This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TATACC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 67.89 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 76,618 (2.9%) Reads that were too short: 9,795 (0.4%) Reads written (passing filters): 66,823 (2.5%) Total basepairs processed: 791,719,999 bp Total written (filtered): 18,174,662 bp (2.3%) === Adapter 1 === Sequence: TATACC; Type: regular 5'; Length: 6; Trimmed: 76618 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1019 642.2 0 1019 7 30414 642.2 0 30414 8 261 642.2 0 261 9 21975 642.2 0 21975 10 66 642.2 0 66 11 45 642.2 0 45 12 33 642.2 0 33 13 44 642.2 0 44 14 34 642.2 0 34 15 37 642.2 0 37 16 46 642.2 0 46 17 38 642.2 0 38 18 42 642.2 0 42 19 46 642.2 0 46 20 56 642.2 0 56 21 64 642.2 0 64 22 51 642.2 0 51 23 71 642.2 0 71 24 61 642.2 0 61 25 34 642.2 0 34 26 51 642.2 0 51 27 40 642.2 0 40 28 37 642.2 0 37 29 62 642.2 0 62 30 32 642.2 0 32 31 35 642.2 0 35 32 39 642.2 0 39 33 40 642.2 0 40 34 33 642.2 0 33 35 43 642.2 0 43 36 37 642.2 0 37 37 25 642.2 0 25 38 32 642.2 0 32 39 45 642.2 0 45 40 41 642.2 0 41 41 37 642.2 0 37 42 50 642.2 0 50 43 48 642.2 0 48 44 44 642.2 0 44 45 38 642.2 0 38 46 56 642.2 0 56 47 43 642.2 0 43 48 41 642.2 0 41 49 51 642.2 0 51 50 84 642.2 0 84 51 44 642.2 0 44 52 53 642.2 0 53 53 55 642.2 0 55 54 46 642.2 0 46 55 70 642.2 0 70 56 48 642.2 0 48 57 35 642.2 0 35 58 69 642.2 0 69 59 39 642.2 0 39 60 42 642.2 0 42 61 39 642.2 0 39 62 32 642.2 0 32 63 49 642.2 0 49 64 66 642.2 0 66 65 54 642.2 0 54 66 52 642.2 0 52 67 61 642.2 0 61 68 47 642.2 0 47 69 48 642.2 0 48 70 70 642.2 0 70 71 265 642.2 0 265 72 63 642.2 0 63 73 76 642.2 0 76 74 80 642.2 0 80 75 93 642.2 0 93 76 52 642.2 0 52 77 45 642.2 0 45 78 38 642.2 0 38 79 37 642.2 0 37 80 59 642.2 0 59 81 59 642.2 0 59 82 48 642.2 0 48 83 37 642.2 0 37 84 44 642.2 0 44 85 44 642.2 0 44 86 63 642.2 0 63 87 55 642.2 0 55 88 34 642.2 0 34 89 58 642.2 0 58 90 59 642.2 0 59 91 43 642.2 0 43 92 45 642.2 0 45 93 42 642.2 0 42 94 40 642.2 0 40 95 52 642.2 0 52 96 51 642.2 0 51 97 30 642.2 0 30 98 35 642.2 0 35 99 52 642.2 0 52 100 56 642.2 0 56 101 246 642.2 0 246 102 157 642.2 0 157 103 229 642.2 0 229 104 63 642.2 0 63 105 53 642.2 0 53 106 49 642.2 0 49 107 52 642.2 0 52 108 49 642.2 0 49 109 49 642.2 0 49 110 46 642.2 0 46 111 155 642.2 0 155 112 137 642.2 0 137 113 126 642.2 0 126 114 61 642.2 0 61 115 62 642.2 0 62 116 56 642.2 0 56 117 73 642.2 0 73 118 54 642.2 0 54 119 50 642.2 0 50 120 41 642.2 0 41 121 44 642.2 0 44 122 47 642.2 0 47 123 68 642.2 0 68 124 87 642.2 0 87 125 66 642.2 0 66 126 64 642.2 0 64 127 49 642.2 0 49 128 38 642.2 0 38 129 37 642.2 0 37 130 66 642.2 0 66 131 78 642.2 0 78 132 288 642.2 0 288 133 154 642.2 0 154 134 148 642.2 0 148 135 39 642.2 0 39 136 58 642.2 0 58 137 103 642.2 0 103 138 154 642.2 0 154 139 113 642.2 0 113 140 56 642.2 0 56 141 90 642.2 0 90 142 90 642.2 0 90 143 80 642.2 0 80 144 208 642.2 0 208 145 232 642.2 0 232 146 195 642.2 0 195 147 43 642.2 0 43 148 113 642.2 0 113 149 111 642.2 0 111 150 131 642.2 0 131 151 84 642.2 0 84 152 49 642.2 0 49 153 55 642.2 0 55 154 90 642.2 0 90 155 82 642.2 0 82 156 89 642.2 0 89 157 55 642.2 0 55 158 82 642.2 0 82 159 63 642.2 0 63 160 43 642.2 0 43 161 49 642.2 0 49 162 46 642.2 0 46 163 76 642.2 0 76 164 52 642.2 0 52 165 55 642.2 0 55 166 83 642.2 0 83 167 52 642.2 0 52 168 78 642.2 0 78 169 110 642.2 0 110 170 229 642.2 0 229 171 157 642.2 0 157 172 171 642.2 0 171 173 37 642.2 0 37 174 41 642.2 0 41 175 45 642.2 0 45 176 45 642.2 0 45 177 78 642.2 0 78 178 107 642.2 0 107 179 112 642.2 0 112 180 174 642.2 0 174 181 58 642.2 0 58 182 54 642.2 0 54 183 57 642.2 0 57 184 41 642.2 0 41 185 53 642.2 0 53 186 61 642.2 0 61 187 55 642.2 0 55 188 63 642.2 0 63 189 61 