 47 246 60 661.3 0 60 247 54 661.3 0 54 248 66 661.3 0 66 249 60 661.3 0 60 250 102 661.3 0 102 251 133 661.3 0 133 252 197 661.3 0 197 253 100 661.3 0 100 254 36 661.3 0 36 255 57 661.3 0 57 256 52 661.3 0 52 257 68 661.3 0 68 258 71 661.3 0 71 259 45 661.3 0 45 260 60 661.3 0 60 261 52 661.3 0 52 262 97 661.3 0 97 263 88 661.3 0 88 264 175 661.3 0 175 265 116 661.3 0 116 266 78 661.3 0 78 267 77 661.3 0 77 268 52 661.3 0 52 269 41 661.3 0 41 270 39 661.3 0 39 271 43 661.3 0 43 272 48 661.3 0 48 273 43 661.3 0 43 274 47 661.3 0 47 275 58 661.3 0 58 276 38 661.3 0 38 277 35 661.3 0 35 278 55 661.3 0 55 279 51 661.3 0 51 280 47 661.3 0 47 281 45 661.3 0 45 282 43 661.3 0 43 283 49 661.3 0 49 284 45 661.3 0 45 285 76 661.3 0 76 286 53 661.3 0 53 287 44 661.3 0 44 288 104 661.3 0 104 289 302 661.3 0 302 290 399 661.3 0 399 291 61 661.3 0 61 292 48 661.3 0 48 293 87 661.3 0 87 294 55 661.3 0 55 295 35 661.3 0 35 296 27 661.3 0 27 297 36 661.3 0 36 298 32 661.3 0 32 299 31 661.3 0 31 300 78 661.3 0 78 301 87 661.3 0 87 Finished in 10.06 s (38 us/read; 1.56 M reads/minute). === Summary === Total reads processed: 261,925 Reads with adapters: 1,557 (0.6%) Reads that were too short: 349 (0.1%) Reads written (passing filters): 1,208 (0.5%) Total basepairs processed: 63,367,919 bp Total written (filtered): 269,823 bp (0.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 1557 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 8 0.0 3 0 8 17 750 0.0 3 740 10 18 7 0.0 3 1 2 2 2 19 3 0.0 3 1 0 1 1 25 1 0.0 3 0 0 0 1 29 6 0.0 3 4 1 0 1 32 1 0.0 3 0 0 0 1 34 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 46 3 0.0 3 0 0 0 3 49 3 0.0 3 0 0 0 3 50 1 0.0 3 0 0 0 1 51 2 0.0 3 0 0 0 2 52 1 0.0 3 0 0 0 1 54 2 0.0 3 0 0 0 2 67 2 0.0 3 0 0 0 2 69 1 0.0 3 0 0 0 1 73 15 0.0 3 0 0 0 15 74 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 90 3 0.0 3 0 0 0 3 91 2 0.0 3 0 0 2 92 4 0.0 3 0 0 0 4 93 7 0.0 3 0 0 2 5 94 2 0.0 3 0 0 0 2 97 5 0.0 3 0 0 0 5 98 2 0.0 3 0 0 0 2 99 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 102 2 0.0 3 0 0 0 2 103 1 0.0 3 0 0 0 1 104 3 0.0 3 0 0 0 3 106 1 0.0 3 0 0 0 1 107 2 0.0 3 0 0 1 1 112 1 0.0 3 0 0 0 1 113 1 0.0 3 1 114 1 0.0 3 0 0 0 1 117 2 0.0 3 0 0 0 2 118 2 0.0 3 0 0 0 2 121 1 0.0 3 0 0 0 1 122 62 0.0 3 0 0 0 62 123 47 0.0 3 0 0 0 47 124 31 0.0 3 0 0 0 31 125 14 0.0 3 0 0 0 14 126 6 0.0 3 0 0 0 6 127 5 0.0 3 0 0 0 5 128 24 0.0 3 0 0 0 24 130 2 0.0 3 0 0 0 2 131 2 0.0 3 0 0 0 2 133 1 0.0 3 0 0 0 1 134 3 0.0 3 0 0 0 3 135 2 0.0 3 0 0 0 2 137 11 0.0 3 0 0 0 11 138 10 0.0 3 0 0 0 10 139 8 0.0 3 0 0 0 8 140 1 0.0 3 0 0 0 1 142 26 0.0 3 0 0 0 26 143 2 0.0 3 0 0 0 2 145 4 0.0 3 0 0 0 4 147 1 0.0 3 0 0 0 1 148 2 0.0 3 0 0 0 2 149 2 0.0 3 0 0 0 2 151 15 0.0 3 0 0 0 15 152 3 0.0 3 0 0 0 3 153 8 0.0 3 0 0 0 8 154 2 0.0 3 0 0 0 2 156 1 0.0 3 0 0 0 1 157 2 0.0 3 0 0 0 2 159 2 0.0 3 0 0 0 2 160 3 0.0 3 3 165 3 0.0 3 0 0 0 3 166 2 0.0 3 0 0 0 2 167 1 0.0 3 0 0 0 1 168 3 0.0 3 0 0 0 3 169 1 0.0 3 0 0 0 1 170 3 0.0 3 0 0 0 3 171 26 0.0 3 0 0 0 26 172 23 0.0 3 0 0 0 23 174 9 0.0 3 0 0 0 9 175 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 197 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 209 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 229 2 0.0 3 0 0 0 2 234 83 0.0 3 0 0 0 83 235 13 0.0 3 0 0 0 13 236 14 0.0 3 0 0 0 14 237 41 0.0 3 0 0 0 41 238 84 0.0 3 0 0 2 82 239 11 0.0 3 0 0 0 11 240 12 0.0 3 0 0 0 12 242 3 0.0 3 0 0 0 3 243 6 0.0 3 0 0 0 6 244 12 0.0 3 0 1 1 10 245 1 0.0 3 0 0 0 1 249 1 0.0 3 0 0 0 1 250 7 0.0 3 0 0 0 7 251 4 0.0 3 0 0 0 4 262 1 0.0 3 0 0 0 1 263 6 0.0 3 0 0 0 6 264 1 0.0 3 0 0 0 1 265 2 0.0 3 0 0 0 2 266 5 0.0 3 0 0 0 5 267 2 0.0 3 0 0 0 2 270 1 0.0 3 0 0 0 1 272 1 0.0 3 0 0 0 1 273 1 0.0 3 0 0 0 1 276 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.04 s (33 us/read; 1.81 M reads/minute). === Summary === Total reads processed: 1,208 Reads with adapters: 35 (2.9%) Reads written (passing filters): 1,208 (100.0%) Total basepairs processed: 269,823 bp Total written (filtered): 269,718 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 35 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 57.1% C: 17.1% G: 20.0% T: 5.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 35 18.9 0 35