This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCTAAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 68.11 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 282,667 (10.7%) Reads that were too short: 12,813 (0.5%) Reads written (passing filters): 269,854 (10.3%) Total basepairs processed: 791,719,999 bp Total written (filtered): 42,530,143 bp (5.4%) === Adapter 1 === Sequence: CCTAAT; Type: regular 5'; Length: 6; Trimmed: 282667 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 133 642.2 0 133 7 5535 642.2 0 5535 8 496 642.2 0 496 9 4001 642.2 0 4001 10 90 642.2 0 90 11 41 642.2 0 41 12 57 642.2 0 57 13 79 642.2 0 79 14 111 642.2 0 111 15 124 642.2 0 124 16 75 642.2 0 75 17 36 642.2 0 36 18 49 642.2 0 49 19 56 642.2 0 56 20 58 642.2 0 58 21 50 642.2 0 50 22 46 642.2 0 46 23 46 642.2 0 46 24 95 642.2 0 95 25 91 642.2 0 91 26 209 642.2 0 209 27 1085 642.2 0 1085 28 2404 642.2 0 2404 29 936 642.2 0 936 30 50 642.2 0 50 31 41 642.2 0 41 32 62 642.2 0 62 33 88 642.2 0 88 34 103 642.2 0 103 35 63 642.2 0 63 36 62 642.2 0 62 37 108 642.2 0 108 38 55 642.2 0 55 39 51 642.2 0 51 40 73 642.2 0 73 41 74 642.2 0 74 42 99 642.2 0 99 43 128 642.2 0 128 44 87 642.2 0 87 45 67 642.2 0 67 46 65 642.2 0 65 47 59 642.2 0 59 48 200 642.2 0 200 49 74 642.2 0 74 50 92 642.2 0 92 51 87 642.2 0 87 52 214 642.2 0 214 53 71 642.2 0 71 54 59 642.2 0 59 55 51 642.2 0 51 56 66 642.2 0 66 57 217 642.2 0 217 58 252 642.2 0 252 59 509 642.2 0 509 60 74 642.2 0 74 61 71 642.2 0 71 62 75 642.2 0 75 63 68 642.2 0 68 64 55 642.2 0 55 65 80 642.2 0 80 66 82 642.2 0 82 67 59 642.2 0 59 68 68 642.2 0 68 69 71 642.2 0 71 70 72 642.2 0 72 71 61 642.2 0 61 72 57 642.2 0 57 73 56 642.2 0 56 74 65 642.2 0 65 75 67 642.2 0 67 76 68 642.2 0 68 77 116 642.2 0 116 78 109 642.2 0 109 79 83 642.2 0 83 80 80 642.2 0 80 81 110 642.2 0 110 82 60 642.2 0 60 83 70 642.2 0 70 84 85 642.2 0 85 85 85 642.2 0 85 86 60 642.2 0 60 87 76 642.2 0 76 88 78 642.2 0 78 89 83 642.2 0 83 90 106 642.2 0 106 91 119 642.2 0 119 92 121 642.2 0 121 93 106 642.2 0 106 94 103 642.2 0 103 95 86 642.2 0 86 96 100 642.2 0 100 97 79 642.2 0 79 98 94 642.2 0 94 99 91 642.2 0 91 100 75 642.2 0 75 101 84 642.2 0 84 102 91 642.2 0 91 103 113 642.2 0 113 104 152 642.2 0 152 105 241 642.2 0 241 106 286 642.2 0 286 107 1243 642.2 0 1243 108 11053 642.2 0 11053 109 7501 642.2 0 7501 110 8149 642.2 0 8149 111 138 642.2 0 138 112 75 642.2 0 75 113 74 642.2 0 74 114 78 642.2 0 78 115 118 642.2 0 118 116 128 642.2 0 128 117 132 642.2 0 132 118 117 642.2 0 117 119 87 642.2 0 87 120 90 642.2 0 90 121 103 642.2 0 103 122 93 642.2 0 93 123 69 642.2 0 69 124 75 642.2 0 75 125 137 642.2 0 137 126 86 642.2 0 86 127 148 642.2 0 148 128 475 642.2 0 475 129 147 642.2 0 147 130 310 642.2 0 310 131 113 642.2 0 113 132 143 642.2 0 143 133 204 642.2 0 204 134 1238 642.2 0 1238 135 628 642.2 0 628 136 2403 642.2 0 2403 137 244 642.2 0 244 138 146 642.2 0 146 139 123 642.2 0 123 140 179 642.2 0 179 141 251 642.2 0 251 142 385 642.2 0 385 143 240 642.2 0 240 144 201 642.2 0 201 145 252 642.2 0 252 146 