This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGCGAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 69.42 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 91,154 (3.5%) Reads that were too short: 11,749 (0.4%) Reads written (passing filters): 79,405 (3.0%) Total basepairs processed: 791,719,999 bp Total written (filtered): 17,157,018 bp (2.2%) === Adapter 1 === Sequence: CGCGAT; Type: regular 5'; Length: 6; Trimmed: 91154 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 860 642.2 0 860 7 12411 642.2 0 12411 8 222 642.2 0 222 9 11551 642.2 0 11551 10 44 642.2 0 44 11 55 642.2 0 55 12 34 642.2 0 34 13 51 642.2 0 51 14 49 642.2 0 49 15 65 642.2 0 65 16 39 642.2 0 39 17 48 642.2 0 48 18 43 642.2 0 43 19 51 642.2 0 51 20 36 642.2 0 36 21 60 642.2 0 60 22 65 642.2 0 65 23 54 642.2 0 54 24 57 642.2 0 57 25 38 642.2 0 38 26 70 642.2 0 70 27 39 642.2 0 39 28 51 642.2 0 51 29 45 642.2 0 45 30 54 642.2 0 54 31 64 642.2 0 64 32 30 642.2 0 30 33 31 642.2 0 31 34 47 642.2 0 47 35 48 642.2 0 48 36 28 642.2 0 28 37 98 642.2 0 98 38 50 642.2 0 50 39 48 642.2 0 48 40 53 642.2 0 53 41 48 642.2 0 48 42 159 642.2 0 159 43 119 642.2 0 119 44 248 642.2 0 248 45 39 642.2 0 39 46 42 642.2 0 42 47 40 642.2 0 40 48 39 642.2 0 39 49 46 642.2 0 46 50 49 642.2 0 49 51 36 642.2 0 36 52 42 642.2 0 42 53 39 642.2 0 39 54 39 642.2 0 39 55 55 642.2 0 55 56 42 642.2 0 42 57 37 642.2 0 37 58 71 642.2 0 71 59 50 642.2 0 50 60 39 642.2 0 39 61 44 642.2 0 44 62 46 642.2 0 46 63 47 642.2 0 47 64 33 642.2 0 33 65 54 642.2 0 54 66 59 642.2 0 59 67 51 642.2 0 51 68 53 642.2 0 53 69 51 642.2 0 51 70 39 642.2 0 39 71 50 642.2 0 50 72 49 642.2 0 49 73 60 642.2 0 60 74 51 642.2 0 51 75 68 642.2 0 68 76 65 642.2 0 65 77 58 642.2 0 58 78 36 642.2 0 36 79 54 642.2 0 54 80 81 642.2 0 81 81 62 642.2 0 62 82 34 642.2 0 34 83 45 642.2 0 45 84 56 642.2 0 56 85 42 642.2 0 42 86 41 642.2 0 41 87 45 642.2 0 45 88 53 642.2 0 53 89 69 642.2 0 69 90 65 642.2 0 65 91 101 642.2 0 101 92 605 642.2 0 605 93 395 642.2 0 395 94 636 642.2 0 636 95 60 642.2 0 60 96 80 642.2 0 80 97 70 642.2 0 70 98 60 642.2 0 60 99 71 642.2 0 71 100 70 642.2 0 70 101 50 642.2 0 50 102 45 642.2 0 45 103 37 642.2 0 37 104 64 642.2 0 64 105 62 642.2 0 62 106 47 642.2 0 47 107 40 642.2 0 40 108 79 642.2 0 79 109 85 642.2 0 85 110 84 642.2 0 84 111 74 642.2 0 74 112 72 642.2 0 72 113 78 642.2 0 78 114 61 642.2 0 61 115 97 642.2 0 97 116 382 642.2 0 382 117 145 642.2 0 145 118 92 642.2 0 92 119 129 642.2 0 129 120 256 642.2 0 256 121 408 642.2 0 408 122 984 642.2 0 984 123 36605 642.2 0 36605 124 559 642.2 0 559 125 899 642.2 0 899 126 59 642.2 0 59 127 60 642.2 0 60 128 84 642.2 0 84 129 115 642.2 0 115 130 447 642.2 0 447 131 155 642.2 0 155 132 420 642.2 0 420 133 59 642.2 0 59 134 48 642.2 0 48 135 41 642.2 0 41 136 49 642.2 0 49 137 49 642.2 0 49 138 47 642.2 0 47 139 46 642.2 0 46 140 59 642.2 0 59 141 38 642.2 0 38 142 54 642.2 0 54 143 49 642.2 0 49 144 43 642.2 0 43 145 60 642.2 0 60 146 73 642.2 0 73 147 51 642.2 0 51 148 40 642.2 0 40 149 41 642.2 0 41 150 31 642.2 0 31 151 52 642.2 0 52 152 62 642.2 0 62 153 68 642.2 0 68 154 67 642.2 0 67 155 55 642.2 0 55 156 45 642.2 0 45 157 40 642.2 0 40 158 37 642.2 0 37 159 39 642.2 0 39 160 58 642.2 0 58 161 48 642.2 0 48 162 45 642.2 0 45 163 53 642.2 0 53 164 55 642.2 0 55 165 58 642.2 0 58 166 46 642.2 0 46 167 71 642.2 0 71 168 67 642.2 0 67 169 54 642.2 0 54 170 46 642.2 0 46 171 47 642.2 0 47 172 48 642.2 0 48 173 54 642.2 0 54 174 71 642.2 0 71 175 208 642.2 0 208 176 191 642.2 0 191 177 94 642.2 0 94 178 165 642.2 0 165 179 133 642.2 0 133 180 224 642.2 0 224 181 93 642.2 0 93 182 61 642.2 