This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TATCTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 70.59 s (27 us/read; 2.24 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 407,683 (15.5%) Reads that were too short: 8,814 (0.3%) Reads written (passing filters): 398,869 (15.2%) Total basepairs processed: 791,719,999 bp Total written (filtered): 74,578,678 bp (9.4%) === Adapter 1 === Sequence: TATCTG; Type: regular 5'; Length: 6; Trimmed: 407683 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 138 642.2 0 138 7 153 642.2 0 153 8 2410 642.2 0 2410 9 2404 642.2 0 2404 10 80 642.2 0 80 11 84 642.2 0 84 12 89 642.2 0 89 13 85 642.2 0 85 14 110 642.2 0 110 15 106 642.2 0 106 16 82 642.2 0 82 17 125 642.2 0 125 18 122 642.2 0 122 19 96 642.2 0 96 20 79 642.2 0 79 21 96 642.2 0 96 22 94 642.2 0 94 23 99 642.2 0 99 24 116 642.2 0 116 25 165 642.2 0 165 26 176 642.2 0 176 27 128 642.2 0 128 28 98 642.2 0 98 29 88 642.2 0 88 30 120 642.2 0 120 31 108 642.2 0 108 32 138 642.2 0 138 33 109 642.2 0 109 34 100 642.2 0 100 35 79 642.2 0 79 36 89 642.2 0 89 37 120 642.2 0 120 38 114 642.2 0 114 39 86 642.2 0 86 40 90 642.2 0 90 41 84 642.2 0 84 42 87 642.2 0 87 43 168 642.2 0 168 44 74 642.2 0 74 45 74 642.2 0 74 46 81 642.2 0 81 47 71 642.2 0 71 48 64 642.2 0 64 49 81 642.2 0 81 50 51 642.2 0 51 51 66 642.2 0 66 52 76 642.2 0 76 53 90 642.2 0 90 54 66 642.2 0 66 55 127 642.2 0 127 56 108 642.2 0 108 57 95 642.2 0 95 58 86 642.2 0 86 59 76 642.2 0 76 60 77 642.2 0 77 61 73 642.2 0 73 62 65 642.2 0 65 63 107 642.2 0 107 64 78 642.2 0 78 65 73 642.2 0 73 66 80 642.2 0 80 67 81 642.2 0 81 68 105 642.2 0 105 69 171 642.2 0 171 70 659 642.2 0 659 71 746 642.2 0 746 72 1502 642.2 0 1502 73 99 642.2 0 99 74 95 642.2 0 95 75 80 642.2 0 80 76 138 642.2 0 138 77 113 642.2 0 113 78 109 642.2 0 109 79 90 642.2 0 90 80 75 642.2 0 75 81 90 642.2 0 90 82 95 642.2 0 95 83 143 642.2 0 143 84 300 642.2 0 300 85 134 642.2 0 134 86 433 642.2 0 433 87 89 642.2 0 89 88 92 642.2 0 92 89 95 642.2 0 95 90 83 642.2 0 83 91 71 642.2 0 71 92 112 642.2 0 112 93 142 642.2 0 142 94 113 642.2 0 113 95 203 642.2 0 203 96 528 642.2 0 528 97 152 642.2 0 152 98 224 642.2 0 224 99 186 642.2 0 186 100 187 642.2 0 187 101 296 642.2 0 296 102 153 642.2 0 153 103 162 642.2 0 162 104 151 642.2 0 151 105 194 642.2 0 194 106 277 642.2 0 277 107 447 642.2 0 447 108 770 642.2 0 770 109 1095 642.2 0 1095 110 1609 642.2 0 1609 111 2239 642.2 0 2239 112 12079 642.2 0 12079 113 146919 642.2 0 146919 114 98136 642.2 0 98136 115 59659 642.2 0 59659 116 331 642.2 0 331 117 113 642.2 0 113 118 156 