This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCTTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 69.00 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 475,083 (18.1%) Reads that were too short: 149,214 (5.7%) Reads written (passing filters): 325,869 (12.4%) Total basepairs processed: 791,719,999 bp Total written (filtered): 93,636,018 bp (11.8%) === Adapter 1 === Sequence: GTCTTA; Type: regular 5'; Length: 6; Trimmed: 475083 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1839 642.2 0 1839 7 4876 642.2 0 4876 8 164610 642.2 0 164610 9 137580 642.2 0 137580 10 144 642.2 0 144 11 23 642.2 0 23 12 23 642.2 0 23 13 12 642.2 0 12 14 18 642.2 0 18 15 17 642.2 0 17 16 16 642.2 0 16 17 11 642.2 0 11 18 12 642.2 0 12 19 15 642.2 0 15 20 13 642.2 0 13 21 87 642.2 0 87 22 15 642.2 0 15 23 16 642.2 0 16 24 14 642.2 0 14 25 19 642.2 0 19 26 19 642.2 0 19 27 12 642.2 0 12 28 27 642.2 0 27 29 15 642.2 0 15 30 9 642.2 0 9 31 22 642.2 0 22 32 22 642.2 0 22 33 16 642.2 0 16 34 10 642.2 0 10 35 16 642.2 0 16 36 27 642.2 0 27 37 16 642.2 0 16 38 52 642.2 0 52 39 34 642.2 0 34 40 23 642.2 0 23 41 50 642.2 0 50 42 50 642.2 0 50 43 1295 642.2 0 1295 44 54 642.2 0 54 45 20 642.2 0 20 46 23 642.2 0 23 47 11 642.2 0 11 48 17 642.2 0 17 49 43 642.2 0 43 50 29 642.2 0 29 51 29 642.2 0 29 52 30 642.2 0 30 53 20 642.2 0 20 54 22 642.2 0 22 55 20 642.2 0 20 56 418 642.2 0 418 57 93 642.2 0 93 58 22 642.2 0 22 59 38 642.2 0 38 60 22 642.2 0 22 61 50 642.2 0 50 62 24 642.2 0 24 63 19 642.2 0 19 64 43 642.2 0 43 65 30 642.2 0 30 66 14 642.2 0 14 67 33 642.2 0 33 68 45 642.2 0 45 69 130 642.2 0 130 70 123 642.2 0 123 71 104 642.2 0 104 72 40 642.2 0 40 73 36 642.2 0 36 74 35 642.2 0 35 75 45 642.2 0 45 76 60 642.2 0 60 77 28 642.2 0 28 78 27 642.2 0 27 79 34 642.2 0 34 80 40 642.2 0 40 81 27 642.2 0 27 82 43 642.2 0 43 83 48 642.2 0 48 84 48 642.2 0 48 85 45 642.2 0 45 86 49 642.2 0 49 87 62 642.2 0 62 88 100 642.2 0 100 89 2581 642.2 0 2581 90 597 642.2 0 597 91 2252 642.2 0 2252 92 47 642.2 0 47 93 30 642.2 0 30 94 27 642.2 0 27 95 17 642.2 0 17 96 14 642.2 0 14 97 44 642.2 0 44 98 40 642.2 0 40 99 33 642.2 0 33 100 39 642.2 0 39 101 26 642.2 0 26 102 26 642.2 0 26 103 25 642.2 0 25 104 20 642.2 0 20 105 30 642.2 0 30 106 20 642.2 0 20 107 18 642.2 0 18 108 32 642.2 0 32 109 29 642.2 0 29 110 31 642.2 0 31 111 44 642.2 0 44 112 35 642.2 0 35 113 15 642.2 0 15 114 15 642.2 0 15 115 16 642.2 0 16 116 55 642.2 0 55 117 26 642.2 0 26 118 37 642.2 0 37 119 93 