This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGGCCA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 68.45 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 150,437 (5.7%) Reads that were too short: 72,037 (2.7%) Reads written (passing filters): 78,400 (3.0%) Total basepairs processed: 791,719,999 bp Total written (filtered): 16,774,663 bp (2.1%) === Adapter 1 === Sequence: CGGCCA; Type: regular 5'; Length: 6; Trimmed: 150437 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2060 642.2 0 2060 7 13673 642.2 0 13673 8 906 642.2 0 906 9 14679 642.2 0 14679 10 102 642.2 0 102 11 1026 642.2 0 1026 12 49 642.2 0 49 13 57 642.2 0 57 14 40 642.2 0 40 15 37 642.2 0 37 16 26 642.2 0 26 17 38 642.2 0 38 18 41 642.2 0 41 19 27 642.2 0 27 20 36 642.2 0 36 21 60 642.2 0 60 22 42 642.2 0 42 23 37 642.2 0 37 24 37 642.2 0 37 25 34 642.2 0 34 26 34 642.2 0 34 27 35 642.2 0 35 28 46 642.2 0 46 29 28 642.2 0 28 30 49 642.2 0 49 31 61 642.2 0 61 32 52 642.2 0 52 33 52 642.2 0 52 34 42 642.2 0 42 35 52 642.2 0 52 36 43 642.2 0 43 37 39 642.2 0 39 38 59 642.2 0 59 39 52 642.2 0 52 40 109 642.2 0 109 41 260 642.2 0 260 42 257 642.2 0 257 43 188 642.2 0 188 44 36 642.2 0 36 45 24 642.2 0 24 46 53 642.2 0 53 47 47 642.2 0 47 48 51 642.2 0 51 49 55 642.2 0 55 50 119 642.2 0 119 51 66 642.2 0 66 52 66 642.2 0 66 53 56 642.2 0 56 54 70 642.2 0 70 55 57 642.2 0 57 56 60 642.2 0 60 57 48 642.2 0 48 58 31 642.2 0 31 59 30 642.2 0 30 60 44 642.2 0 44 61 36 642.2 0 36 62 41 642.2 0 41 63 53 642.2 0 53 64 249 642.2 0 249 65 314 642.2 0 314 66 257 642.2 0 257 67 61 642.2 0 61 68 44 642.2 0 44 69 39 642.2 0 39 70 42 642.2 0 42 71 64 642.2 0 64 72 58 642.2 0 58 73 256 642.2 0 256 74 1749 642.2 0 1749 75 865 642.2 0 865 76 495 642.2 0 495 77 55 642.2 0 55 78 78 642.2 0 78 79 50 642.2 0 50 80 47 642.2 0 47 81 40 642.2 0 40 82 59 642.2 0 59 83 75 642.2 0 75 84 42 642.2 0 42 85 30 642.2 0 30 86 57 642.2 0 57 87 312 642.2 0 312 88 178 642.2 0 178 89 368 642.2 0 368 90 46 642.2 0 46 91 35 642.2 0 35 92 58 642.2 0 58 93 55 642.2 0 55 94 48 642.2 0 48 95 44 642.2 0 44 96 37 642.2 0 37 97 53 642.2 0 53 98 62 642.2 0 62 99 58 642.2 0 58 100 45 642.2 0 45 101 75 642.2 0 75 102 34 642.2 0 34 103 51 642.2 0 51 104 64 642.2 0 64 105 60 642.2 0 60 106 44 642.2 0 44 107 37 642.2 0 37 108 40 642.2 0 40 109 58 642.2 0 58 110 40 642.2 0 40 111 53 642.2 0 53 112 44 642.2 0 44 113 44 642.2 0 44 114 147 642.2 0 147 115 55 642.2 0 55 116 61 642.2 0 61 117 70 642.2 0 70 118 54 642.2 0 54 119 39 642.2 0 39 120 45 642.2 0 45 