This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACCTAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 70.32 s (27 us/read; 2.24 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 239,245 (9.1%) Reads that were too short: 25,191 (1.0%) Reads written (passing filters): 214,054 (8.1%) Total basepairs processed: 791,719,999 bp Total written (filtered): 58,629,318 bp (7.4%) === Adapter 1 === Sequence: ACCTAT; Type: regular 5'; Length: 6; Trimmed: 239245 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 795 642.2 0 795 7 19288 642.2 0 19288 8 973 642.2 0 973 9 16856 642.2 0 16856 10 763 642.2 0 763 11 1513 642.2 0 1513 12 16164 642.2 0 16164 13 112641 642.2 0 112641 14 16736 642.2 0 16736 15 88 642.2 0 88 16 63 642.2 0 63 17 75 642.2 0 75 18 73 642.2 0 73 19 40 642.2 0 40 20 44 642.2 0 44 21 83 642.2 0 83 22 80 642.2 0 80 23 57 642.2 0 57 24 69 642.2 0 69 25 69 642.2 0 69 26 98 642.2 0 98 27 237 642.2 0 237 28 95 642.2 0 95 29 63 642.2 0 63 30 69 642.2 0 69 31 63 642.2 0 63 32 53 642.2 0 53 33 62 642.2 0 62 34 62 642.2 0 62 35 65 642.2 0 65 36 57 642.2 0 57 37 51 642.2 0 51 38 122 642.2 0 122 39 66 642.2 0 66 40 120 642.2 0 120 41 64 642.2 0 64 42 76 642.2 0 76 43 187 642.2 0 187 44 208 642.2 0 208 45 115 642.2 0 115 46 53 642.2 0 53 47 48 642.2 0 48 48 73 642.2 0 73 49 83 642.2 0 83 50 52 642.2 0 52 51 55 642.2 0 55 52 45 642.2 0 45 53 75 642.2 0 75 54 45 642.2 0 45 55 51 642.2 0 51 56 60 642.2 0 60 57 80 642.2 0 80 58 78 642.2 0 78 59 59 642.2 0 59 60 52 642.2 0 52 61 58 642.2 0 58 62 41 642.2 0 41 63 68 642.2 0 68 64 50 642.2 0 50 65 78 642.2 0 78 66 80 642.2 0 80 67 102 642.2 0 102 68 57 642.2 0 57 69 58 642.2 0 58 70 64 642.2 0 64 71 54 642.2 0 54 72 51 642.2 0 51 73 75 642.2 0 75 74 71 642.2 0 71 75 62 642.2 0 62 76 61 642.2 0 61 77 64 642.2 0 64 78 87 642.2 0 87 79 69 642.2 0 69 80 72 642.2 0 72 81 61 642.2 0 61 82 52 642.2 0 52 83 68 642.2 0 68 84 73 642.2 0 73 85 79 642.2 0 79 86 69 642.2 0 69 87 73 642.2 0 73 88 52 642.2 0 52 89 94 642.2 0 94 90 326 642.2 0 326 91 91 642.2 0 91 92 154 642.2 0 154 93 79 642.2 0 79 94 75 642.2 0 75 95 75 642.2 0 75 96 62 642.2 0 62 97 77 642.2 0 77 98 76 642.2 0 76 99 57 642.2 0 57 100 92 642.2 0 92 101 67 642.2 0 67 102 94 642.2 0 94 103 71 642.2 0 71 104 411 642.2 0 411 105 177 642.2 0 177 106 660 642.2 0 660 107 119 642.2 0 119 108 198 642.2 0 198 109 110 642.2 0 110 110 149 642.2 0 149 111 110 642.2 0 110 