This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGATC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 68.44 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 22,835 (0.9%) Reads that were too short: 2,806 (0.1%) Reads written (passing filters): 20,029 (0.8%) Total basepairs processed: 791,719,999 bp Total written (filtered): 5,042,899 bp (0.6%) === Adapter 1 === Sequence: GTGATC; Type: regular 5'; Length: 6; Trimmed: 22835 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3322 642.2 0 3322 7 4341 642.2 0 4341 8 2266 642.2 0 2266 9 2887 642.2 0 2887 10 12 642.2 0 12 11 1 642.2 0 1 13 3 642.2 0 3 14 2 642.2 0 2 15 3 642.2 0 3 16 2 642.2 0 2 17 1 642.2 0 1 18 4 642.2 0 4 19 1 642.2 0 1 20 3 642.2 0 3 21 3 642.2 0 3 22 2 642.2 0 2 23 5 642.2 0 5 25 3 642.2 0 3 26 3 642.2 0 3 27 4 642.2 0 4 28 1 642.2 0 1 29 1 642.2 0 1 30 13 642.2 0 13 31 4 642.2 0 4 32 4 642.2 0 4 33 1 642.2 0 1 34 6 642.2 0 6 35 15 642.2 0 15 36 78 642.2 0 78 37 3 642.2 0 3 38 23 642.2 0 23 39 27 642.2 0 27 40 11 642.2 0 11 41 9 642.2 0 9 42 2 642.2 0 2 43 5 642.2 0 5 44 27 642.2 0 27 45 6 642.2 0 6 46 22 642.2 0 22 47 6 642.2 0 6 48 2 642.2 0 2 49 4 642.2 0 4 50 3 642.2 0 3 51 11 642.2 0 11 52 9 642.2 0 9 53 11 642.2 0 11 54 5 642.2 0 5 55 7 642.2 0 7 56 6 642.2 0 6 57 9 642.2 0 9 58 19 642.2 0 19 59 8 642.2 0 8 60 39 642.2 0 39 61 4 642.2 0 4 62 5 642.2 0 5 63 10 642.2 0 10 64 7 642.2 0 7 65 2 642.2 0 2 66 7 642.2 0 7 67 8 642.2 0 8 68 4 642.2 0 4 69 7 642.2 0 7 70 4 642.2 0 4 71 45 642.2 0 45 72 56 642.2 0 56 73 8 642.2 0 8 74 3 642.2 0 3 75 5 642.2 0 5 76 79 642.2 0 79 77 66 642.2 0 66 78 156 642.2 0 156 79 12 642.2 0 12 80 61 642.2 0 61 81 213 642.2 0 213 82 43 642.2 0 43 83 6 642.2 0 6 84 3 642.2 0 3 85 9 642.2 0 9 86 10 642.2 0 10 87 4 642.2 0 4 88 5 642.2 0 5 89 15 642.2 0 15 90 5 642.2 0 5 91 7 642.2 0 7 92 2 642.2 0 2 93 13 642.2 0 13 94 3 642.2 0 3 95 15 642.2 0 15 96 3 642.2 0 3 97 2 642.2 0 2 98 14 642.2 0 14 99 24 642.2 0 24 100 529 642.2 0 529 101 530 642.2 0 530 102 242 642.2 0 242 103 8 642.2 0 8 104 6 642.2 0 6 105 8 642.2 0 8 106 5 642.2 0 5 107 8 642.2 0 8 108 9 642.2 0 9 109 35 642.2 0 35 110 564 642.2 0 564 111 22 642.2 0 22 112 12 642.2 0 12 113 29 642.2 0 29 114 10 642.2 0 10 115 12 642.2 0 12 116 135 642.2 0 135 117 8 642.2 0 8 118 6 642.2 0 6 119 11 642.2 0 11 120 7 642.2 0 7 121 8 642.2 0 8 122 4 642.2 0 4 123 22 642.2 0 22 124 702 642.2 0 702 125 7 642.2 0 7 126 5 642.2 0 5 127 8 642.2 0 8 128 55 642.2 0 55 129 2 642.2 0 2 130 2 642.2 0 2 131 11 642.2 0 11 132 7 642.2 0 7 133 8 642.2 0 8 134 7 642.2 0 7 135 6 642.2 0 6 136 3 642.2 0 3 137 6 642.2 0 6 138 4 642.2 0 4 139 6 642.2 0 6 140 19 642.2 0 19 141 7 642.2 0 7 142 10 642.2 0 10 143 11 642.2 0 11 144 17 642.2 0 17 145 8 642.2 0 8 146 18 642.2 0 18 147 23 642.2 0 23 148 23 642.2 0 23 149 54 642.2 0 54 150 39 642.2 0 39 151 1225 642.2 0 1225 152 42 642.2 0 42 153 32 642.2 0 32 154 11 642.2 0 11 155 2 642.2 0 2 156 8 642.2 0 8 157 22 642.2 0 22 158 23 642.2 0 23 159 16 642.2 0 16 160 4 642.2 0 4 161 8 642.2 0 8 162 9 642.2 0 9 163 6 642.2 0 6 164 5 642.2 0 5 165 6 642.2 0 6 166 6 642.2 0 6 167 4 642.2 0 4 168 26 642.2 0 26 169 16 642.2 0 16 170 121 642.2 0 121 171 8 642.2 0 8 172 6 642.2 0 6 173 8 642.2 0 8 174 6 642.2 0 6 175 6 642.2 0 6 176 8 642.2 0 8 177 9 642.2 0 9 178 9 642.2 0 9 179 6 642.2 0 6 180 3 642.2 0 3 181 11 642.2 0 11 182 25 642.2 0 25 183 54 642.2 0 54 184 17 642.2 0 17 185 13 642.2 0 13 186 4 642.2 