This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACCTAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 68.89 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 209,699 (8.0%) Reads that were too short: 15,690 (0.6%) Reads written (passing filters): 194,009 (7.4%) Total basepairs processed: 791,719,999 bp Total written (filtered): 54,247,535 bp (6.9%) === Adapter 1 === Sequence: ACCTAT; Type: regular 5'; Length: 6; Trimmed: 209699 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 723 642.2 0 723 7 18300 642.2 0 18300 8 704 642.2 0 704 9 19738 642.2 0 19738 10 610 642.2 0 610 11 1297 642.2 0 1297 12 14481 642.2 0 14481 13 105716 642.2 0 105716 14 15781 642.2 0 15781 15 64 642.2 0 64 16 46 642.2 0 46 17 47 642.2 0 47 18 34 642.2 0 34 19 30 642.2 0 30 20 40 642.2 0 40 21 27 642.2 0 27 22 43 642.2 0 43 23 42 642.2 0 42 24 50 642.2 0 50 25 40 642.2 0 40 26 38 642.2 0 38 27 41 642.2 0 41 28 47 642.2 0 47 29 54 642.2 0 54 30 47 642.2 0 47 31 46 642.2 0 46 32 43 642.2 0 43 33 35 642.2 0 35 34 28 642.2 0 28 35 36 642.2 0 36 36 40 642.2 0 40 37 37 642.2 0 37 38 116 642.2 0 116 39 34 642.2 0 34 40 84 642.2 0 84 41 57 642.2 0 57 42 46 642.2 0 46 43 120 642.2 0 120 44 103 642.2 0 103 45 81 642.2 0 81 46 48 642.2 0 48 47 31 642.2 0 31 48 49 642.2 0 49 49 54 642.2 0 54 50 67 642.2 0 67 51 43 642.2 0 43 52 33 642.2 0 33 53 58 642.2 0 58 54 45 642.2 0 45 55 49 642.2 0 49 56 38 642.2 0 38 57 53 642.2 0 53 58 51 642.2 0 51 59 50 642.2 0 50 60 42 642.2 0 42 61 51 642.2 0 51 62 43 642.2 0 43 63 45 642.2 0 45 64 51 642.2 0 51 65 51 642.2 0 51 66 54 642.2 0 54 67 60 642.2 0 60 68 40 642.2 0 40 69 39 642.2 0 39 70 65 642.2 0 65 71 38 642.2 0 38 72 31 642.2 0 31 73 53 642.2 0 53 74 48 642.2 0 48 75 38 642.2 0 38 76 49 642.2 0 49 77 66 642.2 0 66 78 70 642.2 0 70 79 65 642.2 0 65 80 75 642.2 0 75 81 49 642.2 0 49 82 55 642.2 0 55 83 60 642.2 0 60 84 46 642.2 0 46 85 48 642.2 0 48 86 76 642.2 0 76 87 66 642.2 0 66 88 66 642.2 0 66 89 79 642.2 0 79 90 305 642.2 0 305 91 84 642.2 0 84 92 115 642.2 0 115 93 53 642.2 0 53 94 76 642.2 0 76 95 52 642.2 0 52 96 62 642.2 0 62 97 66 642.2 0 66 98 81 642.2 0 81 99 45 642.2 0 45 100 54 642.2 0 54 101 46 642.2 0 46 102 87 642.2 0 87 103 78 642.2 0 78 104 183 642.2 0 183 105 97 642.2 0 97 106 222 642.2 0 222 107 78 642.2 0 78 108 87 642.2 0 87 109 67 642.2 0 67 110 89 642.2 0 89 111 90 642.2 0 90 112 90 642.2 0 90 113 75 642.2 0 75 114 71 642.2 0 71 115 99 642.2 0 99 116 93 642.2 0 93 117 64 642.2 0 64 118 64 642.2 0 64 119 58 642.2 0 58 120 61 642.2 0 61 121 71 642.2 0 71 122 85 642.2 0 85 123 53 642.2 0 53 124 81 642.2 0 81 125 63 642.2 0 63 126 244 642.2 0 244 127 692 642.2 0 692 128 322 642.2 0 322 129 136 642.2 0 136 130 68 642.2 0 68 131 56 642.2 0 56 132 62 642.2 0 62 133 64 642.2 0 64 134 153 642.2 0 153 135 118 642.2 0 118 136 62 642.2 0 62 137 70 642.2 0 70 138 125 642.2 0 125 139 232 642.2 0 232 140 406 642.2 0 406 141 319 642.2 0 319 142 163 642.2 0 163 143 57 642.2 0 57 144 