642.2 0 93 120 23 642.2 0 23 121 21 642.2 0 21 122 16 642.2 0 16 123 18 642.2 0 18 124 13 642.2 0 13 125 22 642.2 0 22 126 16 642.2 0 16 127 20 642.2 0 20 128 20 642.2 0 20 129 22 642.2 0 22 130 18 642.2 0 18 131 24 642.2 0 24 132 28 642.2 0 28 133 18 642.2 0 18 134 17 642.2 0 17 135 27 642.2 0 27 136 39 642.2 0 39 137 295 642.2 0 295 138 74 642.2 0 74 139 44 642.2 0 44 140 29 642.2 0 29 141 20 642.2 0 20 142 18 642.2 0 18 143 31 642.2 0 31 144 28 642.2 0 28 145 28 642.2 0 28 146 16 642.2 0 16 147 16 642.2 0 16 148 65 642.2 0 65 149 42 642.2 0 42 150 110 642.2 0 110 151 25 642.2 0 25 152 22 642.2 0 22 153 20 642.2 0 20 154 26 642.2 0 26 155 46 642.2 0 46 156 233 642.2 0 233 157 43 642.2 0 43 158 93 642.2 0 93 159 40 642.2 0 40 160 18 642.2 0 18 161 21 642.2 0 21 162 41 642.2 0 41 163 19 642.2 0 19 164 19 642.2 0 19 165 31 642.2 0 31 166 22 642.2 0 22 167 26 642.2 0 26 168 206 642.2 0 206 169 98 642.2 0 98 170 73 642.2 0 73 171 20 642.2 0 20 172 20 642.2 0 20 173 39 642.2 0 39 174 51 642.2 0 51 175 89 642.2 0 89 176 913 642.2 0 913 177 1857 642.2 0 1857 178 375 642.2 0 375 179 28 642.2 0 28 180 21 642.2 0 21 181 12 642.2 0 12 182 31 642.2 0 31 183 29 642.2 0 29 184 26 642.2 0 26 185 16 642.2 0 16 186 27 642.2 0 27 187 23 642.2 0 23 188 16 642.2 0 16 189 36 642.2 0 36 190 20 642.2 0 20 191 13 642.2 0 13 192 23 642.2 0 23 193 22 642.2 0 22 194 24 642.2 0 24 195 20 642.2 0 20 196 25 642.2 0 25 197 17 642.2 0 17 198 25 642.2 0 25 199 20 642.2 0 20 200 24 642.2 0 24 201 25 642.2 0 25 202 78 642.2 0 78 203 61 642.2 0 61 204 38 642.2 0 38 205 35 642.2 0 35 206 34 642.2 0 34 207 113 642.2 0 113 208 120 642.2 0 120 209 87 642.2 0 87 210 65 642.2 0 65 211 59 642.2 0 59 212 73 642.2 0 73 213 31 642.2 0 31 214 35 642.2 0 35 215 37 642.2 0 37 216 55 642.2 0 55 217 46 642.2 0 46 218 68 642.2 0 68 219 175 642.2 0 175 220 2020 642.2 0 2020 221 1091 642.2 0 1091 222 556 642.2 0 556 223 435 642.2 0 435 224 29 642.2 0 29 225 42 642.2 0 42 226 26 642.2 0 26 227 38 642.2 0 38 228 27 642.2 0 27 229 22 642.2 0 22 230 48 642.2 0 48 231 55 642.2 0 55 232 76 642.2 0 76 233 61 642.2 0 61 234 42 642.2 0 42 235 54 642.2 0 54 236 25 642.2 0 25 237 34 642.2 0 34 238 62 642.2 0 62 239 57 642.2 0 57 240 63 642.2 0 63 241 83 642.2 0 83 242 80 642.2 0 80 243 80 642.2 0 80 244 156 642.2 0 156 245 180 642.2 0 180 246 98 642.2 0 98 247 291 642.2 0 291 248 53 642.2 0 53 249 66 642.2 0 66 250 56 642.2 0 56 251 78 642.2 0 78 252 59 642.2 0 59 253 74 642.2 0 74 254 89 642.2 0 89 255 99 642.2 0 