This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGGATG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 69.94 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 255,506 (9.7%) Reads that were too short: 16,385 (0.6%) Reads written (passing filters): 239,121 (9.1%) Total basepairs processed: 791,719,999 bp Total written (filtered): 60,867,292 bp (7.7%) === Adapter 1 === Sequence: AGGATG; Type: regular 5'; Length: 6; Trimmed: 255506 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 996 642.2 0 996 7 77946 642.2 0 77946 8 82862 642.2 0 82862 9 111 642.2 0 111 10 61 642.2 0 61 11 54 642.2 0 54 12 44 642.2 0 44 13 33 642.2 0 33 14 54 642.2 0 54 15 48 642.2 0 48 16 45 642.2 0 45 17 34 642.2 0 34 18 64 642.2 0 64 19 65 642.2 0 65 20 84 642.2 0 84 21 65 642.2 0 65 22 68 642.2 0 68 23 62 642.2 0 62 24 46 642.2 0 46 25 51 642.2 0 51 26 63 642.2 0 63 27 68 642.2 0 68 28 55 642.2 0 55 29 111 642.2 0 111 30 69 642.2 0 69 31 80 642.2 0 80 32 65 642.2 0 65 33 131 642.2 0 131 34 79 642.2 0 79 35 168 642.2 0 168 36 54 642.2 0 54 37 81 642.2 0 81 38 50 642.2 0 50 39 76 642.2 0 76 40 75 642.2 0 75 41 73 642.2 0 73 42 77 642.2 0 77 43 85 642.2 0 85 44 46 642.2 0 46 45 97 642.2 0 97 46 51 642.2 0 51 47 78 642.2 0 78 48 154 642.2 0 154 49 101 642.2 0 101 50 423 642.2 0 423 51 57 642.2 0 57 52 94 642.2 0 94 53 86 642.2 0 86 54 68 642.2 0 68 55 73 642.2 0 73 56 82 642.2 0 82 57 60 642.2 0 60 58 68 642.2 0 68 59 91 642.2 0 91 60 83 642.2 0 83 61 66 642.2 0 66 62 101 642.2 0 101 63 102 642.2 0 102 64 122 642.2 0 122 65 131 642.2 0 131 66 232 642.2 0 232 67 422 642.2 0 422 68 663 642.2 0 663 69 194 642.2 0 194 70 189 642.2 0 189 71 112 642.2 0 112 72 108 642.2 0 108 73 119 642.2 0 119 74 143 642.2 0 143 75 137 642.2 0 137 76 160 642.2 0 160 77 224 642.2 0 224 78 423 642.2 0 423 79 516 642.2 0 516 80 9205 642.2 0 9205 81 6495 642.2 0 6495 82 12655 642.2 0 12655 83 94 642.2 0 94 84 61 642.2 0 61 85 52 642.2 0 52 86 61 642.2 0 61 87 118 642.2 0 118 88 226 642.2 0 226 89 65 642.2 0 65 90 44 642.2 0 44 91 53 642.2 0 53 92 43 642.2 0 43 93 45 642.2 0 45 94 50 642.2 0 50 95 71 642.2 0 71 96 73 642.2 0 73 97 61 642.2 0 61 98 55 642.2 0 55 99 62 642.2 0 62 100 51 642.2 0 51 101 32 642.2 0 32 102 51 642.2 0 51 103 67 642.2 0 67 104 56 642.2 0 56 105 41 642.2 0 41 106 61 642.2 0 61 107 55 642.2 0 55 108 61 642.2 0 61 109 71 642.2 0 71 110 75 642.2 0 75 111 210 642.2 0 210 112 57 642.2 0 57 113 84 642.2 0 84 114 56 642.2 0 56 115 56 642.2 0 56 116 55 642.2 0 55 117 84 642.2 0 84 118 52 642.2 0 52 119 129 642.2 0 129 120 134 642.2 0 134 121 112 642.2 0 112 122 189 642.2 0 189 123 69 642.2 0 69 124 76 642.2 0 76 125 83 642.2 0 83 126 121 642.2 0 121 127 1074 642.2 0 1074 