This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCAAGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 68.97 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 86,614 (3.3%) Reads that were too short: 23,395 (0.9%) Reads written (passing filters): 63,219 (2.4%) Total basepairs processed: 791,719,999 bp Total written (filtered): 11,919,252 bp (1.5%) === Adapter 1 === Sequence: CCAAGG; Type: regular 5'; Length: 6; Trimmed: 86614 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 163 642.2 0 163 7 260 642.2 0 260 8 6937 642.2 0 6937 9 5348 642.2 0 5348 10 16 642.2 0 16 11 25 642.2 0 25 12 7 642.2 0 7 13 42 642.2 0 42 14 187 642.2 0 187 15 335 642.2 0 335 16 51 642.2 0 51 17 24 642.2 0 24 18 63 642.2 0 63 19 54 642.2 0 54 20 43 642.2 0 43 21 14 642.2 0 14 22 3 642.2 0 3 23 7 642.2 0 7 24 12 642.2 0 12 25 6 642.2 0 6 26 6 642.2 0 6 27 26 642.2 0 26 28 22 642.2 0 22 29 12 642.2 0 12 30 10 642.2 0 10 31 11 642.2 0 11 32 30 642.2 0 30 33 12 642.2 0 12 34 17 642.2 0 17 35 14 642.2 0 14 36 10 642.2 0 10 37 10 642.2 0 10 38 12 642.2 0 12 39 13 642.2 0 13 40 13 642.2 0 13 41 4 642.2 0 4 42 19 642.2 0 19 43 142 642.2 0 142 44 419 642.2 0 419 45 484 642.2 0 484 46 166 642.2 0 166 47 34 642.2 0 34 48 6 642.2 0 6 49 9 642.2 0 9 50 90 642.2 0 90 51 21 642.2 0 21 52 54 642.2 0 54 53 34 642.2 0 34 54 11 642.2 0 11 55 114 642.2 0 114 56 16 642.2 0 16 57 22 642.2 0 22 58 45 642.2 0 45 59 22 642.2 0 22 60 49 642.2 0 49 61 25 642.2 0 25 62 13 642.2 0 13 63 11 642.2 0 11 64 70 642.2 0 70 65 21 642.2 0 21 66 20 642.2 0 20 67 65 642.2 0 65 68 62 642.2 0 62 69 55 642.2 0 55 70 100 642.2 0 100 71 31 642.2 0 31 72 57 642.2 0 57 73 56 642.2 0 56 74 69 642.2 0 69 75 69 642.2 0 69 76 377 642.2 0 377 77 2364 642.2 0 2364 78 1988 642.2 0 1988 79 1621 642.2 0 1621 80 36 642.2 0 36 81 189 642.2 0 189 82 21 642.2 0 21 83 18 642.2 0 18 84 64 642.2 0 64 85 28 642.2 0 28 86 35 642.2 0 35 87 8 642.2 0 8 88 15 642.2 0 15 89 18 642.2 0 18 90 43 642.2 0 43 91 65 642.2 0 65 92 62 642.2 0 62 93 85 642.2 0 85 94 52 642.2 0 52 95 40 642.2 0 40 96 29 642.2 0 29 97 22 642.2 0 22 98 16 642.2 0 16 99 58 642.2 0 58 100 95 642.2 0 95 101 156 642.2 0 156 102 28 642.2 0 28 103 143 642.2 0 143 104 71 642.2 0 71 105 27 642.2 0 27 106 46 642.2 0 46 107 56 642.2 0 56 108 39 642.2 0 39 109 54 642.2 0 54 110 64 642.2 0 64 111 93 642.2 0 93 112 112 642.2 0 112 113 209 642.2 0 209 114 3630 642.2 0 3630 115 492 642.2 0 492 116 8895 642.2 0 8895 117 91 642.2 0 91 118 30 642.2 0 30 119 47 642.2 0 47 120 39 642.2 0 39 121 20 642.2 0 20 122 123 642.2 0 123 123 34 642.2 0 34 124 33 642.2 0 33 125 32 642.2 0 32 126 62 642.2 0 62 127 62 642.2 0 62 128 36 642.2 0 36 129 27 642.2 0 27 130 29 642.2 0 29 131 16 642.2 0 16 132 20 642.2 0 20 133 39 642.2 0 39 134 43 642.2 0 43 135 121 642.2 0 121 136 174 642.2 0 174 137 127 642.2 0 127 138 46 642.2 0 46 139 125 642.2 0 125 140 54 642.2 0 54 141 31 642.2 0 31 142 42 642.2 0 42 143 40 642.2 0 40 144 52 642.2 0 52 145 19 642.2 0 19 146 18 642.2 0 18 147 17 642.2 0 17 148 23 642.2 0 23 149 27 642.2 0 27 150 55 642.2 0 55 151 291 642.2 0 291 152 50 642.2 0 50 153 19 642.2 0 19 154 38 642.2 0 38 155 50 642.2 0 50 156 81 642.2 0 81 157 22 642.2 0 22 158 86 642.2 0 86 159 18 642.2 0 18 160 34 642.2 0 