This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCTGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 71.99 s (27 us/read; 2.19 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 274,093 (10.4%) Reads that were too short: 60,140 (2.3%) Reads written (passing filters): 213,953 (8.1%) Total basepairs processed: 791,719,999 bp Total written (filtered): 55,700,247 bp (7.0%) === Adapter 1 === Sequence: AGCTGG; Type: regular 5'; Length: 6; Trimmed: 274093 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1509 642.2 0 1509 7 22880 642.2 0 22880 8 1529 642.2 0 1529 9 17430 642.2 0 17430 10 3715 642.2 0 3715 11 101048 642.2 0 101048 12 229 642.2 0 229 13 109 642.2 0 109 14 117 642.2 0 117 15 117 642.2 0 117 16 88 642.2 0 88 17 97 642.2 0 97 18 112 642.2 0 112 19 108 642.2 0 108 20 105 642.2 0 105 21 71 642.2 0 71 22 103 642.2 0 103 23 118 642.2 0 118 24 120 642.2 0 120 25 84 642.2 0 84 26 151 642.2 0 151 27 210 642.2 0 210 28 200 642.2 0 200 29 619 642.2 0 619 30 111 642.2 0 111 31 111 642.2 0 111 32 142 642.2 0 142 33 125 642.2 0 125 34 129 642.2 0 129 35 101 642.2 0 101 36 102 642.2 0 102 37 128 642.2 0 128 38 110 642.2 0 110 39 116 642.2 0 116 40 334 642.2 0 334 41 281 642.2 0 281 42 119 642.2 0 119 43 122 642.2 0 122 44 123 642.2 0 123 45 244 642.2 0 244 46 100 642.2 0 100 47 132 642.2 0 132 48 224 642.2 0 224 49 443 642.2 0 443 50 107 642.2 0 107 51 124 642.2 0 124 52 109 642.2 0 109 53 136 642.2 0 136 54 130 642.2 0 130 55 182 642.2 0 182 56 161 642.2 0 161 57 147 642.2 0 147 58 137 642.2 0 137 59 259 642.2 0 259 60 172 642.2 0 172 61 211 642.2 0 211 62 159 642.2 0 159 63 96 642.2 0 96 64 117 642.2 0 117 65 112 642.2 0 112 66 99 642.2 0 99 67 294 642.2 0 294 68 146 642.2 0 146 69 180 642.2 0 180 70 185 642.2 0 185 71 751 642.2 0 751 72 135 642.2 0 135 73 116 642.2 0 116 74 93 642.2 0 93 75 111 642.2 0 111 76 122 642.2 0 122 77 109 642.2 0 109 78 227 642.2 0 227 79 683 642.2 0 683 80 718 642.2 0 718 81 316 642.2 0 316 82 593 642.2 0 593 83 472 642.2 0 472 84 765 642.2 0 765 85 699 642.2 0 699 86 119 642.2 0 119 87 76 642.2 0 76 88 88 642.2 0 88 89 162 642.2 0 162 90 162 642.2 0 162 91 148 642.2 0 148 92 89 642.2 0 89 93 115 642.2 0 115 94 104 642.2 0 104 95 130 642.2 0 130 96 136 642.2 0 136 97 222 642.2 0 222 98 1864 642.2 0 1864 99 231 642.2 0 231 100 221 642.2 0 221 101 488 642.2 0 488 102 122 642.2 0 122 103 105 642.2 0 105 104 148 642.2 0 148 105 153 642.2 0 153 106 161 642.2 0 161 107 316 642.2 0 316 108 183 642.2 0 183 109 301 642.2 0 301 110 314 642.2 0 314 111 376 642.2 0 376 112 1528 642.2 0 1528 113 7272 642.2 0 7272 114 3398 642.2 0 3398 115 15310 642.2 0 15310 116 164 642.2 0 164 117 126 642.2 0 126 118 101 642.2 0 101 119 195 642.2 0 195 120 113 642.2 0 113 121 106 642.2 0 106 122 119 