This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TATCTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 68.46 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 376,176 (14.3%) Reads that were too short: 12,727 (0.5%) Reads written (passing filters): 363,449 (13.8%) Total basepairs processed: 791,719,999 bp Total written (filtered): 67,912,454 bp (8.6%) === Adapter 1 === Sequence: TATCTG; Type: regular 5'; Length: 6; Trimmed: 376176 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 208 642.2 0 208 7 167 642.2 0 167 8 2383 642.2 0 2383 9 2312 642.2 0 2312 10 104 642.2 0 104 11 103 642.2 0 103 12 92 642.2 0 92 13 87 642.2 0 87 14 115 642.2 0 115 15 114 642.2 0 114 16 116 642.2 0 116 17 130 642.2 0 130 18 123 642.2 0 123 19 109 642.2 0 109 20 90 642.2 0 90 21 99 642.2 0 99 22 120 642.2 0 120 23 104 642.2 0 104 24 138 642.2 0 138 25 276 642.2 0 276 26 390 642.2 0 390 27 322 642.2 0 322 28 89 642.2 0 89 29 102 642.2 0 102 30 99 642.2 0 99 31 131 642.2 0 131 32 144 642.2 0 144 33 117 642.2 0 117 34 94 642.2 0 94 35 108 642.2 0 108 36 118 642.2 0 118 37 209 642.2 0 209 38 143 642.2 0 143 39 125 642.2 0 125 40 119 642.2 0 119 41 85 642.2 0 85 42 94 642.2 0 94 43 135 642.2 0 135 44 105 642.2 0 105 45 96 642.2 0 96 46 91 642.2 0 91 47 125 642.2 0 125 48 86 642.2 0 86 49 87 642.2 0 87 50 65 642.2 0 65 51 95 642.2 0 95 52 82 642.2 0 82 53 97 642.2 0 97 54 78 642.2 0 78 55 114 642.2 0 114 56 94 642.2 0 94 57 94 642.2 0 94 58 91 642.2 0 91 59 84 642.2 0 84 60 115 642.2 0 115 61 75 642.2 0 75 62 94 642.2 0 94 63 109 642.2 0 109 64 107 642.2 0 107 65 88 642.2 0 88 66 124 642.2 0 124 67 90 642.2 0 90 68 101 642.2 0 101 69 178 642.2 0 178 70 596 642.2 0 596 71 688 642.2 0 688 72 1436 642.2 0 1436 73 95 642.2 0 95 74 116 642.2 0 116 75 88 642.2 0 88 76 106 642.2 0 106 77 122 642.2 0 122 78 135 642.2 0 135 79 91 642.2 0 91 80 99 642.2 0 99 81 98 642.2 0 98 82 121 642.2 0 121 83 161 642.2 0 161 84 335 642.2 0 335 85 110 642.2 0 110 86 327 642.2 0 327 87 97 642.2 0 97 88 88 642.2 0 88 89 84 642.2 0 84 90 90 642.2 0 90 91 92 642.2 0 92 92 99 642.2 0 99 93 132 642.2 0 132 94 96 642.2 0 96 95 222 642.2 0 222 96 417 642.2 0 417 97 150 642.2 0 150 98 232 642.2 0 232 99 190 642.2 0 190 100 175 642.2 0 175 101 207 642.2 0 207 102 151 642.2 0 151 103 179 642.2 0 179 104 139 642.2 0 139 105 187 642.2 0 187 106 324 642.2 0 324 107 405 642.2 0 405 108 763 642.2 0 763 109 1098 642.2 0 1098 110 1486 642.2 0 1486 111 2278 642.2 0 2278 112 14231 642.2 0 14231 113 133444 642.2 0 133444 114 82216 642.2 0 82216 115 48141 642.2 0 48141 116 325 642.2 0 325 117 119 642.2 0 119 118 154 642.2 0 154 119 120 642.2 0 120 120 100 642.2 0 100 121 134 642.2 0 134 122 179 642.2 0 179 123 126 642.2 0 126 124 152 642.2 0 152 125 212 642.2 0 212 126 156 642.2 0 156 127 382 642.2 0 382 128 295 642.2 0 295 129 247 642.2 0 247 130 295 642.2 0 295 131 252 642.2 0 252 132 332 642.2 0 332 133 526 642.2 0 526 134 1341 642.2 0 1341 135 32878 642.2 0 32878 136 4902 642.2 0 4902 137 5699 642.2 0 5699 138 343 642.2 0 343 139 267 642.2 0 267 140 267 642.2 0 267 141 742 642.2 0 742 142 140 642.2 0 