This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATTCTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 68.37 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 280,792 (10.7%) Reads that were too short: 3,717 (0.1%) Reads written (passing filters): 277,075 (10.5%) Total basepairs processed: 791,719,999 bp Total written (filtered): 49,169,922 bp (6.2%) === Adapter 1 === Sequence: ATTCTA; Type: regular 5'; Length: 6; Trimmed: 280792 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 440 642.2 0 440 7 18404 642.2 0 18404 8 21260 642.2 0 21260 9 17 642.2 0 17 10 12 642.2 0 12 11 18 642.2 0 18 12 16 642.2 0 16 13 14 642.2 0 14 14 9 642.2 0 9 15 13 642.2 0 13 16 5 642.2 0 5 17 5 642.2 0 5 18 12 642.2 0 12 19 8 642.2 0 8 20 13 642.2 0 13 21 20 642.2 0 20 22 9 642.2 0 9 23 5 642.2 0 5 24 11 642.2 0 11 25 7 642.2 0 7 26 10 642.2 0 10 27 24 642.2 0 24 28 13 642.2 0 13 29 10 642.2 0 10 30 10 642.2 0 10 31 13 642.2 0 13 32 7 642.2 0 7 33 11 642.2 0 11 34 9 642.2 0 9 35 9 642.2 0 9 36 7 642.2 0 7 37 8 642.2 0 8 38 3 642.2 0 3 39 6 642.2 0 6 40 9 642.2 0 9 41 9 642.2 0 9 42 10 642.2 0 10 43 47 642.2 0 47 44 11 642.2 0 11 45 5 642.2 0 5 46 16 642.2 0 16 47 6 642.2 0 6 48 6 642.2 0 6 49 6 642.2 0 6 50 1 642.2 0 1 51 4 642.2 0 4 52 5 642.2 0 5 53 8 642.2 0 8 54 12 642.2 0 12 55 13 642.2 0 13 56 5 642.2 0 5 57 22 642.2 0 22 58 13 642.2 0 13 59 14 642.2 0 14 60 11 642.2 0 11 61 42 642.2 0 42 62 1 642.2 0 1 63 19 642.2 0 19 64 15 642.2 0 15 65 7 642.2 0 7 66 9 642.2 0 9 67 11 642.2 0 11 68 13 642.2 0 13 69 18 642.2 0 18 70 14 642.2 0 14 71 29 642.2 0 29 72 296 642.2 0 296 73 20 642.2 0 20 74 11 642.2 0 11 75 19 642.2 0 19 76 47 642.2 0 47 77 8 642.2 0 8 78 9 642.2 0 9 79 73 642.2 0 73 80 22 642.2 0 22 81 10 642.2 0 10 82 26 642.2 0 26 83 14 642.2 0 14 84 14 642.2 0 14 85 11 642.2 0 11 86 20 642.2 0 20 87 17 642.2 0 17 88 15 642.2 0 15 89 33 642.2 0 33 90 23 642.2 0 23 91 102 642.2 0 102 92 23 642.2 0 23 93 22 642.2 0 22 94 16 642.2 0 16 95 36 642.2 0 36 96 18 642.2 0 18 97 50 642.2 0 50 98 36 642.2 0 36 99 61 642.2 0 61 100 25 642.2 0 25 101 18 642.2 0 18 102 37 642.2 0 37 103 35 642.2 0 35 104 41 642.2 0 41 105 70 642.2 0 70 106 62 642.2 0 62 107 60 642.2 0 60 108 46 642.2 0 46 109 31 642.2 0 31 110 175 642.2 0 175 111 30 642.2 0 30 112 57 642.2 0 57 113 28 642.2 0 28 114 43 642.2 0 43 115 272 642.2 0 272 116 15 642.2 0 15 117 14 642.2 0 14 118 33 642.2 0 33 119 15 642.2 0 15 120 34 642.2 0 34 121 53 642.2 0 53 122 382 642.2 0 382 123 221 642.2 0 221 124 202 642.2 0 202 125 398 642.2 0 398 126 62 642.2 0 62 127 129 642.2 0 129 128 96 642.2 0 96 129 60 642.2 0 60 130 176 642.2 0 176 131 102 642.2 0 102 132 148 642.2 0 148 133 135 642.2 0 135 134 114 642.2 0 114 135 222 642.2 0 222 136 280 642.2 0 280 137 555 642.2 0 555 138 1300 642.2 0 1300 139 1352 642.2 0 1352 140 1696 642.2 0 1696 141 4748 642.2 0 4748 142 60262 642.2 0 60262 143 43426 642.2 0 43426 144 106495 642.2 0 106495 145 414 642.2 0 414 146 40 642.2 0 40 147 47 642.2 0 47 148 28 642.2 0 28 149 33 642.2 0 33 150 44 642.2 0 44 151 25 642.2 0 25 152 25 642.2 0 25 153 21 642.2 0 21 154 14 642.2 0 14 155 24 642.2 0 24 156 23 642.2 0 23 157 47 642.2 0 47 158 82 642.2 0 82 159 67 642.2 0 67 160 107 642.2 0 107 161 170 642.2 0 170 162 1824 642.2 0 1824 163 1742 642.2 0 1742 164 5580 642.2 0 5580 165 21 642.2 0 21 166 9 642.2 0 9 167 15 642.2 0 15 168 17 642.2 0 17 169 30 642.2 0 30 170 18 642.2 0 18 171 12 642.2 0 12 172 16 642.2 0 16 173 8 642.2 0 8 174 11 642.2 0 11 175 19 642.2 0 19 176 27 642.2 0 27 177 83 642.2 0 83 178 30 642.2 0 30 179 16 642.2 0 16 180 12 642.2 0 12 181 7 642.2 0 7 182 18 642.2 0 18 183 17 642.2 0 17 184 19 642.2 0 19 185 24 642.2 0 24 186 15 