This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGGATG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 70.24 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 263,470 (10.0%) Reads that were too short: 25,844 (1.0%) Reads written (passing filters): 237,626 (9.0%) Total basepairs processed: 791,719,999 bp Total written (filtered): 59,768,996 bp (7.5%) === Adapter 1 === Sequence: AGGATG; Type: regular 5'; Length: 6; Trimmed: 263470 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1879 642.2 0 1879 7 83268 642.2 0 83268 8 68976 642.2 0 68976 9 142 642.2 0 142 10 92 642.2 0 92 11 93 642.2 0 93 12 93 642.2 0 93 13 91 642.2 0 91 14 107 642.2 0 107 15 75 642.2 0 75 16 79 642.2 0 79 17 110 642.2 0 110 18 164 642.2 0 164 19 84 642.2 0 84 20 169 642.2 0 169 21 88 642.2 0 88 22 84 642.2 0 84 23 103 642.2 0 103 24 90 642.2 0 90 25 76 642.2 0 76 26 75 642.2 0 75 27 97 642.2 0 97 28 70 642.2 0 70 29 111 642.2 0 111 30 58 642.2 0 58 31 81 642.2 0 81 32 90 642.2 0 90 33 146 642.2 0 146 34 111 642.2 0 111 35 186 642.2 0 186 36 72 642.2 0 72 37 100 642.2 0 100 38 62 642.2 0 62 39 90 642.2 0 90 40 88 642.2 0 88 41 74 642.2 0 74 42 92 642.2 0 92 43 85 642.2 0 85 44 64 642.2 0 64 45 115 642.2 0 115 46 86 642.2 0 86 47 104 642.2 0 104 48 77 642.2 0 77 49 149 642.2 0 149 50 427 642.2 0 427 51 79 642.2 0 79 52 99 642.2 0 99 53 89 642.2 0 89 54 93 642.2 0 93 55 108 642.2 0 108 56 126 642.2 0 126 57 88 642.2 0 88 58 134 642.2 0 134 59 108 642.2 0 108 60 105 642.2 0 105 61 83 642.2 0 83 62 106 642.2 0 106 63 120 642.2 0 120 64 129 642.2 0 129 65 175 642.2 0 175 66 227 642.2 0 227 67 410 642.2 0 410 68 443 642.2 0 443 69 208 642.2 0 208 70 209 642.2 0 209 71 129 642.2 0 129 72 151 642.2 0 151 73 180 642.2 0 180 74 142 642.2 0 142 75 182 642.2 0 182 76 197 642.2 0 197 77 280 642.2 0 280 78 428 642.2 0 428 79 700 642.2 0 700 80 9299 642.2 0 9299 81 6486 642.2 0 6486 82 12252 642.2 0 12252 83 196 642.2 0 196 84 136 642.2 0 136 85 116 642.2 0 116 86 150 642.2 0 150 87 142 642.2 0 142 88 306 642.2 0 306 89 101 642.2 0 101 90 58 642.2 0 58 91 61 642.2 0 61 92 57 642.2 0 57 93 83 642.2 0 83 94 93 642.2 0 93 95 141 642.2 0 141 96 172 642.2 0 172 97 106 642.2 0 106 98 52 642.2 0 52 99 100 642.2 0 100 100 62 642.2 0 62 101 46 642.2 0 46 102 79 642.2 0 79 103 92 642.2 0 92 104 70 642.2 0 70 105 76 642.2 0 76 106 57 642.2 0 57 107 70 642.2 0 70 108 72 642.2 0 72 109 62 642.2 0 62 110 83 642.2 0 83 111 188 642.2 0 188 112 67 642.2 0 67 113 113 642.2 0 113 114 95 642.2 0 95 115 66 642.2 0 66 116 76 642.2 0 76 117 162 642.2 0 162 118 117 642.2 0 117 119 271 642.2 0 271 120 361 642.2 0 361 121 258 642.2 0 258 122 