This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCAAGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 69.71 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 71,380 (2.7%) Reads that were too short: 10,729 (0.4%) Reads written (passing filters): 60,651 (2.3%) Total basepairs processed: 791,719,999 bp Total written (filtered): 11,494,835 bp (1.5%) === Adapter 1 === Sequence: CCAAGG; Type: regular 5'; Length: 6; Trimmed: 71380 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 162 642.2 0 162 7 226 642.2 0 226 8 6617 642.2 0 6617 9 6284 642.2 0 6284 10 4 642.2 0 4 11 3 642.2 0 3 12 3 642.2 0 3 13 4 642.2 0 4 14 4 642.2 0 4 15 8 642.2 0 8 16 1 642.2 0 1 17 6 642.2 0 6 18 16 642.2 0 16 19 7 642.2 0 7 20 14 642.2 0 14 21 12 642.2 0 12 22 4 642.2 0 4 23 5 642.2 0 5 24 9 642.2 0 9 25 9 642.2 0 9 26 1 642.2 0 1 27 5 642.2 0 5 28 2 642.2 0 2 29 4 642.2 0 4 30 9 642.2 0 9 31 9 642.2 0 9 32 36 642.2 0 36 33 11 642.2 0 11 34 8 642.2 0 8 35 7 642.2 0 7 36 2 642.2 0 2 37 8 642.2 0 8 38 6 642.2 0 6 39 8 642.2 0 8 40 6 642.2 0 6 41 9 642.2 0 9 42 14 642.2 0 14 43 17 642.2 0 17 44 36 642.2 0 36 45 65 642.2 0 65 46 27 642.2 0 27 47 31 642.2 0 31 48 7 642.2 0 7 49 14 642.2 0 14 50 87 642.2 0 87 51 9 642.2 0 9 52 59 642.2 0 59 53 20 642.2 0 20 54 12 642.2 0 12 55 138 642.2 0 138 56 11 642.2 0 11 57 19 642.2 0 19 58 45 642.2 0 45 59 16 642.2 0 16 60 22 642.2 0 22 61 6 642.2 0 6 62 17 642.2 0 17 63 9 642.2 0 9 64 62 642.2 0 62 65 19 642.2 0 19 66 18 642.2 0 18 67 25 642.2 0 25 68 10 642.2 0 10 69 37 642.2 0 37 70 56 642.2 0 56 71 20 642.2 0 20 72 48 642.2 0 48 73 36 642.2 0 36 74 41 642.2 0 41 75 55 642.2 0 55 76 223 642.2 0 223 77 2702 642.2 0 2702 78 2286 642.2 0 2286 79 1685 642.2 0 1685 80 18 642.2 0 18 81 181 642.2 0 181 82 33 642.2 0 33 83 10 642.2 0 10 84 25 642.2 0 25 85 7 642.2 0 7 86 10 642.2 0 10 87 8 642.2 0 8 88 13 642.2 0 13 89 22 642.2 0 22 90 35 642.2 0 35 91 33 642.2 0 33 92 26 642.2 0 26 93 46 642.2 0 46 94 45 642.2 0 45 95 16 642.2 0 16 96 16 642.2 0 16 97 20 642.2 0 20 98 29 642.2 0 29 99 50 642.2 0 50 100 94 642.2 0 94 101 174 642.2 0 174 102 33 642.2 0 33 103 134 642.2 0 134 104 97 642.2 0 97 105 37 642.2 0 37 106 49 642.2 0 49 107 52 642.2 0 52 108 48 642.2 0 48 109 54 642.2 0 54 110 57 642.2 0 57 111 86 642.2 0 86 112 101 642.2 0 101 113 173 642.2 0 173 114 4357 642.2 0 4357 115 380 642.2 0 380 116 10398 642.2 0 10398 117 78 642.2 0 78 118 24 642.2 0 24 119 36 642.2 0 36 120 37 642.2 0 37 121 17 642.2 0 17 122 122 642.2 0 122 123 18 642.2 0 18 124 31 642.2 0 31 125 6 642.2 0 6 126 11 642.2 0 11 127 16 642.2 0 16 128 10 642.2 0 10 129 3 642.2 0 3 130 14 642.2 0 14 131 10 642.2 0 10 132 6 642.2 0 6 133 34 642.2 0 34 134 17 642.2 0 17 135 57 642.2 0 57 136 103 642.2 0 103 137 49 642.2 0 49 138 20 642.2 0 20 139 137 642.2 0 137 140 42 642.2 0 42 141 12 642.2 0 12 142 11 642.2 0 11 143 56 642.2 0 56 144 15 642.2 0 15 145 11 642.2 0 11 146 12 642.2 0 12 147 9 642.2 0 9 148 9 642.2 0 9 149 13 642.2 0 13 150 28 642.2 0 28 151 305 642.2 0 305 152 46 642.2 0 46 153 9 642.2 0 9 154 22 642.2 0 22 155 20 642.2 0 20 156 85 642.2 0 85 157 14 642.2 0 14 158 82 642.2 0 82 159 15 642.2 0 15 160 10 642.2 0 10 161 20 642.2 0 20 162 10 642.2 0 10 163 30 642.2 0 30 164 48 642.2 0 48 165 103 642.2 0 103 166 10 642.2 0 10 167 153 642.2 0 153 168 16 642.2 0 16 169 89 642.2 0 89 170 26 642.2 0 26 171 16 642.2 0 16 172 41 642.2 0 41 173 206 642.2 0 206 174 427 642.2 0 427 175 440 642.2 0 440 176 99 642.2 0 99 177 14 642.2 0 14 178 36 642.2 0 36 179 74 642.2 0 74 180 489 642.2 0 489 181 221 642.2 0 221 182 183 642.2 0 183 183 70 642.2 0 70 184 