This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGTCAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 80.09 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 34,535 (1.1%) Reads that were too short: 10,293 (0.3%) Reads written (passing filters): 24,242 (0.8%) Total basepairs processed: 905,907,359 bp Total written (filtered): 5,859,812 bp (0.6%) === Adapter 1 === Sequence: CGTCAC; Type: regular 5'; Length: 6; Trimmed: 34535 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 387 734.8 0 387 7 1836 734.8 0 1836 8 10806 734.8 0 10806 9 56 734.8 0 56 10 11 734.8 0 11 11 23 734.8 0 23 12 16 734.8 0 16 13 27 734.8 0 27 14 23 734.8 0 23 15 22 734.8 0 22 16 24 734.8 0 24 17 21 734.8 0 21 18 38 734.8 0 38 19 22 734.8 0 22 20 27 734.8 0 27 21 21 734.8 0 21 22 24 734.8 0 24 23 34 734.8 0 34 24 22 734.8 0 22 25 23 734.8 0 23 26 34 734.8 0 34 27 20 734.8 0 20 28 38 734.8 0 38 29 26 734.8 0 26 30 49 734.8 0 49 31 228 734.8 0 228 32 572 734.8 0 572 33 41 734.8 0 41 34 23 734.8 0 23 35 29 734.8 0 29 36 33 734.8 0 33 37 40 734.8 0 40 38 21 734.8 0 21 39 20 734.8 0 20 40 38 734.8 0 38 41 27 734.8 0 27 42 17 734.8 0 17 43 54 734.8 0 54 44 36 734.8 0 36 45 21 734.8 0 21 46 30 734.8 0 30 47 36 734.8 0 36 48 22 734.8 0 22 49 28 734.8 0 28 50 13 734.8 0 13 51 21 734.8 0 21 52 28 734.8 0 28 53 32 734.8 0 32 54 38 734.8 0 38 55 32 734.8 0 32 56 12 734.8 0 12 57 14 734.8 0 14 58 29 734.8 0 29 59 32 734.8 0 32 60 25 734.8 0 25 61 25 734.8 0 25 62 35 734.8 0 35 63 27 734.8 0 27 64 140 734.8 0 140 65 378 734.8 0 378 66 70 734.8 0 70 67 20 734.8 0 20 68 33 734.8 0 33 69 71 734.8 0 71 70 30 734.8 0 30 71 25 734.8 0 25 72 12 734.8 0 12 73 11 734.8 0 11 74 13 734.8 0 13 75 20 734.8 0 20 76 20 734.8 0 20 77 21 734.8 0 21 78 16 734.8 0 16 79 17 734.8 0 17 80 27 734.8 0 27 81 46 734.8 0 46 82 25 734.8 0 25 83 47 734.8 0 47 84 44 734.8 0 44 85 34 734.8 0 34 86 24 734.8 0 24 87 18 734.8 0 18 88 31 734.8 0 31 89 27 734.8 0 27 90 33 734.8 0 33 91 29 734.8 0 29 92 209 734.8 0 209 93 144 734.8 0 144 94 55 734.8 0 55 95 80 734.8 0 80 96 44 734.8 0 44 97 27 734.8 0 27 98 25 734.8 0 25 99 32 734.8 0 32 100 55 734.8 0 55 101 52 734.8 0 52 102 35 734.8 0 35 103 31 734.8 0 31 104 32 734.8 0 32 105 28 734.8 0 28 106 25 734.8 0 25 107 23 734.8 0 23 108 19 734.8 0 19 109 30 734.8 0 30 110 38 734.8 0 38 111 38 734.8 0 38 112 22 734.8 0 22 113 29 734.8 0 29 114 37 734.8 0 37 115 43 734.8 0 43 116 42 734.8 0 42 117 74 734.8 0 74 118 47 734.8 0 47 119 30 734.8 0 30 120 41 734.8 0 41 121 46 734.8 0 46 122 57 734.8 0 57 123 62 734.8 0 62 124 41 734.8 0 41 125 49 734.8 0 49 126 36 734.8 0 36 127 45 734.8 0 45 128 48 734.8 0 48 129 46 734.8 0 46 130 69 734.8 0 69 131 71 734.8 0 71 132 68 734.8 0 68 133 39 734.8 0 39 134 48 734.8 0 48 135 59 734.8 0 59 136 89 734.8 0 89 137 95 734.8 0 95 138 66 734.8 0 66 139 49 734.8 0 49 140 49 734.8 0 49 141 58 734.8 0 58 142 44 734.8 0 44 143 65 734.8 0 65 144 88 734.8 0 88 145 51 734.8 0 51 146 50 734.8 0 50 147 144 734.8 0 144 148 111 734.8 0 111 149 97 734.8 0 97 150 97 734.8 0 97 151 59 734.8 0 59 152 65 734.8 0 65 153 124 734.8 0 124 154 50 734.8 0 50 155 84 734.8 0 84 156 126 734.8 0 126 157 135 734.8 0 135 158 119 734.8 0 119 159 97 734.8 0 97 160 99 734.8 0 99 161 95 734.8 0 95 162 84 734.8 0 84 163 61 734.8 0 61 164 60 734.8 0 60 165 42 734.8 0 42 166 66 734.8 0 66 167 114 734.8 0 114 168 131 734.8 0 131 169 77 734.8 0 77 170 76 734.8 0 76 171 92 734.8 0 92 172 84 734.8 0 84 173 86 734.8 0 86 174 52 734.8 0 52 175 