This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACATTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 69.53 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 82,646 (3.1%) Reads that were too short: 6,924 (0.3%) Reads written (passing filters): 75,722 (2.9%) Total basepairs processed: 791,719,999 bp Total written (filtered): 21,325,500 bp (2.7%) === Adapter 1 === Sequence: ACATTG; Type: regular 5'; Length: 6; Trimmed: 82646 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 574 642.2 0 574 7 31762 642.2 0 31762 8 34642 642.2 0 34642 9 30 642.2 0 30 10 14 642.2 0 14 11 20 642.2 0 20 12 12 642.2 0 12 13 19 642.2 0 19 14 31 642.2 0 31 15 26 642.2 0 26 16 25 642.2 0 25 17 29 642.2 0 29 18 23 642.2 0 23 19 41 642.2 0 41 20 39 642.2 0 39 21 16 642.2 0 16 22 23 642.2 0 23 23 36 642.2 0 36 24 20 642.2 0 20 25 24 642.2 0 24 26 18 642.2 0 18 27 18 642.2 0 18 28 28 642.2 0 28 29 20 642.2 0 20 30 25 642.2 0 25 31 17 642.2 0 17 32 28 642.2 0 28 33 23 642.2 0 23 34 21 642.2 0 21 35 54 642.2 0 54 36 348 642.2 0 348 37 125 642.2 0 125 38 186 642.2 0 186 39 18 642.2 0 18 40 19 642.2 0 19 41 25 642.2 0 25 42 32 642.2 0 32 43 64 642.2 0 64 44 95 642.2 0 95 45 184 642.2 0 184 46 21 642.2 0 21 47 19 642.2 0 19 48 22 642.2 0 22 49 43 642.2 0 43 50 33 642.2 0 33 51 29 642.2 0 29 52 36 642.2 0 36 53 38 642.2 0 38 54 27 642.2 0 27 55 33 642.2 0 33 56 30 642.2 0 30 57 44 642.2 0 44 58 49 642.2 0 49 59 37 642.2 0 37 60 20 642.2 0 20 61 34 642.2 0 34 62 34 642.2 0 34 63 38 642.2 0 38 64 42 642.2 0 42 65 19 642.2 0 19 66 35 642.2 0 35 67 15 642.2 0 15 68 21 642.2 0 21 69 15 642.2 0 15 70 16 642.2 0 16 71 22 642.2 0 22 72 34 642.2 0 34 73 31 642.2 0 31 74 24 642.2 0 24 75 35 642.2 0 35 76 40 642.2 0 40 77 42 642.2 0 42 78 28 642.2 0 28 79 51 642.2 0 51 80 24 642.2 0 24 81 26 642.2 0 26 82 21 642.2 0 21 83 18 642.2 0 18 84 28 642.2 0 28 85 40 642.2 0 40 86 31 642.2 0 31 87 45 642.2 0 45 88 35 642.2 0 35 89 36 642.2 0 36 90 46 642.2 0 46 91 40 642.2 0 40 92 31 642.2 0 31 93 35 642.2 0 35 94 34 642.2 0 34 95 50 642.2 0 50 96 48 642.2 0 48 97 33 642.2 0 33 98 32 642.2 0 32 99 26 642.2 0 26 100 23 642.2 0 23 101 34 642.2 0 34 102 21 642.2 0 21 103 25 642.2 0 25 104 73 642.2 0 73 105 26 642.2 0 26 106 27 642.2 0 27 107 31 642.2 0 31 108 25 642.2 0 25 109 22 642.2 0 22 110 15 642.2 0 15 111 30 642.2 0 30 112 25 642.2 0 25 113 21 642.2 0 21 114 77 642.2 0 77 115 51 642.2 0 51 116 24 642.2 0 24 117 20 642.2 0 20 118 41 642.2 0 41 119 20 642.2 0 20 120 29 642.2 0 29 121 37 642.2 0 37 122 