642.2 0 119 123 135 642.2 0 135 124 234 642.2 0 234 125 185 642.2 0 185 126 166 642.2 0 166 127 137 642.2 0 137 128 286 642.2 0 286 129 280 642.2 0 280 130 583 642.2 0 583 131 460 642.2 0 460 132 107 642.2 0 107 133 118 642.2 0 118 134 732 642.2 0 732 135 129 642.2 0 129 136 225 642.2 0 225 137 144 642.2 0 144 138 237 642.2 0 237 139 280 642.2 0 280 140 182 642.2 0 182 141 318 642.2 0 318 142 322 642.2 0 322 143 200 642.2 0 200 144 485 642.2 0 485 145 157 642.2 0 157 146 118 642.2 0 118 147 135 642.2 0 135 148 105 642.2 0 105 149 139 642.2 0 139 150 145 642.2 0 145 151 93 642.2 0 93 152 94 642.2 0 94 153 138 642.2 0 138 154 363 642.2 0 363 155 656 642.2 0 656 156 1089 642.2 0 1089 157 441 642.2 0 441 158 135 642.2 0 135 159 99 642.2 0 99 160 113 642.2 0 113 161 105 642.2 0 105 162 98 642.2 0 98 163 132 642.2 0 132 164 199 642.2 0 199 165 108 642.2 0 108 166 137 642.2 0 137 167 390 642.2 0 390 168 295 642.2 0 295 169 127 642.2 0 127 170 84 642.2 0 84 171 236 642.2 0 236 172 175 642.2 0 175 173 80 642.2 0 80 174 93 642.2 0 93 175 109 642.2 0 109 176 87 642.2 0 87 177 106 642.2 0 106 178 203 642.2 0 203 179 87 642.2 0 87 180 135 642.2 0 135 181 60 642.2 0 60 182 79 642.2 0 79 183 85 642.2 0 85 184 149 642.2 0 149 185 70 642.2 0 70 186 120 642.2 0 120 187 117 642.2 0 117 188 105 642.2 0 105 189 248 642.2 0 248 190 124 642.2 0 124 191 102 642.2 0 102 192 106 642.2 0 106 193 116 642.2 0 116 194 111 642.2 0 111 195 103 642.2 0 103 196 114 642.2 0 114 197 178 642.2 0 178 198 89 642.2 0 89 199 103 642.2 0 103 200 204 642.2 0 204 201 314 642.2 0 314 202 408 642.2 0 408 203 129 642.2 0 129 204 120 642.2 0 120 205 99 642.2 0 99 206 192 642.2 0 192 207 89 642.2 0 89 208 134 642.2 0 134 209 134 642.2 0 134 210 178 642.2 0 178 211 351 642.2 0 351 212 420 642.2 0 420 213 574 642.2 0 574 214 139 642.2 0 139 215 134 642.2 0 134 216 160 642.2 0 160 217 112 642.2 0 112 218 92 642.2 0 92 219 72 642.2 0 72 220 102 642.2 0 102 221 130 642.2 0 130 222 120 642.2 0 120 223 113 642.2 0 113 224 318 642.2 0 318 225 122 642.2 0 122 226 163 642.2 0 163 227 162 642.2 0 162 228 784 642.2 0 784 229 516 642.2 0 516 230 1014 642.2 0 1014 231 272 642.2 0 272 232 400 642.2 0 400 233 147 642.2 0 147 234 240 642.2 0 240 235 351 642.2 0 351 236 265 642.2 0 265 237 289 642.2 0 289 238 187 642.2 0 187 239 110 642.2 0 110 240 123 642.2 0 123 241 92 642.2 0 92 242 135 642.2 0 135 243 130 642.2 0 130 244 88 642.2 0 88 245 110 642.2 0 110 246 173 642.2 0 173 247 266 642.2 0 266 248 231 642.2 0 231 249 257 642.2 0 257 250 143 642.2 0 143 251 109 642.2 0 109 252 123 642.2 0 123 253 163 642.2 0 163 254 189 642.2 0 189 255 249 642.2 0 249 256 422 642.2 0 422 257 291 642.2 0 291 258 279 642.2 0 279 259 198 642.2 0 198 260 89 642.2 0 89 261 122 642.2 0 122 262 87 642.2 