112 128 642.2 0 128 113 99 642.2 0 99 114 128 642.2 0 128 115 138 642.2 0 138 116 94 642.2 0 94 117 86 642.2 0 86 118 79 642.2 0 79 119 53 642.2 0 53 120 83 642.2 0 83 121 79 642.2 0 79 122 67 642.2 0 67 123 75 642.2 0 75 124 122 642.2 0 122 125 108 642.2 0 108 126 325 642.2 0 325 127 754 642.2 0 754 128 449 642.2 0 449 129 239 642.2 0 239 130 80 642.2 0 80 131 68 642.2 0 68 132 65 642.2 0 65 133 119 642.2 0 119 134 218 642.2 0 218 135 150 642.2 0 150 136 91 642.2 0 91 137 128 642.2 0 128 138 676 642.2 0 676 139 854 642.2 0 854 140 1680 642.2 0 1680 141 591 642.2 0 591 142 209 642.2 0 209 143 99 642.2 0 99 144 84 642.2 0 84 145 112 642.2 0 112 146 110 642.2 0 110 147 102 642.2 0 102 148 79 642.2 0 79 149 76 642.2 0 76 150 76 642.2 0 76 151 304 642.2 0 304 152 251 642.2 0 251 153 398 642.2 0 398 154 212 642.2 0 212 155 147 642.2 0 147 156 113 642.2 0 113 157 112 642.2 0 112 158 302 642.2 0 302 159 558 642.2 0 558 160 2494 642.2 0 2494 161 988 642.2 0 988 162 213 642.2 0 213 163 528 642.2 0 528 164 101 642.2 0 101 165 77 642.2 0 77 166 70 642.2 0 70 167 117 642.2 0 117 168 123 642.2 0 123 169 170 642.2 0 170 170 83 642.2 0 83 171 103 642.2 0 103 172 87 642.2 0 87 173 80 642.2 0 80 174 136 642.2 0 136 175 116 642.2 0 116 176 118 642.2 0 118 177 100 642.2 0 100 178 78 642.2 0 78 179 75 642.2 0 75 180 84 642.2 0 84 181 148 642.2 0 148 182 296 642.2 0 296 183 139 642.2 0 139 184 114 642.2 0 114 185 91 642.2 0 91 186 143 642.2 0 143 187 88 642.2 0 88 188 193 642.2 0 193 189 129 642.2 0 129 190 146 642.2 0 146 191 51 642.2 0 51 192 79 642.2 0 79 193 61 642.2 0 61 194 73 642.2 0 73 195 68 642.2 0 68 196 77 642.2 0 77 197 82 642.2 0 82 198 69 642.2 0 69 199 108 642.2 0 108 200 97 642.2 0 97 201 106 642.2 0 106 202 98 642.2 0 98 203 87 642.2 0 87 204 73 642.2 0 73 205 91 642.2 0 91 206 70 642.2 0 70 207 102 642.2 0 102 208 98 642.2 0 98 209 103 642.2 0 103 210 60 642.2 0 60 211 96 642.2 0 96 212 81 642.2 0 81 213 142 642.2 0 142 214 272 642.2 0 272 215 228 642.2 0 228 216 163 642.2 0 163 217 120 642.2 0 120 218 119 642.2 0 119 219 96 642.2 0 96 220 291 642.2 0 291 221 442 642.2 0 442 222 299 642.2 0 299 223 150 642.2 0 150 224 225 642.2 0 225 225 132 642.2 0 132 226 319 642.2 0 319 227 81 642.2 0 81 228 75 642.2 0 75 229 73 642.2 0 73 230 106 642.2 0 106 231 147 642.2 0 147 232 192 642.2 0 192 233 483 642.2 0 483 234 568 642.2 0 568 235 197 642.2 0 197 236 198 642.2 0 198 