121 51 642.2 0 51 122 40 642.2 0 40 123 63 642.2 0 63 124 49 642.2 0 49 125 115 642.2 0 115 126 137 642.2 0 137 127 73 642.2 0 73 128 55 642.2 0 55 129 56 642.2 0 56 130 54 642.2 0 54 131 60 642.2 0 60 132 87 642.2 0 87 133 74 642.2 0 74 134 61 642.2 0 61 135 56 642.2 0 56 136 57 642.2 0 57 137 107 642.2 0 107 138 144 642.2 0 144 139 83 642.2 0 83 140 41 642.2 0 41 141 57 642.2 0 57 142 75 642.2 0 75 143 167 642.2 0 167 144 89 642.2 0 89 145 87 642.2 0 87 146 94 642.2 0 94 147 97 642.2 0 97 148 95 642.2 0 95 149 83 642.2 0 83 150 137 642.2 0 137 151 833 642.2 0 833 152 412 642.2 0 412 153 1481 642.2 0 1481 154 303 642.2 0 303 155 853 642.2 0 853 156 4772 642.2 0 4772 157 1769 642.2 0 1769 158 5344 642.2 0 5344 159 1001 642.2 0 1001 160 185 642.2 0 185 161 265 642.2 0 265 162 129 642.2 0 129 163 297 642.2 0 297 164 174 642.2 0 174 165 219 642.2 0 219 166 270 642.2 0 270 167 144 642.2 0 144 168 105 642.2 0 105 169 158 642.2 0 158 170 261 642.2 0 261 171 1723 642.2 0 1723 172 447 642.2 0 447 173 311 642.2 0 311 174 129 642.2 0 129 175 234 642.2 0 234 176 1467 642.2 0 1467 177 2249 642.2 0 2249 178 2220 642.2 0 2220 179 984 642.2 0 984 180 333 642.2 0 333 181 184 642.2 0 184 182 336 642.2 0 336 183 223 642.2 0 223 184 154 642.2 0 154 185 97 642.2 0 97 186 79 642.2 0 79 187 136 642.2 0 136 188 627 642.2 0 627 189 297 642.2 0 297 190 137 642.2 0 137 191 205 642.2 0 205 192 95 642.2 0 95 193 99 642.2 0 99 194 91 642.2 0 91 195 126 642.2 0 126 196 111 642.2 0 111 197 84 642.2 0 84 198 46 642.2 0 46 199 83 642.2 0 83 200 246 642.2 0 246 201 380 642.2 0 380 202 267 642.2 0 267 203 104 642.2 0 104 204 54 642.2 0 54 205 111 642.2 0 111 206 111 642.2 0 111 207 104 642.2 0 104 208 105 642.2 0 105 209 60 642.2 0 60 210 75 642.2 0 75 211 70 642.2 0 70 212 65 642.2 0 65 213 90 642.2 0 90 214 183 642.2 0 183 215 501 642.2 0 501 216 240 642.2 0 240 217 86 642.2 0 86 218 111 642.2 0 111 219 85 642.2 0 85 220 99 642.2 0 99 221 103 642.2 0 103 222 83 642.2 0 83 223 136 642.2 0 136 224 113 642.2 0 113 225 153 642.2 0 153 226 132 642.2 0 132 227 105 642.2 0 105 228 297 642.2 0 297 229 290 642.2 0 290 230 242 642.2 0 242 231 131 642.2 0 131 232 164 642.2 0 164 233 651 642.2 0 651 234 335 642.2 0 335 235 246 642.2 0 246 236 102 642.2 0 102 237 367 642.2 0 367 238 294 642.2 0 294 239 167 642.2 0 167 240 202 642.2 0 202 241 113 642.2 0 113 242 122 642.2 0 122 243 109 642.2 0 109 244 101 642.2 0 101 245 102 642.2 0 102 246 88 642.2 0 88 247 99 642.2 0 99 248 271 642.2 0 271 249 631 642.2 0 631 250 620 642.2 0 620 251 