34 161 35 642.2 0 35 162 26 642.2 0 26 163 65 642.2 0 65 164 128 642.2 0 128 165 178 642.2 0 178 166 65 642.2 0 65 167 167 642.2 0 167 168 45 642.2 0 45 169 94 642.2 0 94 170 34 642.2 0 34 171 34 642.2 0 34 172 106 642.2 0 106 173 697 642.2 0 697 174 1170 642.2 0 1170 175 1202 642.2 0 1202 176 121 642.2 0 121 177 33 642.2 0 33 178 53 642.2 0 53 179 135 642.2 0 135 180 1012 642.2 0 1012 181 510 642.2 0 510 182 476 642.2 0 476 183 78 642.2 0 78 184 51 642.2 0 51 185 155 642.2 0 155 186 74 642.2 0 74 187 92 642.2 0 92 188 104 642.2 0 104 189 159 642.2 0 159 190 960 642.2 0 960 191 6979 642.2 0 6979 192 4115 642.2 0 4115 193 2228 642.2 0 2228 194 1160 642.2 0 1160 195 65 642.2 0 65 196 47 642.2 0 47 197 37 642.2 0 37 198 62 642.2 0 62 199 183 642.2 0 183 200 33 642.2 0 33 201 44 642.2 0 44 202 269 642.2 0 269 203 987 642.2 0 987 204 637 642.2 0 637 205 265 642.2 0 265 206 35 642.2 0 35 207 312 642.2 0 312 208 135 642.2 0 135 209 421 642.2 0 421 210 196 642.2 0 196 211 83 642.2 0 83 212 16 642.2 0 16 213 20 642.2 0 20 214 17 642.2 0 17 215 21 642.2 0 21 216 63 642.2 0 63 217 66 642.2 0 66 218 70 642.2 0 70 219 34 642.2 0 34 220 46 642.2 0 46 221 27 642.2 0 27 222 45 642.2 0 45 223 29 642.2 0 29 224 19 642.2 0 19 225 38 642.2 0 38 226 38 642.2 0 38 227 40 642.2 0 40 228 18 642.2 0 18 229 33 642.2 0 33 230 53 642.2 0 53 231 50 642.2 0 50 232 45 642.2 0 45 233 36 642.2 0 36 234 94 642.2 0 94 235 54 642.2 0 54 236 55 642.2 0 55 237 39 642.2 0 39 238 33 642.2 0 33 239 20 642.2 0 20 240 47 642.2 0 47 241 27 642.2 0 27 242 35 642.2 0 35 243 44 642.2 0 44 244 36 642.2 0 36 245 60 642.2 0 60 246 43 642.2 0 43 247 27 642.2 0 27 248 39 642.2 0 39 249 35 642.2 0 35 250 40 642.2 0 40 251 133 642.2 0 133 252 387 642.2 0 387 253 354 642.2 0 354 254 464 642.2 0 464 255 100 642.2 0 100 256 51 642.2 0 51 257 63 642.2 0 63 258 352 642.2 0 352 259 360 642.2 0 360 260 1746 642.2 0 1746 261 912 642.2 0 912 262 625 642.2 0 625 263 386 642.2 0 386 264 459 642.2 0 459 265 668 642.2 0 668 266 386 642.2 0 386 267 87 642.2 0 87 268 44 642.2 0 44 269 30 642.2 0 30 270 45 642.2 0 45 271 65 642.2 0 65 272 244 642.2 0 244 273 93 642.2 0 93 274 83 642.2 0 83 275 63 642.2 0 63 276 37 642.2 0 37 277 89 642.2 0 89 278 84 642.2 0 84 279 133 642.2 0 133 280 121 642.2 0 121 281 118 642.2 0 118 282 73 642.2 0 73 283 228 642.2 0 228 284 1595 642.2 0 1595 285 2623 642.2 0 2623 286 2647 642.2 0 2647 287 776 642.2 0 776 288 52 642.2 0 52 289 78 642.2 0 78 290 55 642.2 0 55 291 58 642.2 0 58 292 80 642.2 0 80 293 44 642.2 0 44 294 91 642.2 0 91 295 482 642.2 0 482 296 176 642.2 0 176 297 323 642.2 0 323 298 97 642.2 0 97 299 51 642.2 0 51 300 45 642.2 0 45 301 147 642.2 0 147 Finished in 2.40 s (38 us/read; 1.58 M reads/minute). === Summary === Total reads processed: 63,219 Reads with adapters: 7,549 (11.9%) Reads that were too short: 214 (0.3%) Reads written (passing filters): 7,335 (11.6%) Total basepairs processed: 11,919,252 bp Total written (filtered): 1,925,296 bp (16.