81 642.2 0 81 123 28 642.2 0 28 124 17 642.2 0 17 125 36 642.2 0 36 126 46 642.2 0 46 127 33 642.2 0 33 128 77 642.2 0 77 129 46 642.2 0 46 130 54 642.2 0 54 131 169 642.2 0 169 132 212 642.2 0 212 133 136 642.2 0 136 134 67 642.2 0 67 135 271 642.2 0 271 136 199 642.2 0 199 137 153 642.2 0 153 138 28 642.2 0 28 139 26 642.2 0 26 140 90 642.2 0 90 141 80 642.2 0 80 142 164 642.2 0 164 143 101 642.2 0 101 144 19 642.2 0 19 145 48 642.2 0 48 146 46 642.2 0 46 147 40 642.2 0 40 148 61 642.2 0 61 149 67 642.2 0 67 150 47 642.2 0 47 151 52 642.2 0 52 152 57 642.2 0 57 153 45 642.2 0 45 154 44 642.2 0 44 155 49 642.2 0 49 156 29 642.2 0 29 157 36 642.2 0 36 158 26 642.2 0 26 159 31 642.2 0 31 160 32 642.2 0 32 161 43 642.2 0 43 162 36 642.2 0 36 163 35 642.2 0 35 164 50 642.2 0 50 165 42 642.2 0 42 166 37 642.2 0 37 167 30 642.2 0 30 168 38 642.2 0 38 169 23 642.2 0 23 170 40 642.2 0 40 171 43 642.2 0 43 172 23 642.2 0 23 173 59 642.2 0 59 174 33 642.2 0 33 175 34 642.2 0 34 176 39 642.2 0 39 177 29 642.2 0 29 178 33 642.2 0 33 179 31 642.2 0 31 180 37 642.2 0 37 181 38 642.2 0 38 182 43 642.2 0 43 183 52 642.2 0 52 184 44 642.2 0 44 185 44 642.2 0 44 186 42 642.2 0 42 187 30 642.2 0 30 188 193 642.2 0 193 189 96 642.2 0 96 190 42 642.2 0 42 191 41 642.2 0 41 192 20 642.2 0 20 193 28 642.2 0 28 194 45 642.2 0 45 195 34 642.2 0 34 196 29 642.2 0 29 197 18 642.2 0 18 198 21 642.2 0 21 199 34 642.2 0 34 200 24 642.2 0 24 201 39 642.2 0 39 202 37 642.2 0 37 203 30 642.2 0 30 204 34 642.2 0 34 205 29 642.2 0 29 206 18 642.2 0 18 207 45 642.2 0 45 208 74 642.2 0 74 209 95 642.2 0 95 210 38 642.2 0 38 211 486 642.2 0 486 212 42 642.2 0 42 213 35 642.2 0 35 214 91 642.2 0 91 215 81 642.2 0 81 216 68 642.2 0 68 217 39 642.2 0 39 218 20 642.2 0 20 219 43 642.2 0 43 220 30 642.2 0 30 221 44 642.2 0 44 222 52 642.2 0 52 223 39 642.2 0 39 224 29 642.2 0 29 225 51 642.2 0 51 226 32 642.2 0 32 227 41 642.2 0 41 228 41 642.2 0 41 229 23 642.2 0 23 230 57 642.2 0 57 231 25 642.2 0 25 232 36 642.2 0 36 233 53 642.2 0 53 234 56 642.2 0 56 235 72 642.2 0 72 236 51 642.2 0 51 237 39 642.2 0 39 238 50 642.2 0 50 239 31 642.2 0 31 240 43 642.2 0 43 241 40 642.2 0 40 242 49 642.2 0 49 243 49 642.2 0 49 244 80 642.2 0 80 245 50 642.2 0 50 246 31 642.2 0 31 247 32 642.2 0 32 248 26 642.2 0 26 249 39 642.2 0 39 250 41 642.2 0 41 251 25 642.2 0 25 252 38 642.2 0 38 253 40 642.2 0 40 254 41 642.2 0 41 255 41 642.2 0 41 256 52 642.2 0 52 257 57 642.2 0 57 258 55 642.2 0 55 259 39 642.2 0 39 260 39 642.2 