138 734.8 0 138 176 299 734.8 0 299 177 147 734.8 0 147 178 71 734.8 0 71 179 69 734.8 0 69 180 37 734.8 0 37 181 63 734.8 0 63 182 69 734.8 0 69 183 77 734.8 0 77 184 89 734.8 0 89 185 58 734.8 0 58 186 108 734.8 0 108 187 108 734.8 0 108 188 162 734.8 0 162 189 96 734.8 0 96 190 65 734.8 0 65 191 56 734.8 0 56 192 44 734.8 0 44 193 56 734.8 0 56 194 54 734.8 0 54 195 62 734.8 0 62 196 53 734.8 0 53 197 57 734.8 0 57 198 35 734.8 0 35 199 71 734.8 0 71 200 64 734.8 0 64 201 41 734.8 0 41 202 43 734.8 0 43 203 49 734.8 0 49 204 65 734.8 0 65 205 57 734.8 0 57 206 62 734.8 0 62 207 52 734.8 0 52 208 60 734.8 0 60 209 53 734.8 0 53 210 70 734.8 0 70 211 57 734.8 0 57 212 84 734.8 0 84 213 73 734.8 0 73 214 109 734.8 0 109 215 116 734.8 0 116 216 132 734.8 0 132 217 108 734.8 0 108 218 89 734.8 0 89 219 91 734.8 0 91 220 85 734.8 0 85 221 127 734.8 0 127 222 143 734.8 0 143 223 214 734.8 0 214 224 105 734.8 0 105 225 124 734.8 0 124 226 93 734.8 0 93 227 135 734.8 0 135 228 97 734.8 0 97 229 105 734.8 0 105 230 76 734.8 0 76 231 62 734.8 0 62 232 66 734.8 0 66 233 105 734.8 0 105 234 63 734.8 0 63 235 93 734.8 0 93 236 99 734.8 0 99 237 92 734.8 0 92 238 116 734.8 0 116 239 106 734.8 0 106 240 85 734.8 0 85 241 147 734.8 0 147 242 123 734.8 0 123 243 182 734.8 0 182 244 107 734.8 0 107 245 123 734.8 0 123 246 75 734.8 0 75 247 100 734.8 0 100 248 162 734.8 0 162 249 144 734.8 0 144 250 49 734.8 0 49 251 44 734.8 0 44 252 59 734.8 0 59 253 105 734.8 0 105 254 166 734.8 0 166 255 160 734.8 0 160 256 131 734.8 0 131 257 97 734.8 0 97 258 82 734.8 0 82 259 77 734.8 0 77 260 88 734.8 0 88 261 52 734.8 0 52 262 109 734.8 0 109 263 124 734.8 0 124 264 144 734.8 0 144 265 136 734.8 0 136 266 100 734.8 0 100 267 104 734.8 0 104 268 244 734.8 0 244 269 207 734.8 0 207 270 81 734.8 0 81 271 66 734.8 0 66 272 87 734.8 0 87 273 127 734.8 0 127 274 145 734.8 0 145 275 126 734.8 0 126 276 95 734.8 0 95 277 90 734.8 0 90 278 85 734.8 0 85 279 84 734.8 0 84 280 154 734.8 0 154 281 99 734.8 0 99 282 88 734.8 0 88 283 113 734.8 0 113 284 94 734.8 0 94 285 144 734.8 0 144 286 100 734.8 0 100 287 205 734.8 0 205 288 156 734.8 0 156 289 60 734.8 0 60 290 62 734.8 0 62 291 57 734.8 0 57 292 122 734.8 0 122 293 185 734.8 0 185 294 123 734.8 0 123 295 75 734.8 0 75 296 50 734.8 0 50 297 35 734.8 0 35 298 71 734.8 0 71 299 90 734.8 0 90 300 152 734.8 0 152 301 70 734.8 0 70 Finished in 0.99 s (41 us/read; 1.47 M reads/minute). === Summary === Total reads processed: 24,242 Reads with adapters: 1,937 (8.0%) Reads that were too short: 8 (0.0%) Reads written (passing filters): 1,929 (8.0%) Total basepairs processed: 5,859,812 bp Total written (filtered): 532,821 bp (9.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 1937 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 12 0.0 3 0 12 17 1531 0.0 3 1513 17 1 18 8 0.0 3 0 7 1 19 202 0.0 3 197 3 2 20 167 0.0 3 165 2 21 2 0.0 3 0 2 25 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 60 2 0.0 3 0 0 0 2 75 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.07 s (36 us/read; 1.65 M reads/minute). === Summary === Total reads processed: 1,929 Reads with adapters: 53 (2.7%) Reads written (passing filters): 1,929 (100.0%) Total basepairs processed: 532,821 bp Total written (filtered): 532,662 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 53 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 50.9% C: 17.0% G: 30.2% T: 1.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 53 30.1 0 53