364 642.2 0 364 123 135 642.2 0 135 124 172 642.2 0 172 125 158 642.2 0 158 126 258 642.2 0 258 127 1284 642.2 0 1284 128 154 642.2 0 154 129 451 642.2 0 451 130 120 642.2 0 120 131 77 642.2 0 77 132 68 642.2 0 68 133 87 642.2 0 87 134 86 642.2 0 86 135 196 642.2 0 196 136 75 642.2 0 75 137 108 642.2 0 108 138 84 642.2 0 84 139 108 642.2 0 108 140 99 642.2 0 99 141 84 642.2 0 84 142 315 642.2 0 315 143 95 642.2 0 95 144 102 642.2 0 102 145 128 642.2 0 128 146 180 642.2 0 180 147 147 642.2 0 147 148 445 642.2 0 445 149 93 642.2 0 93 150 117 642.2 0 117 151 61 642.2 0 61 152 111 642.2 0 111 153 111 642.2 0 111 154 114 642.2 0 114 155 138 642.2 0 138 156 74 642.2 0 74 157 60 642.2 0 60 158 82 642.2 0 82 159 80 642.2 0 80 160 64 642.2 0 64 161 71 642.2 0 71 162 110 642.2 0 110 163 72 642.2 0 72 164 127 642.2 0 127 165 99 642.2 0 99 166 102 642.2 0 102 167 119 642.2 0 119 168 229 642.2 0 229 169 115 642.2 0 115 170 100 642.2 0 100 171 105 642.2 0 105 172 102 642.2 0 102 173 84 642.2 0 84 174 121 642.2 0 121 175 179 642.2 0 179 176 130 642.2 0 130 177 175 642.2 0 175 178 184 642.2 0 184 179 123 642.2 0 123 180 184 642.2 0 184 181 243 642.2 0 243 182 203 642.2 0 203 183 277 642.2 0 277 184 340 642.2 0 340 185 382 642.2 0 382 186 590 642.2 0 590 187 609 642.2 0 609 188 8896 642.2 0 8896 189 4761 642.2 0 4761 190 11383 642.2 0 11383 191 661 642.2 0 661 192 359 642.2 0 359 193 781 642.2 0 781 194 410 642.2 0 410 195 215 642.2 0 215 196 175 642.2 0 175 197 157 642.2 0 157 198 942 642.2 0 942 199 91 642.2 0 91 200 89 642.2 0 89 201 95 642.2 0 95 202 109 642.2 0 109 203 104 642.2 0 104 204 122 642.2 0 122 205 195 642.2 0 195 206 1417 642.2 0 1417 207 1094 642.2 0 1094 208 1657 642.2 0 1657 209 161 642.2 0 161 210 173 642.2 0 173 211 416 642.2 0 416 212 1130 642.2 0 1130 213 584 642.2 0 584 214 431 642.2 0 431 215 269 642.2 0 269 216 69 642.2 0 69 217 57 642.2 0 57 218 112 642.2 0 112 219 91 642.2 0 91 220 76 642.2 0 76 221 70 642.2 0 70 222 106 642.2 0 106 223 86 642.2 0 86 224 69 642.2 0 69 225 200 642.2 0 200 226 137 642.2 0 137 227 175 642.2 0 175 228 125 642.2 0 125 229 156 642.2 0 156 230 189 642.2 0 189 231 167 642.2 0 167 232 106 642.2 0 106 233 122 642.2 0 122 234 97 642.2 0 97 235 135 642.2 0 135 236 87 642.2 0 87 237 113 642.2 0 113 238 99 642.2 0 99 239 76 642.2 0 76 240 102 642.2 0 102 241 175 642.2 0 175 242 112 642.2 0 112 243 74 642.2 0 74 244 113 642.2 0 113 245 88 642.2 0 88 246 86 642.2 0 86 247 110 642.2 0 110 248 151 642.2 0 151 249 111 642.2 0 111 250 123 642.2 0 123 251 136 642.2 0 136 252 140 642.2 0 140 253 138 642.2 0 138 254 136 642.2 0 136 255 183 642.2 0 183 256 242 642.2 0 