This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCCGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 62.47 s (26 us/read; 2.35 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 479,659 (19.6%) Reads that were too short: 387,359 (15.9%) Reads written (passing filters): 92,300 (3.8%) Total basepairs processed: 735,190,694 bp Total written (filtered): 15,640,590 bp (2.1%) === Adapter 1 === Sequence: GTCCGG; Type: regular 5'; Length: 6; Trimmed: 479659 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1072 596.3 0 1072 7 8250 596.3 0 8250 8 349 596.3 0 349 9 10430 596.3 0 10430 10 210 596.3 0 210 11 2388 596.3 0 2388 12 29 596.3 0 29 13 25 596.3 0 25 14 35 596.3 0 35 15 20 596.3 0 20 16 28 596.3 0 28 17 26 596.3 0 26 18 37 596.3 0 37 19 30 596.3 0 30 20 18 596.3 0 18 21 27 596.3 0 27 22 22 596.3 0 22 23 23 596.3 0 23 24 24 596.3 0 24 25 21 596.3 0 21 26 12 596.3 0 12 27 15 596.3 0 15 28 26 596.3 0 26 29 31 596.3 0 31 30 15 596.3 0 15 31 158 596.3 0 158 32 57 596.3 0 57 33 29 596.3 0 29 34 56 596.3 0 56 35 70 596.3 0 70 36 34 596.3 0 34 37 21 596.3 0 21 38 36 596.3 0 36 39 34 596.3 0 34 40 23 596.3 0 23 41 43 596.3 0 43 42 38 596.3 0 38 43 29 596.3 0 29 44 21 596.3 0 21 45 27 596.3 0 27 46 15 596.3 0 15 47 28 596.3 0 28 48 33 596.3 0 33 49 25 596.3 0 25 50 27 596.3 0 27 51 21 596.3 0 21 52 25 596.3 0 25 53 20 596.3 0 20 54 29 596.3 0 29 55 19 596.3 0 19 56 34 596.3 0 34 57 52 596.3 0 52 58 41 596.3 0 41 59 55 596.3 0 55 60 41 596.3 0 41 61 38 596.3 0 38 62 18 596.3 0 18 63 32 596.3 0 32 64 27 596.3 0 27 65 126 596.3 0 126 66 55 596.3 0 55 67 109 596.3 0 109 68 20 596.3 0 20 69 36 596.3 0 36 70 35 596.3 0 35 71 93 596.3 0 93 72 42 596.3 0 42 73 27 596.3 0 27 74 40 596.3 0 40 75 134 596.3 0 134 76 174 596.3 0 174 77 233 596.3 0 233 78 43 596.3 0 43 79 34 596.3 0 34 80 15 596.3 0 15 81 29 596.3 0 29 82 27 596.3 0 27 83 33 596.3 0 33 84 17 596.3 0 17 85 28 596.3 0 28 86 23 596.3 0 23 87 30 596.3 0 30 88 38 596.3 0 38 89 59 596.3 0 59 90 44 596.3 0 44 91 67 596.3 0 67 92 63 596.3 0 63 93 87 596.3 0 87 94 31 596.3 0 31 95 46 596.3 0 46 96 40 596.3 0 40 97 31 596.3 0 31 98 40 596.3 0 40 99 40 596.3 0 40 100 96 596.3 0 96 101 31 596.3 0 31 102 34 596.3 0 34 103 54 596.3 0 54 104 36 596.3 0 36 105 41 596.3 0 41 106 56 596.3 0 56 107 58 596.3 0 58 108 53 596.3 0 53 109 49 596.3 0 49 110 59 596.3 0 59 111 96 596.3 0 96 112 183 596.3 0 183 113 1686 596.3 0 1686 114 2858 596.3 0 2858 115 1883 596.3 0 1883 116 103 596.3 0 103 117 40 596.3 0 40 118 46 596.3 0 46 119 29 596.3 0 29 120 32 596.3 0 32 121 60 596.3 0 60 122 75 596.3 0 75 123 90 596.3 0 90 124 73 596.3 0 73 125 58 596.3 0 58 126 44 596.3 0 44 127 72 596.3 0 72 128 561 596.3 0 561 129 126 596.3 0 126 130 385 596.3 0 385 131 49 596.3 0 49 132 48 596.3 0 48 133 57 596.3 0 57 134 101 596.3 0 101 135 59 596.3 0 59 136 59 596.3 0 59 137 95 596.3 0 95 138 65 596.3 0 65 139 66 596.3 0 66 140 82 596.3 0 82 141 74 596.3 0 74 142 102 596.3 0 102 143 317 596.3 0 317 144 425 596.3 0 425 145 175 596.3 0 175 146 170 596.3 0 170 147 2183 596.3 0 2183 148 1988 596.3 0 1988 149 3091 596.3 0 3091 150 190 596.3 0 190 151 63 596.3 0 63 152 47 596.3 0 47 153 56 596.3 0 56 154 61 596.3 0 61 155 85 596.3 