0 87 263 114 642.2 0 114 264 104 642.2 0 104 265 109 642.2 0 109 266 110 642.2 0 110 267 82 642.2 0 82 268 251 642.2 0 251 269 439 642.2 0 439 270 671 642.2 0 671 271 292 642.2 0 292 272 145 642.2 0 145 273 164 642.2 0 164 274 211 642.2 0 211 275 185 642.2 0 185 276 125 642.2 0 125 277 137 642.2 0 137 278 148 642.2 0 148 279 147 642.2 0 147 280 401 642.2 0 401 281 224 642.2 0 224 282 184 642.2 0 184 283 204 642.2 0 204 284 153 642.2 0 153 285 184 642.2 0 184 286 164 642.2 0 164 287 156 642.2 0 156 288 242 642.2 0 242 289 181 642.2 0 181 290 228 642.2 0 228 291 243 642.2 0 243 292 239 642.2 0 239 293 322 642.2 0 322 294 789 642.2 0 789 295 1826 642.2 0 1826 296 4232 642.2 0 4232 297 3415 642.2 0 3415 298 19628 642.2 0 19628 299 3426 642.2 0 3426 300 6550 642.2 0 6550 301 304 642.2 0 304 Finished in 9.21 s (43 us/read; 1.39 M reads/minute). === Summary === Total reads processed: 213,953 Reads with adapters: 22,489 (10.5%) Reads that were too short: 365 (0.2%) Reads written (passing filters): 22,124 (10.3%) Total basepairs processed: 55,700,247 bp Total written (filtered): 6,058,936 bp (10.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 22489 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 6 0.0 2 0 0 0 6 15 7 0.0 3 0 0 4 3 16 172 0.0 3 0 140 21 11 17 21211 0.0 3 19394 1484 253 80 18 67 0.0 3 9 37 7 14 19 64 0.0 3 0 0 25 39 20 80 0.0 3 1 0 1 78 21 2 0.0 3 0 0 0 2 22 1 0.0 3 0 0 0 1 23 31 0.0 3 1 0 2 28 24 2 0.0 3 0 0 1 1 25 2 0.0 3 0 0 1 1 26 1 0.0 3 0 0 0 1 27 1 0.0 3 1 28 2 0.0 3 0 0 1 1 32 85 0.0 3 0 0 1 84 34 3 0.0 3 0 0 0 3 35 18 0.0 3 0 0 0 18 37 2 0.0 3 0 0 1 1 38 2 0.0 3 0 0 0 2 39 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 46 3 0.0 3 0 0 0 3 47 2 0.0 3 0 0 0 2 48 2 0.0 3 0 0 0 2 50 17 0.0 3 0 0 0 17 51 2 0.0 3 0 0 0 2 52 2 0.0 3 0 0 0 2 53 46 0.0 3 0 0 0 46 55 1 0.0 3 0 0 0 1 56 13 0.0 3 0 0 1 12 57 1 0.0 3 0 0 0 1 58 10 0.0 3 0 0 0 10 60 1 0.0 3 0 0 1 61 6 0.0 3 0 0 0 6 62 5 0.0 3 0 0 0 5 63 9 0.0 3 0 0 0 9 64 4 0.0 3 0 0 0 4 65 1 0.0 3 0 0 0 1 67 3 0.0 3 0 0 0 3 68 1 0.0 3 0 0 0 1 72 2 0.0 3 0 0 0 2 73 115 0.0 3 0 0 101 14 76 6 0.0 3 0 0 1 5 77 33 0.0 3 0 0 2 31 78 3 0.0 3 0 0 1 2 79 6 0.0 3 0 0 0 6 80 1 0.0 3 0 0 0 1 81 4 0.0 3 0 0 0 4 83 1 0.0 3 0 0 0 1 84 2 0.0 3 0 0 0 2 85 4 0.0 3 0 0 0 4 86 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 1 88 1 0.0 3 0 0 0 1 90 2 0.0 3 0 0 0 2 91 1 0.0 3 0 0 0 1 92 2 0.0 3 0 0 0 2 94 4 0.0 3 0 0 0 4 96 2 0.0 3 0 0 0 2 98 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 103 2 0.0 3 0 0 0 2 104 11 0.0 3 0 0 0 11 105 1 0.0 3 0 0 0 1 107 3 0.0 3 0 0 0 3 108 1 0.0 3 0 0 0 1 109 2 0.0 3 0 0 0 2 110 1 0.0 3 0 0 0 1 112 2 0.0 3 0 0 0 2 113 2 0.0 3 0 0 1 1 114 2 0.0 3 0 0 0 2 115 2 0.0 3 0 0 1 1 116 2 0.0 3 0 0 0 2 120 