This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCCGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 62.23 s (25 us/read; 2.35 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 466,194 (19.1%) Reads that were too short: 374,090 (15.3%) Reads written (passing filters): 92,104 (3.8%) Total basepairs processed: 735,190,694 bp Total written (filtered): 15,653,518 bp (2.1%) === Adapter 1 === Sequence: GTCCGG; Type: regular 5'; Length: 6; Trimmed: 466194 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1122 596.3 0 1122 7 10044 596.3 0 10044 8 248 596.3 0 248 9 9281 596.3 0 9281 10 146 596.3 0 146 11 2916 596.3 0 2916 12 34 596.3 0 34 13 34 596.3 0 34 14 22 596.3 0 22 15 15 596.3 0 15 16 19 596.3 0 19 17 33 596.3 0 33 18 29 596.3 0 29 19 33 596.3 0 33 20 26 596.3 0 26 21 36 596.3 0 36 22 28 596.3 0 28 23 17 596.3 0 17 24 20 596.3 0 20 25 25 596.3 0 25 26 19 596.3 0 19 27 18 596.3 0 18 28 21 596.3 0 21 29 29 596.3 0 29 30 19 596.3 0 19 31 77 596.3 0 77 32 79 596.3 0 79 33 33 596.3 0 33 34 57 596.3 0 57 35 73 596.3 0 73 36 30 596.3 0 30 37 34 596.3 0 34 38 86 596.3 0 86 39 30 596.3 0 30 40 26 596.3 0 26 41 36 596.3 0 36 42 25 596.3 0 25 43 29 596.3 0 29 44 33 596.3 0 33 45 31 596.3 0 31 46 20 596.3 0 20 47 24 596.3 0 24 48 20 596.3 0 20 49 23 596.3 0 23 50 25 596.3 0 25 51 16 596.3 0 16 52 27 596.3 0 27 53 29 596.3 0 29 54 37 596.3 0 37 55 21 596.3 0 21 56 23 596.3 0 23 57 45 596.3 0 45 58 34 596.3 0 34 59 62 596.3 0 62 60 51 596.3 0 51 61 35 596.3 0 35 62 16 596.3 0 16 63 20 596.3 0 20 64 22 596.3 0 22 65 72 596.3 0 72 66 28 596.3 0 28 67 55 596.3 0 55 68 18 596.3 0 18 69 42 596.3 0 42 70 36 596.3 0 36 71 101 596.3 0 101 72 33 596.3 0 33 73 19 596.3 0 19 74 34 596.3 0 34 75 113 596.3 0 113 76 85 596.3 0 85 77 142 596.3 0 142 78 29 596.3 0 29 79 30 596.3 0 30 80 23 596.3 0 23 81 30 596.3 0 30 82 31 596.3 0 31 83 15 596.3 0 15 84 35 596.3 0 35 85 26 596.3 0 26 86 31 596.3 0 31 87 25 596.3 0 25 88 32 596.3 0 32 89 38 596.3 0 38 90 31 596.3 0 31 91 45 596.3 0 45 92 42 596.3 0 42 93 26 596.3 0 26 94 33 596.3 0 33 95 47 596.3 0 47 96 31 596.3 0 31 97 25 596.3 0 25 98 32 596.3 0 32 99 45 596.3 0 45 100 69 596.3 0 69 101 36 596.3 0 36 102 41 596.3 0 41 103 58 596.3 0 58 104 32 596.3 0 32 105 30 596.3 0 30 106 60 596.3 0 60 107 51 596.3 0 51 108 47 596.3 0 47 109 41 596.3 0 41 110 54 596.3 0 54 111 74 596.3 0 74 112 74 596.3 0 74 113 1334 596.3 0 1334 114 2426 596.3 0 2426 115 1584 596.3 0 1584 116 64 596.3 0 64 117 32 596.3 0 32 118 45 596.3 0 45 119 32 596.3 0 32 120 41 596.3 0 41 121 87 596.3 0 87 122 78 596.3 0 78 123 66 596.3 0 66 124 59 596.3 0 59 125 49 596.3 0 49 126 45 596.3 0 