This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACATTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 67.26 s (26 us/read; 2.35 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 91,423 (3.5%) Reads that were too short: 14,009 (0.5%) Reads written (passing filters): 77,414 (2.9%) Total basepairs processed: 791,719,999 bp Total written (filtered): 21,378,302 bp (2.7%) === Adapter 1 === Sequence: ACATTG; Type: regular 5'; Length: 6; Trimmed: 91423 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 670 642.2 0 670 7 34994 642.2 0 34994 8 28478 642.2 0 28478 9 28 642.2 0 28 10 22 642.2 0 22 11 7 642.2 0 7 12 10 642.2 0 10 13 24 642.2 0 24 14 27 642.2 0 27 15 30 642.2 0 30 16 18 642.2 0 18 17 16 642.2 0 16 18 84 642.2 0 84 19 67 642.2 0 67 20 70 642.2 0 70 21 15 642.2 0 15 22 20 642.2 0 20 23 17 642.2 0 17 24 14 642.2 0 14 25 24 642.2 0 24 26 32 642.2 0 32 27 42 642.2 0 42 28 38 642.2 0 38 29 32 642.2 0 32 30 21 642.2 0 21 31 10 642.2 0 10 32 15 642.2 0 15 33 47 642.2 0 47 34 33 642.2 0 33 35 57 642.2 0 57 36 179 642.2 0 179 37 162 642.2 0 162 38 567 642.2 0 567 39 23 642.2 0 23 40 33 642.2 0 33 41 41 642.2 0 41 42 24 642.2 0 24 43 396 642.2 0 396 44 450 642.2 0 450 45 969 642.2 0 969 46 34 642.2 0 34 47 20 642.2 0 20 48 23 642.2 0 23 49 31 642.2 0 31 50 30 642.2 0 30 51 28 642.2 0 28 52 30 642.2 0 30 53 24 642.2 0 24 54 23 642.2 0 23 55 30 642.2 0 30 56 38 642.2 0 38 57 47 642.2 0 47 58 26 642.2 0 26 59 21 642.2 0 21 60 22 642.2 0 22 61 28 642.2 0 28 62 14 642.2 0 14 63 26 642.2 0 26 64 45 642.2 0 45 65 15 642.2 0 15 66 21 642.2 0 21 67 22 642.2 0 22 68 33 642.2 0 33 69 33 642.2 0 33 70 18 642.2 0 18 71 33 642.2 0 33 72 33 642.2 0 33 73 40 642.2 0 40 74 30 642.2 0 30 75 35 642.2 0 35 76 32 642.2 0 32 77 36 642.2 0 36 78 34 642.2 0 34 79 41 642.2 0 41 80 37 642.2 0 37 81 24 642.2 0 24 82 40 642.2 0 40 83 27 642.2 0 27 84 27 642.2 0 27 85 22 642.2 0 22 86 30 642.2 0 30 87 32 642.2 0 32 88 28 642.2 0 28 89 39 642.2 0 39 90 32 642.2 0 32 91 24 642.2 0 24 92 27 642.2 0 27 93 31 642.2 0 31 94 32 642.2 0 32 95 66 642.2 0 66 96 67 642.2 0 67 97 38 642.2 0 38 98 20 642.2 0 20 99 18 642.2 0 18 100 27 642.2 0 27 101 30 642.2 0 30 102 20 642.2 0 20 103 20 642.2 0 20 104 77 642.2 0 77 105 36 642.2 0 36 106 20 642.2 0 20 107 28 642.2 0 28 108 36 642.2 0 36 109 24 642.2 0 24 110 28 642.2 0 28 111 21 642.2 0 21 112 33 642.2 0 33 113 32 642.2 0 32 114 76 642.2 0 76 115 49 642.2 0 49 116 24 642.2 0 24 117 21 642.2 0 21 118 28 642.2 0 28 119 36 642.2 0 36 120 47 642.2 0 47 121 44 642.2 0 44 122 63 642.2 0 63 123 42 642.2 0 42 124 25 642.2 0 25 125 89 642.2 0 89 126 123 642.2 0 123 127 85 642.2 0 85 128 217 642.2 0 217 129 104 642.2 0 104 130 95 642.2 0 95 131 167 642.2 0 167 132 196 642.2 0 196 133 127 642.2 0 127 134 85 642.2 0 85 135 568 642.2 0 568 136 296 642.2 0 296 137 385 642.2 0 385 138 43 642.2 0 43 139 49 642.2 0 49 140 171 642.2 0 171 141 154 642.2 0 154 142 440 642.2 0 440 143 127 642.2 0 127 144 42 642.2 0 42 145 66 642.2 0 66 146 36 642.2 0 36 147 74 642.2 0 74 148 136 642.2 0 136 149 140 642.2 0 140 150 102 642.2 0 102 151 56 642.2 0 56 152 67 642.2 