This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGATC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 68.43 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 35,094 (1.3%) Reads that were too short: 8,896 (0.3%) Reads written (passing filters): 26,198 (1.0%) Total basepairs processed: 791,719,999 bp Total written (filtered): 6,030,384 bp (0.8%) === Adapter 1 === Sequence: GTGATC; Type: regular 5'; Length: 6; Trimmed: 35094 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3525 642.2 0 3525 7 3627 642.2 0 3627 8 2648 642.2 0 2648 9 2173 642.2 0 2173 10 23 642.2 0 23 11 2 642.2 0 2 12 3 642.2 0 3 13 5 642.2 0 5 14 4 642.2 0 4 15 6 642.2 0 6 16 2 642.2 0 2 17 4 642.2 0 4 18 4 642.2 0 4 19 8 642.2 0 8 20 6 642.2 0 6 21 10 642.2 0 10 22 16 642.2 0 16 23 26 642.2 0 26 24 19 642.2 0 19 25 10 642.2 0 10 26 1 642.2 0 1 27 5 642.2 0 5 28 6 642.2 0 6 29 17 642.2 0 17 30 23 642.2 0 23 31 18 642.2 0 18 32 7 642.2 0 7 33 172 642.2 0 172 34 36 642.2 0 36 35 27 642.2 0 27 36 80 642.2 0 80 37 7 642.2 0 7 38 44 642.2 0 44 39 47 642.2 0 47 40 49 642.2 0 49 41 14 642.2 0 14 42 6 642.2 0 6 43 6 642.2 0 6 44 18 642.2 0 18 45 15 642.2 0 15 46 19 642.2 0 19 47 3 642.2 0 3 48 8 642.2 0 8 49 5 642.2 0 5 50 13 642.2 0 13 51 24 642.2 0 24 52 12 642.2 0 12 53 11 642.2 0 11 54 9 642.2 0 9 55 20 642.2 0 20 56 20 642.2 0 20 57 13 642.2 0 13 58 77 642.2 0 77 59 59 642.2 0 59 60 151 642.2 0 151 61 6 642.2 0 6 62 7 642.2 0 7 63 15 642.2 0 15 64 8 642.2 0 8 65 6 642.2 0 6 66 7 642.2 0 7 67 17 642.2 0 17 68 4 642.2 0 4 69 17 642.2 0 17 70 11 642.2 0 11 71 48 642.2 0 48 72 52 642.2 0 52 73 17 642.2 0 17 74 11 642.2 0 11 75 6 642.2 0 6 76 106 642.2 0 106 77 61 642.2 0 61 78 176 642.2 0 176 79 22 642.2 0 22 80 202 642.2 0 202 81 366 642.2 0 366 82 186 642.2 0 186 83 11 642.2 0 11 84 7 642.2 0 7 85 43 642.2 0 43 86 25 642.2 0 25 87 29 642.2 0 29 88 21 642.2 0 21 89 17 642.2 0 17 90 9 642.2 0 9 91 14 642.2 0 14 92 4 642.2 0 4 93 13 642.2 0 13 94 19 642.2 0 19 95 12 642.2 0 12 96 16 642.2 0 16 97 19 642.2 0 19 98 23 642.2 0 23 99 108 642.2 0 108 100 1265 642.2 0 1265 101 1484 642.2 0 1484 102 1281 642.2 0 1281 103 12 642.2 0 12 104 8 642.2 0 8 105 10 642.2 0 10 106 12 642.2 0 12 107 6 642.2 0 6 108 13 642.2 0 13 109 58 642.2 0 58 110 466 642.2 0 466 111 89 642.2 0 89 112 39 642.2 0 39 113 22 642.2 0 22 114 9 642.2 0 9 115 16 642.2 0 16 116 137 642.2 0 137 117 15 642.2 0 15 118 8 642.2 0 8 119 18 642.2 0 18 120 27 642.2 0 27 121 15 642.2 0 15 122 14 642.2 0 14 123 36 642.2 0 36 124 722 642.2 0 722 125 13 642.2 0 13 126 11 642.2 0 11 127 13 642.2 0 13 128 60 642.2 0 60 129 11 642.2 0 11 130 19 642.2 0 19 131 30 642.2 0 30 132 22 642.2 0 22 133 34 642.2 0 34 134 12 642.2 0 12 135 15 642.2 0 15 136 14 642.2 0 14 137 11 642.2 0 11 