This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCTGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 72.25 s (27 us/read; 2.18 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 295,805 (11.2%) Reads that were too short: 60,315 (2.3%) Reads written (passing filters): 235,490 (9.0%) Total basepairs processed: 791,719,999 bp Total written (filtered): 61,766,190 bp (7.8%) === Adapter 1 === Sequence: AGCTGG; Type: regular 5'; Length: 6; Trimmed: 295805 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1644 642.2 0 1644 7 19889 642.2 0 19889 8 1394 642.2 0 1394 9 22237 642.2 0 22237 10 3102 642.2 0 3102 11 120457 642.2 0 120457 12 261 642.2 0 261 13 99 642.2 0 99 14 116 642.2 0 116 15 114 642.2 0 114 16 91 642.2 0 91 17 74 642.2 0 74 18 78 642.2 0 78 19 82 642.2 0 82 20 91 642.2 0 91 21 90 642.2 0 90 22 79 642.2 0 79 23 105 642.2 0 105 24 93 642.2 0 93 25 84 642.2 0 84 26 107 642.2 0 107 27 114 642.2 0 114 28 98 642.2 0 98 29 655 642.2 0 655 30 110 642.2 0 110 31 79 642.2 0 79 32 119 642.2 0 119 33 107 642.2 0 107 34 113 642.2 0 113 35 112 642.2 0 112 36 134 642.2 0 134 37 111 642.2 0 111 38 110 642.2 0 110 39 131 642.2 0 131 40 494 642.2 0 494 41 342 642.2 0 342 42 104 642.2 0 104 43 96 642.2 0 96 44 137 642.2 0 137 45 322 642.2 0 322 46 93 642.2 0 93 47 116 642.2 0 116 48 246 642.2 0 246 49 463 642.2 0 463 50 128 642.2 0 128 51 101 642.2 0 101 52 115 642.2 0 115 53 112 642.2 0 112 54 105 642.2 0 105 55 181 642.2 0 181 56 132 642.2 0 132 57 123 642.2 0 123 58 125 642.2 0 125 59 298 642.2 0 298 60 176 642.2 0 176 61 231 642.2 0 231 62 177 642.2 0 177 63 128 642.2 0 128 64 147 642.2 0 147 65 147 642.2 0 147 66 101 642.2 0 101 67 301 642.2 0 301 68 137 642.2 0 137 69 159 642.2 0 159 70 162 642.2 0 162 71 713 642.2 0 713 72 155 642.2 0 155 73 123 642.2 0 123 74 106 642.2 0 106 75 101 642.2 0 101 76 111 642.2 0 111 77 121 642.2 0 121 78 144 642.2 0 144 79 334 642.2 0 334 80 496 642.2 0 496 81 235 642.2 0 235 82 683 642.2 0 683 83 566 642.2 0 566 84 824 642.2 0 824 85 684 642.2 0 684 86 95 642.2 0 95 87 101 642.2 0 101 88 101 642.2 0 101 89 163 642.2 0 163 90 149 642.2 0 149 91 156 642.2 0 156 92 98 642.2 0 98 93 125 642.2 0 125 94 105 642.2 0 105 95 142 642.2 0 142 96 124 642.2 0 124 97 196 642.2 0 196 98 1823 642.2 0 1823 99 248 642.2 0 248 100 280 642.2 0 280 101 553 642.2 0 553 102 119 642.2 0 119 103 120 642.2 0 120 104 159 642.2 0 159 105 184 642.2 0 184 106 191 642.2 0 191 107 337 642.2 0 337 108 193 642.2 0 193 109 287 642.2 0 287 110 384 642.2 0 384 111 383 642.2 0 383 112 1493 642.2 0 1493 113 7062 642.2 0 7062 114 2903 642.2 0 2903 115 16657 642.2 0 16657 116 156 642.2 0 156 117 134 642.2 0 134 118 88 642.2 0 88 119 164 642.2 0 164 120 134 642.2 0 134 121 128 642.2 0 128 122 113 642.2 0 113 123 143 642.2 0 143 124 280 642.2 0 280 125 181 642.2 0 181 126 138 642.2 0 138 127 138 642.2 0 138 128 268 642.2 0 268 129 226 642.2 0 226 130 