99 256 130 642.2 0 130 257 187 642.2 0 187 258 57 642.2 0 57 259 286 642.2 0 286 260 58 642.2 0 58 261 93 642.2 0 93 262 124 642.2 0 124 263 153 642.2 0 153 264 190 642.2 0 190 265 104 642.2 0 104 266 188 642.2 0 188 267 95 642.2 0 95 268 200 642.2 0 200 269 353 642.2 0 353 270 548 642.2 0 548 271 1154 642.2 0 1154 272 170 642.2 0 170 273 94 642.2 0 94 274 819 642.2 0 819 275 520 642.2 0 520 276 1075 642.2 0 1075 277 226 642.2 0 226 278 806 642.2 0 806 279 822 642.2 0 822 280 2047 642.2 0 2047 281 6358 642.2 0 6358 282 27714 642.2 0 27714 283 32123 642.2 0 32123 284 44079 642.2 0 44079 285 15151 642.2 0 15151 286 4404 642.2 0 4404 287 131 642.2 0 131 288 131 642.2 0 131 289 317 642.2 0 317 290 130 642.2 0 130 291 107 642.2 0 107 292 87 642.2 0 87 293 43 642.2 0 43 294 28 642.2 0 28 295 32 642.2 0 32 296 43 642.2 0 43 297 68 642.2 0 68 298 59 642.2 0 59 299 55 642.2 0 55 300 31 642.2 0 31 301 9 642.2 0 9 Finished in 15.89 s (49 us/read; 1.23 M reads/minute). === Summary === Total reads processed: 325,869 Reads with adapters: 144,548 (44.4%) Reads that were too short: 105 (0.0%) Reads written (passing filters): 144,443 (44.3%) Total basepairs processed: 93,636,018 bp Total written (filtered): 39,007,388 bp (41.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 144548 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 10 0.0 3 0 0 9 1 19 321 0.0 3 0 310 6 3 2 20 140919 0.0 4 138855 1984 69 9 2 21 315 0.0 4 39 271 5 22 18 0.0 4 1 1 16 23 93 0.0 4 2 0 1 85 5 24 7 0.0 4 0 0 0 0 7 27 27 0.0 4 0 0 0 0 27 28 1 0.0 4 1 29 2 0.0 4 2 32 1 0.0 4 1 34 1 0.0 4 0 0 0 0 1 35 40 0.0 4 0 1 0 0 39 37 1 0.0 4 1 41 1 0.0 4 1 44 1 0.0 4 0 0 0 0 1 45 2 0.0 4 2 59 1 0.0 4 0 0 0 0 1 74 9 0.0 4 0 0 0 0 9 83 6 0.0 4 0 0 0 0 6 85 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 101 2 0.0 4 0 1 0 0 1 110 1 0.0 4 0 0 0 0 1 111 1 0.0 4 0 0 0 0 1 112 2 0.0 4 1 0 0 0 1 125 5 0.0 4 0 1 0 0 4 126 10 0.0 4 0 0 0 0 10 127 2582 0.0 4 0 0 0 0 2582 128 21 0.0 4 1 0 0 0 20 130 4 0.0 4 0 0 0 0 4 138 2 0.0 4 2 139 1 0.0 4 1 142 1 0.0 4 0 0 0 0 1 146 2 0.0 4 0 0 0 0 2 153 11 0.0 4 0 0 0 0 11 154 2 0.0 4 2 156 1 0.0 4 1 157 1 0.0 4 1 159 3 0.0 4 3 160 2 0.0 4 2 166 2 0.0 4 1 0 0 1 168 1 0.0 4 0 0 0 0 1 170 1 0.0 4 0 0 0 0 1 171 1 0.0 4 1 172 3 0.0 4 0 0 0 0 3 173 1 0.0 4 0 0 0 0 1 178 1 0.0 4 0 0 0 0 1 180 1 0.0 4 1 188 2 0.0 4 1 0 0 0 1 194 2 0.0 4 0 0 0 0 2 202 1 0.0 4 1 203 1 0.0 4 1 204 3 0.0 4 2 1 206 2 0.0 4 1 0 0 0 1 207 1 0.0 4 0 0 0 0 1 209 1 0.0 4 0 0 0 0 1 210 1 0.0 4 1 214 