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 122 12 0.0 3 0 0 0 12 123 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 125 30 0.0 3 0 0 1 29 126 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 1 129 2 0.0 3 0 0 1 1 131 1 0.0 3 0 0 0 1 133 3 0.0 3 0 0 0 3 134 1 0.0 3 0 0 0 1 135 2 0.0 3 0 0 0 2 136 6 0.0 3 0 0 0 6 139 3 0.0 3 0 0 0 3 141 1 0.0 3 0 0 0 1 143 4 0.0 3 0 0 0 4 144 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 153 15 0.0 3 0 0 0 15 154 26 0.0 3 0 0 1 25 155 1 0.0 3 0 0 0 1 156 2 0.0 3 0 0 0 2 158 2 0.0 3 0 0 0 2 160 3 0.0 3 1 0 0 2 162 2 0.0 3 0 0 0 2 163 3 0.0 3 0 0 0 3 164 6 0.0 3 0 0 0 6 165 4 0.0 3 0 0 0 4 166 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 168 4 0.0 3 0 0 1 3 170 8 0.0 3 0 0 0 8 171 1 0.0 3 0 0 0 1 172 2 0.0 3 0 0 0 2 173 1 0.0 3 0 0 0 1 175 5 0.0 3 0 0 0 5 176 7 0.0 3 0 0 1 6 177 2 0.0 3 0 0 1 1 178 9 0.0 3 0 0 0 9 179 2 0.0 3 0 0 0 2 180 6 0.0 3 0 0 0 6 182 4 0.0 3 0 0 0 4 183 1 0.0 3 0 0 0 1 185 8 0.0 3 0 0 0 8 186 2 0.0 3 0 0 0 2 188 2 0.0 3 0 0 0 2 189 3 0.0 3 0 0 1 2 190 8 0.0 3 0 0 0 8 191 2 0.0 3 0 0 0 2 192 5 0.0 3 1 0 0 4 193 1 0.0 3 0 0 0 1 194 1 0.0 3 0 1 195 3 0.0 3 1 0 0 2 199 4 0.0 3 0 1 0 3 200 35 0.0 3 0 0 0 35 202 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 204 1 0.0 3 0 0 0 1 205 2 0.0 3 0 0 0 2 210 2 0.0 3 0 0 0 2 211 1 0.0 3 0 0 0 1 213 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 215 1 0.0 3 0 0 0 1 218 1 0.0 3 0 0 0 1 219 4 0.0 3 0 0 0 4 220 1 0.0 3 0 0 0 1 221 7 0.0 3 0 0 0 7 223 2 0.0 3 0 0 0 2 224 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 229 2 0.0 3 0 0 1 1 230 1 0.0 3 0 0 0 1 234 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 1 236 24 0.0 3 0 0 1 23 237 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 239 1 0.0 3 0 0 0 1 241 1 0.0 3 0 0 0 1 242 1 0.0 3 0 0 0 1 243 1 0.0 3 0 0 0 1 244 5 0.0 3 0 0 0 5 253 1 0.0 3 0 0 0 1 257 1 0.0 3 0 0 0 1 259 1 0.0 3 0 0 0 1 260 1 0.0 3 0 0 0 1 261 1 0.0 3 0 0 0 1 263 1 0.0 3 0 0 0 1 264 1 0.0 3 0 0 0 1 268 1 0.0 3 0 0 0 1 269 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 276 1 0.0 3 0 0 0 1 278 6 0.0 3 0 0 0 6 279 2 0.0 3 0 0 0 2 280 5 0.0 3 0 0 0 5 281 4 0.0 3 0 0 1 3 282 1 0.0 3 0 0 0 1 283 2 0.0 3 0 0 0 2 287 3 0.0 3 0 0 1 2 288 1 0.0 3 0 0 1 290 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.77 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 22,124 Reads with adapters: 51 (0.2%) Reads written (passing filters): 22,124 (100.0%) Total basepairs processed: 6,058,936 bp Total written (filtered): 6,058,770 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 51 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 25.5% C: 41.2% G: 23.5% T: 9.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 42 345.7 0 42 4 6 86.4 0 6 5 2 21.6 0 2 6 1 5.4 0 1