This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCTTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 69.69 s (26 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 449,114 (17.1%) Reads that were too short: 140,673 (5.3%) Reads written (passing filters): 308,441 (11.7%) Total basepairs processed: 791,719,999 bp Total written (filtered): 88,082,888 bp (11.1%) === Adapter 1 === Sequence: GTCTTA; Type: regular 5'; Length: 6; Trimmed: 449114 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1905 642.2 0 1905 7 6326 642.2 0 6326 8 172889 642.2 0 172889 9 105077 642.2 0 105077 10 132 642.2 0 132 11 34 642.2 0 34 12 36 642.2 0 36 13 42 642.2 0 42 14 38 642.2 0 38 15 39 642.2 0 39 16 28 642.2 0 28 17 22 642.2 0 22 18 16 642.2 0 16 19 30 642.2 0 30 20 43 642.2 0 43 21 28 642.2 0 28 22 40 642.2 0 40 23 27 642.2 0 27 24 32 642.2 0 32 25 36 642.2 0 36 26 52 642.2 0 52 27 35 642.2 0 35 28 38 642.2 0 38 29 35 642.2 0 35 30 31 642.2 0 31 31 29 642.2 0 29 32 26 642.2 0 26 33 43 642.2 0 43 34 42 642.2 0 42 35 34 642.2 0 34 36 73 642.2 0 73 37 25 642.2 0 25 38 95 642.2 0 95 39 51 642.2 0 51 40 45 642.2 0 45 41 79 642.2 0 79 42 69 642.2 0 69 43 1157 642.2 0 1157 44 57 642.2 0 57 45 34 642.2 0 34 46 31 642.2 0 31 47 28 642.2 0 28 48 54 642.2 0 54 49 50 642.2 0 50 50 54 642.2 0 54 51 56 642.2 0 56 52 33 642.2 0 33 53 31 642.2 0 31 54 38 642.2 0 38 55 51 642.2 0 51 56 128 642.2 0 128 57 133 642.2 0 133 58 101 642.2 0 101 59 52 642.2 0 52 60 48 642.2 0 48 61 107 642.2 0 107 62 65 642.2 0 65 63 46 642.2 0 46 64 71 642.2 0 71 65 47 642.2 0 47 66 29 642.2 0 29 67 64 642.2 0 64 68 122 642.2 0 122 69 384 642.2 0 384 70 405 642.2 0 405 71 344 642.2 0 344 72 66 642.2 0 66 73 71 642.2 0 71 74 74 642.2 0 74 75 84 642.2 0 84 76 107 642.2 0 107 77 59 642.2 0 59 78 74 642.2 0 74 79 71 642.2 0 71 80 57 642.2 0 57 81 56 642.2 0 56 82 60 642.2 0 60 83 65 642.2 0 65 84 49 642.2 0 49 85 48 642.2 0 48 86 91 642.2 0 91 87 99 642.2 0 99 88 140 642.2 0 140 89 2630 642.2 0 2630 90 842 642.2 0 842 91 2774 642.2 0 2774 92 58 642.2 0 58 93 45 642.2 0 45 94 57 642.2 0 57 95 54 642.2 0 54 96 56 642.2 0 56 97 140 642.2 0 140 98 80 642.2 0 80 99 105 642.2 0 105 100 89 642.2 0 89 101 66 642.2 0 66 102 80 642.2 0 80 103 36 642.2 0 36 104 62 642.2 0 62 105 44 642.2 0 44 106 51 642.2 0 51 107 41 642.2 0 41 108 81 642.2 0 81 109 50 642.2 0 50 110 47 642.2 0 47 111 73 642.2 0 73 112 62 642.2 0 62 113 48 642.2 0 48 114 20 642.2 0 20 115 25 642.2 0 25 116 46 642.2 0 46 117 45 642.2 0 45 118 47 642.2 0 47 119 106 642.2 0 106 120 38 642.2 0 38 121 51 642.2 0 51 122 36 642.2 0 36 123 37 642.2 0 37 124 29 642.2 0 29 125 26 642.2 0 26 126 37 642.2 0 37 127 47 642.2 0 47 128 23 642.2 0 23 129 49 642.2 0 49 130 42 642.2 0 42 131 31 642.2 0 31 132 74 642.2 0 74 133 44 642.2 0 44 134 38 642.2 0 38 135 48 642.2 0 48 136 70 642.2 0 70 137 294 642.2 0 294 138 91 642.2 0 91 139 63 642.2 0 63 140 37 642.2 0 37 141 51 642.2 0 51 142 40 642.2 0 40 143 52 642.2 0 52 144 44 642.2 0 44 145 47 642.2 0 47 146 37 642.2 0 37 147 55 642.2 0 55 148 228 642.2 0 228 149 128 642.2 0 128 150 455 642.2 0 