2 0.0 4 0 0 0 0 2 218 2 0.0 4 0 1 0 0 1 219 1 0.0 4 0 0 0 0 1 223 3 0.0 4 0 0 0 0 3 225 2 0.0 4 0 1 0 1 226 1 0.0 4 1 227 4 0.0 4 0 3 1 228 4 0.0 4 0 0 0 0 4 230 1 0.0 4 0 0 0 0 1 232 3 0.0 4 1 0 0 0 2 233 1 0.0 4 0 0 0 0 1 234 3 0.0 4 0 1 0 0 2 235 1 0.0 4 0 0 0 0 1 236 1 0.0 4 0 0 0 0 1 237 3 0.0 4 0 0 0 0 3 238 1 0.0 4 0 0 0 0 1 239 2 0.0 4 1 1 241 1 0.0 4 0 1 249 1 0.0 4 0 0 0 0 1 254 1 0.0 4 0 0 0 0 1 255 5 0.0 4 0 0 0 0 5 257 1 0.0 4 0 0 1 258 2 0.0 4 1 0 0 0 1 264 1 0.0 4 1 267 1 0.0 4 0 0 1 270 1 0.0 4 0 0 0 0 1 272 2 0.0 4 0 0 0 1 1 274 1 0.0 4 0 0 0 0 1 275 7 0.0 4 2 0 0 1 4 276 1 0.0 4 0 1 277 1 0.0 4 0 0 0 0 1 279 1 0.0 4 1 281 2 0.0 4 1 0 0 0 1 282 1 0.0 4 0 0 0 0 1 283 9 0.0 4 5 2 1 1 284 7 0.0 4 0 2 2 1 2 285 3 0.0 4 0 2 1 286 2 0.0 4 1 0 0 1 287 1 0.0 4 0 0 0 0 1 288 2 0.0 4 1 0 0 0 1 293 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 5.12 s (35 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 144,443 Reads with adapters: 20,542 (14.2%) Reads written (passing filters): 144,443 (100.0%) Total basepairs processed: 39,007,388 bp Total written (filtered): 38,740,478 bp (99.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 20542 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.5% C: 4.1% G: 92.9% T: 1.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 71 2256.9 0 71 4 32 564.2 0 32 5 9 141.1 0 9 7 2 8.8 0 2 8 3 2.2 0 3 9 9385 0.6 0 300 9085 10 9482 0.1 1 314 9168 11 1036 0.0 1 15 1021 12 14 0.0 1 0 14 13 14 0.0 1 1 13 18 15 0.0 1 12 3 19 77 0.0 1 55 22 20 1 0.0 1 0 1 21 2 0.0 1 2 22 18 0.0 1 13 5 25 1 0.0 1 1 39 1 0.0 1 1 42 1 0.0 1 1 56 3 0.0 1 2 1 58 1 0.0 1 1 59 1 0.0 1 1 67 1 0.0 1 1 68 1 0.0 1 1 69 1 0.0 1 1 70 1 0.0 1 1 74 2 0.0 1 2 77 1 0.0 1 1 79 1 0.0 1 0 1 80 23 0.0 1 19 4 81 1 0.0 1 1 83 1 0.0 1 1 89 1 0.0 1 1 91 1 0.0 1 1 94 1 0.0 1 1 95 1 0.0 1 1 97 1 0.0 1 1 102 1 0.0 1 0 1 103 1 0.0 1 1 104 1 0.0 1 0 1 105 1 0.0 1 0 1 106 7 0.0 1 3 4 107 4 0.0 1 4 112 1 0.0 1 0 1 114 39 0.0 1 37 2 118 4 0.0 1 4 120 1 0.0 1 1 121 1 0.0 1 1 126 8 0.0 1 7 1 144 1 0.0 1 1 145 5 0.0 1 5 148 1 0.0 1 1 150 6 0.0 1 0 6 152 1 0.0 1 0 1 155 1 0.0 1 1 156 26 0.0 1 26 158 1 0.0 1 1 160 1 0.0 1 1 162 2 0.0 1 2 169 1 0.0 1 1 189 2 0.0 1 1 1 196 3 0.0 1 3 200 1 0.0 1 1 207 1 0.0 1 1 208 31 0.0 1 18 13 209 2 0.0 1 1 1 220 1 0.0 1 1 223 4 0.0 1 4 224 1 0.0 1 1 228 2 0.0 1 2 229 1 0.0 1 1 235 1 0.0 1 1 256 2 0.0 1 2 261 159 0.0 1 145 14 262 5 0.0 1 5 263 2 0.0 1 2 264 1 0.0 1 1 265 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.