512 642.2 0 512 147 1070 642.2 0 1070 148 322 642.2 0 322 149 1231 642.2 0 1231 150 754 642.2 0 754 151 264 642.2 0 264 152 310 642.2 0 310 153 665 642.2 0 665 154 719 642.2 0 719 155 3478 642.2 0 3478 156 2741 642.2 0 2741 157 2972 642.2 0 2972 158 1160 642.2 0 1160 159 2465 642.2 0 2465 160 36239 642.2 0 36239 161 78014 642.2 0 78014 162 72103 642.2 0 72103 163 454 642.2 0 454 164 136 642.2 0 136 165 161 642.2 0 161 166 102 642.2 0 102 167 93 642.2 0 93 168 72 642.2 0 72 169 91 642.2 0 91 170 74 642.2 0 74 171 70 642.2 0 70 172 87 642.2 0 87 173 63 642.2 0 63 174 83 642.2 0 83 175 136 642.2 0 136 176 111 642.2 0 111 177 73 642.2 0 73 178 91 642.2 0 91 179 134 642.2 0 134 180 148 642.2 0 148 181 138 642.2 0 138 182 88 642.2 0 88 183 123 642.2 0 123 184 81 642.2 0 81 185 61 642.2 0 61 186 69 642.2 0 69 187 80 642.2 0 80 188 78 642.2 0 78 189 74 642.2 0 74 190 73 642.2 0 73 191 118 642.2 0 118 192 116 642.2 0 116 193 158 642.2 0 158 194 71 642.2 0 71 195 82 642.2 0 82 196 71 642.2 0 71 197 81 642.2 0 81 198 98 642.2 0 98 199 75 642.2 0 75 200 66 642.2 0 66 201 58 642.2 0 58 202 114 642.2 0 114 203 119 642.2 0 119 204 83 642.2 0 83 205 73 642.2 0 73 206 74 642.2 0 74 207 72 642.2 0 72 208 95 642.2 0 95 209 91 642.2 0 91 210 61 642.2 0 61 211 71 642.2 0 71 212 72 642.2 0 72 213 57 642.2 0 57 214 73 642.2 0 73 215 71 642.2 0 71 216 76 642.2 0 76 217 77 642.2 0 77 218 101 642.2 0 101 219 109 642.2 0 109 220 122 642.2 0 122 221 190 642.2 0 190 222 473 642.2 0 473 223 285 642.2 0 285 224 210 642.2 0 210 225 364 642.2 0 364 226 88 642.2 0 88 227 74 642.2 0 74 228 56 642.2 0 56 229 67 642.2 0 67 230 99 642.2 0 99 231 95 642.2 0 95 232 129 642.2 0 129 233 130 642.2 0 130 234 165 642.2 0 165 235 136 642.2 0 136 236 130 642.2 0 130 237 69 642.2 0 69 238 66 642.2 0 66 239 76 642.2 0 76 240 83 642.2 0 83 241 103 642.2 0 103 242 90 642.2 0 90 243 120 642.2 0 120 244 101 642.2 0 101 245 58 642.2 0 58 246 70 642.2 0 70 247 76 642.2 0 76 248 69 642.2 0 69 249 74 642.2 0 74 250 136 642.2 0 136 251 361 642.2 0 361 252 288 642.2 0 288 253 408 642.2 0 408 254 995 642.2 0 995 255 437 642.2 0 437 256 313 642.2 0 313 257 113 642.2 0 113 258 79 642.2 0 79 259 68 642.2 0 68 260 100 642.2 0 100 261 156 642.2 0 156 262 147 642.2 0 147 263 146 642.2 0 146 264 128 642.2 0 128 265 97 642.2 0 97 266 81 642.2 0 81 267 87 642.2 0 87 268 59 642.2 0 59 269 142 642.2 0 142 270 134 642.2 0 134 271 125 642.2 0 125 272 105 642.2 0 105 273 87 642.2 0 87 274 58 642.2 0 58 275 79 642.2 0 79 276 71 642.2 0 71 277 84 642.2 0 84 278 158 642.2 0 158 279 140 642.2 0 140 280 115 642.2 0 115 281 139 642.2 0 139 282 207 642.2 0 207 283 220 642.2 0 220 284 140 642.2 0 140 285 78 642.2 0 78 286 68 642.2 0 68 287 59 642.2 0 59 288 47 642.2 0 47 289 62 642.2 0 62 290 82 642.2 0 82 291 124 642.2 0 124 292 95 642.2 0 95 293 95 642.2 0 95 294 61 642.2 0 61 295 51 642.2 0 51 296 77 642.2 0 77 297 62 642.2 0 62 298 53 642.2 0 53 299 80 642.2 0 80 300 80 642.2 0 80 301 79 642.2 0 79 Finished in 8.50 s (31 us/read; 1.90 M reads/minute). === Summary === Total reads processed: 269,854 Reads with adapters: 10,287 (3.8%) Reads that were too short: 410 (0.2%) Reads written (passing filters): 9,877 (3.7%) Total basepairs processed: 42,530,143 bp Total written (filtered): 1,994,547 bp (4.