73 642.2 0 73 145 58 642.2 0 58 146 83 642.2 0 83 147 75 642.2 0 75 148 62 642.2 0 62 149 52 642.2 0 52 150 71 642.2 0 71 151 191 642.2 0 191 152 148 642.2 0 148 153 275 642.2 0 275 154 170 642.2 0 170 155 79 642.2 0 79 156 77 642.2 0 77 157 72 642.2 0 72 158 123 642.2 0 123 159 254 642.2 0 254 160 908 642.2 0 908 161 368 642.2 0 368 162 139 642.2 0 139 163 180 642.2 0 180 164 81 642.2 0 81 165 70 642.2 0 70 166 46 642.2 0 46 167 67 642.2 0 67 168 86 642.2 0 86 169 169 642.2 0 169 170 61 642.2 0 61 171 83 642.2 0 83 172 47 642.2 0 47 173 52 642.2 0 52 174 83 642.2 0 83 175 68 642.2 0 68 176 72 642.2 0 72 177 53 642.2 0 53 178 68 642.2 0 68 179 74 642.2 0 74 180 64 642.2 0 64 181 89 642.2 0 89 182 131 642.2 0 131 183 58 642.2 0 58 184 75 642.2 0 75 185 64 642.2 0 64 186 169 642.2 0 169 187 73 642.2 0 73 188 91 642.2 0 91 189 82 642.2 0 82 190 80 642.2 0 80 191 49 642.2 0 49 192 59 642.2 0 59 193 70 642.2 0 70 194 63 642.2 0 63 195 44 642.2 0 44 196 54 642.2 0 54 197 63 642.2 0 63 198 63 642.2 0 63 199 75 642.2 0 75 200 59 642.2 0 59 201 68 642.2 0 68 202 87 642.2 0 87 203 81 642.2 0 81 204 63 642.2 0 63 205 60 642.2 0 60 206 64 642.2 0 64 207 61 642.2 0 61 208 77 642.2 0 77 209 92 642.2 0 92 210 53 642.2 0 53 211 60 642.2 0 60 212 45 642.2 0 45 213 67 642.2 0 67 214 150 642.2 0 150 215 130 642.2 0 130 216 89 642.2 0 89 217 77 642.2 0 77 218 57 642.2 0 57 219 61 642.2 0 61 220 137 642.2 0 137 221 213 642.2 0 213 222 170 642.2 0 170 223 123 642.2 0 123 224 242 642.2 0 242 225 96 642.2 0 96 226 326 642.2 0 326 227 78 642.2 0 78 228 59 642.2 0 59 229 60 642.2 0 60 230 83 642.2 0 83 231 88 642.2 0 88 232 146 642.2 0 146 233 258 642.2 0 258 234 726 642.2 0 726 235 185 642.2 0 185 236 176 642.2 0 176 237 87 642.2 0 87 238 55 642.2 0 55 239 55 642.2 0 55 240 64 642.2 0 64 241 91 642.2 0 91 242 99 642.2 0 99 243 69 642.2 0 69 244 54 642.2 0 54 245 62 642.2 0 62 246 52 642.2 0 52 247 52 642.2 0 52 248 74 642.2 0 74 249 47 642.2 0 47 250 81 642.2 0 81 251 72 642.2 0 72 252 78 642.2 0 78 253 102 642.2 0 102 254 60 642.2 0 60 255 57 642.2 0 57 256 56 642.2 0 56 257 88 642.2 0 88 258 62 642.2 0 62 259 41 642.2 0 41 260 77 642.2 0 77 261 91 642.2 0 91 262 86 642.2 0 86 263 81 642.2 0 81 264 69 642.2 0 69 265 69 642.2 0 69 266 66 642.2 0 66 267 110 642.2 0 110 268 129 642.2 0 129 269 187 642.2 0 187 270 74 642.2 0 74 271 68 642.2 0 68 272 79 642.2 0 79 273 69 642.2 0 69 274 87 642.2 0 87 275 95 642.2 0 95 276 107 642.2 0 107 277 491 642.2 0 491 278 294 642.2 0 294 279 295 642.2 0 295 280 319 642.2 0 319 281 1274 642.2 0 1274 282 1712 642.2 0 1712 283 1024 642.2 0 1024 284 323 642.2 0 323 285 65 642.2 0 65 286 101 642.2 0 101 287 123 642.2 0 123 288 286 642.2 0 286 289 148 642.2 0 148 290 624 642.2 0 624 291 111 642.2 0 111 292 107 642.2 0 107 293 71 642.2 0 71 294 98 642.2 0 98 295 132 642.2 0 132 296 113 642.2 0 113 297 84 642.2 0 84 298 48 642.2 0 48 299 60 642.2 0 60 300 62 642.2 0 62 301 83 642.2 0 83 Finished in 9.89 s (51 us/read; 1.18 M reads/minute). === Summary === Total reads processed: 194,009 Reads with adapters: 22,459 (11.6%) Reads that were too short: 76 (0.0%) Reads written (passing filters): 22,383 (11.5%) Total basepairs processed: 54,247,535 bp Total written (filtered): 5,823,729 bp (10.