642.2 0 15 187 18 642.2 0 18 188 77 642.2 0 77 189 305 642.2 0 305 190 65 642.2 0 65 191 11 642.2 0 11 192 21 642.2 0 21 193 12 642.2 0 12 194 11 642.2 0 11 195 18 642.2 0 18 196 17 642.2 0 17 197 12 642.2 0 12 198 14 642.2 0 14 199 13 642.2 0 13 200 10 642.2 0 10 201 14 642.2 0 14 202 19 642.2 0 19 203 12 642.2 0 12 204 16 642.2 0 16 205 31 642.2 0 31 206 21 642.2 0 21 207 18 642.2 0 18 208 27 642.2 0 27 209 52 642.2 0 52 210 69 642.2 0 69 211 26 642.2 0 26 212 31 642.2 0 31 213 30 642.2 0 30 214 80 642.2 0 80 215 63 642.2 0 63 216 69 642.2 0 69 217 21 642.2 0 21 218 15 642.2 0 15 219 8 642.2 0 8 220 16 642.2 0 16 221 21 642.2 0 21 222 25 642.2 0 25 223 25 642.2 0 25 224 29 642.2 0 29 225 121 642.2 0 121 226 36 642.2 0 36 227 43 642.2 0 43 228 20 642.2 0 20 229 11 642.2 0 11 230 14 642.2 0 14 231 22 642.2 0 22 232 24 642.2 0 24 233 34 642.2 0 34 234 45 642.2 0 45 235 24 642.2 0 24 236 31 642.2 0 31 237 28 642.2 0 28 238 35 642.2 0 35 239 94 642.2 0 94 240 29 642.2 0 29 241 20 642.2 0 20 242 19 642.2 0 19 243 21 642.2 0 21 244 29 642.2 0 29 245 32 642.2 0 32 246 32 642.2 0 32 247 22 642.2 0 22 248 14 642.2 0 14 249 22 642.2 0 22 250 14 642.2 0 14 251 11 642.2 0 11 252 18 642.2 0 18 253 20 642.2 0 20 254 17 642.2 0 17 255 32 642.2 0 32 256 44 642.2 0 44 257 33 642.2 0 33 258 32 642.2 0 32 259 37 642.2 0 37 260 38 642.2 0 38 261 100 642.2 0 100 262 61 642.2 0 61 263 265 642.2 0 265 264 57 642.2 0 57 265 22 642.2 0 22 266 20 642.2 0 20 267 18 642.2 0 18 268 23 642.2 0 23 269 40 642.2 0 40 270 33 642.2 0 33 271 25 642.2 0 25 272 24 642.2 0 24 273 22 642.2 0 22 274 24 642.2 0 24 275 34 642.2 0 34 276 44 642.2 0 44 277 27 642.2 0 27 278 30 642.2 0 30 279 107 642.2 0 107 280 89 642.2 0 89 281 195 642.2 0 195 282 44 642.2 0 44 283 44 642.2 0 44 284 37 642.2 0 37 285 34 642.2 0 34 286 32 642.2 0 32 287 26 642.2 0 26 288 27 642.2 0 27 289 21 642.2 0 21 290 23 642.2 0 23 291 15 642.2 0 15 292 24 642.2 0 24 293 23 642.2 0 23 294 31 642.2 0 31 295 55 642.2 0 55 296 40 642.2 0 40 297 57 642.2 0 57 298 26 642.2 0 26 299 25 642.2 0 25 300 16 642.2 0 16 301 15 642.2 0 15 Finished in 10.04 s (36 us/read; 1.66 M reads/minute). === Summary === Total reads processed: 277,075 Reads with adapters: 22,111 (8.0%) Reads that were too short: 14 (0.0%) Reads written (passing filters): 22,097 (8.0%) Total basepairs processed: 49,169,922 bp Total written (filtered): 6,003,899 bp (12.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 22111 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 4 0.0 3 0 0 4 19 58 0.0 3 0 54 3 0 1 20 21698 0.0 4 21382 306 10 21 49 0.0 4 6 42 1 22 3 0.0 4 0 0 3 23 13 0.0 4 1 0 0 12 24 1 0.0 4 0 0 0 0 1 37 6 0.0 4 0 0 0 0 6 38 6 0.0 4 0 0 0 0 6 42 1 0.0 4 0 1 61 1 0.0 4 0 0 0 0 1 66 1 0.0 4 1 81 2 0.0 4 0 0 0 0 2 91 1 0.0 4 0 1 126 2 0.0 4 0 0 0 0 2 127 254 0.0 4 0 0 0 0 254 128 1 0.0 4 0 0 0 0 1 182 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 209 1 0.0 4 0 0 0 0 1 228 2 0.0 4 0 0 0 0 2 229 2 0.0 4 0 0 0 0 2 288 1 0.0 4 0 0 0 0 1 292 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.77 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 22,097 Reads with adapters: 2,651 (12.0%) Reads written (passing filters): 22,097 (100.0%) Total basepairs processed: 6,003,899 bp Total written (filtered): 5,969,563 bp (99.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2651 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.9% C: 6.0% G: 90.3% T: 2.8% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 7 345.3 0 7 4 1 86.3 0 1 5 1 21.6 0 1 6 1 5.4 0 1 8 1 0.3 0 1 9 116 0.1 0 4 112 10 484 0.0 1 17 467 11 2009 0.0 1 11 1998 12 3 0.0 1 1 2 224 27 0.0 1 26 1 225 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.