0 67 153 37 642.2 0 37 154 49 642.2 0 49 155 51 642.2 0 51 156 47 642.2 0 47 157 45 642.2 0 45 158 25 642.2 0 25 159 52 642.2 0 52 160 63 642.2 0 63 161 64 642.2 0 64 162 39 642.2 0 39 163 45 642.2 0 45 164 65 642.2 0 65 165 54 642.2 0 54 166 58 642.2 0 58 167 78 642.2 0 78 168 68 642.2 0 68 169 37 642.2 0 37 170 36 642.2 0 36 171 52 642.2 0 52 172 36 642.2 0 36 173 54 642.2 0 54 174 35 642.2 0 35 175 72 642.2 0 72 176 65 642.2 0 65 177 36 642.2 0 36 178 54 642.2 0 54 179 39 642.2 0 39 180 72 642.2 0 72 181 54 642.2 0 54 182 71 642.2 0 71 183 75 642.2 0 75 184 60 642.2 0 60 185 71 642.2 0 71 186 70 642.2 0 70 187 68 642.2 0 68 188 175 642.2 0 175 189 94 642.2 0 94 190 46 642.2 0 46 191 63 642.2 0 63 192 48 642.2 0 48 193 52 642.2 0 52 194 89 642.2 0 89 195 74 642.2 0 74 196 69 642.2 0 69 197 41 642.2 0 41 198 33 642.2 0 33 199 40 642.2 0 40 200 47 642.2 0 47 201 56 642.2 0 56 202 73 642.2 0 73 203 43 642.2 0 43 204 32 642.2 0 32 205 36 642.2 0 36 206 28 642.2 0 28 207 104 642.2 0 104 208 147 642.2 0 147 209 200 642.2 0 200 210 109 642.2 0 109 211 491 642.2 0 491 212 76 642.2 0 76 213 105 642.2 0 105 214 265 642.2 0 265 215 213 642.2 0 213 216 185 642.2 0 185 217 114 642.2 0 114 218 47 642.2 0 47 219 64 642.2 0 64 220 77 642.2 0 77 221 92 642.2 0 92 222 86 642.2 0 86 223 47 642.2 0 47 224 74 642.2 0 74 225 90 642.2 0 90 226 87 642.2 0 87 227 51 642.2 0 51 228 96 642.2 0 96 229 87 642.2 0 87 230 142 642.2 0 142 231 60 642.2 0 60 232 98 642.2 0 98 233 164 642.2 0 164 234 184 642.2 0 184 235 200 642.2 0 200 236 88 642.2 0 88 237 48 642.2 0 48 238 52 642.2 0 52 239 62 642.2 0 62 240 61 642.2 0 61 241 57 642.2 0 57 242 58 642.2 0 58 243 77 642.2 0 77 244 87 642.2 0 87 245 73 642.2 0 73 246 38 642.2 0 38 247 38 642.2 0 38 248 48 642.2 0 48 249 41 642.2 0 41 250 34 642.2 0 34 251 54 642.2 0 54 252 61 642.2 0 61 253 74 642.2 0 74 254 72 642.2 0 72 255 79 642.2 0 79 256 59 642.2 0 59 257 83 642.2 0 83 258 86 642.2 0 86 259 65 642.2 0 65 260 104 642.2 0 104 261 86 642.2 0 86 262 87 642.2 0 87 263 96 642.2 0 96 264 81 642.2 0 81 265 79 642.2 0 79 266 91 642.2 0 91 267 73 642.2 0 73 268 199 642.2 0 199 269 598 642.2 0 598 270 337 642.2 0 337 271 516 642.2 0 516 272 161 642.2 0 161 273 327 642.2 0 327 274 209 642.2 0 209 275 154 642.2 0 154 276 122 642.2 0 122 277 97 642.2 0 97 278 73 642.2 0 73 279 105 642.2 0 105 280 104 642.2 0 104 281 130 642.2 0 130 282 96 642.2 0 96 283 150 642.2 0 150 284 143 642.2 0 143 285 75 642.2 0 75 286 73 642.2 0 73 287 76 642.2 0 76 288 90 642.2 0 90 289 101 642.2 0 101 290 109 642.2 0 109 291 101 642.2 0 101 292 125 642.2 0 125 293 137 642.2 0 137 294 569 642.2 0 569 295 572 642.2 0 572 296 902 642.2 0 902 297 340 642.2 0 340 298 352 642.2 0 352 299 108 642.2 0 108 300 145 642.2 0 145 301 454 642.2 0 454 Finished in 3.79 s (49 us/read; 1.23 M reads/minute). === Summary === Total reads processed: 77,414 Reads with adapters: 34,432 (44.5%) Reads that were too short: 116 (0.1%) Reads written (passing filters): 34,316 (44.3%) Total basepairs processed: 21,378,302 bp Total written (filtered): 9,484,699 bp (44.