This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GAAGCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 69.85 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 342,011 (13.0%) Reads that were too short: 13,840 (0.5%) Reads written (passing filters): 328,171 (12.5%) Total basepairs processed: 791,719,999 bp Total written (filtered): 86,108,546 bp (10.9%) === Adapter 1 === Sequence: GAAGCT; Type: regular 5'; Length: 6; Trimmed: 342011 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1270 642.2 0 1270 7 4371 642.2 0 4371 8 111230 642.2 0 111230 9 120464 642.2 0 120464 10 202 642.2 0 202 11 64 642.2 0 64 12 46 642.2 0 46 13 69 642.2 0 69 14 53 642.2 0 53 15 45 642.2 0 45 16 76 642.2 0 76 17 55 642.2 0 55 18 78 642.2 0 78 19 69 642.2 0 69 20 49 642.2 0 49 21 61 642.2 0 61 22 64 642.2 0 64 23 74 642.2 0 74 24 60 642.2 0 60 25 54 642.2 0 54 26 60 642.2 0 60 27 61 642.2 0 61 28 68 642.2 0 68 29 64 642.2 0 64 30 53 642.2 0 53 31 85 642.2 0 85 32 72 642.2 0 72 33 84 642.2 0 84 34 91 642.2 0 91 35 93 642.2 0 93 36 100 642.2 0 100 37 278 642.2 0 278 38 78 642.2 0 78 39 132 642.2 0 132 40 67 642.2 0 67 41 91 642.2 0 91 42 88 642.2 0 88 43 75 642.2 0 75 44 79 642.2 0 79 45 79 642.2 0 79 46 106 642.2 0 106 47 428 642.2 0 428 48 117 642.2 0 117 49 131 642.2 0 131 50 88 642.2 0 88 51 92 642.2 0 92 52 95 642.2 0 95 53 128 642.2 0 128 54 257 642.2 0 257 55 144 642.2 0 144 56 110 642.2 0 110 57 79 642.2 0 79 58 67 642.2 0 67 59 79 642.2 0 79 60 71 642.2 0 71 61 135 642.2 0 135 62 91 642.2 0 91 63 167 642.2 0 167 64 269 642.2 0 269 65 65 642.2 0 65 66 107 642.2 0 107 67 101 642.2 0 101 68 89 642.2 0 89 69 119 642.2 0 119 70 103 642.2 0 103 71 120 642.2 0 120 72 113 642.2 0 113 73 90 642.2 0 90 74 169 642.2 0 169 75 166 642.2 0 166 76 320 642.2 0 320 77 980 642.2 0 980 78 777 642.2 0 777 79 881 642.2 0 881 80 411 642.2 0 411 81 6018 642.2 0 6018 82 8518 642.2 0 8518 83 3592 642.2 0 3592 84 137 642.2 0 137 85 195 642.2 0 195 86 144 642.2 0 144 87 229 642.2 0 229 88 101 642.2 0 101 89 167 642.2 0 167 90 62 642.2 0 62 91 71 642.2 0 71 92 90 642.2 0 90 93 111 642.2 0 111 94 97 642.2 0 97 95 91 642.2 0 91 96 90 642.2 0 90 97 78 642.2 0 78 98 134 642.2 0 134 99 109 642.2 0 109 100 56 642.2 0 56 101 127 642.2 0 127 102 125 642.2 0 125 103 87 642.2 0 87 104 148 642.2 0 148 105 75 642.2 0 75 106 68 642.2 0 68 107 105 642.2 0 105 108 149 642.2 0 149 109 155 642.2 0 155 110 93 642.2 0 93 111 159 642.2 0 159 112 180 642.2 0 180 113 102 642.2 0 102 114 109 642.2 0 109 115 173 642.2 0 173 116 155 642.2 0 155 117 161 642.2 0 161 118 267 642.2 0 267 119 328 642.2 0 328 120 316 642.2 0 316 121 625 642.2 0 625 122 13172 642.2 0 13172 123 6743 642.2 0 6743 124 16980 642.2 0 16980 125 132 642.2 0 132 126 99 642.2 0 99 127 96 642.2 0 96 128 224 642.2 0 224 129 103 642.2 0 103 130 97 642.2 