0 61 183 81 642.2 0 81 184 270 642.2 0 270 185 58 642.2 0 58 186 49 642.2 0 49 187 52 642.2 0 52 188 65 642.2 0 65 189 53 642.2 0 53 190 49 642.2 0 49 191 57 642.2 0 57 192 55 642.2 0 55 193 54 642.2 0 54 194 59 642.2 0 59 195 57 642.2 0 57 196 61 642.2 0 61 197 63 642.2 0 63 198 105 642.2 0 105 199 212 642.2 0 212 200 166 642.2 0 166 201 155 642.2 0 155 202 126 642.2 0 126 203 51 642.2 0 51 204 62 642.2 0 62 205 85 642.2 0 85 206 63 642.2 0 63 207 82 642.2 0 82 208 93 642.2 0 93 209 66 642.2 0 66 210 60 642.2 0 60 211 63 642.2 0 63 212 53 642.2 0 53 213 43 642.2 0 43 214 59 642.2 0 59 215 70 642.2 0 70 216 54 642.2 0 54 217 34 642.2 0 34 218 68 642.2 0 68 219 108 642.2 0 108 220 134 642.2 0 134 221 165 642.2 0 165 222 1169 642.2 0 1169 223 513 642.2 0 513 224 199 642.2 0 199 225 336 642.2 0 336 226 73 642.2 0 73 227 71 642.2 0 71 228 72 642.2 0 72 229 64 642.2 0 64 230 64 642.2 0 64 231 58 642.2 0 58 232 58 642.2 0 58 233 67 642.2 0 67 234 54 642.2 0 54 235 62 642.2 0 62 236 53 642.2 0 53 237 54 642.2 0 54 238 108 642.2 0 108 239 156 642.2 0 156 240 991 642.2 0 991 241 660 642.2 0 660 242 712 642.2 0 712 243 64 642.2 0 64 244 52 642.2 0 52 245 61 642.2 0 61 246 61 642.2 0 61 247 76 642.2 0 76 248 77 642.2 0 77 249 69 642.2 0 69 250 87 642.2 0 87 251 116 642.2 0 116 252 112 642.2 0 112 253 82 642.2 0 82 254 62 642.2 0 62 255 52 642.2 0 52 256 66 642.2 0 66 257 77 642.2 0 77 258 70 642.2 0 70 259 52 642.2 0 52 260 63 642.2 0 63 261 67 642.2 0 67 262 117 642.2 0 117 263 82 642.2 0 82 264 54 642.2 0 54 265 54 642.2 0 54 266 67 642.2 0 67 267 66 642.2 0 66 268 85 642.2 0 85 269 70 642.2 0 70 270 50 642.2 0 50 271 41 642.2 0 41 272 65 642.2 0 65 273 62 642.2 0 62 274 73 642.2 0 73 275 63 642.2 0 63 276 65 642.2 0 65 277 70 642.2 0 70 278 54 642.2 0 54 279 79 642.2 0 79 280 53 642.2 0 53 281 57 642.2 0 57 282 62 642.2 0 62 283 50 642.2 0 50 284 58 642.2 0 58 285 52 642.2 0 52 286 74 642.2 0 74 287 103 642.2 0 103 288 185 642.2 0 185 289 146 642.2 0 146 290 286 642.2 0 286 291 136 642.2 0 136 292 118 642.2 0 118 293 77 642.2 0 77 294 68 642.2 0 68 295 66 642.2 0 66 296 64 642.2 0 64 297 80 642.2 0 80 298 63 642.2 0 63 299 59 642.2 0 59 300 83 642.2 0 83 301 93 642.2 0 93 Finished in 3.67 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 79,405 Reads with adapters: 13,063 (16.5%) Reads that were too short: 30 (0.0%) Reads written (passing filters): 13,033 (16.4%) Total basepairs processed: 17,157,018 bp Total written (filtered): 3,570,797 bp (20.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 13063 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 5 0.0 3 0 0 5 19 158 0.0 3 0 153 4 1 20 12831 0.0 4 12641 187 3 21 20 0.0 4 1 19 22 2 0.0 4 1 0 1 23 8 0.0 4 0 0 0 7 1 31 1 0.0 4 0 0 0 0 1 42 2 0.0 4 0 0 0 0 2 47 1 0.0 4 0 0 0 0 1 62 1 0.0 4 0 0 0 0 1 86 3 0.0 4 0 0 0 0 3 91 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 122 1 0.0 4 1 126 1 0.0 4 0 1 127 2 0.0 4 0 0 0 0 2 168 1 0.0 4 0 0 0 0 1 194 12 0.0 4 0 0 0 0 12 227 1 0.0 4 0 0 0 0 1 228 7 0.0 4 0 0 0 0 7 229 1 0.0 4 0 0 0 0 1 232 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.44 s (34 us/read; 1.78 M reads/minute). === Summary === Total reads processed: 13,033 Reads with adapters: 377 (2.9%) Reads written (passing filters): 13,033 (100.0%) Total basepairs processed: 3,570,797 bp Total written (filtered): 3,563,154 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 377 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 10.1% C: 3.7% G: 79.6% T: 6.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 44 203.6 0 44 4 2 50.9 0 2 5 1 12.7 0 1 10 12 0.0 1 0 12 11 272 0.0 1 3 269 12 28 0.0 1 1 27 225 18 0.0 1 17 1