138 15 642.2 0 15 139 14 642.2 0 14 140 26 642.2 0 26 141 19 642.2 0 19 142 23 642.2 0 23 143 13 642.2 0 13 144 21 642.2 0 21 145 16 642.2 0 16 146 27 642.2 0 27 147 44 642.2 0 44 148 56 642.2 0 56 149 109 642.2 0 109 150 53 642.2 0 53 151 1097 642.2 0 1097 152 41 642.2 0 41 153 39 642.2 0 39 154 24 642.2 0 24 155 9 642.2 0 9 156 17 642.2 0 17 157 103 642.2 0 103 158 46 642.2 0 46 159 54 642.2 0 54 160 27 642.2 0 27 161 26 642.2 0 26 162 18 642.2 0 18 163 15 642.2 0 15 164 14 642.2 0 14 165 9 642.2 0 9 166 15 642.2 0 15 167 15 642.2 0 15 168 60 642.2 0 60 169 39 642.2 0 39 170 125 642.2 0 125 171 9 642.2 0 9 172 12 642.2 0 12 173 7 642.2 0 7 174 12 642.2 0 12 175 11 642.2 0 11 176 9 642.2 0 9 177 35 642.2 0 35 178 24 642.2 0 24 179 39 642.2 0 39 180 23 642.2 0 23 181 19 642.2 0 19 182 78 642.2 0 78 183 85 642.2 0 85 184 121 642.2 0 121 185 73 642.2 0 73 186 31 642.2 0 31 187 17 642.2 0 17 188 24 642.2 0 24 189 30 642.2 0 30 190 25 642.2 0 25 191 33 642.2 0 33 192 15 642.2 0 15 193 27 642.2 0 27 194 41 642.2 0 41 195 24 642.2 0 24 196 35 642.2 0 35 197 128 642.2 0 128 198 266 642.2 0 266 199 1317 642.2 0 1317 200 202 642.2 0 202 201 246 642.2 0 246 202 345 642.2 0 345 203 180 642.2 0 180 204 467 642.2 0 467 205 106 642.2 0 106 206 72 642.2 0 72 207 32 642.2 0 32 208 25 642.2 0 25 209 66 642.2 0 66 210 41 642.2 0 41 211 29 642.2 0 29 212 27 642.2 0 27 213 38 642.2 0 38 214 35 642.2 0 35 215 25 642.2 0 25 216 33 642.2 0 33 217 118 642.2 0 118 218 87 642.2 0 87 219 189 642.2 0 189 220 27 642.2 0 27 221 79 642.2 0 79 222 498 642.2 0 498 223 746 642.2 0 746 224 851 642.2 0 851 225 96 642.2 0 96 226 60 642.2 0 60 227 48 642.2 0 48 228 67 642.2 0 67 229 61 642.2 0 61 230 36 642.2 0 36 231 27 642.2 0 27 232 15 642.2 0 15 233 28 642.2 0 28 234 25 642.2 0 25 235 35 642.2 0 35 236 27 642.2 0 27 237 37 642.2 0 37 238 56 642.2 0 56 239 82 642.2 0 82 240 77 642.2 0 77 241 60 642.2 0 60 242 43 642.2 0 43 243 113 642.2 0 113 244 24 642.2 0 24 245 26 642.2 0 26 246 19 642.2 0 19 247 21 642.2 0 21 248 40 642.2 0 40 249 34 642.2 0 34 250 23 642.2 0 23 251 41 642.2 0 41 252 29 642.2 0 29 253 51 642.2 0 51 254 30 642.2 0 30 255 119 642.2 0 119 256 142 642.2 0 142 257 140 642.2 0 140 258 201 642.2 0 201 259 111 642.2 0 111 260 80 642.2 0 80 261 50 642.2 0 50 262 67 642.2 0 67 263 46 642.2 0 46 264 85 642.2 0 85 265 30 642.2 0 30 266 48 642.2 0 48 267 36 642.2 0 36 268 35 642.2 0 35 269 39 642.2 0 39 270 34 642.2 0 34 271 53 642.2 0 53 272 42 642.2 0 42 273 70 642.2 0 70 274 31 642.2 0 31 275 37 642.2 0 37 276 54 642.2 0 54 277 111 642.2 0 111 278 137 642.2 0 137 279 107 642.2 0 107 280 81 642.2 0 81 281 42 642.2 0 42 282 46 642.2 0 46 283 46 642.2 0 46 284 69 642.2 0 69 285 64 642.2 0 64 286 40 642.2 0 40 287 59 642.2 0 59 288 