This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATTCTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 68.26 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 246,093 (9.4%) Reads that were too short: 5,534 (0.2%) Reads written (passing filters): 240,559 (9.1%) Total basepairs processed: 791,719,999 bp Total written (filtered): 43,056,828 bp (5.4%) === Adapter 1 === Sequence: ATTCTA; Type: regular 5'; Length: 6; Trimmed: 246093 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 460 642.2 0 460 7 18824 642.2 0 18824 8 18431 642.2 0 18431 9 10 642.2 0 10 10 6 642.2 0 6 11 53 642.2 0 53 12 34 642.2 0 34 13 40 642.2 0 40 14 9 642.2 0 9 15 16 642.2 0 16 16 12 642.2 0 12 17 11 642.2 0 11 18 8 642.2 0 8 19 7 642.2 0 7 20 17 642.2 0 17 21 24 642.2 0 24 22 15 642.2 0 15 23 9 642.2 0 9 24 8 642.2 0 8 25 27 642.2 0 27 26 48 642.2 0 48 27 40 642.2 0 40 28 16 642.2 0 16 29 12 642.2 0 12 30 9 642.2 0 9 31 23 642.2 0 23 32 17 642.2 0 17 33 22 642.2 0 22 34 17 642.2 0 17 35 13 642.2 0 13 36 24 642.2 0 24 37 29 642.2 0 29 38 35 642.2 0 35 39 15 642.2 0 15 40 47 642.2 0 47 41 12 642.2 0 12 42 12 642.2 0 12 43 47 642.2 0 47 44 13 642.2 0 13 45 5 642.2 0 5 46 13 642.2 0 13 47 14 642.2 0 14 48 8 642.2 0 8 49 13 642.2 0 13 50 8 642.2 0 8 51 7 642.2 0 7 52 9 642.2 0 9 53 15 642.2 0 15 54 16 642.2 0 16 55 21 642.2 0 21 56 10 642.2 0 10 57 18 642.2 0 18 58 18 642.2 0 18 59 15 642.2 0 15 60 15 642.2 0 15 61 38 642.2 0 38 62 10 642.2 0 10 63 19 642.2 0 19 64 12 642.2 0 12 65 8 642.2 0 8 66 8 642.2 0 8 67 8 642.2 0 8 68 12 642.2 0 12 69 18 642.2 0 18 70 26 642.2 0 26 71 59 642.2 0 59 72 253 642.2 0 253 73 26 642.2 0 26 74 11 642.2 0 11 75 17 642.2 0 17 76 74 642.2 0 74 77 15 642.2 0 15 78 23 642.2 0 23 79 61 642.2 0 61 80 34 642.2 0 34 81 11 642.2 0 11 82 21 642.2 0 21 83 18 642.2 0 18 84 12 642.2 0 12 85 23 642.2 0 23 86 19 642.2 0 19 87 7 642.2 0 7 88 14 642.2 0 14 89 16 642.2 0 16 90 25 642.2 0 25 91 74 642.2 0 74 92 8 642.2 0 8 93 21 642.2 0 21 94 16 642.2 0 16 95 22 642.2 0 22 96 21 642.2 0 21 97 51 642.2 0 51 98 36 642.2 0 36 99 84 642.2 0 84 100 24 642.2 0 24 101 14 642.2 0 14 102 51 642.2 0 51 103 41 642.2 0 41 104 61 642.2 0 61 105 52 642.2 0 52 106 63 642.2 0 63 107 67 642.2 0 67 108 52 642.2 0 52 109 34 642.2 0 34 110 197 642.2 0 197 111 32 642.2 0 32 112 75 642.2 0 75 113 66 642.2 0 66 114 53 642.2 0 53 115 245 642.2 0 245 116 28 642.2 0 28 117 18 642.2 0 18 118 33 642.2 0 33 119 24 642.2 0 24 120 41 642.2 0 41 121 104 642.2 0 104 122 456 642.2 0 456 123 338 642.2 0 338 124 262 642.2 0 262 125 349 642.2 0 349 126 73 642.2 0 73 127 135 642.2 0 135 128 97 642.2 0 97 129 60 642.2 0 60 130 173 642.2 0 173 131 79 642.2 0 79 132 149 642.2 0 149 133 111 642.2 