455 151 41 642.2 0 41 152 37 642.2 0 37 153 69 642.2 0 69 154 66 642.2 0 66 155 71 642.2 0 71 156 220 642.2 0 220 157 127 642.2 0 127 158 144 642.2 0 144 159 81 642.2 0 81 160 37 642.2 0 37 161 58 642.2 0 58 162 44 642.2 0 44 163 44 642.2 0 44 164 48 642.2 0 48 165 51 642.2 0 51 166 29 642.2 0 29 167 36 642.2 0 36 168 184 642.2 0 184 169 111 642.2 0 111 170 104 642.2 0 104 171 26 642.2 0 26 172 29 642.2 0 29 173 40 642.2 0 40 174 62 642.2 0 62 175 101 642.2 0 101 176 729 642.2 0 729 177 1352 642.2 0 1352 178 352 642.2 0 352 179 87 642.2 0 87 180 47 642.2 0 47 181 23 642.2 0 23 182 75 642.2 0 75 183 69 642.2 0 69 184 83 642.2 0 83 185 38 642.2 0 38 186 44 642.2 0 44 187 31 642.2 0 31 188 36 642.2 0 36 189 62 642.2 0 62 190 30 642.2 0 30 191 33 642.2 0 33 192 41 642.2 0 41 193 32 642.2 0 32 194 47 642.2 0 47 195 58 642.2 0 58 196 43 642.2 0 43 197 45 642.2 0 45 198 59 642.2 0 59 199 36 642.2 0 36 200 43 642.2 0 43 201 71 642.2 0 71 202 157 642.2 0 157 203 128 642.2 0 128 204 90 642.2 0 90 205 94 642.2 0 94 206 60 642.2 0 60 207 176 642.2 0 176 208 190 642.2 0 190 209 188 642.2 0 188 210 154 642.2 0 154 211 118 642.2 0 118 212 123 642.2 0 123 213 54 642.2 0 54 214 52 642.2 0 52 215 76 642.2 0 76 216 95 642.2 0 95 217 94 642.2 0 94 218 106 642.2 0 106 219 276 642.2 0 276 220 1880 642.2 0 1880 221 1077 642.2 0 1077 222 669 642.2 0 669 223 488 642.2 0 488 224 52 642.2 0 52 225 83 642.2 0 83 226 45 642.2 0 45 227 53 642.2 0 53 228 48 642.2 0 48 229 28 642.2 0 28 230 108 642.2 0 108 231 106 642.2 0 106 232 164 642.2 0 164 233 102 642.2 0 102 234 57 642.2 0 57 235 67 642.2 0 67 236 68 642.2 0 68 237 55 642.2 0 55 238 61 642.2 0 61 239 118 642.2 0 118 240 126 642.2 0 126 241 174 642.2 0 174 242 184 642.2 0 184 243 178 642.2 0 178 244 257 642.2 0 257 245 193 642.2 0 193 246 125 642.2 0 125 247 252 642.2 0 252 248 72 642.2 0 72 249 89 642.2 0 89 250 78 642.2 0 78 251 118 642.2 0 118 252 80 642.2 0 80 253 93 642.2 0 93 254 113 642.2 0 113 255 145 642.2 0 145 256 168 642.2 0 168 257 218 642.2 0 218 258 71 642.2 0 71 259 203 642.2 0 203 260 108 642.2 0 108 261 167 642.2 0 167 262 438 642.2 0 438 263 395 642.2 0 395 264 487 642.2 0 487 265 192 642.2 0 192 266 209 642.2 0 209 267 126 642.2 0 126 268 436 642.2 0 436 269 824 642.2 0 824 270 1076 642.2 0 1076 271 1433 642.2 0 1433 272 213 642.2 0 213 273 97 642.2 0 97 274 583 642.2 0 583 275 322 642.2 0 322 276 815 642.2 0 815 277 266 642.2 0 266 278 563 642.2 0 563 279 721 642.2 0 721 280 1516 642.2 0 1516 281 4041 642.2 0 4041 282 17350 642.2 0 17350 283 37934 642.2 0 37934 284 37046 642.2 0 37046 285 14035 642.2 0 14035 286 6527 642.2 0 6527 287 92 642.2 0 92 288 343 642.2 0 343 289 742 642.2 0 742 290 334 642.2 0 334 291 177 642.2 0 177 292 69 642.2 0 69 293 42 642.2 0 42 294 45 642.2 0 45 295 47 642.2 0 47 296 84 642.2 0 84 297 83 642.2 0 83 298 73 642.2 0 73 299 56 642.2 0 56 300 47 642.2 0 47 301 22 642.2 0 22 Finished in 13.34 s (43 us/read; 1.39 M reads/minute). === Summary === Total reads processed: 308,441 Reads with adapters: 177,280 (57.5%) Reads that were too short: 215 (0.1%) Reads written (passing filters): 177,065 (57.4%) Total basepairs processed: 88,082,888 bp Total written (filtered): 48,837,319 bp (55.