128 105 642.2 0 105 129 378 642.2 0 378 130 130 642.2 0 130 131 63 642.2 0 63 132 50 642.2 0 50 133 70 642.2 0 70 134 73 642.2 0 73 135 184 642.2 0 184 136 74 642.2 0 74 137 63 642.2 0 63 138 66 642.2 0 66 139 59 642.2 0 59 140 62 642.2 0 62 141 74 642.2 0 74 142 331 642.2 0 331 143 95 642.2 0 95 144 69 642.2 0 69 145 108 642.2 0 108 146 158 642.2 0 158 147 86 642.2 0 86 148 453 642.2 0 453 149 52 642.2 0 52 150 101 642.2 0 101 151 59 642.2 0 59 152 70 642.2 0 70 153 69 642.2 0 69 154 47 642.2 0 47 155 97 642.2 0 97 156 26 642.2 0 26 157 48 642.2 0 48 158 57 642.2 0 57 159 60 642.2 0 60 160 57 642.2 0 57 161 61 642.2 0 61 162 69 642.2 0 69 163 72 642.2 0 72 164 112 642.2 0 112 165 96 642.2 0 96 166 76 642.2 0 76 167 83 642.2 0 83 168 204 642.2 0 204 169 100 642.2 0 100 170 74 642.2 0 74 171 108 642.2 0 108 172 86 642.2 0 86 173 58 642.2 0 58 174 79 642.2 0 79 175 162 642.2 0 162 176 98 642.2 0 98 177 132 642.2 0 132 178 164 642.2 0 164 179 83 642.2 0 83 180 124 642.2 0 124 181 213 642.2 0 213 182 206 642.2 0 206 183 243 642.2 0 243 184 300 642.2 0 300 185 348 642.2 0 348 186 471 642.2 0 471 187 465 642.2 0 465 188 8318 642.2 0 8318 189 4906 642.2 0 4906 190 12441 642.2 0 12441 191 428 642.2 0 428 192 177 642.2 0 177 193 367 642.2 0 367 194 179 642.2 0 179 195 110 642.2 0 110 196 121 642.2 0 121 197 133 642.2 0 133 198 995 642.2 0 995 199 60 642.2 0 60 200 58 642.2 0 58 201 67 642.2 0 67 202 66 642.2 0 66 203 61 642.2 0 61 204 77 642.2 0 77 205 91 642.2 0 91 206 963 642.2 0 963 207 812 642.2 0 812 208 1507 642.2 0 1507 209 42 642.2 0 42 210 89 642.2 0 89 211 128 642.2 0 128 212 287 642.2 0 287 213 212 642.2 0 212 214 126 642.2 0 126 215 320 642.2 0 320 216 61 642.2 0 61 217 66 642.2 0 66 218 113 642.2 0 113 219 70 642.2 0 70 220 56 642.2 0 56 221 56 642.2 0 56 222 100 642.2 0 100 223 61 642.2 0 61 224 44 642.2 0 44 225 177 642.2 0 177 226 82 642.2 0 82 227 154 642.2 0 154 228 70 642.2 0 70 229 54 642.2 0 54 230 76 642.2 0 76 231 80 642.2 0 80 232 42 642.2 0 42 233 60 642.2 0 60 234 57 642.2 0 57 235 51 642.2 0 51 236 54 642.2 0 54 237 81 642.2 0 81 238 47 642.2 0 47 239 43 642.2 0 43 240 49 642.2 0 49 241 89 642.2 0 89 242 66 642.2 0 66 243 45 642.2 0 45 244 71 642.2 0 71 245 47 642.2 0 47 246 45 642.2 0 45 247 63 642.2 0 63 248 73 642.2 0 73 249 52 642.2 0 52 250 64 642.2 0 64 251 92 642.2 0 92 252 86 642.2 0 86 253 110 642.2 0 110 254 75 642.2 0 75 255 80 642.2 0 80 256 114 642.2 0 114 257 100 642.2 0 100 258 101 642.2 0 101 259 138 642.2 0 138 260 72 642.2 0 72 261 62 642.2 0 62 262 87 642.2 0 87 263 76 642.2 0 76 264 60 642.2 0 60 265 61 642.2 0 61 266 78 642.2 0 78 267 82 642.2 0 82 268 131 642.2 0 131 269 175 642.2 0 175 270 190 642.2 0 190 