477 642.2 0 477 252 375 642.2 0 375 253 1154 642.2 0 1154 254 531 642.2 0 531 255 949 642.2 0 949 256 150 642.2 0 150 257 181 642.2 0 181 258 322 642.2 0 322 259 980 642.2 0 980 260 825 642.2 0 825 261 3771 642.2 0 3771 262 11041 642.2 0 11041 263 13005 642.2 0 13005 264 6579 642.2 0 6579 265 2503 642.2 0 2503 266 101 642.2 0 101 267 132 642.2 0 132 268 138 642.2 0 138 269 211 642.2 0 211 270 92 642.2 0 92 271 85 642.2 0 85 272 113 642.2 0 113 273 140 642.2 0 140 274 179 642.2 0 179 275 287 642.2 0 287 276 186 642.2 0 186 277 282 642.2 0 282 278 259 642.2 0 259 279 194 642.2 0 194 280 176 642.2 0 176 281 178 642.2 0 178 282 401 642.2 0 401 283 286 642.2 0 286 284 329 642.2 0 329 285 168 642.2 0 168 286 231 642.2 0 231 287 1057 642.2 0 1057 288 2012 642.2 0 2012 289 1719 642.2 0 1719 290 333 642.2 0 333 291 392 642.2 0 392 292 974 642.2 0 974 293 853 642.2 0 853 294 604 642.2 0 604 295 604 642.2 0 604 296 409 642.2 0 409 297 609 642.2 0 609 298 1750 642.2 0 1750 299 1981 642.2 0 1981 300 2394 642.2 0 2394 301 245 642.2 0 245 Finished in 3.17 s (40 us/read; 1.48 M reads/minute). === Summary === Total reads processed: 78,400 Reads with adapters: 17,007 (21.7%) Reads that were too short: 352 (0.4%) Reads written (passing filters): 16,655 (21.2%) Total basepairs processed: 16,774,663 bp Total written (filtered): 4,443,899 bp (26.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 17007 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 5 0.0 2 0 0 0 5 15 629 0.0 3 0 0 16 613 16 101 0.0 3 0 82 9 10 17 15544 0.0 3 14313 992 191 48 18 135 0.0 3 3 32 8 92 19 49 0.0 3 0 0 26 23 20 15 0.0 3 1 0 0 14 21 2 0.0 3 0 0 0 2 24 4 0.0 3 0 0 0 4 28 2 0.0 3 0 0 1 1 29 11 0.0 3 0 0 0 11 30 1 0.0 3 0 0 0 1 31 2 0.0 3 0 0 0 2 32 2 0.0 3 0 0 0 2 35 2 0.0 3 0 0 0 2 36 3 0.0 3 0 0 0 3 37 3 0.0 3 0 0 0 3 38 2 0.0 3 0 0 0 2 39 14 0.0 3 0 0 1 13 40 1 0.0 3 0 0 0 1 41 27 0.0 3 0 0 0 27 42 6 0.0 3 0 0 0 6 43 1 0.0 3 0 0 0 1 44 3 0.0 3 0 0 0 3 46 7 0.0 3 0 0 0 7 49 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 51 2 0.0 3 0 0 0 2 53 2 0.0 3 0 0 0 2 54 2 0.0 3 0 0 0 2 56 1 0.0 3 0 0 0 1 57 2 0.0 3 0 0 0 2 58 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 60 2 0.0 3 0 0 0 2 61 1 0.0 3 0 0 0 1 65 2 0.0 3 0 0 0 2 66 30 0.0 3 0 0 0 30 67 6 0.0 3 0 0 1 5 68 6 0.0 3 0 0 0 6 69 2 0.0 3 0 0 0 2 70 2 0.0 3 0 0 0 2 71 1 0.0 3 0 0 0 1 75 9 0.0 3 0 0 0 9 76 2 0.0 3 0 0 0 2 77 2 0.0 3 0 0 0 2 78 3 0.0 3 0 0 0 3 80 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 83 2 0.0 3 0 0 0 2 84 13 0.0 3 0 0 1 12 85 4 0.0 