642.2 0 156 119 110 642.2 0 110 120 90 642.2 0 90 121 110 642.2 0 110 122 166 642.2 0 166 123 121 642.2 0 121 124 130 642.2 0 130 125 216 642.2 0 216 126 113 642.2 0 113 127 379 642.2 0 379 128 263 642.2 0 263 129 227 642.2 0 227 130 211 642.2 0 211 131 218 642.2 0 218 132 319 642.2 0 319 133 471 642.2 0 471 134 1030 642.2 0 1030 135 33063 642.2 0 33063 136 5081 642.2 0 5081 137 5918 642.2 0 5918 138 240 642.2 0 240 139 201 642.2 0 201 140 179 642.2 0 179 141 815 642.2 0 815 142 128 642.2 0 128 143 223 642.2 0 223 144 145 642.2 0 145 145 1003 642.2 0 1003 146 94 642.2 0 94 147 85 642.2 0 85 148 156 642.2 0 156 149 83 642.2 0 83 150 126 642.2 0 126 151 69 642.2 0 69 152 74 642.2 0 74 153 81 642.2 0 81 154 83 642.2 0 83 155 66 642.2 0 66 156 87 642.2 0 87 157 75 642.2 0 75 158 117 642.2 0 117 159 183 642.2 0 183 160 113 642.2 0 113 161 88 642.2 0 88 162 86 642.2 0 86 163 87 642.2 0 87 164 100 642.2 0 100 165 83 642.2 0 83 166 78 642.2 0 78 167 82 642.2 0 82 168 75 642.2 0 75 169 79 642.2 0 79 170 100 642.2 0 100 171 87 642.2 0 87 172 50 642.2 0 50 173 68 642.2 0 68 174 59 642.2 0 59 175 83 642.2 0 83 176 77 642.2 0 77 177 73 642.2 0 73 178 59 642.2 0 59 179 74 642.2 0 74 180 82 642.2 0 82 181 138 642.2 0 138 182 125 642.2 0 125 183 88 642.2 0 88 184 61 642.2 0 61 185 85 642.2 0 85 186 72 642.2 0 72 187 74 642.2 0 74 188 110 642.2 0 110 189 70 642.2 0 70 190 102 642.2 0 102 191 73 642.2 0 73 192 95 642.2 0 95 193 84 642.2 0 84 194 65 642.2 0 65 195 47 642.2 0 47 196 83 642.2 0 83 197 62 642.2 0 62 198 84 642.2 0 84 199 70 642.2 0 70 200 74 642.2 0 74 201 100 642.2 0 100 202 90 642.2 0 90 203 162 642.2 0 162 204 91 642.2 0 91 205 77 642.2 0 77 206 102 642.2 0 102 207 79 642.2 0 79 208 101 642.2 0 101 209 64 642.2 0 64 210 62 642.2 0 62 211 72 642.2 0 72 212 51 642.2 0 51 213 64 642.2 0 64 214 44 642.2 0 44 215 56 642.2 0 56 216 61 642.2 0 61 217 60 642.2 0 60 218 47 642.2 0 47 219 55 642.2 0 55 220 77 642.2 0 77 221 101 642.2 0 101 222 71 642.2 0 71 223 71 642.2 0 71 224 317 642.2 0 317 225 214 642.2 0 214 226 172 642.2 0 172 227 62 642.2 0 62 228 65 642.2 0 65 229 73 642.2 0 73 230 59 642.2 0 59 231 52 642.2 0 52 232 56 642.2 0 56 233 52 642.2 0 52 234 83 642.2 0 83 235 102 642.2 0 102 236 84 642.2 0 84 237 78 642.2 0 78 238 45 642.2 0 45 239 90 642.2 0 90 240 58 642.2 0 58 241 93 642.2 0 93 242 80 642.2 0 80 243 75 642.2 0 75 244 101 642.2 0 101 245 82 642.2 0 82 246 70 642.2 0 70 247 70 642.2 0 70 248 70 642.2 0 70 249 90 642.2 0 90 250 92 642.2 0 92 251 1067 642.2 0 1067 252 75 642.2 0 75 253 58 642.2 0 58 254 61 642.2 0 61 255 79 642.2 0 79 256 70 642.2 