This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACGCGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 68.55 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 378,563 (14.4%) Reads that were too short: 357,108 (13.6%) Reads written (passing filters): 21,455 (0.8%) Total basepairs processed: 791,719,999 bp Total written (filtered): 4,760,158 bp (0.6%) === Adapter 1 === Sequence: ACGCGC; Type: regular 5'; Length: 6; Trimmed: 378563 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 244 642.2 0 244 7 57 642.2 0 57 8 81 642.2 0 81 9 153 642.2 0 153 10 9299 642.2 0 9299 11 39 642.2 0 39 12 24 642.2 0 24 13 28 642.2 0 28 14 32 642.2 0 32 15 32 642.2 0 32 16 20 642.2 0 20 17 24 642.2 0 24 18 25 642.2 0 25 19 37 642.2 0 37 20 31 642.2 0 31 21 55 642.2 0 55 22 33 642.2 0 33 23 27 642.2 0 27 24 21 642.2 0 21 25 20 642.2 0 20 26 28 642.2 0 28 27 23 642.2 0 23 28 21 642.2 0 21 29 26 642.2 0 26 30 20 642.2 0 20 31 15 642.2 0 15 32 20 642.2 0 20 33 35 642.2 0 35 34 25 642.2 0 25 35 44 642.2 0 44 36 34 642.2 0 34 37 31 642.2 0 31 38 27 642.2 0 27 39 37 642.2 0 37 40 31 642.2 0 31 41 65 642.2 0 65 42 35 642.2 0 35 43 32 642.2 0 32 44 24 642.2 0 24 45 47 642.2 0 47 46 30 642.2 0 30 47 28 642.2 0 28 48 18 642.2 0 18 49 61 642.2 0 61 50 30 642.2 0 30 51 26 642.2 0 26 52 36 642.2 0 36 53 72 642.2 0 72 54 50 642.2 0 50 55 46 642.2 0 46 56 41 642.2 0 41 57 34 642.2 0 34 58 16 642.2 0 16 59 35 642.2 0 35 60 34 642.2 0 34 61 41 642.2 0 41 62 36 642.2 0 36 63 41 642.2 0 41 64 33 642.2 0 33 65 35 642.2 0 35 66 29 642.2 0 29 67 27 642.2 0 27 68 18 642.2 0 18 69 37 642.2 0 37 70 34 642.2 0 34 71 30 642.2 0 30 72 54 642.2 0 54 73 33 642.2 0 33 74 63 642.2 0 63 75 34 642.2 0 34 76 26 642.2 0 26 77 38 642.2 0 38 78 34 642.2 0 34 79 27 642.2 0 27 80 29 642.2 0 29 81 31 642.2 0 31 82 32 642.2 0 32 83 43 642.2 0 43 84 45 642.2 0 45 85 45 642.2 0 45 86 57 642.2 0 57 87 29 642.2 0 29 88 23 642.2 0 23 89 27 642.2 0 27 90 30 642.2 0 30 91 29 642.2 0 29 92 19 642.2 0 19 93 29 642.2 0 29 94 29 642.2 0 29 95 41 642.2 0 41 96 28 642.2 0 28 97 29 642.2 0 29 98 25 642.2 0 25 99 30 642.2 0 30 100 20 642.2 0 20 101 30 642.2 0 30 102 32 642.2 0 32 103 33 642.2 0 33 104 26 642.2 0 26 105 31 642.2 0 31 106 24 642.2 0 24 107 35 642.2 0 35 108 47 642.2 0 47 109 30 642.2 0 30 110 28 642.2 0 28 111 30 642.2 0 30 112 47 642.2 0 47 113 35 642.2 0 35 114 47 642.2 0 47 115 36 642.2 0 36 116 65 642.2 0 65 117 41 642.2 0 41 118 31 642.2 0 31 119 51 642.2 0 51 120 39 642.2 0 39 121 40 642.2 0 40 122 72 642.2 0 72 123 67 642.2 0 67 124 49 642.2 0 49 125 121 642.2 0 121 126 60 642.2 0 60 127 52 642.2 0 52 128 44 642.2 0 44 129 47 642.2 0 47 130 41 642.2 0 41 131 40 642.2 0 40 132 38 642.2 0 38 133 34 642.2 0 34 134 28 642.2 0 28 135 42 642.2 0 42 136 33 642.2 0 33 137 50 642.2 0 50 138 66 642.2 0 66 139 47 642.2 0 47 140 40 642.2 0 40 141 85 642.2 0 85 142 58 642.2 0 58 143 71 642.2 0 71 144 40 642.2 0 40 145 40 642.2 0 40 146 57 642.2 0 57 147 45 642.2 0 45 148 44 642.2 0 44 149 86 642.2 0 86 150 113 642.2 0 113 151 126 642.2 0 126 152 85 642.2 0 85 153 66 642.2 0 66 154 66 642.2 0 66 155 62 642.2 0 62 156 57 642.2 0 57 157 38 642.2 0 38 158 40 642.2 0 40 159 46 642.2 0 46 160 77 642.2 0 77 161 61 642.2 0 61 162 103 642.2 0 103 163 45 642.2 0 45 164 70 642.2 0 70 165 224 642.2 0 224 166 141 642.2 0 141 167 317 642.2 0 317 168 126 642.2 0 126 169 50 642.2 0 50 170 49 642.2 0 49 171 51 642.2 0 51 172 42 642.2 0 