0 85 156 54 596.3 0 54 157 53 596.3 0 53 158 87 596.3 0 87 159 52 596.3 0 52 160 59 596.3 0 59 161 52 596.3 0 52 162 76 596.3 0 76 163 54 596.3 0 54 164 65 596.3 0 65 165 58 596.3 0 58 166 46 596.3 0 46 167 54 596.3 0 54 168 110 596.3 0 110 169 155 596.3 0 155 170 433 596.3 0 433 171 59 596.3 0 59 172 66 596.3 0 66 173 85 596.3 0 85 174 295 596.3 0 295 175 67 596.3 0 67 176 103 596.3 0 103 177 85 596.3 0 85 178 132 596.3 0 132 179 150 596.3 0 150 180 154 596.3 0 154 181 254 596.3 0 254 182 191 596.3 0 191 183 151 596.3 0 151 184 122 596.3 0 122 185 197 596.3 0 197 186 236 596.3 0 236 187 387 596.3 0 387 188 316 596.3 0 316 189 1191 596.3 0 1191 190 1661 596.3 0 1661 191 2296 596.3 0 2296 192 1159 596.3 0 1159 193 813 596.3 0 813 194 623 596.3 0 623 195 1022 596.3 0 1022 196 813 596.3 0 813 197 742 596.3 0 742 198 1008 596.3 0 1008 199 2488 596.3 0 2488 200 10620 596.3 0 10620 201 18223 596.3 0 18223 202 16510 596.3 0 16510 203 2142 596.3 0 2142 204 2126 596.3 0 2126 205 18112 596.3 0 18112 206 39119 596.3 0 39119 207 51113 596.3 0 51113 208 26505 596.3 0 26505 209 8539 596.3 0 8539 210 3151 596.3 0 3151 211 2589 596.3 0 2589 212 1848 596.3 0 1848 213 1398 596.3 0 1398 214 654 596.3 0 654 215 1329 596.3 0 1329 216 1890 596.3 0 1890 217 932 596.3 0 932 218 1638 596.3 0 1638 219 3127 596.3 0 3127 220 2575 596.3 0 2575 221 3473 596.3 0 3473 222 3303 596.3 0 3303 223 1682 596.3 0 1682 224 2282 596.3 0 2282 225 12886 596.3 0 12886 226 39612 596.3 0 39612 227 30667 596.3 0 30667 228 14232 596.3 0 14232 229 126 596.3 0 126 230 50 596.3 0 50 231 58 596.3 0 58 232 44 596.3 0 44 233 28 596.3 0 28 234 67 596.3 0 67 235 81 596.3 0 81 236 135 596.3 0 135 237 103 596.3 0 103 238 156 596.3 0 156 239 79 596.3 0 79 240 83 596.3 0 83 241 98 596.3 0 98 242 81 596.3 0 81 243 232 596.3 0 232 244 1030 596.3 0 1030 245 1355 596.3 0 1355 246 1327 596.3 0 1327 247 312 596.3 0 312 248 395 596.3 0 395 249 896 596.3 0 896 250 592 596.3 0 592 251 705 596.3 0 705 252 1580 596.3 0 1580 253 419 596.3 0 419 254 632 596.3 0 632 255 1599 596.3 0 1599 256 630 596.3 0 630 257 556 596.3 0 556 258 575 596.3 0 575 259 1205 596.3 0 1205 260 1328 596.3 0 1328 261 1640 596.3 0 1640 262 15679 596.3 0 15679 263 28042 596.3 0 28042 264 22290 596.3 0 22290 265 630 596.3 0 630 266 239 596.3 0 239 267 58 596.3 0 58 268 49 596.3 0 49 269 151 596.3 0 151 270 321 596.3 0 321 271 501 596.3 0 501 272 159 596.3 0 159 273 30 596.3 0 30 274 73 596.3 0 73 275 132 596.3 0 132 276 122 596.3 0 122 277 102 596.3 0 102 278 100 596.3 0 100 279 73 596.3 0 73 280 62 596.3 0 62 281 55 596.3 0 55 282 132 596.3 0 132 283 282 596.3 0 282 284 284 596.3 0 284 285 133 596.3 0 133 286 106 596.3 0 106 287 113 596.3 0 113 288 82 596.3 0 82 289 97 596.3 0 97 290 66 596.3 0 66 291 83 596.3 0 83 292 675 596.3 0 675 293 1319 596.3 0 1319 294 1340 596.3 0 1340 295 833 596.3 0 833 296 291 596.3 0 291 297 324 596.3 0 324 298 283 596.3 0 283 299 152 596.3 0 152 300 104 596.3 0 104 301 161 596.3 0 161 Finished in 3.25 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 92,300 Reads with adapters: 11,627 (12.6%) Reads that were too short: 610 (0.7%) Reads written (passing filters): 11,017 (11.9%) Total basepairs processed: 15,640,590 bp Total written (filtered): 2,995,385 bp (19.