This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GAAGCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 70.62 s (27 us/read; 2.23 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 321,273 (12.2%) Reads that were too short: 13,590 (0.5%) Reads written (passing filters): 307,683 (11.7%) Total basepairs processed: 791,719,999 bp Total written (filtered): 81,020,220 bp (10.2%) === Adapter 1 === Sequence: GAAGCT; Type: regular 5'; Length: 6; Trimmed: 321273 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1544 642.2 0 1544 7 5885 642.2 0 5885 8 115223 642.2 0 115223 9 101718 642.2 0 101718 10 169 642.2 0 169 11 56 642.2 0 56 12 57 642.2 0 57 13 49 642.2 0 49 14 46 642.2 0 46 15 37 642.2 0 37 16 57 642.2 0 57 17 41 642.2 0 41 18 48 642.2 0 48 19 62 642.2 0 62 20 58 642.2 0 58 21 63 642.2 0 63 22 62 642.2 0 62 23 90 642.2 0 90 24 106 642.2 0 106 25 129 642.2 0 129 26 102 642.2 0 102 27 68 642.2 0 68 28 60 642.2 0 60 29 50 642.2 0 50 30 82 642.2 0 82 31 86 642.2 0 86 32 68 642.2 0 68 33 67 642.2 0 67 34 137 642.2 0 137 35 124 642.2 0 124 36 210 642.2 0 210 37 228 642.2 0 228 38 57 642.2 0 57 39 95 642.2 0 95 40 47 642.2 0 47 41 57 642.2 0 57 42 78 642.2 0 78 43 49 642.2 0 49 44 50 642.2 0 50 45 72 642.2 0 72 46 89 642.2 0 89 47 379 642.2 0 379 48 94 642.2 0 94 49 124 642.2 0 124 50 57 642.2 0 57 51 80 642.2 0 80 52 68 642.2 0 68 53 138 642.2 0 138 54 215 642.2 0 215 55 236 642.2 0 236 56 104 642.2 0 104 57 73 642.2 0 73 58 51 642.2 0 51 59 30 642.2 0 30 60 74 642.2 0 74 61 112 642.2 0 112 62 92 642.2 0 92 63 106 642.2 0 106 64 213 642.2 0 213 65 79 642.2 0 79 66 83 642.2 0 83 67 91 642.2 0 91 68 79 642.2 0 79 69 101 642.2 0 101 70 80 642.2 0 80 71 78 642.2 0 78 72 96 642.2 0 96 73 82 642.2 0 82 74 160 642.2 0 160 75 150 642.2 0 150 76 254 642.2 0 254 77 760 642.2 0 760 78 723 642.2 0 723 79 902 642.2 0 902 80 526 642.2 0 526 81 5054 642.2 0 5054 82 7009 642.2 0 7009 83 4552 642.2 0 4552 84 140 642.2 0 140 85 301 642.2 0 301 86 270 642.2 0 270 87 594 642.2 0 594 88 87 642.2 0 87 89 164 642.2 0 164 90 35 642.2 0 35 91 39 642.2 0 39 92 57 642.2 0 57 93 87 642.2 0 87 94 97 642.2 0 97 95 81 642.2 0 81 96 62 642.2 0 62 97 57 642.2 0 57 98 76 642.2 0 76 99 53 642.2 0 53 100 64 642.2 0 64 101 83 642.2 0 83 102 187 642.2 0 187 103 83 642.2 0 83 104 174 642.2 0 174 105 51 642.2 0 51 106 77 642.2 0 77 107 62 642.2 0 62 108 112 642.2 0 112 109 116 642.2 0 116 110 74 642.2 0 74 111 122 642.2 0 122 112 130 642.2 0 130 113 78 642.2 0 78 114 86 642.2 0 86 115 102 642.2 0 102 116 94 642.2 0 94 117 159 642.2 0 159 118 245 642.2 0 245 119 568 642.2 0 568 