237 82 642.2 0 82 238 77 642.2 0 77 239 71 642.2 0 71 240 131 642.2 0 131 241 185 642.2 0 185 242 190 642.2 0 190 243 143 642.2 0 143 244 117 642.2 0 117 245 64 642.2 0 64 246 59 642.2 0 59 247 99 642.2 0 99 248 103 642.2 0 103 249 63 642.2 0 63 250 96 642.2 0 96 251 91 642.2 0 91 252 95 642.2 0 95 253 154 642.2 0 154 254 129 642.2 0 129 255 82 642.2 0 82 256 77 642.2 0 77 257 101 642.2 0 101 258 87 642.2 0 87 259 75 642.2 0 75 260 129 642.2 0 129 261 129 642.2 0 129 262 146 642.2 0 146 263 117 642.2 0 117 264 96 642.2 0 96 265 89 642.2 0 89 266 98 642.2 0 98 267 225 642.2 0 225 268 211 642.2 0 211 269 348 642.2 0 348 270 149 642.2 0 149 271 100 642.2 0 100 272 100 642.2 0 100 273 102 642.2 0 102 274 88 642.2 0 88 275 105 642.2 0 105 276 134 642.2 0 134 277 452 642.2 0 452 278 382 642.2 0 382 279 352 642.2 0 352 280 711 642.2 0 711 281 2993 642.2 0 2993 282 3188 642.2 0 3188 283 2551 642.2 0 2551 284 737 642.2 0 737 285 112 642.2 0 112 286 160 642.2 0 160 287 168 642.2 0 168 288 488 642.2 0 488 289 221 642.2 0 221 290 661 642.2 0 661 291 176 642.2 0 176 292 107 642.2 0 107 293 91 642.2 0 91 294 130 642.2 0 130 295 147 642.2 0 147 296 137 642.2 0 137 297 77 642.2 0 77 298 94 642.2 0 94 299 72 642.2 0 72 300 69 642.2 0 69 301 101 642.2 0 101 Finished in 9.43 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 214,054 Reads with adapters: 22,776 (10.6%) Reads that were too short: 689 (0.3%) Reads written (passing filters): 22,087 (10.3%) Total basepairs processed: 58,629,318 bp Total written (filtered): 5,627,526 bp (9.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 22776 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 10 0.0 2 0 0 0 10 15 12 0.0 3 0 0 5 7 16 182 0.0 3 0 141 20 21 17 18273 0.0 3 16577 1340 278 78 18 41 0.0 3 0 31 6 4 19 63 0.0 3 1 1 30 31 20 177 0.0 3 135 8 2 32 21 2 0.0 3 0 0 0 2 22 2 0.0 3 0 0 0 2 23 1 0.0 3 0 0 0 1 25 2 0.0 3 0 0 0 2 29 2 0.0 3 0 0 0 2 31 21 0.0 3 1 0 3 17 32 21 0.0 3 0 0 0 21 35 7 0.0 3 0 0 0 7 36 5 0.0 3 0 0 0 5 37 4 0.0 3 0 0 0 4 39 12 0.0 3 0 0 0 12 40 4 0.0 3 0 1 0 3 41 3 0.0 3 0 0 1 2 42 16 0.0 3 0 0 0 16 43 22 0.0 3 0 0 1 21 44 1 0.0 3 0 0 0 1 45 6 0.0 3 0 0 0 6 46 3 0.0 3 0 0 0 3 47 3 0.0 3 0 0 0 3 48 2 0.0 3 0 0 0 2 49 2 0.0 3 0 0 0 2 50 2 0.0 3 0 0 0 2 51 3 0.0 3 0 0 0 3 53 89 0.0 3 0 0 2 87 54 4 0.0 3 0 0 0 4 56 12 0.0 3 0 0 0 12 57 8 0.0 3 0 0 0 8 58 1 0.0 3 0 0 1 59 9 0.0 3 0 0 0 9 60 2 0.0 3 2 61 