3 0 0 0 4 87 6 0.0 3 0 0 0 6 88 4 0.0 3 0 0 0 4 90 1 0.0 3 0 0 0 1 91 2 0.0 3 0 0 0 2 93 2 0.0 3 0 0 1 1 94 3 0.0 3 0 0 0 3 95 3 0.0 3 0 0 1 2 99 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 101 2 0.0 3 0 0 1 1 102 1 0.0 3 0 0 0 1 103 4 0.0 3 0 0 0 4 104 2 0.0 3 0 0 0 2 105 2 0.0 3 0 0 0 2 106 4 0.0 3 0 0 0 4 107 3 0.0 3 0 0 0 3 108 3 0.0 3 0 0 0 3 110 1 0.0 3 0 0 0 1 113 3 0.0 3 0 0 1 2 115 4 0.0 3 0 0 0 4 116 8 0.0 3 0 0 0 8 117 15 0.0 3 0 0 0 15 118 11 0.0 3 0 0 1 10 120 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 127 39 0.0 3 0 0 1 38 128 8 0.0 3 0 0 0 8 129 6 0.0 3 0 0 1 5 130 19 0.0 3 0 0 5 14 131 6 0.0 3 0 0 1 5 135 5 0.0 3 0 0 0 5 136 3 0.0 3 0 0 0 3 137 2 0.0 3 0 0 1 1 140 3 0.0 3 0 0 0 3 141 3 0.0 3 0 0 1 2 142 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 145 20 0.0 3 0 0 1 19 146 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 150 6 0.0 3 0 0 0 6 151 14 0.0 3 0 0 0 14 153 1 0.0 3 0 0 0 1 154 2 0.0 3 0 0 0 2 155 3 0.0 3 0 0 0 3 157 3 0.0 3 0 0 0 3 158 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 162 12 0.0 3 0 0 0 12 163 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 165 3 0.0 3 0 0 0 3 169 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 1 182 9 0.0 3 0 0 0 9 183 4 0.0 3 0 0 0 4 184 1 0.0 3 0 0 0 1 185 3 0.0 3 0 0 0 3 187 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 194 2 0.0 3 0 0 1 1 195 1 0.0 3 0 0 0 1 197 2 0.0 3 0 0 0 2 198 1 0.0 3 0 0 1 199 1 0.0 3 0 0 0 1 200 1 0.0 3 0 0 0 1 202 3 0.0 3 0 0 1 2 205 3 0.0 3 0 0 0 3 213 1 0.0 3 0 0 1 214 1 0.0 3 0 0 0 1 215 1 0.0 3 0 0 0 1 222 2 0.0 3 0 0 0 2 227 1 0.0 3 0 0 0 1 233 1 0.0 3 0 0 0 1 234 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 241 1 0.0 3 0 0 0 1 243 1 0.0 3 0 0 0 1 245 2 0.0 3 0 0 0 2 253 4 0.0 3 0 0 1 3 261 1 0.0 3 0 0 0 1 264 1 0.0 3 0 0 0 1 265 2 0.0 3 0 0 0 2 268 1 0.0 3 0 0 0 1 274 1 0.0 3 0 0 0 1 277 2 0.0 3 0 0 0 2 278 1 0.0 3 0 0 1 279 2 0.0 3 0 0 0 2 281 6 0.0 3 0 0 0 6 282 4 0.0 3 0 0 0 4 283 1 0.0 3 0 0 0 1 284 1 0.0 3 0 0 0 1 285 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.58 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 16,655 Reads with adapters: 692 (4.2%) Reads written (passing filters): 16,655 (100.0%) Total basepairs processed: 4,443,899 bp Total written (filtered): 4,441,785 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 692 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 64.9% C: 17.2% G: 14.6% T: 3.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 655 260.2 0 655 4 36 65.1 0 36 5 1 16.3 0 1