0 111 134 104 642.2 0 104 135 263 642.2 0 263 136 273 642.2 0 273 137 512 642.2 0 512 138 1111 642.2 0 1111 139 1177 642.2 0 1177 140 1654 642.2 0 1654 141 5192 642.2 0 5192 142 49997 642.2 0 49997 143 35022 642.2 0 35022 144 86314 642.2 0 86314 145 362 642.2 0 362 146 43 642.2 0 43 147 66 642.2 0 66 148 46 642.2 0 46 149 48 642.2 0 48 150 58 642.2 0 58 151 32 642.2 0 32 152 45 642.2 0 45 153 25 642.2 0 25 154 28 642.2 0 28 155 50 642.2 0 50 156 47 642.2 0 47 157 72 642.2 0 72 158 111 642.2 0 111 159 119 642.2 0 119 160 168 642.2 0 168 161 228 642.2 0 228 162 1984 642.2 0 1984 163 1995 642.2 0 1995 164 8540 642.2 0 8540 165 53 642.2 0 53 166 15 642.2 0 15 167 23 642.2 0 23 168 31 642.2 0 31 169 31 642.2 0 31 170 21 642.2 0 21 171 16 642.2 0 16 172 18 642.2 0 18 173 24 642.2 0 24 174 11 642.2 0 11 175 14 642.2 0 14 176 30 642.2 0 30 177 120 642.2 0 120 178 58 642.2 0 58 179 26 642.2 0 26 180 14 642.2 0 14 181 14 642.2 0 14 182 15 642.2 0 15 183 37 642.2 0 37 184 25 642.2 0 25 185 23 642.2 0 23 186 27 642.2 0 27 187 19 642.2 0 19 188 83 642.2 0 83 189 260 642.2 0 260 190 81 642.2 0 81 191 13 642.2 0 13 192 29 642.2 0 29 193 10 642.2 0 10 194 21 642.2 0 21 195 22 642.2 0 22 196 23 642.2 0 23 197 27 642.2 0 27 198 19 642.2 0 19 199 16 642.2 0 16 200 22 642.2 0 22 201 17 642.2 0 17 202 19 642.2 0 19 203 19 642.2 0 19 204 26 642.2 0 26 205 29 642.2 0 29 206 18 642.2 0 18 207 32 642.2 0 32 208 34 642.2 0 34 209 52 642.2 0 52 210 92 642.2 0 92 211 53 642.2 0 53 212 42 642.2 0 42 213 46 642.2 0 46 214 186 642.2 0 186 215 118 642.2 0 118 216 100 642.2 0 100 217 23 642.2 0 23 218 25 642.2 0 25 219 13 642.2 0 13 220 25 642.2 0 25 221 27 642.2 0 27 222 40 642.2 0 40 223 65 642.2 0 65 224 64 642.2 0 64 225 146 642.2 0 146 226 83 642.2 0 83 227 49 642.2 0 49 228 22 642.2 0 22 229 21 642.2 0 21 230 28 642.2 0 28 231 15 642.2 0 15 232 55 642.2 0 55 233 70 642.2 0 70 234 82 642.2 0 82 235 21 642.2 0 21 236 48 642.2 0 48 237 24 642.2 0 24 238 40 642.2 0 40 239 100 642.2 0 100 240 38 642.2 0 38 241 34 642.2 0 34 242 22 642.2 0 22 243 25 642.2 0 25 244 35 642.2 0 35 245 37 642.2 0 37 246 32 642.2 0 32 247 19 642.2 0 19 248 25 642.2 0 25 249 20 642.2 0 20 250 13 642.2 0 13 251 37 642.2 0 37 252 26 642.2 0 26 253 21 642.2 0 21 254 21 642.2 0 21 255 39 642.2 0 39 256 41 642.2 0 41 257 56 642.2 0 56 258 53 642.2 0 53 259 43 642.2 0 43 260 67 642.2 0 67 261 197 642.2 0 197 262 89 642.2 0 89 263 392 642.2 0 392 264 94 642.2 0 94 265 49 642.2 0 49 266 27 642.2 0 27 267 35 642.2 0 35 268 36 642.2 0 36 269 56 642.2 0 56 270 59 642.2 0 59 271 49 642.2 0 49 272 24 642.2 0 24 273 41 642.2 0 41 274 44 642.2 0 44 275 52 642.2 0 52 276 48 642.2 0 48 277 54 642.2 0 54 278 49 642.2 0 49 