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 34432 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 14 0.0 2 0 0 0 14 15 21 0.0 3 0 0 9 12 16 346 0.0 3 0 281 45 20 17 33513 0.0 3 30235 2631 510 137 18 73 0.0 3 5 54 7 7 19 110 0.0 3 0 0 43 67 20 99 0.0 3 0 0 0 99 21 2 0.0 3 0 0 0 2 32 2 0.0 3 0 0 0 2 39 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 43 8 0.0 3 0 0 0 8 45 1 0.0 3 0 0 0 1 46 2 0.0 3 0 0 0 2 47 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 51 2 0.0 3 0 0 0 2 52 7 0.0 3 0 0 0 7 53 1 0.0 3 0 0 0 1 54 2 0.0 3 0 0 0 2 55 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 64 2 0.0 3 0 0 0 2 65 13 0.0 3 0 0 1 12 66 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 1 69 2 0.0 3 0 0 0 2 73 1 0.0 3 0 0 0 1 74 3 0.0 3 0 0 0 3 76 1 0.0 3 0 0 0 1 77 2 0.0 3 0 0 0 2 79 7 0.0 3 0 0 0 7 80 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 85 2 0.0 3 0 0 0 2 86 4 0.0 3 0 0 0 4 87 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 1 97 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 105 2 0.0 3 0 0 0 2 106 2 0.0 3 0 0 0 2 107 1 0.0 3 0 0 0 1 110 2 0.0 3 0 0 0 2 116 2 0.0 3 0 0 0 2 117 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 125 3 0.0 3 0 0 0 3 127 3 0.0 3 0 0 0 3 128 12 0.0 3 0 0 0 12 130 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 135 8 0.0 3 0 0 0 8 136 3 0.0 3 0 0 0 3 137 1 0.0 3 0 0 0 1 138 5 0.0 3 0 0 0 5 143 2 0.0 3 0 1 0 1 146 2 0.0 3 0 0 0 2 147 3 0.0 3 0 0 0 3 148 5 0.0 3 0 0 0 5 149 1 0.0 3 0 0 1 150 2 0.0 3 0 0 0 2 151 3 0.0 3 0 0 0 3 152 5 0.0 3 0 0 0 5 153 1 0.0 3 0 0 0 1 155 4 0.0 3 0 0 0 4 156 2 0.0 3 0 0 0 2 158 1 0.0 3 0 0 0 1 161 2 0.0 3 0 0 0 2 162 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 164 2 0.0 3 0 0 0 2 165 2 0.0 3 0 0 0 2 168 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 172 2 0.0 3 0 0 0 2 173 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 183 2 0.0 3 0 0 0 2 184 3 0.0 3 0 0 0 3 185 4 0.0 3 0 0 0 4 187 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 1 190 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 205 3 0.0 3 0 0 0 3 206 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 217 15 0.0 3 0 0 1 14 221 4 0.0 3 0 0 0 4 222 2 0.0 3 0 0 0 2 227 1 0.0 3 0 0 0 1 234 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 239 2 0.0 3 0 0 0 2 240 2 0.0 3 0 0 0 2 241 1 0.0 3 0 0 0 1 243 1 0.0 3 0 0 0 1 247 1 0.0 3 0 0 0 1 249 1 0.0 3 0 0 0 1 250 2 0.0 3 0 0 1 1 262 1 0.0 3 0 0 0 1 271 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 280 3 0.0 3 0 0 1 2 281 5 0.0 3 0 0 0 5 282 4 0.0 3 0 0 0 4 283 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 290 1 0.0 3 0 0 0 1 291 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.20 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 34,316 Reads with adapters: 129 (0.4%) Reads written (passing filters): 34,316 (100.0%) Total basepairs processed: 9,484,699 bp Total written (filtered): 9,484,296 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 129 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 14.7% C: 58.9% G: 20.2% T: 6.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 123 536.2 0 123 4 1 134.0 0 1 6 5 8.4 0 5