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 65 7 0.0 3 0 0 0 7 66 3 0.0 3 0 0 0 3 67 32 0.0 3 0 0 0 32 68 1 0.0 3 0 0 0 1 69 4 0.0 3 0 0 0 4 70 12 0.0 3 0 0 0 12 71 4 0.0 3 0 0 0 4 73 2 0.0 3 0 0 0 2 76 3 0.0 3 0 0 0 3 77 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 83 2 0.0 3 0 0 0 2 84 16 0.0 3 0 0 0 16 85 2 0.0 3 0 0 0 2 86 1 0.0 3 0 0 0 1 87 8 0.0 3 1 0 0 7 88 49 0.0 3 0 0 1 48 89 2 0.0 3 0 0 0 2 90 5 0.0 3 0 0 0 5 91 4 0.0 3 0 0 0 4 92 2 0.0 3 0 0 0 2 93 3 0.0 3 0 0 0 3 94 1 0.0 3 0 0 0 1 95 2 0.0 3 0 0 0 2 96 2 0.0 3 0 0 0 2 97 1 0.0 3 0 0 0 1 99 5 0.0 3 0 0 2 3 100 1 0.0 3 0 0 0 1 101 118 0.0 3 0 0 104 14 102 3 0.0 3 0 0 1 2 103 1 0.0 3 0 0 0 1 104 3 0.0 3 0 0 0 3 105 3 0.0 3 0 0 0 3 106 1 0.0 3 0 0 0 1 107 2 0.0 3 0 0 0 2 108 5 0.0 3 0 0 0 5 109 9 0.0 3 0 0 0 9 110 127 0.0 3 0 0 0 127 111 12 0.0 3 0 0 1 11 112 11 0.0 3 0 0 0 11 113 3 0.0 3 0 0 0 3 114 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 118 11 0.0 3 0 0 0 11 119 2 0.0 3 0 0 0 2 120 5 0.0 3 0 0 0 5 121 1 0.0 3 0 0 0 1 122 3 0.0 3 0 0 0 3 123 1 0.0 3 0 0 0 1 124 6 0.0 3 0 0 0 6 125 2 0.0 3 0 0 0 2 126 1 0.0 3 0 0 0 1 127 2 0.0 3 0 0 0 2 128 1 0.0 3 0 0 0 1 129 24 0.0 3 0 0 0 24 130 1 0.0 3 0 0 0 1 131 2 0.0 3 0 0 0 2 132 2 0.0 3 0 0 1 1 133 7 0.0 3 0 0 0 7 134 1 0.0 3 0 0 0 1 135 3 0.0 3 0 0 0 3 136 2 0.0 3 0 0 0 2 137 4 0.0 3 0 0 0 4 138 3 0.0 3 0 0 0 3 139 3 0.0 3 0 0 0 3 140 4 0.0 3 0 0 0 4 141 1 0.0 3 0 0 0 1 144 2 0.0 3 0 0 0 2 145 2 0.0 3 0 0 0 2 146 3 0.0 3 0 0 1 2 147 1 0.0 3 0 0 0 1 148 4 0.0 3 0 0 0 4 149 3 0.0 3 0 0 0 3 150 1 0.0 3 0 0 0 1 151 7 0.0 3 1 0 0 6 152 20 0.0 3 0 0 1 19 153 4 0.0 3 0 0 0 4 154 34 0.0 3 0 0 2 32 155 2 0.0 3 0 0 0 2 156 11 0.0 3 0 0 0 11 157 17 0.0 3 0 0 0 17 158 11 0.0 3 0 0 0 11 159 464 0.0 3 0 0 7 457 160 54 0.0 3 0 0 0 54 161 93 0.0 3 0 0 1 92 162 59 0.0 3 0 0 0 59 163 12 0.0 3 0 1 0 11 164 21 0.0 3 0 0 1 20 165 4 0.0 3 0 0 0 4 166 7 0.0 3 0 0 0 7 167 3 0.0 3 0 0 1 2 168 10 0.0 3 0 0 1 9 169 3 0.0 3 0 0 0 3 170 8 0.0 3 0 0 0 8 171 5 0.0 3 0 0 0 5 172 2 0.0 3 1 1 173 6 0.0 3 0 0 0 6 174 9 0.0 3 0 0 0 9 175 3 0.0 3 0 0 0 3 176 10 0.0 3 0 0 1 9 177 8 0.0 3 0 0 0 8 178 30 0.0 3 0 0 0 30 179 1174 0.0 3 0 0 15 1159 180 184 0.0 3 0 0 0 184 181 18 0.0 3 1 0 0 17 182 129 0.0 3 0 0 1 128 183 36 0.0 3 1 0 4 31 184 5 0.0 3 0 0 1 4 185 11 0.0 3 0 0 1 10 186 6 0.0 3 0 0 0 6 187 4 0.0 3 0 0 0 4 188 53 0.0 3 0 0 0 53 189 1 0.0 3 0 0 0 1 190 1 0.0 3 0 1 191 