279 163 642.2 0 163 280 144 642.2 0 144 281 280 642.2 0 280 282 76 642.2 0 76 283 63 642.2 0 63 284 61 642.2 0 61 285 77 642.2 0 77 286 36 642.2 0 36 287 53 642.2 0 53 288 23 642.2 0 23 289 33 642.2 0 33 290 33 642.2 0 33 291 29 642.2 0 29 292 45 642.2 0 45 293 34 642.2 0 34 294 45 642.2 0 45 295 82 642.2 0 82 296 48 642.2 0 48 297 57 642.2 0 57 298 38 642.2 0 38 299 21 642.2 0 21 300 34 642.2 0 34 301 8 642.2 0 8 Finished in 8.10 s (34 us/read; 1.78 M reads/minute). === Summary === Total reads processed: 240,559 Reads with adapters: 18,808 (7.8%) Reads that were too short: 88 (0.0%) Reads written (passing filters): 18,720 (7.8%) Total basepairs processed: 43,056,828 bp Total written (filtered): 5,179,945 bp (12.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 18808 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 29 0.0 3 0 0 22 7 16 377 0.0 3 0 321 43 13 17 18118 0.0 3 16556 1276 219 67 18 65 0.0 3 7 40 8 10 19 66 0.0 3 1 2 26 37 20 25 0.0 3 0 0 0 25 26 1 0.0 3 0 0 0 1 29 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 49 3 0.0 3 0 0 0 3 50 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 59 13 0.0 3 0 0 0 13 60 1 0.0 3 0 0 0 1 69 2 0.0 3 0 0 0 2 72 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 77 1 0.0 3 1 81 1 0.0 3 0 0 1 82 2 0.0 3 0 0 0 2 83 2 0.0 3 0 0 0 2 86 1 0.0 3 0 0 0 1 88 2 0.0 3 0 0 0 2 90 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 108 3 0.0 3 0 0 0 3 109 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 127 2 0.0 3 0 0 0 2 128 3 0.0 3 0 0 1 2 142 1 0.0 3 0 0 0 1 144 4 0.0 3 0 0 0 4 145 6 0.0 3 0 0 1 5 146 12 0.0 3 0 0 0 12 147 7 0.0 3 0 0 0 7 148 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 1 153 1 0.0 3 0 0 0 1 154 2 0.0 3 0 0 0 2 156 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 1 164 2 0.0 3 0 0 0 2 166 1 0.0 3 0 0 0 1 167 2 0.0 3 0 0 0 2 182 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 212 1 0.0 3 0 0 0 1 213 1 0.0 3 0 0 0 1 229 1 0.0 3 0 0 0 1 233 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 1 244 1 0.0 3 0 0 0 1 256 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 282 4 0.0 3 0 0 1 3 283 1 0.0 3 0 0 0 1 289 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.64 s (34 us/read; 1.76 M reads/minute). === Summary === Total reads processed: 18,720 Reads with adapters: 38 (0.2%) Reads written (passing filters): 18,720 (100.0%) Total basepairs processed: 5,179,945 bp Total written (filtered): 5,179,803 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 38 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 28.9% C: 15.8% G: 47.4% T: 7.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 29 292.5 0 29 4 1 73.1 0 1 5 2 18.3 0 2 6 5 4.6 0 5 11 1 0.0 1 0 1