242 257 221 642.2 0 221 258 216 642.2 0 216 259 221 642.2 0 221 260 177 642.2 0 177 261 100 642.2 0 100 262 147 642.2 0 147 263 107 642.2 0 107 264 101 642.2 0 101 265 83 642.2 0 83 266 137 642.2 0 137 267 163 642.2 0 163 268 499 642.2 0 499 269 576 642.2 0 576 270 618 642.2 0 618 271 1195 642.2 0 1195 272 1435 642.2 0 1435 273 1338 642.2 0 1338 274 339 642.2 0 339 275 91 642.2 0 91 276 87 642.2 0 87 277 85 642.2 0 85 278 132 642.2 0 132 279 148 642.2 0 148 280 137 642.2 0 137 281 149 642.2 0 149 282 373 642.2 0 373 283 469 642.2 0 469 284 538 642.2 0 538 285 83 642.2 0 83 286 90 642.2 0 90 287 123 642.2 0 123 288 147 642.2 0 147 289 134 642.2 0 134 290 111 642.2 0 111 291 145 642.2 0 145 292 382 642.2 0 382 293 347 642.2 0 347 294 310 642.2 0 310 295 225 642.2 0 225 296 135 642.2 0 135 297 177 642.2 0 177 298 170 642.2 0 170 299 134 642.2 0 134 300 216 642.2 0 216 301 231 642.2 0 231 Finished in 10.07 s (42 us/read; 1.42 M reads/minute). === Summary === Total reads processed: 237,626 Reads with adapters: 81,948 (34.5%) Reads that were too short: 215 (0.1%) Reads written (passing filters): 81,733 (34.4%) Total basepairs processed: 59,768,996 bp Total written (filtered): 22,575,575 bp (37.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 81948 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 12 0.0 2 0 0 0 12 15 64 0.0 3 0 0 37 27 16 576 0.0 3 0 470 68 38 17 80135 0.0 3 73423 5423 1032 257 18 199 0.0 3 17 151 20 11 19 260 0.0 3 17 8 109 126 20 36 0.0 3 1 0 0 35 22 2 0.0 3 1 0 0 1 23 8 0.0 3 0 0 0 8 24 2 0.0 3 0 0 0 2 25 2 0.0 3 0 0 0 2 26 2 0.0 3 0 0 0 2 27 1 0.0 3 0 0 0 1 28 2 0.0 3 2 29 1 0.0 3 0 0 0 1 31 2 0.0 3 0 0 0 2 33 2 0.0 3 0 0 0 2 35 1 0.0 3 0 0 0 1 36 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 40 2 0.0 3 0 0 0 2 41 1 0.0 3 0 0 0 1 42 6 0.0 3 0 0 0 6 43 12 0.0 3 0 0 0 12 44 1 0.0 3 0 1 46 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 49 6 0.0 3 0 0 0 6 50 3 0.0 3 0 0 0 3 51 2 0.0 3 0 0 0 2 52 1 0.0 3 0 0 0 1 53 4 0.0 3 0 0 0 4 54 2 0.0 3 0 0 0 2 59 2 0.0 3 0 0 0 2 60 4 0.0 3 0 0 1 3 61 8 0.0 3 1 0 0 7 62 2 0.0 3 1 0 0 1 63 1 0.0 3 0 0 0 1 64 16 0.0 3 0 0 0 16 65 21 0.0 3 0 0 0 21 67 6 0.0 3 0 0 0 6 68 5 0.0 3 0 0 0 5 69 1 0.0 3 0 0 0 1 70 2 0.0 3 0 0 1 1 71 1 0.0 3 0 0 0 1 72 5 0.0 3 0 0 0 5 73 4 0.0 3 0 0 0 4 75 1 0.0 3 0 0 0 1 76 3 0.0 3 0 0 0 3 77 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 80 4 0.0 3 0 0 0 4 81 4 0.0 3 0 0 0 4 82 5 0.0 3 0 0 1 4 83 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 86 64 0.0 3 0 0 0 64 87 3 0.0 3 0 0 0 3 89 12 0.0 3 0 0 0 12 91 17 0.0 3 0 0 0 17 92 53 0.0 3 0 0 0 53 94 7 0.0 3 0 0 0 7 95 5 0.0 3 0 0 0 5 