120 372 642.2 0 372 121 942 642.2 0 942 122 12080 642.2 0 12080 123 6104 642.2 0 6104 124 14748 642.2 0 14748 125 190 642.2 0 190 126 68 642.2 0 68 127 76 642.2 0 76 128 225 642.2 0 225 129 70 642.2 0 70 130 87 642.2 0 87 131 50 642.2 0 50 132 64 642.2 0 64 133 120 642.2 0 120 134 78 642.2 0 78 135 101 642.2 0 101 136 67 642.2 0 67 137 57 642.2 0 57 138 64 642.2 0 64 139 76 642.2 0 76 140 68 642.2 0 68 141 78 642.2 0 78 142 141 642.2 0 141 143 170 642.2 0 170 144 598 642.2 0 598 145 200 642.2 0 200 146 356 642.2 0 356 147 135 642.2 0 135 148 391 642.2 0 391 149 357 642.2 0 357 150 120 642.2 0 120 151 428 642.2 0 428 152 85 642.2 0 85 153 77 642.2 0 77 154 97 642.2 0 97 155 80 642.2 0 80 156 61 642.2 0 61 157 47 642.2 0 47 158 67 642.2 0 67 159 60 642.2 0 60 160 80 642.2 0 80 161 61 642.2 0 61 162 64 642.2 0 64 163 49 642.2 0 49 164 48 642.2 0 48 165 61 642.2 0 61 166 62 642.2 0 62 167 158 642.2 0 158 168 93 642.2 0 93 169 64 642.2 0 64 170 83 642.2 0 83 171 52 642.2 0 52 172 63 642.2 0 63 173 62 642.2 0 62 174 57 642.2 0 57 175 84 642.2 0 84 176 53 642.2 0 53 177 75 642.2 0 75 178 124 642.2 0 124 179 85 642.2 0 85 180 118 642.2 0 118 181 563 642.2 0 563 182 4605 642.2 0 4605 183 2851 642.2 0 2851 184 1924 642.2 0 1924 185 105 642.2 0 105 186 71 642.2 0 71 187 74 642.2 0 74 188 74 642.2 0 74 189 55 642.2 0 55 190 73 642.2 0 73 191 41 642.2 0 41 192 61 642.2 0 61 193 90 642.2 0 90 194 166 642.2 0 166 195 147 642.2 0 147 196 78 642.2 0 78 197 54 642.2 0 54 198 141 642.2 0 141 199 152 642.2 0 152 200 90 642.2 0 90 201 69 642.2 0 69 202 57 642.2 0 57 203 45 642.2 0 45 204 52 642.2 0 52 205 101 642.2 0 101 206 353 642.2 0 353 207 63 642.2 0 63 208 45 642.2 0 45 209 86 642.2 0 86 210 87 642.2 0 87 211 86 642.2 0 86 212 71 642.2 0 71 213 54 642.2 0 54 214 56 642.2 0 56 215 41 642.2 0 41 216 35 642.2 0 35 217 55 642.2 0 55 218 55 642.2 0 55 219 77 642.2 0 77 220 60 642.2 0 60 221 83 642.2 0 83 222 48 642.2 0 48 223 56 642.2 0 56 224 57 642.2 0 57 225 50 642.2 0 50 226 113 642.2 0 113 227 72 642.2 0 72 228 114 642.2 0 114 229 78 642.2 0 78 230 89 642.2 0 89 231 66 642.2 0 66 232 46 642.2 0 46 233 61 642.2 0 61 234 58 642.2 0 58 235 65 642.2 0 65 236 68 642.2 0 68 237 55 642.2 0 55 238 84 642.2 0 84 239 81 642.2 0 81 240 235 642.2 0 235 241 365 642.2 0 365 242 158 642.2 0 158 243 88 642.2 0 88 244 56 642.2 0 56 245 63 642.2 0 63 246 42 642.2 0 42 247 80 642.2 0 80 248 50 642.2 0 50 249 55 642.2 0 55 250 58 642.2 0 58 251 63 642.2 0 63 252 75 642.2 0 75 253 116 642.2 0 116 254 109 642.2 0 109 255 104 642.2 0 104 256 100 642.2 0 100 257 88 642.2 0 88 258 96 642.2 