45 127 85 596.3 0 85 128 615 596.3 0 615 129 137 596.3 0 137 130 509 596.3 0 509 131 33 596.3 0 33 132 44 596.3 0 44 133 43 596.3 0 43 134 84 596.3 0 84 135 67 596.3 0 67 136 54 596.3 0 54 137 83 596.3 0 83 138 49 596.3 0 49 139 66 596.3 0 66 140 55 596.3 0 55 141 77 596.3 0 77 142 103 596.3 0 103 143 293 596.3 0 293 144 418 596.3 0 418 145 133 596.3 0 133 146 102 596.3 0 102 147 2100 596.3 0 2100 148 2089 596.3 0 2089 149 3123 596.3 0 3123 150 152 596.3 0 152 151 59 596.3 0 59 152 40 596.3 0 40 153 28 596.3 0 28 154 53 596.3 0 53 155 63 596.3 0 63 156 63 596.3 0 63 157 66 596.3 0 66 158 62 596.3 0 62 159 42 596.3 0 42 160 51 596.3 0 51 161 80 596.3 0 80 162 88 596.3 0 88 163 70 596.3 0 70 164 55 596.3 0 55 165 63 596.3 0 63 166 44 596.3 0 44 167 48 596.3 0 48 168 109 596.3 0 109 169 121 596.3 0 121 170 597 596.3 0 597 171 40 596.3 0 40 172 59 596.3 0 59 173 74 596.3 0 74 174 198 596.3 0 198 175 58 596.3 0 58 176 94 596.3 0 94 177 77 596.3 0 77 178 82 596.3 0 82 179 159 596.3 0 159 180 125 596.3 0 125 181 215 596.3 0 215 182 185 596.3 0 185 183 141 596.3 0 141 184 141 596.3 0 141 185 186 596.3 0 186 186 255 596.3 0 255 187 414 596.3 0 414 188 314 596.3 0 314 189 1543 596.3 0 1543 190 1738 596.3 0 1738 191 2980 596.3 0 2980 192 1154 596.3 0 1154 193 757 596.3 0 757 194 561 596.3 0 561 195 981 596.3 0 981 196 753 596.3 0 753 197 704 596.3 0 704 198 858 596.3 0 858 199 2350 596.3 0 2350 200 10914 596.3 0 10914 201 18126 596.3 0 18126 202 15932 596.3 0 15932 203 1885 596.3 0 1885 204 1738 596.3 0 1738 205 18574 596.3 0 18574 206 42872 596.3 0 42872 207 55223 596.3 0 55223 208 24768 596.3 0 24768 209 7009 596.3 0 7009 210 3581 596.3 0 3581 211 2894 596.3 0 2894 212 2010 596.3 0 2010 213 1628 596.3 0 1628 214 598 596.3 0 598 215 1315 596.3 0 1315 216 2125 596.3 0 2125 217 1061 596.3 0 1061 218 1790 596.3 0 1790 219 4051 596.3 0 4051 220 2731 596.3 0 2731 221 3561 596.3 0 3561 222 3584 596.3 0 3584 223 1817 596.3 0 1817 224 2073 596.3 0 2073 225 10540 596.3 0 10540 226 41003 596.3 0 41003 227 31887 596.3 0 31887 228 16828 596.3 0 16828 229 85 596.3 0 85 230 22 596.3 0 22 231 37 596.3 0 37 232 40 596.3 0 40 233 23 596.3 0 23 234 28 596.3 0 28 235 45 596.3 0 45 236 80 596.3 0 80 237 41 596.3 0 41 238 144 596.3 0 144 239 54 596.3 0 54 240 64 596.3 0 64 241 59 596.3 0 59 242 55 596.3 0 55 243 143 596.3 0 143 244 596 596.3 0 596 245 468 596.3 0 468 246 503 596.3 0 503 247 188 596.3 0 188 248 339 596.3 0 339 249 636 596.3 0 636 250 352 596.3 0 352 251 332 596.3 0 332 252 872 596.3 0 872 253 243 596.3 0 243 254 644 596.3 0 644 255 2028 596.3 0 2028 256 662 596.3 0 662 257 386 596.3 0 386 258 340 596.3 0 