0 4 187 5 642.2 0 5 188 11 642.2 0 11 189 11 642.2 0 11 190 19 642.2 0 19 191 65 642.2 0 65 192 7 642.2 0 7 193 9 642.2 0 9 194 31 642.2 0 31 195 9 642.2 0 9 196 13 642.2 0 13 197 40 642.2 0 40 198 73 642.2 0 73 199 322 642.2 0 322 200 86 642.2 0 86 201 35 642.2 0 35 202 37 642.2 0 37 203 28 642.2 0 28 204 80 642.2 0 80 205 21 642.2 0 21 206 19 642.2 0 19 207 8 642.2 0 8 208 12 642.2 0 12 209 20 642.2 0 20 210 14 642.2 0 14 211 14 642.2 0 14 212 13 642.2 0 13 213 7 642.2 0 7 214 17 642.2 0 17 215 22 642.2 0 22 216 7 642.2 0 7 217 28 642.2 0 28 218 28 642.2 0 28 219 51 642.2 0 51 220 13 642.2 0 13 221 28 642.2 0 28 222 80 642.2 0 80 223 219 642.2 0 219 224 346 642.2 0 346 225 25 642.2 0 25 226 42 642.2 0 42 227 9 642.2 0 9 228 57 642.2 0 57 229 17 642.2 0 17 230 16 642.2 0 16 231 14 642.2 0 14 232 4 642.2 0 4 233 9 642.2 0 9 234 11 642.2 0 11 235 11 642.2 0 11 236 8 642.2 0 8 237 13 642.2 0 13 238 12 642.2 0 12 239 34 642.2 0 34 240 18 642.2 0 18 241 27 642.2 0 27 242 19 642.2 0 19 243 31 642.2 0 31 244 16 642.2 0 16 245 13 642.2 0 13 246 11 642.2 0 11 247 13 642.2 0 13 248 11 642.2 0 11 249 10 642.2 0 10 250 14 642.2 0 14 251 18 642.2 0 18 252 8 642.2 0 8 253 15 642.2 0 15 254 10 642.2 0 10 255 21 642.2 0 21 256 27 642.2 0 27 257 33 642.2 0 33 258 53 642.2 0 53 259 71 642.2 0 71 260 27 642.2 0 27 261 13 642.2 0 13 262 19 642.2 0 19 263 14 642.2 0 14 264 36 642.2 0 36 265 9 642.2 0 9 266 10 642.2 0 10 267 11 642.2 0 11 268 14 642.2 0 14 269 21 642.2 0 21 270 13 642.2 0 13 271 14 642.2 0 14 272 14 642.2 0 14 273 23 642.2 0 23 274 11 642.2 0 11 275 8 642.2 0 8 276 19 642.2 0 19 277 12 642.2 0 12 278 27 642.2 0 27 279 21 642.2 0 21 280 24 642.2 0 24 281 7 642.2 0 7 282 18 642.2 0 18 283 15 642.2 0 15 284 25 642.2 0 25 285 16 642.2 0 16 286 12 642.2 0 12 287 19 642.2 0 19 288 28 642.2 0 28 289 36 642.2 0 36 290 23 642.2 0 23 291 55 642.2 0 55 292 37 642.2 0 37 293 23 642.2 0 23 294 22 642.2 0 22 295 84 642.2 0 84 296 34 642.2 0 34 297 28 642.2 0 28 298 27 642.2 0 27 299 48 642.2 0 48 300 39 642.2 0 39 301 17 642.2 0 17 Finished in 1.03 s (51 us/read; 1.17 M reads/minute). === Summary === Total reads processed: 20,029 Reads with adapters: 7,430 (37.1%) Reads that were too short: 6 (0.0%) Reads written (passing filters): 7,424 (37.1%) Total basepairs processed: 5,042,899 bp Total written (filtered): 2,016,935 bp (40.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 7430 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 1 0.0 3 0 0 1 19 8 0.0 3 0 8 20 3008 0.0 4 2947 58 2 1 21 4321 0.0 4 4242 74 4 1 22 9 0.0 4 1 8 23 8 0.0 4 1 0 1 6 24 3 0.0 4 0 0 0 3 26 1 0.0 4 1 47 1 0.0 4 0 0 0 0 1 52 1 0.0 4 0 0 0 0 1 76 1 0.0 4 0 0 0 0 1 99 1 0.0 4 0 0 0 0 1 128 62 0.0 4 0 0 0 0 62 144 1 0.0 4 1 154 1 0.0 4 0 0 0 0 1 190 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.25 s (34 us/read; 1.78 M reads/minute). === Summary === Total reads processed: 7,424 Reads with adapters: 771 (10.4%) Reads written (passing filters): 7,424 (100.0%) Total basepairs processed: 2,016,935 bp Total written (filtered): 2,008,770 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 771 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.3% C: 2.1% G: 94.8% T: 1.8% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 5 116.0 0 5 4 2 29.0 0 2 5 1 7.2 0 1 6 1 1.8 0 1 9 107 0.0 0 3 104 10 244 0.0 1 7 237 11 408 0.0 1 2 406 12 1 0.0 1 0 1 19 1 0.0 1 1 209 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.