140 143 236 642.2 0 236 144 166 642.2 0 166 145 974 642.2 0 974 146 115 642.2 0 115 147 115 642.2 0 115 148 229 642.2 0 229 149 117 642.2 0 117 150 190 642.2 0 190 151 90 642.2 0 90 152 104 642.2 0 104 153 86 642.2 0 86 154 95 642.2 0 95 155 99 642.2 0 99 156 114 642.2 0 114 157 122 642.2 0 122 158 201 642.2 0 201 159 400 642.2 0 400 160 214 642.2 0 214 161 151 642.2 0 151 162 81 642.2 0 81 163 102 642.2 0 102 164 254 642.2 0 254 165 105 642.2 0 105 166 151 642.2 0 151 167 96 642.2 0 96 168 118 642.2 0 118 169 87 642.2 0 87 170 126 642.2 0 126 171 92 642.2 0 92 172 63 642.2 0 63 173 88 642.2 0 88 174 86 642.2 0 86 175 104 642.2 0 104 176 120 642.2 0 120 177 99 642.2 0 99 178 96 642.2 0 96 179 126 642.2 0 126 180 105 642.2 0 105 181 135 642.2 0 135 182 145 642.2 0 145 183 141 642.2 0 141 184 107 642.2 0 107 185 148 642.2 0 148 186 108 642.2 0 108 187 96 642.2 0 96 188 175 642.2 0 175 189 96 642.2 0 96 190 109 642.2 0 109 191 93 642.2 0 93 192 126 642.2 0 126 193 99 642.2 0 99 194 82 642.2 0 82 195 107 642.2 0 107 196 121 642.2 0 121 197 96 642.2 0 96 198 103 642.2 0 103 199 151 642.2 0 151 200 121 642.2 0 121 201 172 642.2 0 172 202 173 642.2 0 173 203 219 642.2 0 219 204 171 642.2 0 171 205 133 642.2 0 133 206 238 642.2 0 238 207 141 642.2 0 141 208 158 642.2 0 158 209 94 642.2 0 94 210 96 642.2 0 96 211 101 642.2 0 101 212 108 642.2 0 108 213 105 642.2 0 105 214 131 642.2 0 131 215 125 642.2 0 125 216 145 642.2 0 145 217 98 642.2 0 98 218 83 642.2 0 83 219 130 642.2 0 130 220 117 642.2 0 117 221 152 642.2 0 152 222 88 642.2 0 88 223 126 642.2 0 126 224 334 642.2 0 334 225 375 642.2 0 375 226 364 642.2 0 364 227 80 642.2 0 80 228 79 642.2 0 79 229 88 642.2 0 88 230 80 642.2 0 80 231 72 642.2 0 72 232 83 642.2 0 83 233 103 642.2 0 103 234 200 642.2 0 200 235 236 642.2 0 236 236 223 642.2 0 223 237 85 642.2 0 85 238 77 642.2 0 77 239 75 642.2 0 75 240 81 642.2 0 81 241 95 642.2 0 95 242 100 642.2 0 100 243 84 642.2 0 84 244 113 642.2 0 113 245 118 642.2 0 118 246 120 642.2 0 120 247 78 642.2 0 78 248 94 642.2 0 94 249 102 642.2 0 102 250 103 642.2 0 103 251 979 642.2 0 979 252 89 642.2 0 89 253 75 642.2 0 75 254 70 642.2 0 70 255 111 642.2 0 111 256 101 642.2 0 101 257 115 642.2 0 115 258 81 642.2 0 81 259 103 642.2 0 103 260 80 642.2 0 80 261 89 642.2 0 89 262 68 642.2 0 68 263 71 642.2 0 71 264 93 642.2 0 93 265 76 642.2 0 76 266 67 642.2 0 67 267 79 642.2 0 79 268 110 642.2 0 110 269 112 642.2 0 112 270 98 642.2 0 98 271 110 642.2 0 110 272 173 642.2 0 173 273 161 642.2 0 161 274 211 642.2 0 211 275 149 642.2 0 149 276 100 642.2 0 100 277 83 642.2 0 83 278 62 642.2 0 62 279 81 642.2 0 81 280 132 642.2 0 132 281 111 642.2 0 111 282 170 642.2 0 170 283 99 642.2 0 99 284 85 642.2 0 85 285 85 642.2 0 85 286 81 642.2 0 81 287 109 642.2 0 109 288 93 642.2 0 93 289 93 642.2 0 93 290 77 642.2 0 77 291 103 642.2 0 103 292 88 642.2 0 88 293 176 642.2 0 176 294 154 642.2 0 154 295 195 642.2 0 195 296 115 642.2 0 115 297 108 642.2 0 108 298 76 642.2 0 76 299 68 642.2 0 68 300 66 642.2 0 66 301 72 642.2 0 72 Finished in 11.99 s (33 us/read; 1.82 M reads/minute). === Summary === Total reads processed: 363,449 Reads with adapters: 3,102 (0.9%) Reads that were too short: 550 (0.2%) Reads written (passing filters): 2,552 (0.7%) Total basepairs processed: 67,912,454 bp Total written (filtered): 675,529 bp (1.