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 22459 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 3 0.0 3 0 0 3 19 56 0.0 3 0 53 2 1 20 19700 0.0 4 19388 303 7 2 21 52 0.0 4 8 42 2 22 15 0.0 4 0 0 2 0 13 23 30 0.0 4 19 0 0 10 1 29 1 0.0 4 0 0 0 0 1 30 21 0.0 4 0 0 0 0 21 39 1 0.0 4 0 0 0 0 1 69 8 0.0 4 1 0 0 0 7 78 4 0.0 4 0 0 0 0 4 80 1 0.0 4 0 0 0 0 1 86 1 0.0 4 0 0 0 0 1 87 1 0.0 4 0 0 0 0 1 96 2 0.0 4 0 1 0 0 1 97 1 0.0 4 1 106 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 120 4 0.0 4 0 1 0 0 3 121 9 0.0 4 0 0 0 0 9 122 2134 0.0 4 0 0 0 0 2134 123 13 0.0 4 0 0 0 0 13 125 4 0.0 4 0 0 0 0 4 126 2 0.0 4 0 0 0 0 2 127 292 0.0 4 0 0 0 0 292 128 2 0.0 4 0 0 0 0 2 130 1 0.0 4 0 0 0 0 1 133 2 0.0 4 2 137 1 0.0 4 0 0 0 0 1 148 7 0.0 4 0 0 0 0 7 149 2 0.0 4 2 151 3 0.0 4 1 0 0 0 2 152 1 0.0 4 1 154 2 0.0 4 2 155 2 0.0 4 2 161 1 0.0 4 0 0 0 1 163 1 0.0 4 0 0 0 0 1 165 1 0.0 4 0 0 0 0 1 167 2 0.0 4 0 0 0 0 2 175 1 0.0 4 1 183 2 0.0 4 1 0 0 0 1 197 1 0.0 4 1 198 1 0.0 4 1 199 1 0.0 4 1 201 1 0.0 4 1 202 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 204 1 0.0 4 0 0 0 0 1 209 2 0.0 4 0 0 0 0 2 218 1 0.0 4 0 0 0 0 1 220 2 0.0 4 0 1 0 1 222 2 0.0 4 0 2 223 5 0.0 4 0 0 0 0 5 224 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 3 0.0 4 0 0 0 0 3 229 4 0.0 4 0 0 0 0 4 230 1 0.0 4 0 0 0 0 1 231 1 0.0 4 0 0 0 0 1 232 3 0.0 4 0 0 0 0 3 234 1 0.0 4 0 1 250 3 0.0 4 0 0 0 0 3 252 1 0.0 4 0 0 1 253 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 259 2 0.0 4 1 0 0 0 1 262 1 0.0 4 0 0 1 265 1 0.0 4 0 0 0 0 1 267 2 0.0 4 0 0 0 1 1 269 1 0.0 4 0 0 0 0 1 270 4 0.0 4 1 0 0 1 2 272 1 0.0 4 0 0 0 0 1 276 1 0.0 4 0 0 0 0 1 277 2 0.0 4 0 0 0 0 2 278 5 0.0 4 2 1 0 1 1 279 4 0.0 4 0 2 1 0 1 281 2 0.0 4 1 0 0 1 282 1 0.0 4 0 0 0 0 1 284 1 0.0 4 0 0 0 0 1 288 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.80 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 22,383 Reads with adapters: 4,609 (20.6%) Reads written (passing filters): 22,383 (100.0%) Total basepairs processed: 5,823,729 bp Total written (filtered): 5,771,344 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 4609 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.8% C: 3.4% G: 94.4% T: 1.4% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 6 349.7 0 6 4 24 87.4 0 24 6 1 5.5 0 1 7 2 1.4 0 2 8 1 0.3 0 1 9 3066 0.1 0 99 2967 10 1324 0.0 1 55 1269 11 114 0.0 1 0 114 12 2 0.0 1 0 2 13 3 0.0 1 0 3 59 1 0.0 1 1 68 1 0.0 1 1 79 1 0.0 1 0 1 80 11 0.0 1 11 89 1 0.0 1 1 107 1 0.0 1 1 114 21 0.0 1 21 126 6 0.0 1 5 1 152 1 0.0 1 0 1 158 1 0.0 1 1 207 1 0.0 1 1 208 1 0.0 1 1 223 3 0.0 1 3 266 6 0.0 1 5 1 267 10 0.0 1 10 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.