0 70 257 83 642.2 0 83 258 75 642.2 0 75 259 74 642.2 0 74 260 71 642.2 0 71 261 66 642.2 0 66 262 59 642.2 0 59 263 48 642.2 0 48 264 65 642.2 0 65 265 57 642.2 0 57 266 54 642.2 0 54 267 68 642.2 0 68 268 67 642.2 0 67 269 52 642.2 0 52 270 59 642.2 0 59 271 107 642.2 0 107 272 152 642.2 0 152 273 115 642.2 0 115 274 125 642.2 0 125 275 85 642.2 0 85 276 68 642.2 0 68 277 52 642.2 0 52 278 73 642.2 0 73 279 67 642.2 0 67 280 105 642.2 0 105 281 88 642.2 0 88 282 123 642.2 0 123 283 75 642.2 0 75 284 72 642.2 0 72 285 66 642.2 0 66 286 60 642.2 0 60 287 60 642.2 0 60 288 52 642.2 0 52 289 58 642.2 0 58 290 57 642.2 0 57 291 76 642.2 0 76 292 66 642.2 0 66 293 99 642.2 0 99 294 79 642.2 0 79 295 83 642.2 0 83 296 57 642.2 0 57 297 69 642.2 0 69 298 56 642.2 0 56 299 48 642.2 0 48 300 38 642.2 0 38 301 32 642.2 0 32 Finished in 14.78 s (37 us/read; 1.62 M reads/minute). === Summary === Total reads processed: 398,869 Reads with adapters: 2,427 (0.6%) Reads that were too short: 12 (0.0%) Reads written (passing filters): 2,415 (0.6%) Total basepairs processed: 74,578,678 bp Total written (filtered): 654,574 bp (0.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 2427 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 19 4 0.0 3 0 4 20 2369 0.0 4 2333 33 3 21 9 0.0 4 3 6 22 2 0.0 4 0 0 2 24 1 0.0 4 0 0 0 0 1 26 1 0.0 4 1 32 1 0.0 4 1 40 1 0.0 4 1 43 1 0.0 4 1 51 2 0.0 4 2 60 1 0.0 4 1 62 1 0.0 4 1 72 1 0.0 4 0 1 73 1 0.0 4 1 77 2 0.0 4 2 78 4 0.0 4 3 1 79 4 0.0 4 4 80 5 0.0 4 5 81 2 0.0 4 2 83 1 0.0 4 0 0 0 0 1 89 1 0.0 4 1 122 2 0.0 4 0 0 0 0 2 131 2 0.0 4 0 0 0 0 2 134 1 0.0 4 1 136 1 0.0 4 1 137 1 0.0 4 0 1 140 1 0.0 4 0 1 141 1 0.0 4 0 0 0 0 1 150 1 0.0 4 0 0 0 0 1 169 1 0.0 4 0 0 0 0 1 279 1 0.0 4 0 0 0 0 1 291 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.08 s (33 us/read; 1.81 M reads/minute). === Summary === Total reads processed: 2,415 Reads with adapters: 2,013 (83.4%) Reads written (passing filters): 2,415 (100.0%) Total basepairs processed: 654,574 bp Total written (filtered): 127,079 bp (19.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2013 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.2% C: 0.3% G: 1.4% T: 98.1% none/other: 0.0% WARNING: The adapter is preceded by "T" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "T" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 4 1 9.4 0 1 9 2 0.0 0 1 1 10 30 0.0 1 1 29 11 2 0.0 1 0 2 251 1 0.0 1 1 263 1 0.0 1 1 265 2 0.0 1 2 266 1014 0.0 1 939 75 267 919 0.0 1 872 47 268 23 0.0 1 22 1 269 14 0.0 1 12 2 270 4 0.0 1 4 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.