0 39 261 37 642.2 0 37 262 47 642.2 0 47 263 34 642.2 0 34 264 45 642.2 0 45 265 51 642.2 0 51 266 73 642.2 0 73 267 41 642.2 0 41 268 110 642.2 0 110 269 202 642.2 0 202 270 122 642.2 0 122 271 229 642.2 0 229 272 53 642.2 0 53 273 78 642.2 0 78 274 77 642.2 0 77 275 44 642.2 0 44 276 63 642.2 0 63 277 49 642.2 0 49 278 42 642.2 0 42 279 107 642.2 0 107 280 62 642.2 0 62 281 79 642.2 0 79 282 58 642.2 0 58 283 58 642.2 0 58 284 95 642.2 0 95 285 53 642.2 0 53 286 51 642.2 0 51 287 58 642.2 0 58 288 51 642.2 0 51 289 51 642.2 0 51 290 56 642.2 0 56 291 61 642.2 0 61 292 45 642.2 0 45 293 74 642.2 0 74 294 193 642.2 0 193 295 342 642.2 0 342 296 289 642.2 0 289 297 192 642.2 0 192 298 146 642.2 0 146 299 43 642.2 0 43 300 78 642.2 0 78 301 221 642.2 0 221 Finished in 4.06 s (54 us/read; 1.12 M reads/minute). === Summary === Total reads processed: 75,722 Reads with adapters: 35,898 (47.4%) Reads that were too short: 18 (0.0%) Reads written (passing filters): 35,880 (47.4%) Total basepairs processed: 21,325,500 bp Total written (filtered): 9,794,675 bp (45.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 35898 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 2 1 18 11 0.0 3 0 0 11 19 437 0.0 3 0 430 7 20 35328 0.0 4 34749 552 24 3 21 57 0.0 4 10 47 22 6 0.0 4 0 0 5 0 1 23 21 0.0 4 0 0 0 19 2 24 1 0.0 4 1 26 1 0.0 4 0 0 1 34 1 0.0 4 0 0 0 0 1 43 1 0.0 4 1 88 1 0.0 4 1 91 4 0.0 4 0 0 0 0 4 98 2 0.0 4 0 0 0 0 2 125 1 0.0 4 1 127 4 0.0 4 0 0 0 0 4 134 1 0.0 4 0 0 0 0 1 135 3 0.0 4 0 0 0 0 3 153 1 0.0 4 0 0 0 0 1 168 1 0.0 4 0 0 0 0 1 202 1 0.0 4 0 0 0 0 1 228 3 0.0 4 0 0 0 0 3 229 1 0.0 4 0 0 0 0 1 231 1 0.0 4 0 0 0 0 1 232 1 0.0 4 0 0 0 0 1 241 1 0.0 4 0 0 0 0 1 259 1 0.0 4 0 0 0 1 264 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 293 1 0.0 4 0 0 0 0 1 294 1 0.0 4 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.26 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 35,880 Reads with adapters: 3,748 (10.4%) Reads written (passing filters): 35,880 (100.0%) Total basepairs processed: 9,794,675 bp Total written (filtered): 9,753,362 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3748 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.0% C: 3.4% G: 93.8% T: 1.8% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 20 560.6 0 20 4 1 140.2 0 1 5 3 35.0 0 3 6 1 8.8 0 1 7 1 2.2 0 1 8 1 0.5 0 1 9 8 0.1 0 0 8 10 210 0.0 1 11 199 11 3489 0.0 1 23 3466 12 9 0.0 1 0 9 14 2 0.0 1 0 2 151 2 0.0 1 0 2 224 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.