642.2 0 61 190 73 642.2 0 73 191 47 642.2 0 47 192 45 642.2 0 45 193 56 642.2 0 56 194 60 642.2 0 60 195 42 642.2 0 42 196 53 642.2 0 53 197 66 642.2 0 66 198 68 642.2 0 68 199 70 642.2 0 70 200 47 642.2 0 47 201 51 642.2 0 51 202 75 642.2 0 75 203 59 642.2 0 59 204 73 642.2 0 73 205 76 642.2 0 76 206 46 642.2 0 46 207 58 642.2 0 58 208 45 642.2 0 45 209 96 642.2 0 96 210 169 642.2 0 169 211 69 642.2 0 69 212 72 642.2 0 72 213 63 642.2 0 63 214 62 642.2 0 62 215 51 642.2 0 51 216 61 642.2 0 61 217 65 642.2 0 65 218 62 642.2 0 62 219 65 642.2 0 65 220 43 642.2 0 43 221 40 642.2 0 40 222 45 642.2 0 45 223 85 642.2 0 85 224 86 642.2 0 86 225 96 642.2 0 96 226 94 642.2 0 94 227 111 642.2 0 111 228 77 642.2 0 77 229 73 642.2 0 73 230 48 642.2 0 48 231 42 642.2 0 42 232 64 642.2 0 64 233 92 642.2 0 92 234 72 642.2 0 72 235 53 642.2 0 53 236 76 642.2 0 76 237 71 642.2 0 71 238 74 642.2 0 74 239 54 642.2 0 54 240 56 642.2 0 56 241 70 642.2 0 70 242 59 642.2 0 59 243 77 642.2 0 77 244 104 642.2 0 104 245 120 642.2 0 120 246 128 642.2 0 128 247 142 642.2 0 142 248 129 642.2 0 129 249 77 642.2 0 77 250 72 642.2 0 72 251 75 642.2 0 75 252 52 642.2 0 52 253 73 642.2 0 73 254 66 642.2 0 66 255 68 642.2 0 68 256 99 642.2 0 99 257 119 642.2 0 119 258 125 642.2 0 125 259 108 642.2 0 108 260 122 642.2 0 122 261 105 642.2 0 105 262 140 642.2 0 140 263 98 642.2 0 98 264 139 642.2 0 139 265 112 642.2 0 112 266 172 642.2 0 172 267 65 642.2 0 65 268 79 642.2 0 79 269 77 642.2 0 77 270 75 642.2 0 75 271 111 642.2 0 111 272 126 642.2 0 126 273 134 642.2 0 134 274 98 642.2 0 98 275 70 642.2 0 70 276 69 642.2 0 69 277 78 642.2 0 78 278 64 642.2 0 64 279 80 642.2 0 80 280 84 642.2 0 84 281 90 642.2 0 90 282 96 642.2 0 96 283 118 642.2 0 118 284 420 642.2 0 420 285 467 642.2 0 467 286 493 642.2 0 493 287 336 642.2 0 336 288 114 642.2 0 114 289 77 642.2 0 77 290 59 642.2 0 59 291 66 642.2 0 66 292 96 642.2 0 96 293 125 642.2 0 125 294 79 642.2 0 79 295 65 642.2 0 65 296 76 642.2 0 76 297 68 642.2 0 68 298 84 642.2 0 84 299 76 642.2 0 76 300 93 642.2 0 93 301 117 642.2 0 117 Finished in 3.35 s (50 us/read; 1.20 M reads/minute). === Summary === Total reads processed: 66,823 Reads with adapters: 31,270 (46.8%) Reads that were too short: 11 (0.0%) Reads written (passing filters): 31,259 (46.8%) Total basepairs processed: 18,174,662 bp Total written (filtered): 8,539,324 bp (47.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 31270 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 3 0.0 3 0 0 3 19 106 0.0 3 0 104 2 20 30392 0.0 4 29907 474 10 0 1 21 56 0.0 4 6 50 22 2 0.0 4 0 1 1 23 469 0.0 4 450 7 0 11 1 24 2 0.0 4 0 1 0 0 1 34 1 0.0 4 1 43 1 0.0 4 0 0 0 0 1 48 1 0.0 4 0 0 0 0 1 96 1 0.0 4 0 0 0 0 1 109 1 0.0 4 1 111 1 0.0 4 1 112 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 222 0.0 4 0 0 0 0 222 130 1 0.0 4 0 0 0 0 1 132 1 0.0 4 0 0 0 0 1 147 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 228 2 0.0 4 0 0 0 0 2 229 3 0.0 4 0 0 0 1 2 234 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.07 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 31,259 Reads with adapters: 1,595 (5.1%) Reads written (passing filters): 31,259 (100.0%) Total basepairs processed: 8,539,324 bp Total written (filtered): 8,511,533 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1595 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 64.5% C: 3.0% G: 31.5% T: 0.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 81 488.4 0 81 4 6 122.1 0 6 6 2 7.6 0 2 8 4 0.5 0 4 9 93 0.1 0 1 92 10 34 0.0 1 1 33 11 313 0.0 1 4 309 12 42 0.0 1 0 42 13 1 0.0 1 0 1 19 5 0.0 1 4 1 20 918 0.0 1 732 186 21 45 0.0 1 36 9 22 1 0.0 1 0 1 24 36 0.0 1 28 8 25 3 0.0 1 2 1 28 1 0.0 1 0 1 185 1 0.0 1 1 186 4 0.0 1 4 187 2 0.0 1 2 191 2 0.0 1 0 2 269 1 0.0 1 1