96 3 0.0 3 0 0 0 3 97 2 0.0 3 0 0 0 2 98 1 0.0 3 0 0 0 1 100 4 0.0 3 0 0 0 4 101 3 0.0 3 0 0 0 3 102 1 0.0 3 0 0 0 1 103 3 0.0 3 0 0 1 2 104 6 0.0 3 0 0 0 6 105 4 0.0 3 0 0 0 4 106 23 0.0 3 0 0 0 23 107 2 0.0 3 0 0 0 2 108 4 0.0 3 0 0 0 4 109 4 0.0 3 0 0 0 4 110 9 0.0 3 0 0 0 9 111 56 0.0 3 0 0 0 56 112 8 0.0 3 0 0 0 8 114 8 0.0 3 0 0 1 7 115 2 0.0 3 0 0 1 1 116 1 0.0 3 0 0 0 1 117 3 0.0 3 0 0 1 2 119 2 0.0 3 0 0 0 2 123 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 126 3 0.0 3 0 0 0 3 128 1 0.0 3 0 0 0 1 129 10 0.0 3 0 0 0 10 130 7 0.0 3 0 0 0 7 133 1 0.0 3 0 0 0 1 135 3 0.0 3 0 0 0 3 136 3 0.0 3 0 0 1 2 137 2 0.0 3 0 0 0 2 138 1 0.0 3 0 0 0 1 139 2 0.0 3 0 0 1 1 142 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 144 3 0.0 3 0 0 0 3 145 1 0.0 3 0 0 0 1 146 3 0.0 3 0 0 0 3 147 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 155 3 0.0 3 0 0 1 2 156 4 0.0 3 0 0 0 4 157 5 0.0 3 0 0 0 5 162 1 0.0 3 0 0 0 1 163 3 0.0 3 0 0 0 3 164 3 0.0 3 0 0 1 2 165 1 0.0 3 0 0 0 1 166 5 0.0 3 0 1 0 4 169 1 0.0 3 0 0 0 1 173 3 0.0 3 0 0 0 3 174 1 0.0 3 0 0 1 176 1 0.0 3 0 0 0 1 179 2 0.0 3 0 0 0 2 180 2 0.0 3 0 0 0 2 183 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 189 4 0.0 3 0 0 0 4 191 22 0.0 3 0 0 0 22 193 2 0.0 3 0 1 0 1 194 2 0.0 3 0 0 0 2 197 1 0.0 3 0 0 0 1 198 1 0.0 3 0 0 1 199 1 0.0 3 0 0 0 1 200 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 203 3 0.0 3 0 0 0 3 204 1 0.0 3 0 0 0 1 205 2 0.0 3 0 0 0 2 206 6 0.0 3 0 0 1 5 207 3 0.0 3 0 0 0 3 208 1 0.0 3 0 0 0 1 209 1 0.0 3 0 0 0 1 210 3 0.0 3 0 0 0 3 212 1 0.0 3 0 0 0 1 213 9 0.0 3 0 0 0 9 214 2 0.0 3 0 0 0 2 215 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 218 2 0.0 3 0 0 0 2 219 1 0.0 3 0 0 0 1 220 3 0.0 3 0 0 0 3 221 1 0.0 3 0 0 0 1 224 1 0.0 3 0 0 0 1 227 1 0.0 3 0 0 0 1 228 1 0.0 3 0 0 1 233 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 252 1 0.0 3 0 0 0 1 255 1 0.0 3 0 0 0 1 277 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 284 2 0.0 3 0 0 0 2 285 1 0.0 3 0 0 0 1 286 1 0.0 3 0 0 0 1 287 2 0.0 3 0 0 0 2 288 3 0.0 3 0 0 0 3 289 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.80 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 81,733 Reads with adapters: 98 (0.1%) Reads written (passing filters): 81,733 (100.0%) Total basepairs processed: 22,575,575 bp Total written (filtered): 22,575,221 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 98 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 31.6% C: 13.3% G: 31.6% T: 23.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 74 1277.1 0 74 4 16 319.3 0 16 5 2 79.8 0 2 6 1 20.0 0 1 9 1 0.3 0 0 1 10 1 0.1 1 0 1 11 3 0.0 1 0 3