0 97 131 70 642.2 0 70 132 63 642.2 0 63 133 100 642.2 0 100 134 72 642.2 0 72 135 113 642.2 0 113 136 59 642.2 0 59 137 73 642.2 0 73 138 91 642.2 0 91 139 72 642.2 0 72 140 80 642.2 0 80 141 71 642.2 0 71 142 141 642.2 0 141 143 198 642.2 0 198 144 608 642.2 0 608 145 224 642.2 0 224 146 209 642.2 0 209 147 165 642.2 0 165 148 204 642.2 0 204 149 276 642.2 0 276 150 117 642.2 0 117 151 214 642.2 0 214 152 72 642.2 0 72 153 67 642.2 0 67 154 87 642.2 0 87 155 80 642.2 0 80 156 62 642.2 0 62 157 55 642.2 0 55 158 62 642.2 0 62 159 54 642.2 0 54 160 86 642.2 0 86 161 79 642.2 0 79 162 80 642.2 0 80 163 75 642.2 0 75 164 81 642.2 0 81 165 85 642.2 0 85 166 59 642.2 0 59 167 184 642.2 0 184 168 123 642.2 0 123 169 77 642.2 0 77 170 73 642.2 0 73 171 65 642.2 0 65 172 80 642.2 0 80 173 74 642.2 0 74 174 55 642.2 0 55 175 85 642.2 0 85 176 78 642.2 0 78 177 90 642.2 0 90 178 160 642.2 0 160 179 113 642.2 0 113 180 131 642.2 0 131 181 486 642.2 0 486 182 5723 642.2 0 5723 183 3727 642.2 0 3727 184 2307 642.2 0 2307 185 79 642.2 0 79 186 88 642.2 0 88 187 77 642.2 0 77 188 79 642.2 0 79 189 49 642.2 0 49 190 77 642.2 0 77 191 55 642.2 0 55 192 79 642.2 0 79 193 65 642.2 0 65 194 69 642.2 0 69 195 77 642.2 0 77 196 50 642.2 0 50 197 59 642.2 0 59 198 68 642.2 0 68 199 119 642.2 0 119 200 116 642.2 0 116 201 70 642.2 0 70 202 82 642.2 0 82 203 84 642.2 0 84 204 82 642.2 0 82 205 89 642.2 0 89 206 448 642.2 0 448 207 61 642.2 0 61 208 73 642.2 0 73 209 67 642.2 0 67 210 68 642.2 0 68 211 72 642.2 0 72 212 77 642.2 0 77 213 79 642.2 0 79 214 89 642.2 0 89 215 81 642.2 0 81 216 55 642.2 0 55 217 63 642.2 0 63 218 76 642.2 0 76 219 98 642.2 0 98 220 93 642.2 0 93 221 87 642.2 0 87 222 63 642.2 0 63 223 70 642.2 0 70 224 82 642.2 0 82 225 94 642.2 0 94 226 82 642.2 0 82 227 74 642.2 0 74 228 86 642.2 0 86 229 66 642.2 0 66 230 65 642.2 0 65 231 54 642.2 0 54 232 54 642.2 0 54 233 63 642.2 0 63 234 73 642.2 0 73 235 78 642.2 0 78 236 66 642.2 0 66 237 99 642.2 0 99 238 103 642.2 0 103 239 67 642.2 0 67 240 229 642.2 0 229 241 349 642.2 0 349 242 179 642.2 0 179 243 77 642.2 0 77 244 72 642.2 0 72 245 62 642.2 0 62 246 57 642.2 0 57 247 61 642.2 0 61 248 77 642.2 0 77 249 68 642.2 0 68 250 71 642.2 0 71 251 96 642.2 0 96 252 109 642.2 0 109 253 212 642.2 0 212 254 94 642.2 0 94 255 107 642.2 0 107 256 129 642.2 0 129 257 84 642.2 0 84 258 104 642.2 0 104 259 74 642.2 0 74 260 87 642.2 0 87 261 108 642.2 0 108 262 92 642.2 0 92 263 84 642.2 0 84 264 85 642.2 0 85 265 109 642.2 0 109 266 1397 642.2 0 1397 267 420 642.2 0 420 268 1536 642.2 0 1536 269 198 642.2 0 198 270 66 642.2 0 66 271 76 642.2 0 76 272 80 642.2 0 80 273 75 642.2 0 75 274 147 642.2 0 147 275 176 642.2 0 176 276 214 642.2 0 214 277 85 642.2 0 85 278 298 642.2 0 298 279 108 642.2 0 108 280 73 642.2 0 73 281 154 642.2 