51 642.2 0 51 289 86 642.2 0 86 290 71 642.2 0 71 291 129 642.2 0 129 292 76 642.2 0 76 293 53 642.2 0 53 294 56 642.2 0 56 295 100 642.2 0 100 296 59 642.2 0 59 297 83 642.2 0 83 298 73 642.2 0 73 299 97 642.2 0 97 300 83 642.2 0 83 301 80 642.2 0 80 Finished in 1.14 s (44 us/read; 1.38 M reads/minute). === Summary === Total reads processed: 26,198 Reads with adapters: 6,084 (23.2%) Reads that were too short: 62 (0.2%) Reads written (passing filters): 6,022 (23.0%) Total basepairs processed: 6,030,384 bp Total written (filtered): 1,653,594 bp (27.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 6084 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 7 0.0 3 0 0 1 6 16 27 0.0 3 0 22 1 4 17 2522 0.0 3 2295 186 31 10 18 3341 0.0 3 3086 205 40 10 19 16 0.0 3 2 5 3 6 20 15 0.0 3 0 0 4 11 21 19 0.0 3 0 0 0 19 29 1 0.0 3 0 0 0 1 31 2 0.0 3 0 0 0 2 34 3 0.0 3 0 0 0 3 35 1 0.0 3 0 0 0 1 36 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 42 2 0.0 3 0 0 0 2 43 3 0.0 3 0 0 0 3 44 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 47 2 0.0 3 0 0 0 2 48 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 63 2 0.0 3 0 0 0 2 70 2 0.0 3 0 0 0 2 71 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 75 2 0.0 3 0 0 1 1 82 4 0.0 3 0 0 0 4 85 2 0.0 3 0 0 0 2 88 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 90 3 0.0 3 0 0 0 3 93 1 0.0 3 0 0 0 1 94 2 0.0 3 0 0 0 2 96 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 99 2 0.0 3 0 0 0 2 100 2 0.0 3 0 0 0 2 101 5 0.0 3 0 0 0 5 102 4 0.0 3 0 0 0 4 103 2 0.0 3 0 0 0 2 104 1 0.0 3 0 0 1 105 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 111 3 0.0 3 0 0 0 3 112 4 0.0 3 0 0 0 4 114 1 0.0 3 0 0 0 1 116 2 0.0 3 0 0 0 2 118 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 133 2 0.0 3 0 0 0 2 134 4 0.0 3 0 0 0 4 135 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 146 2 0.0 3 0 0 0 2 156 2 0.0 3 0 0 0 2 158 4 0.0 3 0 0 1 3 159 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 188 6 0.0 3 0 0 0 6 189 2 0.0 3 0 0 0 2 192 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 0 1 199 1 0.0 3 0 0 0 1 204 1 0.0 3 0 0 0 1 209 1 0.0 3 0 0 0 1 212 1 0.0 3 0 0 0 1 215 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 0 1 233 1 0.0 3 0 0 0 1 235 2 0.0 3 0 0 0 2 264 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.21 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 6,022 Reads with adapters: 11 (0.2%) Reads written (passing filters): 6,022 (100.0%) Total basepairs processed: 1,653,594 bp Total written (filtered): 1,653,558 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 11 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 18.2% C: 36.4% G: 45.5% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 8 94.1 0 8 4 3 23.5 0 3