23 642.2 0 23 185 42 642.2 0 42 186 49 642.2 0 49 187 68 642.2 0 68 188 92 642.2 0 92 189 165 642.2 0 165 190 865 642.2 0 865 191 8577 642.2 0 8577 192 4789 642.2 0 4789 193 2037 642.2 0 2037 194 621 642.2 0 621 195 23 642.2 0 23 196 55 642.2 0 55 197 12 642.2 0 12 198 33 642.2 0 33 199 162 642.2 0 162 200 13 642.2 0 13 201 22 642.2 0 22 202 192 642.2 0 192 203 355 642.2 0 355 204 209 642.2 0 209 205 138 642.2 0 138 206 23 642.2 0 23 207 356 642.2 0 356 208 25 642.2 0 25 209 43 642.2 0 43 210 24 642.2 0 24 211 18 642.2 0 18 212 6 642.2 0 6 213 8 642.2 0 8 214 4 642.2 0 4 215 5 642.2 0 5 216 14 642.2 0 14 217 20 642.2 0 20 218 12 642.2 0 12 219 8 642.2 0 8 220 7 642.2 0 7 221 7 642.2 0 7 222 6 642.2 0 6 223 4 642.2 0 4 224 13 642.2 0 13 225 8 642.2 0 8 226 8 642.2 0 8 227 11 642.2 0 11 228 14 642.2 0 14 229 8 642.2 0 8 230 53 642.2 0 53 231 18 642.2 0 18 232 10 642.2 0 10 233 16 642.2 0 16 234 35 642.2 0 35 235 16 642.2 0 16 236 19 642.2 0 19 237 19 642.2 0 19 238 14 642.2 0 14 239 30 642.2 0 30 240 36 642.2 0 36 241 16 642.2 0 16 242 9 642.2 0 9 243 9 642.2 0 9 244 9 642.2 0 9 245 33 642.2 0 33 246 49 642.2 0 49 247 14 642.2 0 14 248 19 642.2 0 19 249 10 642.2 0 10 250 22 642.2 0 22 251 53 642.2 0 53 252 126 642.2 0 126 253 131 642.2 0 131 254 179 642.2 0 179 255 30 642.2 0 30 256 30 642.2 0 30 257 33 642.2 0 33 258 156 642.2 0 156 259 297 642.2 0 297 260 890 642.2 0 890 261 514 642.2 0 514 262 224 642.2 0 224 263 102 642.2 0 102 264 206 642.2 0 206 265 229 642.2 0 229 266 195 642.2 0 195 267 21 642.2 0 21 268 16 642.2 0 16 269 17 642.2 0 17 270 22 642.2 0 22 271 31 642.2 0 31 272 111 642.2 0 111 273 34 642.2 0 34 274 32 642.2 0 32 275 22 642.2 0 22 276 19 642.2 0 19 277 28 642.2 0 28 278 27 642.2 0 27 279 45 642.2 0 45 280 28 642.2 0 28 281 31 642.2 0 31 282 37 642.2 0 37 283 49 642.2 0 49 284 615 642.2 0 615 285 1222 642.2 0 1222 286 1098 642.2 0 1098 287 1167 642.2 0 1167 288 30 642.2 0 30 289 39 642.2 0 39 290 25 642.2 0 25 291 25 642.2 0 25 292 20 642.2 0 20 293 13 642.2 0 13 294 22 642.2 0 22 295 144 642.2 0 144 296 60 642.2 0 60 297 161 642.2 0 161 298 23 642.2 0 23 299 12 642.2 0 12 300 19 642.2 0 19 301 67 642.2 0 67 Finished in 2.54 s (42 us/read; 1.43 M reads/minute). === Summary === Total reads processed: 60,651 Reads with adapters: 6,895 (11.4%) Reads that were too short: 16 (0.0%) Reads written (passing filters): 6,879 (11.3%) Total basepairs processed: 11,494,835 bp Total written (filtered): 1,871,639 bp (16.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 6895 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 1 2 18 4 0.0 3 0 0 4 19 128 0.0 3 0 126 2 20 6709 0.0 4 6613 92 3 1 21 22 0.0 4 5 15 1 1 22 3 0.0 4 0 0 3 23 5 0.0 4 0 0 0 4 1 24 3 0.0 4 0 0 0 0 3 27 1 0.0 4 1 31 1 0.0 4 0 0 0 0 1 64 1 0.0 4 1 70 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 0 0 0 1 111 1 0.0 4 0 0 0 0 1 117 2 0.0 4 1 0 0 0 1 128 1 0.0 4 1 157 1 0.0 4 0 0 0 0 1 167 2 0.0 4 2 191 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 229 3 0.0 4 0 0 0 0 3 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.24 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 6,879 Reads with adapters: 860 (12.5%) Reads written (passing filters): 6,879 (100.0%) Total basepairs processed: 1,871,639 bp Total written (filtered): 1,863,267 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 860 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.7% C: 6.2% G: 90.7% T: 1.4% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 2 107.5 0 2 9 323 0.0 0 10 313 10 426 0.0 1 16 410 11 109 0.0 1 1 108 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.