0 154 282 103 642.2 0 103 283 127 642.2 0 127 284 59 642.2 0 59 285 71 642.2 0 71 286 97 642.2 0 97 287 117 642.2 0 117 288 315 642.2 0 315 289 177 642.2 0 177 290 125 642.2 0 125 291 73 642.2 0 73 292 100 642.2 0 100 293 115 642.2 0 115 294 147 642.2 0 147 295 108 642.2 0 108 296 152 642.2 0 152 297 245 642.2 0 245 298 159 642.2 0 159 299 96 642.2 0 96 300 101 642.2 0 101 301 111 642.2 0 111 Finished in 15.45 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 328,171 Reads with adapters: 126,730 (38.6%) Reads that were too short: 48 (0.0%) Reads written (passing filters): 126,682 (38.6%) Total basepairs processed: 86,108,546 bp Total written (filtered): 34,335,350 bp (39.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 126730 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 2 1 18 56 0.0 3 0 0 55 1 19 1651 0.0 3 0 1626 23 2 20 121938 0.0 4 119927 1930 74 6 1 21 222 0.0 4 30 190 2 22 1500 0.0 4 1466 18 15 1 23 98 0.0 4 4 5 0 74 15 24 4 0.0 4 1 0 0 0 3 25 1 0.0 4 0 0 0 1 27 1 0.0 4 1 31 1 0.0 4 1 32 1 0.0 4 1 40 1 0.0 4 1 42 1 0.0 4 1 47 1 0.0 4 1 52 1 0.0 4 0 0 0 0 1 60 1 0.0 4 0 0 0 0 1 75 1 0.0 4 0 0 0 0 1 94 2 0.0 4 0 0 0 0 2 99 2 0.0 4 0 0 0 0 2 124 1 0.0 4 0 0 0 0 1 126 16 0.0 4 0 0 0 0 16 127 1166 0.0 4 0 0 0 0 1166 128 5 0.0 4 0 0 0 0 5 129 5 0.0 4 0 0 0 0 5 130 1 0.0 4 0 0 0 0 1 135 2 0.0 4 0 0 0 0 2 142 1 0.0 4 0 0 0 0 1 143 1 0.0 4 0 0 0 0 1 146 1 0.0 4 0 0 0 0 1 148 1 0.0 4 0 0 0 0 1 149 1 0.0 4 0 0 0 0 1 150 1 0.0 4 0 0 0 0 1 151 1 0.0 4 0 0 0 0 1 156 1 0.0 4 0 0 0 0 1 158 1 0.0 4 0 0 0 0 1 177 1 0.0 4 1 183 1 0.0 4 0 0 1 203 2 0.0 4 0 0 0 0 2 213 1 0.0 4 0 0 0 0 1 226 1 0.0 4 0 0 0 1 228 2 0.0 4 0 0 0 0 2 233 1 0.0 4 0 0 0 0 1 234 2 0.0 4 0 0 0 0 2 235 1 0.0 4 0 0 0 0 1 236 1 0.0 4 0 0 0 0 1 237 2 0.0 4 0 0 0 1 1 239 2 0.0 4 0 0 0 1 1 242 5 0.0 4 0 0 0 1 4 263 1 0.0 4 0 0 0 0 1 282 11 0.0 4 0 0 0 0 11 283 1 0.0 4 0 0 0 0 1 284 1 0.0 4 0 0 0 0 1 285 2 0.0 4 0 0 0 0 2 293 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.89 s (39 us/read; 1.55 M reads/minute). === Summary === Total reads processed: 126,682 Reads with adapters: 52,881 (41.7%) Reads written (passing filters): 126,682 (100.0%) Total basepairs processed: 34,335,350 bp Total written (filtered): 33,852,192 bp (98.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 52881 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.4% C: 1.2% G: 97.7% T: 0.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 28 1979.4 0 28 4 32 494.9 0 32 5 9 123.7 0 9 6 13 30.9 0 13 7 1 7.7 0 1 8 14 1.9 0 14 9 48982 0.5 0 1242 47740 10 3003 0.1 1 113 2890 11 700 0.0 1 4 696 12 76 0.0 1 0 76 13 2 0.0 1 0 2 14 1 0.0 1 0 1 19 1 0.0 1 1 22 1 0.0 1 1 39 1 0.0 1 1 100 1 0.0 1 1 169 11 0.0 1 11 174 1 0.0 1 1 208 1 0.0 1 1 226 1 0.0 1 1 267 1 0.0 1 1 270 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.