547 642.2 0 547 131 469 642.2 0 469 132 126 642.2 0 126 133 131 642.2 0 131 134 584 642.2 0 584 135 117 642.2 0 117 136 134 642.2 0 134 137 108 642.2 0 108 138 201 642.2 0 201 139 134 642.2 0 134 140 158 642.2 0 158 141 154 642.2 0 154 142 272 642.2 0 272 143 203 642.2 0 203 144 450 642.2 0 450 145 144 642.2 0 144 146 131 642.2 0 131 147 153 642.2 0 153 148 96 642.2 0 96 149 135 642.2 0 135 150 141 642.2 0 141 151 108 642.2 0 108 152 121 642.2 0 121 153 146 642.2 0 146 154 433 642.2 0 433 155 872 642.2 0 872 156 1465 642.2 0 1465 157 633 642.2 0 633 158 187 642.2 0 187 159 90 642.2 0 90 160 86 642.2 0 86 161 126 642.2 0 126 162 97 642.2 0 97 163 132 642.2 0 132 164 223 642.2 0 223 165 118 642.2 0 118 166 143 642.2 0 143 167 414 642.2 0 414 168 241 642.2 0 241 169 116 642.2 0 116 170 91 642.2 0 91 171 282 642.2 0 282 172 166 642.2 0 166 173 100 642.2 0 100 174 110 642.2 0 110 175 116 642.2 0 116 176 96 642.2 0 96 177 107 642.2 0 107 178 230 642.2 0 230 179 131 642.2 0 131 180 144 642.2 0 144 181 86 642.2 0 86 182 105 642.2 0 105 183 112 642.2 0 112 184 129 642.2 0 129 185 96 642.2 0 96 186 126 642.2 0 126 187 127 642.2 0 127 188 117 642.2 0 117 189 345 642.2 0 345 190 118 642.2 0 118 191 125 642.2 0 125 192 94 642.2 0 94 193 133 642.2 0 133 194 99 642.2 0 99 195 86 642.2 0 86 196 137 642.2 0 137 197 178 642.2 0 178 198 107 642.2 0 107 199 108 642.2 0 108 200 163 642.2 0 163 201 390 642.2 0 390 202 564 642.2 0 564 203 91 642.2 0 91 204 120 642.2 0 120 205 125 642.2 0 125 206 215 642.2 0 215 207 127 642.2 0 127 208 170 642.2 0 170 209 107 642.2 0 107 210 144 642.2 0 144 211 438 642.2 0 438 212 152 642.2 0 152 213 201 642.2 0 201 214 105 642.2 0 105 215 95 642.2 0 95 216 115 642.2 0 115 217 96 642.2 0 96 218 123 642.2 0 123 219 112 642.2 0 112 220 91 642.2 0 91 221 111 642.2 0 111 222 131 642.2 0 131 223 125 642.2 0 125 224 359 642.2 0 359 225 127 642.2 0 127 226 135 642.2 0 135 227 143 642.2 0 143 228 262 642.2 0 262 229 183 642.2 0 183 230 372 642.2 0 372 231 133 642.2 0 133 232 148 642.2 0 148 233 112 642.2 0 112 234 130 642.2 0 130 235 169 642.2 0 169 236 138 642.2 0 138 237 147 642.2 0 147 238 95 642.2 0 95 239 151 642.2 0 151 240 124 642.2 0 124 241 102 642.2 0 102 242 144 642.2 0 144 243 131 642.2 0 131 244 87 642.2 0 87 245 103 642.2 0 103 246 94 642.2 0 94 247 136 642.2 0 136 248 142 642.2 0 142 249 215 642.2 0 215 250 116 642.2 0 116 251 91 642.2 0 91 252 101 642.2 0 101 253 103 642.2 0 103 254 124 642.2 0 124 255 135 642.2 0 135 256 175 642.2 0 175 257 148 642.2 0 148 258 166 642.2 0 166 259 235 642.2 0 235 260 104 642.2 0 104 261 111 642.2 0 111 262 93 642.2 0 93 263 112 642.2 0 112 264 118 642.2 0 118 265 115 642.2 0 115 266 148 642.2 0 148 267 98 642.2 0 98 268 178 642.2 0 178 269 219 642.2 0 219 270 294 642.2 0 294 271 159 642.2 0 159 272 102 642.2 0 102 273 147 642.2 0 147 274 154 642.2 0 154 275 124 642.2 0 124 276 135 642.2 0 135 277 110 