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 7549 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 10 0.0 3 0 0 5 5 16 76 0.0 3 0 54 15 7 17 6431 0.0 3 5857 454 93 27 18 131 0.0 3 8 12 8 103 19 21 0.0 3 0 1 7 13 20 93 0.0 3 59 6 2 26 21 25 0.0 3 0 0 2 23 22 3 0.0 3 0 0 1 2 27 2 0.0 3 0 0 0 2 28 1 0.0 3 0 0 0 1 29 2 0.0 3 0 0 0 2 30 4 0.0 3 0 0 0 4 31 1 0.0 3 0 0 0 1 33 5 0.0 3 0 0 0 5 34 2 0.0 3 0 0 0 2 35 2 0.0 3 0 0 0 2 39 2 0.0 3 0 0 0 2 40 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 43 3 0.0 3 0 0 0 3 44 4 0.0 3 0 0 0 4 45 1 0.0 3 0 0 0 1 48 2 0.0 3 0 0 0 2 50 2 0.0 3 1 0 0 1 52 4 0.0 3 0 0 0 4 53 3 0.0 3 0 0 0 3 54 5 0.0 3 0 0 0 5 55 1 0.0 3 0 0 0 1 56 3 0.0 3 0 0 0 3 57 300 0.0 3 0 0 8 292 58 53 0.0 3 0 0 2 51 59 3 0.0 3 0 0 1 2 60 53 0.0 3 0 0 4 49 61 12 0.0 3 0 0 0 12 62 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 66 2 0.0 3 0 0 0 2 68 2 0.0 3 0 0 0 2 69 4 0.0 3 0 0 0 4 70 2 0.0 3 0 0 0 2 71 2 0.0 3 0 0 0 2 72 1 0.0 3 0 0 1 73 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 78 4 0.0 3 0 0 1 3 81 2 0.0 3 0 0 0 2 83 9 0.0 3 0 0 0 9 84 5 0.0 3 0 0 0 5 85 2 0.0 3 0 0 0 2 86 2 0.0 3 0 0 0 2 87 2 0.0 3 0 0 0 2 88 1 0.0 3 0 0 0 1 89 3 0.0 3 0 0 0 3 93 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 95 5 0.0 3 0 0 0 5 96 1 0.0 3 0 0 0 1 97 3 0.0 3 0 0 0 3 98 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 101 2 0.0 3 0 1 0 1 104 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 107 2 0.0 3 0 0 0 2 108 5 0.0 3 0 0 1 4 109 4 0.0 3 0 0 1 3 110 7 0.0 3 1 0 0 6 111 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 113 2 0.0 3 0 0 0 2 117 2 0.0 3 0 0 0 2 118 2 0.0 3 0 0 0 2 119 9 0.0 3 0 0 0 9 123 2 0.0 3 0 0 0 2 124 4 0.0 3 0 0 1 3 125 2 0.0 3 0 0 1 1 126 1 0.0 3 0 0 0 1 127 2 0.0 3 0 0 0 2 128 6 0.0 3 0 0 0 6 129 1 0.0 3 0 0 0 1 130 3 0.0 3 0 0 0 3 131 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 134 2 0.0 3 0 0 0 2 136 1 0.0 3 0 0 0 1 138 2 0.0 3 0 0 1 1 139 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 147 6 0.0 3 0 0 0 6 148 1 0.0 3 0 0 0 1 150 10 0.0 3 0 0 1 9 151 2 0.0 3 0 0 0 2 152 2 0.0 3 0 0 0 2 153 1 0.0 3 0 0 0 1 157 3 0.0 3 0 0 0 3 158 10 0.0 3 0 0 0 10 159 1 0.0 3 0 0 0 1 160 2 0.0 3 0 0 0 2 161 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 165 1 0.0 3 0 1 167 3 0.0 3 0 0 0 3 168 1 0.0 3 0 0 0 1 169 24 0.0 3 0 0 0 24 170 8 0.0 3 0 0 0 8 171 2 0.0 3 0 0 0 2 172 18 0.0 3 0 0 0 18 173 8 0.0 3 0 0 0 8 174 1 0.0 3 0 0 0 1 177 4 0.0 3 0 0 0 4 178 3 0.0 3 0 0 0 3 180 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 194 3 0.0 3 0 0 0 3 198 1 0.0 3 0 0 0 1 200 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 209 2 0.0 3 0 0 0 2 210 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 219 1 0.0 3 0 0 0 1 220 1 0.0 3 0 0 0 1 221 2 0.0 3 0 0 0 2 222 1 0.0 3 0 0 0 1 229 1 0.0 3 0 0 0 1 230 1 0.0 3 0 0 0 1 231 1 0.0 3 0 0 0 1 240 1 0.0 3 0 0 0 1 241 1 0.0 3 0 0 1 243 1 0.0 3 0 0 0 1 249 1 0.0 3 0 0 0 1 254 1 0.0 3 0 0 0 1 257 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.25 s (34 us/read; 1.76 M reads/minute). === Summary === Total reads processed: 7,335 Reads with adapters: 40 (0.5%) Reads written (passing filters): 7,335 (100.0%) Total basepairs processed: 1,925,296 bp Total written (filtered): 1,925,122 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 40 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 47.5% C: 25.0% G: 22.5% T: 5.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 30 114.6 0 30 4 2 28.7 0 2 5 2 7.2 0 2 11 6 0.0 1 0 6