3 0.0 3 0 0 0 3 192 3 0.0 3 0 0 0 3 193 1 0.0 3 0 0 0 1 194 2 0.0 3 0 0 0 2 196 4 0.0 3 0 0 0 4 197 2 0.0 3 0 0 0 2 198 2 0.0 3 0 0 0 2 199 3 0.0 3 0 0 0 3 200 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 202 2 0.0 3 0 0 1 1 203 3 0.0 3 0 0 0 3 204 8 0.0 3 0 0 0 8 205 22 0.0 3 0 0 2 20 206 4 0.0 3 0 0 0 4 207 2 0.0 3 0 0 0 2 208 3 0.0 3 0 0 0 3 209 2 0.0 3 0 0 0 2 210 8 0.0 3 0 1 0 7 211 4 0.0 3 1 0 0 3 212 8 0.0 3 0 0 0 8 213 2 0.0 3 0 0 0 2 214 6 0.0 3 0 0 0 6 216 4 0.0 3 1 0 0 3 217 4 0.0 3 0 0 1 3 218 3 0.0 3 1 0 0 2 219 2 0.0 3 0 0 0 2 220 2 0.0 3 0 0 0 2 221 5 0.0 3 0 0 0 5 222 5 0.0 3 0 0 0 5 223 4 0.0 3 0 0 0 4 224 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 227 1 0.0 3 0 0 0 1 228 5 0.0 3 0 0 0 5 229 4 0.0 3 0 0 0 4 230 17 0.0 3 0 0 1 16 231 9 0.0 3 0 0 0 9 232 5 0.0 3 0 1 0 4 233 1 0.0 3 0 0 0 1 234 5 0.0 3 0 0 1 4 235 3 0.0 3 0 0 0 3 236 1 0.0 3 0 0 0 1 237 3 0.0 3 0 0 0 3 238 2 0.0 3 0 0 0 2 239 11 0.0 3 0 0 0 11 240 1 0.0 3 0 0 0 1 242 2 0.0 3 0 0 1 1 243 2 0.0 3 0 0 0 2 244 1 0.0 3 0 0 0 1 245 2 0.0 3 0 0 0 2 246 16 0.0 3 0 0 0 16 249 2 0.0 3 0 0 0 2 251 5 0.0 3 0 0 0 5 252 7 0.0 3 0 0 1 6 253 10 0.0 3 0 0 1 9 254 7 0.0 3 0 0 0 7 255 8 0.0 3 0 0 0 8 256 1 0.0 3 0 0 0 1 257 4 0.0 3 0 0 1 3 258 1 0.0 3 0 0 0 1 260 3 0.0 3 0 0 0 3 261 9 0.0 3 0 0 1 8 262 7 0.0 3 0 0 0 7 263 15 0.0 3 0 0 0 15 265 4 0.0 3 0 0 0 4 266 2 0.0 3 0 0 0 2 267 4 0.0 3 0 0 0 4 268 3 0.0 3 0 0 0 3 269 1 0.0 3 0 0 0 1 270 6 0.0 3 0 0 0 6 271 62 0.0 3 0 0 1 61 272 11 0.0 3 0 0 0 11 273 26 0.0 3 0 0 0 26 274 19 0.0 3 0 0 2 17 275 15 0.0 3 0 1 3 11 276 11 0.0 3 0 0 2 9 277 4 0.0 3 0 0 0 4 278 3 0.0 3 0 0 1 2 279 7 0.0 3 0 0 0 7 280 76 0.0 3 0 0 2 74 281 36 0.0 3 0 0 4 32 282 8 0.0 3 0 0 0 8 283 5 0.0 3 0 0 1 4 284 4 0.0 3 0 0 0 4 285 1 0.0 3 0 0 0 1 286 1 0.0 3 0 0 0 1 287 2 0.0 3 0 0 0 2 288 9 0.0 3 0 0 0 9 289 2 0.0 3 0 0 0 2 291 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.73 s (33 us/read; 1.82 M reads/minute). === Summary === Total reads processed: 22,087 Reads with adapters: 125 (0.6%) Reads written (passing filters): 22,087 (100.0%) Total basepairs processed: 5,627,526 bp Total written (filtered): 5,627,132 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 125 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 48.8% C: 34.4% G: 14.4% T: 2.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 113 345.1 0 113 4 6 86.3 0 6 5 5 21.6 0 5 6 1 5.4 0 1