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 177280 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 43 0.0 2 0 0 0 43 15 104 0.0 3 0 0 58 46 16 1244 0.0 3 0 907 239 98 17 173706 0.0 3 162707 8873 1726 400 18 619 0.0 3 37 381 92 109 19 265 0.0 3 0 1 161 103 20 837 0.0 3 0 0 0 837 21 2 0.0 3 0 0 0 2 24 1 0.0 3 0 0 0 1 25 1 0.0 3 1 27 1 0.0 3 1 28 1 0.0 3 1 31 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 33 3 0.0 3 2 0 0 1 36 1 0.0 3 1 37 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 43 24 0.0 3 0 0 1 23 45 1 0.0 3 0 0 0 1 46 3 0.0 3 0 0 0 3 48 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 1 65 2 0.0 3 0 0 0 2 69 1 0.0 3 0 0 0 1 71 3 0.0 3 0 0 0 3 75 1 0.0 3 0 0 0 1 77 4 0.0 3 0 0 0 4 78 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 80 3 0.0 3 1 0 0 2 83 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 94 2 0.0 3 0 0 1 1 98 1 0.0 3 0 0 0 1 100 3 0.0 3 0 0 0 3 101 97 0.0 3 0 0 2 95 102 36 0.0 3 0 0 0 36 103 6 0.0 3 0 0 0 6 105 9 0.0 3 0 0 0 9 106 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 128 4 0.0 3 0 0 0 4 131 1 0.0 3 0 0 0 1 133 3 0.0 3 0 0 0 3 135 2 0.0 3 0 0 0 2 136 1 0.0 3 0 0 0 1 137 2 0.0 3 0 0 0 2 138 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 144 2 0.0 3 0 0 0 2 146 1 0.0 3 0 0 0 1 153 2 0.0 3 0 0 0 2 154 1 0.0 3 0 0 0 1 156 1 0.0 3 1 157 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 177 1 0.0 3 1 178 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 180 3 0.0 3 0 0 0 3 183 2 0.0 3 0 0 0 2 184 1 0.0 3 0 0 0 1 187 3 0.0 3 0 0 1 2 188 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 192 14 0.0 3 0 0 1 13 193 2 0.0 3 0 0 0 2 194 8 0.0 3 0 0 0 8 202 1 0.0 3 0 0 0 1 203 4 0.0 3 0 0 0 4 204 2 0.0 3 0 0 0 2 205 2 0.0 3 0 0 0 2 206 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 211 2 0.0 3 0 0 0 2 215 1 0.0 3 0 0 0 1 216 2 0.0 3 0 0 0 2 217 50 0.0 3 0 0 8 42 218 3 0.0 3 0 0 0 3 219 1 0.0 3 0 0 0 1 221 2 0.0 3 0 0 0 2 222 1 0.0 3 0 0 0 1 233 1 0.0 3 0 0 0 1 234 1 0.0 3 0 0 1 238 6 0.0 3 0 0 0 6 239 1 0.0 3 0 0 0 1 243 1 0.0 3 0 0 0 1 248 2 0.0 3 0 0 1 1 257 1 0.0 3 0 0 0 1 259 1 0.0 3 0 0 0 1 260 1 0.0 3 0 0 0 1 261 1 0.0 3 0 0 0 1 262 1 0.0 3 0 0 0 1 264 1 0.0 3 0 0 0 1 266 1 0.0 3 0 0 0 1 270 4 0.0 3 0 0 0 4 278 2 0.0 3 0 0 0 2 279 13 0.0 3 0 0 0 13 280 5 0.0 3 0 0 1 4 281 28 0.0 3 0 1 1 26 282 15 0.0 3 0 0 0 15 283 1 0.0 3 0 0 0 1 288 4 0.0 3 0 0 0 4 289 2 0.0 3 0 0 0 2 291 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 6.21 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 177,065 Reads with adapters: 3,301 (1.9%) Reads written (passing filters): 177,065 (100.0%) Total basepairs processed: 48,837,319 bp Total written (filtered): 48,827,216 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 3301 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 61.1% C: 16.2% G: 17.4% T: 5.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 3141 2766.6 0 3141 4 126 691.7 0 126 5 29 172.9 0 29 6 4 43.2 0 4 7 1 10.8 0 1