0 96 259 92 642.2 0 92 260 101 642.2 0 101 261 142 642.2 0 142 262 87 642.2 0 87 263 88 642.2 0 88 264 78 642.2 0 78 265 79 642.2 0 79 266 1010 642.2 0 1010 267 404 642.2 0 404 268 1215 642.2 0 1215 269 257 642.2 0 257 270 71 642.2 0 71 271 52 642.2 0 52 272 81 642.2 0 81 273 56 642.2 0 56 274 166 642.2 0 166 275 173 642.2 0 173 276 180 642.2 0 180 277 158 642.2 0 158 278 299 642.2 0 299 279 263 642.2 0 263 280 132 642.2 0 132 281 207 642.2 0 207 282 78 642.2 0 78 283 114 642.2 0 114 284 49 642.2 0 49 285 58 642.2 0 58 286 106 642.2 0 106 287 165 642.2 0 165 288 724 642.2 0 724 289 207 642.2 0 207 290 273 642.2 0 273 291 85 642.2 0 85 292 125 642.2 0 125 293 141 642.2 0 141 294 205 642.2 0 205 295 119 642.2 0 119 296 214 642.2 0 214 297 217 642.2 0 217 298 151 642.2 0 151 299 94 642.2 0 94 300 126 642.2 0 126 301 124 642.2 0 124 Finished in 13.23 s (43 us/read; 1.40 M reads/minute). === Summary === Total reads processed: 307,683 Reads with adapters: 117,746 (38.3%) Reads that were too short: 511 (0.2%) Reads written (passing filters): 117,235 (38.1%) Total basepairs processed: 81,020,220 bp Total written (filtered): 32,193,198 bp (39.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 117746 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 27 0.0 2 0 0 0 27 15 103 0.0 3 0 0 71 32 16 1090 0.0 3 0 878 133 79 17 113957 0.0 3 103556 8299 1666 436 18 303 0.0 3 13 238 35 17 19 457 0.0 3 1 1 278 177 20 142 0.0 3 1 0 0 141 21 1 0.0 3 1 22 22 0.0 3 0 0 0 22 25 125 0.0 3 0 0 103 22 27 3 0.0 3 0 0 0 3 28 5 0.0 3 1 0 0 4 29 3 0.0 3 0 0 1 2 30 3 0.0 3 2 0 0 1 34 133 0.0 3 0 0 0 133 35 1 0.0 3 0 0 0 1 37 1 0.0 3 1 38 3 0.0 3 0 0 0 3 39 1 0.0 3 0 0 0 1 41 2 0.0 3 0 0 0 2 42 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 44 3 0.0 3 0 0 0 3 45 3 0.0 3 0 0 1 2 46 4 0.0 3 0 0 2 2 47 9 0.0 3 0 0 0 9 48 6 0.0 3 0 0 0 6 49 170 0.0 3 0 0 1 169 50 346 0.0 3 0 0 2 344 51 106 0.0 3 0 0 3 103 52 27 0.0 3 0 0 0 27 53 49 0.0 3 0 0 1 48 54 13 0.0 3 0 0 0 13 55 4 0.0 3 0 0 0 4 56 1 0.0 3 0 0 0 1 59 2 0.0 3 0 0 1 1 60 3 0.0 3 0 0 0 3 63 3 0.0 3 0 0 1 2 65 1 0.0 3 0 0 0 1 66 2 0.0 3 0 0 0 2 67 5 0.0 3 0 0 1 4 69 2 0.0 3 0 0 0 2 70 10 0.0 3 0 0 0 10 71 5 0.0 3 0 0 1 4 72 2 0.0 3 0 1 0 1 73 7 0.0 3 0 0 1 6 74 1 0.0 3 0 0 0 1 75 2 0.0 3 0 0 0 2 76 17 0.0 3 0 0 1 16 77 4 0.0 3 0 0 0 4 78 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 82 2 0.0 3 0 0 0 2 83 1 0.0 3 0 0 0 1 87 2 0.0 3 0 0 0 2 89 2 0.0 3 0 0 0 2 90 5 0.0 3 0 0 0 5 91 2 0.0 3 0 0 0 2 93 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 98 3 0.0 3 0 0 0 3 100 6 0.0 3 0 0 0 6 101 15 0.0 3 0 0 1 14 102 