340 259 578 596.3 0 578 260 785 596.3 0 785 261 895 596.3 0 895 262 12503 596.3 0 12503 263 24211 596.3 0 24211 264 19153 596.3 0 19153 265 292 596.3 0 292 266 105 596.3 0 105 267 20 596.3 0 20 268 36 596.3 0 36 269 55 596.3 0 55 270 100 596.3 0 100 271 275 596.3 0 275 272 121 596.3 0 121 273 21 596.3 0 21 274 63 596.3 0 63 275 126 596.3 0 126 276 41 596.3 0 41 277 28 596.3 0 28 278 40 596.3 0 40 279 27 596.3 0 27 280 20 596.3 0 20 281 28 596.3 0 28 282 56 596.3 0 56 283 108 596.3 0 108 284 79 596.3 0 79 285 49 596.3 0 49 286 24 596.3 0 24 287 26 596.3 0 26 288 29 596.3 0 29 289 27 596.3 0 27 290 36 596.3 0 36 291 32 596.3 0 32 292 163 596.3 0 163 293 273 596.3 0 273 294 377 596.3 0 377 295 324 596.3 0 324 296 97 596.3 0 97 297 90 596.3 0 90 298 70 596.3 0 70 299 46 596.3 0 46 300 29 596.3 0 29 301 45 596.3 0 45 Finished in 3.61 s (39 us/read; 1.53 M reads/minute). === Summary === Total reads processed: 92,104 Reads with adapters: 10,892 (11.8%) Reads that were too short: 26 (0.0%) Reads written (passing filters): 10,866 (11.8%) Total basepairs processed: 15,653,518 bp Total written (filtered): 2,977,456 bp (19.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 10892 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 4 0.0 3 0 0 4 19 149 0.0 3 0 145 3 1 20 10668 0.0 4 10482 179 7 21 29 0.0 4 3 24 1 0 1 22 3 0.0 4 0 0 3 23 8 0.0 4 1 0 0 6 1 26 1 0.0 4 0 0 0 0 1 33 1 0.0 4 0 0 0 0 1 34 1 0.0 4 0 0 0 0 1 36 1 0.0 4 0 0 0 0 1 40 1 0.0 4 0 0 0 0 1 64 1 0.0 4 0 0 0 0 1 65 1 0.0 4 0 0 0 0 1 66 2 0.0 4 0 0 0 0 2 67 2 0.0 4 1 0 0 0 1 76 1 0.0 4 0 1 89 1 0.0 4 0 0 0 0 1 91 2 0.0 4 0 0 0 0 2 92 3 0.0 4 0 0 0 0 3 96 1 0.0 4 0 0 0 0 1 98 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 105 2 0.0 4 0 0 0 0 2 114 1 0.0 4 0 0 0 0 1 119 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 0 1 133 1 0.0 4 0 0 0 0 1 134 1 0.0 4 0 0 0 0 1 169 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 255 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.38 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 10,866 Reads with adapters: 663 (6.1%) Reads written (passing filters): 10,866 (100.0%) Total basepairs processed: 2,977,456 bp Total written (filtered): 2,969,628 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 663 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.3% C: 3.2% G: 91.4% T: 2.1% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 23 169.8 0 23 4 6 42.4 0 6 5 3 10.6 0 3 8 2 0.2 0 2 9 5 0.0 0 0 5 10 2 0.0 1 0 2 11 581 0.0 1 5 576 12 31 0.0 1 11 20 13 5 0.0 1 1 4 14 1 0.0 1 0 1 152 1 0.0 1 0 1 195 1 0.0 1 1 225 2 0.0 1 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.