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 10287 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 4 0.0 3 0 0 2 2 16 72 0.0 3 0 52 15 5 17 5321 0.0 3 4920 341 51 9 18 16 0.0 3 1 12 2 1 19 16 0.0 3 1 0 7 8 20 19 0.0 3 0 1 0 18 26 1 0.0 3 0 0 0 1 27 2 0.0 3 0 1 0 1 30 6 0.0 3 0 0 0 6 31 3972 0.0 3 0 0 35 3937 33 3 0.0 3 1 0 0 2 34 328 0.0 3 0 0 7 321 36 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 43 2 0.0 3 0 0 0 2 44 26 0.0 3 0 0 0 26 47 6 0.0 3 0 0 0 6 50 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 54 3 0.0 3 0 0 0 3 55 7 0.0 3 0 0 1 6 57 33 0.0 3 0 0 0 33 58 1 0.0 3 0 0 0 1 60 3 0.0 3 0 0 0 3 62 1 0.0 3 0 0 0 1 63 7 0.0 3 0 0 0 7 64 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 70 2 0.0 3 0 0 0 2 73 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 81 2 0.0 3 0 0 0 2 82 35 0.0 3 0 0 1 34 83 2 0.0 3 0 0 0 2 84 5 0.0 3 0 0 0 5 88 1 0.0 3 0 0 0 1 89 2 0.0 3 0 0 0 2 90 2 0.0 3 0 0 0 2 91 13 0.0 3 0 0 1 12 94 2 0.0 3 0 0 0 2 95 1 0.0 3 0 0 0 1 96 4 0.0 3 1 0 0 3 97 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 104 4 0.0 3 0 0 0 4 107 1 0.0 3 0 0 0 1 109 2 0.0 3 0 0 0 2 110 1 0.0 3 0 0 0 1 114 9 0.0 3 0 0 0 9 115 2 0.0 3 0 0 0 2 117 4 0.0 3 0 0 0 4 120 3 0.0 3 0 0 0 3 121 2 0.0 3 0 0 0 2 122 7 0.0 3 0 0 0 7 123 9 0.0 3 0 0 0 9 124 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 131 4 0.0 3 0 0 0 4 132 173 0.0 3 0 0 2 171 133 35 0.0 3 0 0 0 35 134 11 0.0 3 0 0 0 11 135 5 0.0 3 0 0 0 5 136 2 0.0 3 0 0 0 2 137 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 143 2 0.0 3 0 0 0 2 145 4 0.0 3 0 0 0 4 146 3 0.0 3 0 0 0 3 159 10 0.0 3 0 0 0 10 160 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 171 5 0.0 3 0 0 0 5 172 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 180 4 0.0 3 0 0 0 4 181 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 185 2 0.0 3 0 0 0 2 186 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 1 194 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 0 1 197 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 226 2 0.0 3 0 0 0 2 229 3 0.0 3 0 0 0 3 233 2 0.0 3 0 0 0 2 236 1 0.0 3 0 0 1 246 1 0.0 3 0 0 0 1 254 1 0.0 3 0 0 0 1 259 1 0.0 3 0 0 0 1 261 2 0.0 3 0 0 0 2 262 1 0.0 3 0 0 0 1 266 1 0.0 3 0 0 0 1 269 1 0.0 3 0 0 0 1 274 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 282 2 0.0 3 0 0 0 2 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.29 s (29 us/read; 2.04 M reads/minute). === Summary === Total reads processed: 9,877 Reads with adapters: 23 (0.2%) Reads written (passing filters): 9,877 (100.0%) Total basepairs processed: 1,994,547 bp Total written (filtered): 1,994,472 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 23 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 4.3% C: 65.2% G: 30.4% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 20 154.3 0 20 4 1 38.6 0 1 5 1 9.6 0 1 6 1 2.4 0 1