10 0.0 3 0 0 1 9 103 7 0.0 3 0 0 1 6 105 1 0.0 3 0 0 0 1 106 24 0.0 3 0 0 0 24 107 3 0.0 3 0 0 0 3 109 7 0.0 3 0 0 0 7 110 9 0.0 3 0 0 0 9 111 3 0.0 3 0 0 0 3 112 2 0.0 3 0 0 0 2 117 3 0.0 3 0 0 0 3 118 5 0.0 3 0 0 0 5 120 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 123 3 0.0 3 0 0 0 3 125 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 134 3 0.0 3 0 0 0 3 135 2 0.0 3 0 0 0 2 136 1 0.0 3 0 0 0 1 137 2 0.0 3 0 0 0 2 138 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 142 2 0.0 3 0 0 0 2 143 3 0.0 3 0 0 0 3 144 2 0.0 3 0 0 0 2 146 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 150 3 0.0 3 0 0 0 3 151 3 0.0 3 0 0 0 3 152 2 0.0 3 0 0 0 2 155 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 157 4 0.0 3 0 0 0 4 158 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 160 2 0.0 3 0 0 0 2 161 29 0.0 3 0 0 0 29 162 67 0.0 3 1 0 3 63 163 27 0.0 3 0 0 0 27 164 49 0.0 3 0 0 4 45 165 82 0.0 3 0 0 7 75 166 22 0.0 3 0 0 2 20 167 3 0.0 3 0 0 0 3 168 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 179 3 0.0 3 0 0 0 3 180 1 0.0 3 0 0 0 1 181 2 0.0 3 0 0 0 2 183 4 0.0 3 0 0 0 4 184 4 0.0 3 0 0 0 4 187 2 0.0 3 0 0 0 2 189 2 0.0 3 0 0 0 2 190 2 0.0 3 0 0 0 2 191 4 0.0 3 0 0 0 4 192 7 0.0 3 0 0 0 7 193 1 0.0 3 0 0 0 1 194 5 0.0 3 0 0 0 5 195 1 0.0 3 0 0 0 1 196 2 0.0 3 0 0 0 2 198 1 0.0 3 0 0 0 1 201 1 0.0 3 0 1 203 3 0.0 3 0 0 0 3 204 1 0.0 3 0 0 0 1 207 2 0.0 3 0 0 0 2 208 3 0.0 3 0 0 0 3 209 1 0.0 3 0 0 0 1 212 1 0.0 3 0 0 0 1 214 1 0.0 3 0 1 216 2 0.0 3 0 0 0 2 217 2 0.0 3 0 0 0 2 220 1 0.0 3 0 0 0 1 221 4 0.0 3 0 0 0 4 222 1 0.0 3 0 0 0 1 223 1 0.0 3 0 0 0 1 225 2 0.0 3 0 0 0 2 229 1 0.0 3 0 0 0 1 235 3 0.0 3 0 0 0 3 237 2 0.0 3 0 0 2 243 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 259 2 0.0 3 0 0 1 1 265 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 273 1 0.0 3 0 0 0 1 275 1 0.0 3 0 0 0 1 277 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 280 7 0.0 3 0 0 0 7 281 2 0.0 3 0 0 0 2 282 4 0.0 3 0 0 0 4 283 4 0.0 3 0 0 1 3 289 3 0.0 3 0 0 1 2 292 3 0.0 3 0 0 0 3 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.15 s (35 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 117,235 Reads with adapters: 337 (0.3%) Reads written (passing filters): 117,235 (100.0%) Total basepairs processed: 32,193,198 bp Total written (filtered): 32,192,045 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 337 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 51.6% C: 13.1% G: 30.9% T: 4.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 228 1831.8 0 228 4 95 457.9 0 95 5 7 114.5 0 7 6 4 28.6 0 4 10 3 0.1 1 0 3