642.2 0 110 278 140 642.2 0 140 279 110 642.2 0 110 280 349 642.2 0 349 281 170 642.2 0 170 282 152 642.2 0 152 283 146 642.2 0 146 284 111 642.2 0 111 285 202 642.2 0 202 286 167 642.2 0 167 287 133 642.2 0 133 288 238 642.2 0 238 289 162 642.2 0 162 290 189 642.2 0 189 291 243 642.2 0 243 292 285 642.2 0 285 293 270 642.2 0 270 294 531 642.2 0 531 295 1004 642.2 0 1004 296 2055 642.2 0 2055 297 1556 642.2 0 1556 298 25441 642.2 0 25441 299 4745 642.2 0 4745 300 10176 642.2 0 10176 301 185 642.2 0 185 Finished in 11.51 s (49 us/read; 1.23 M reads/minute). === Summary === Total reads processed: 235,490 Reads with adapters: 22,921 (9.7%) Reads that were too short: 43 (0.0%) Reads written (passing filters): 22,878 (9.7%) Total basepairs processed: 61,766,190 bp Total written (filtered): 6,195,450 bp (10.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 22921 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 7 0.0 3 0 0 7 19 281 0.0 3 0 277 3 1 20 22219 0.0 4 21841 363 13 2 21 64 0.0 4 13 46 3 1 1 22 7 0.0 4 1 0 6 23 13 0.0 4 0 0 0 12 1 24 2 0.0 4 1 0 0 0 1 25 1 0.0 4 1 29 1 0.0 4 1 30 1 0.0 4 1 40 1 0.0 4 1 50 2 0.0 4 0 0 0 0 2 51 1 0.0 4 0 0 0 0 1 63 1 0.0 4 0 0 1 77 1 0.0 4 0 0 0 1 91 1 0.0 4 0 0 0 0 1 93 1 0.0 4 1 94 1 0.0 4 1 97 1 0.0 4 0 0 1 102 1 0.0 4 1 106 1 0.0 4 1 115 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 1 121 6 0.0 4 0 0 0 0 6 122 1 0.0 4 1 123 1 0.0 4 0 1 125 1 0.0 4 0 0 0 0 1 126 3 0.0 4 0 0 0 0 3 127 259 0.0 4 0 0 0 0 259 128 2 0.0 4 0 0 0 0 2 129 1 0.0 4 0 0 0 0 1 141 1 0.0 4 1 151 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 1 154 1 0.0 4 0 0 0 0 1 156 1 0.0 4 0 0 0 0 1 162 1 0.0 4 0 0 1 169 1 0.0 4 0 0 0 0 1 170 1 0.0 4 0 0 0 0 1 171 4 0.0 4 0 0 0 0 4 174 1 0.0 4 0 0 1 175 3 0.0 4 0 1 0 0 2 176 3 0.0 4 1 1 0 0 1 178 1 0.0 4 0 1 179 1 0.0 4 0 0 0 0 1 186 1 0.0 4 0 0 0 0 1 193 1 0.0 4 0 1 197 1 0.0 4 0 1 202 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 214 2 0.0 4 0 0 0 1 1 215 1 0.0 4 0 0 0 0 1 224 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 245 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 294 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.82 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 22,878 Reads with adapters: 3,197 (14.0%) Reads written (passing filters): 22,878 (100.0%) Total basepairs processed: 6,195,450 bp Total written (filtered): 6,162,810 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3197 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.8% C: 2.9% G: 95.0% T: 1.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 7 357.5 0 7 4 3 89.4 0 3 6 2 5.6 0 2 8 2 0.3 0 2 9 1373 0.1 0 43 1330 10 1589 0.0 1 42 1547 11 204 0.0 1 2 202 12 5 0.0 1 0 5 13 1 0.0 1 0 1 14 1 0.0 1 0 1 33 1 0.0 1 1 152 1 0.0 1 0 1 169 1 0.0 1 0 1 171 1 0.0 1 1 174 1 0.0 1 1 223 1 0.0 1 1 267 1 0.0 1 1 270 2 0.0 1 2 272 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.