271 1119 642.2 0 1119 272 1463 642.2 0 1463 273 1006 642.2 0 1006 274 175 642.2 0 175 275 49 642.2 0 49 276 39 642.2 0 39 277 67 642.2 0 67 278 76 642.2 0 76 279 82 642.2 0 82 280 82 642.2 0 82 281 113 642.2 0 113 282 196 642.2 0 196 283 249 642.2 0 249 284 441 642.2 0 441 285 67 642.2 0 67 286 61 642.2 0 61 287 85 642.2 0 85 288 87 642.2 0 87 289 78 642.2 0 78 290 96 642.2 0 96 291 107 642.2 0 107 292 292 642.2 0 292 293 280 642.2 0 280 294 198 642.2 0 198 295 164 642.2 0 164 296 93 642.2 0 93 297 69 642.2 0 69 298 79 642.2 0 79 299 59 642.2 0 59 300 115 642.2 0 115 301 128 642.2 0 128 Finished in 11.20 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 239,121 Reads with adapters: 85,563 (35.8%) Reads that were too short: 48 (0.0%) Reads written (passing filters): 85,515 (35.8%) Total basepairs processed: 60,867,292 bp Total written (filtered): 23,315,730 bp (38.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 85563 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 8 0.0 3 0 0 0 0 8 18 19 0.0 3 0 0 15 4 19 719 0.0 3 0 707 9 2 1 20 84216 0.0 4 82850 1320 41 5 21 197 0.0 4 40 155 2 22 8 0.0 4 2 0 5 0 1 23 41 0.0 4 1 0 0 37 3 24 6 0.0 4 1 0 0 0 5 25 1 0.0 4 1 30 1 0.0 4 1 39 1 0.0 4 0 0 0 0 1 53 2 0.0 4 0 0 0 0 2 55 19 0.0 4 0 0 0 0 19 62 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 1 83 1 0.0 4 0 1 114 1 0.0 4 1 123 1 0.0 4 0 0 0 0 1 126 7 0.0 4 0 0 0 0 7 127 267 0.0 4 0 0 0 0 267 130 2 0.0 4 0 0 0 0 2 153 1 0.0 4 0 0 0 0 1 154 1 0.0 4 0 0 0 0 1 155 16 0.0 4 0 0 0 0 16 156 7 0.0 4 0 0 0 0 7 157 3 0.0 4 0 0 0 1 2 162 1 0.0 4 0 0 0 0 1 174 1 0.0 4 1 186 1 0.0 4 0 0 0 0 1 195 2 0.0 4 2 197 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 224 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 230 1 0.0 4 0 0 0 0 1 233 1 0.0 4 1 244 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 284 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.07 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 85,515 Reads with adapters: 8,814 (10.3%) Reads written (passing filters): 85,515 (100.0%) Total basepairs processed: 23,315,730 bp Total written (filtered): 23,218,245 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 8814 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.3% C: 3.6% G: 92.8% T: 1.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 88 1336.2 0 88 4 229 334.0 0 229 5 5 83.5 0 5 6 14 20.9 0 14 7 12 5.2 0 12 8 14 1.3 0 14 9 161 0.3 0 28 133 10 272 0.1 1 16 256 11 7942 0.0 1 39 7903 12 33 0.0 1 0 33 13 21 0.0 1 0 21 14 3 0.0 1 1 2 33 1 0.0 1 1 82 1 0.0 1 1 84 1 0.0 1 1 110 1 0.0 1 1 170 1 0.0 1 1 204 3 0.0 1 3 211 1 0.0 1 1 214 1 0.0 1 1 224 9 0.0 1 9 226 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.