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 3102 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 3 0.0 3 0 0 1 2 16 26 0.0 3 0 23 2 1 17 2297 0.0 3 2200 80 13 4 18 23 0.0 3 13 8 2 19 1 0.0 3 0 0 1 20 4 0.0 3 0 2 1 1 21 5 0.0 3 0 0 1 4 30 1 0.0 3 0 0 0 1 33 48 0.0 3 0 0 1 47 35 2 0.0 3 0 0 0 2 36 13 0.0 3 0 0 0 13 38 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 43 5 0.0 3 0 0 0 5 44 1 0.0 3 1 45 3 0.0 3 0 0 0 3 46 7 0.0 3 0 0 1 6 47 1 0.0 3 0 1 48 2 0.0 3 0 0 0 2 49 3 0.0 3 0 1 0 2 50 4 0.0 3 0 0 0 4 51 3 0.0 3 0 0 0 3 52 8 0.0 3 0 0 0 8 53 1 0.0 3 0 0 0 1 54 21 0.0 3 0 0 0 21 55 1 0.0 3 0 0 0 1 57 7 0.0 3 0 0 0 7 58 8 0.0 3 0 0 0 8 59 3 0.0 3 0 0 1 2 60 1 0.0 3 0 0 0 1 62 2 0.0 3 0 0 0 2 63 2 0.0 3 0 0 0 2 64 2 0.0 3 0 0 0 2 66 1 0.0 3 0 0 0 1 67 2 0.0 3 0 0 0 2 72 4 0.0 3 0 0 0 4 73 5 0.0 3 0 0 0 5 74 1 0.0 3 1 75 1 0.0 3 1 76 3 0.0 3 0 0 0 3 77 3 0.0 3 1 0 0 2 78 4 0.0 3 0 0 0 4 79 2 0.0 3 0 0 0 2 80 1 0.0 3 0 0 0 1 84 4 0.0 3 0 0 1 3 85 1 0.0 3 0 0 0 1 86 4 0.0 3 0 0 0 4 87 2 0.0 3 0 0 0 2 89 4 0.0 3 0 0 0 4 93 2 0.0 3 0 0 0 2 97 1 0.0 3 0 0 0 1 98 2 0.0 3 0 0 0 2 99 83 0.0 3 0 0 0 83 100 5 0.0 3 0 0 0 5 102 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 111 124 0.0 3 0 0 16 108 112 10 0.0 3 0 0 0 10 115 26 0.0 3 0 0 1 25 116 9 0.0 3 0 0 0 9 117 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 130 2 0.0 3 0 0 0 2 131 1 0.0 3 0 0 0 1 132 5 0.0 3 0 0 0 5 133 3 0.0 3 0 0 0 3 134 5 0.0 3 0 0 0 5 135 7 0.0 3 0 0 0 7 136 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 143 2 0.0 3 0 0 0 2 145 2 0.0 3 0 0 0 2 146 2 0.0 3 0 0 0 2 148 2 0.0 3 0 0 0 2 150 1 0.0 3 0 0 0 1 154 6 0.0 3 0 0 1 5 155 2 0.0 3 0 1 0 1 156 1 0.0 3 0 0 0 1 157 3 0.0 3 0 0 0 3 158 2 0.0 3 0 0 0 2 159 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 163 2 0.0 3 0 0 0 2 169 2 0.0 3 0 0 0 2 171 1 0.0 3 0 0 0 1 172 2 0.0 3 0 0 0 2 173 30 0.0 3 0 0 0 30 174 26 0.0 3 0 0 0 26 175 62 0.0 3 0 0 3 59 176 56 0.0 3 0 0 2 54 177 15 0.0 3 0 0 1 14 179 3 0.0 3 0 0 0 3 180 2 0.0 3 0 0 0 2 181 1 0.0 3 0 0 0 1 182 2 0.0 3 0 0 0 2 183 4 0.0 3 0 0 0 4 184 3 0.0 3 0 0 0 3 185 1 0.0 3 0 0 0 1 186 4 0.0 3 0 0 0 4 187 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 219 2 0.0 3 0 0 0 2 226 1 0.0 3 0 0 0 1 241 1 0.0 3 0 0 0 1 261 1 0.0 3 0 0 0 1 265 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 289 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.08 s (31 us/read; 1.91 M reads/minute). === Summary === Total reads processed: 2,552 Reads with adapters: 52 (2.0%) Reads written (passing filters): 2,552 (100.0%) Total basepairs processed: 675,529 bp Total written (filtered): 675,363 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 52 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 28.8% C: 23.1% G: 34.6% T: 13.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 43 39.9 0 43 4 8 10.0 0 8 5 1 2.5 0 1