42 173 73 642.2 0 73 174 51 642.2 0 51 175 50 642.2 0 50 176 65 642.2 0 65 177 58 642.2 0 58 178 71 642.2 0 71 179 93 642.2 0 93 180 59 642.2 0 59 181 64 642.2 0 64 182 88 642.2 0 88 183 39 642.2 0 39 184 81 642.2 0 81 185 350 642.2 0 350 186 292 642.2 0 292 187 737 642.2 0 737 188 645 642.2 0 645 189 585 642.2 0 585 190 52 642.2 0 52 191 50 642.2 0 50 192 43 642.2 0 43 193 75 642.2 0 75 194 53 642.2 0 53 195 29 642.2 0 29 196 49 642.2 0 49 197 52 642.2 0 52 198 61 642.2 0 61 199 99 642.2 0 99 200 263 642.2 0 263 201 99 642.2 0 99 202 55 642.2 0 55 203 70 642.2 0 70 204 64 642.2 0 64 205 32 642.2 0 32 206 40 642.2 0 40 207 94 642.2 0 94 208 46 642.2 0 46 209 77 642.2 0 77 210 503 642.2 0 503 211 51 642.2 0 51 212 41 642.2 0 41 213 51 642.2 0 51 214 42 642.2 0 42 215 42 642.2 0 42 216 46 642.2 0 46 217 129 642.2 0 129 218 159 642.2 0 159 219 343 642.2 0 343 220 1104 642.2 0 1104 221 1231 642.2 0 1231 222 2901 642.2 0 2901 223 2128 642.2 0 2128 224 1063 642.2 0 1063 225 313 642.2 0 313 226 160 642.2 0 160 227 324 642.2 0 324 228 220 642.2 0 220 229 213 642.2 0 213 230 323 642.2 0 323 231 366 642.2 0 366 232 758 642.2 0 758 233 1033 642.2 0 1033 234 1673 642.2 0 1673 235 2225 642.2 0 2225 236 12173 642.2 0 12173 237 138774 642.2 0 138774 238 67155 642.2 0 67155 239 105193 642.2 0 105193 240 2970 642.2 0 2970 241 652 642.2 0 652 242 161 642.2 0 161 243 79 642.2 0 79 244 99 642.2 0 99 245 693 642.2 0 693 246 1396 642.2 0 1396 247 2140 642.2 0 2140 248 1531 642.2 0 1531 249 464 642.2 0 464 250 312 642.2 0 312 251 122 642.2 0 122 252 42 642.2 0 42 253 42 642.2 0 42 254 28 642.2 0 28 255 38 642.2 0 38 256 25 642.2 0 25 257 39 642.2 0 39 258 36 642.2 0 36 259 25 642.2 0 25 260 59 642.2 0 59 261 63 642.2 0 63 262 86 642.2 0 86 263 33 642.2 0 33 264 34 642.2 0 34 265 31 642.2 0 31 266 56 642.2 0 56 267 94 642.2 0 94 268 269 642.2 0 269 269 233 642.2 0 233 270 187 642.2 0 187 271 144 642.2 0 144 272 59 642.2 0 59 273 59 642.2 0 59 274 77 642.2 0 77 275 72 642.2 0 72 276 64 642.2 0 64 277 99 642.2 0 99 278 121 642.2 0 121 279 43 642.2 0 43 280 74 642.2 0 74 281 247 642.2 0 247 282 97 642.2 0 97 283 292 642.2 0 292 284 57 642.2 0 57 285 62 642.2 0 62 286 89 642.2 0 89 287 162 642.2 0 162 288 258 642.2 0 258 289 202 642.2 0 202 290 166 642.2 0 166 291 144 642.2 0 144 292 96 642.2 0 96 293 52 642.2 0 52 294 283 642.2 0 283 295 161 642.2 0 161 296 349 642.2 0 349 297 104 642.2 0 104 298 44 642.2 0 44 299 53 642.2 0 53 300 48 642.2 0 48 301 76 642.2 0 76 Finished in 0.99 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 21,455 Reads with adapters: 19 (0.1%) Reads that were too short: 11 (0.1%) Reads written (passing filters): 8 (0.0%) Total basepairs processed: 4,760,158 bp Total written (filtered): 1,941 bp (0.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 19 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 20 1 0.0 4 1 21 2 0.0 4 1 0 1 23 1 0.0 4 1 27 1 0.0 4 0 0 0 0 1 32 1 0.0 4 0 0 0 0 1 62 1 0.0 4 0 0 0 0 1 98 1 0.0 4 0 0 1 101 1 0.0 4 0 0 0 0 1 114 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 1 128 1 0.0 4 0 0 0 0 1 195 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (1250 us/read; 0.05 M reads/minute). === Summary === Total reads processed: 8 Reads with adapters: 0 (0.0%) Reads written (passing filters): 8 (100.0%) Total basepairs processed: 1,941 bp Total written (filtered): 1,941 bp (100.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 0 times.