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 11627 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 5 0.0 2 0 0 0 5 15 9 0.0 3 0 0 7 2 16 158 0.0 3 0 144 12 2 17 10583 0.0 3 9847 595 105 36 18 43 0.0 3 5 21 3 14 19 15 0.0 3 0 1 7 7 20 13 0.0 3 0 0 1 12 21 2 0.0 3 0 0 0 2 22 1 0.0 3 0 1 25 2 0.0 3 0 1 0 1 26 220 0.0 3 0 0 3 217 27 4 0.0 3 0 0 0 4 29 3 0.0 3 0 0 0 3 33 3 0.0 3 0 0 0 3 38 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 44 3 0.0 3 0 0 0 3 45 1 0.0 3 0 0 0 1 46 2 0.0 3 0 0 0 2 47 6 0.0 3 0 0 0 6 48 14 0.0 3 0 0 0 14 49 3 0.0 3 0 0 0 3 51 2 0.0 3 0 0 0 2 53 6 0.0 3 0 0 0 6 56 4 0.0 3 0 0 0 4 57 2 0.0 3 0 0 0 2 58 9 0.0 3 0 0 1 8 59 1 0.0 3 0 0 0 1 61 2 0.0 3 0 0 0 2 62 2 0.0 3 0 0 0 2 63 3 0.0 3 0 0 0 3 65 5 0.0 3 0 0 1 4 66 2 0.0 3 0 0 0 2 67 3 0.0 3 0 0 0 3 68 3 0.0 3 0 1 0 2 69 8 0.0 3 0 0 0 8 70 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 75 39 0.0 3 0 0 0 39 76 77 0.0 3 0 0 3 74 77 8 0.0 3 0 0 0 8 78 112 0.0 3 0 0 1 111 79 7 0.0 3 0 0 1 6 80 4 0.0 3 0 0 0 4 81 30 0.0 3 0 0 1 29 82 4 0.0 3 0 0 1 3 83 2 0.0 3 0 0 0 2 85 2 0.0 3 0 0 0 2 87 2 0.0 3 0 0 0 2 90 1 0.0 3 0 0 0 1 91 3 0.0 3 0 0 1 2 92 1 0.0 3 0 0 0 1 94 4 0.0 3 0 0 0 4 95 2 0.0 3 0 0 0 2 96 7 0.0 3 0 0 0 7 97 6 0.0 3 0 0 1 5 98 9 0.0 3 0 0 1 8 99 2 0.0 3 0 0 0 2 100 3 0.0 3 0 0 0 3 104 5 0.0 3 0 0 0 5 106 28 0.0 3 0 0 2 26 107 6 0.0 3 0 0 0 6 108 2 0.0 3 0 0 0 2 109 1 0.0 3 0 0 0 1 110 2 0.0 3 0 0 0 2 113 9 0.0 3 0 0 0 9 114 3 0.0 3 0 0 0 3 115 2 0.0 3 0 0 0 2 116 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 123 7 0.0 3 0 0 0 7 126 1 0.0 3 0 0 0 1 129 3 0.0 3 0 0 0 3 133 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 138 2 0.0 3 0 0 0 2 140 1 0.0 3 0 0 0 1 141 2 0.0 3 0 0 0 2 142 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 148 2 0.0 3 0 0 0 2 149 1 0.0 3 0 0 0 1 150 3 0.0 3 0 0 0 3 151 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 154 4 0.0 3 0 0 0 4 155 2 0.0 3 0 0 0 2 156 1 0.0 3 0 0 0 1 157 4 0.0 3 0 0 0 4 160 1 0.0 3 0 0 0 1 162 4 0.0 3 0 0 0 4 163 1 0.0 3 0 0 0 1 170 2 0.0 3 0 0 0 2 172 2 0.0 3 0 0 0 2 175 1 0.0 3 0 0 1 178 1 0.0 3 0 0 0 1 179 3 0.0 3 0 0 0 3 180 6 0.0 3 0 0 0 6 181 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 1 185 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 0 1 199 1 0.0 3 0 0 1 200 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 0 1 212 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 223 1 0.0 3 0 0 1 224 1 0.0 3 0 0 0 1 226 2 0.0 3 0 0 0 2 227 2 0.0 3 0 0 0 2 228 2 0.0 3 0 0 0 2 263 1 0.0 3 0 0 0 1 267 1 0.0 3 0 0 0 1 268 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 277 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.40 s (36 us/read; 1.65 M reads/minute). === Summary === Total reads processed: 11,017 Reads with adapters: 272 (2.5%) Reads written (passing filters): 11,017 (100.0%) Total basepairs processed: 2,995,385 bp Total written (filtered): 2,994,565 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 272 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 27.2% C: 48.2% G: 22.8% T: 1.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 268 172.1 0 268 4 4 43.0 0 4