This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCCGCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 64.19 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 109,607 (4.5%) Reads that were too short: 47,961 (2.0%) Reads written (passing filters): 61,646 (2.5%) Total basepairs processed: 735,190,694 bp Total written (filtered): 12,483,161 bp (1.7%) === Adapter 1 === Sequence: TCCGCT; Type: regular 5'; Length: 6; Trimmed: 109607 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 303 596.3 0 303 7 846 596.3 0 846 8 9194 596.3 0 9194 9 7836 596.3 0 7836 10 23 596.3 0 23 11 15 596.3 0 15 12 26 596.3 0 26 13 22 596.3 0 22 14 18 596.3 0 18 15 17 596.3 0 17 16 6 596.3 0 6 17 31 596.3 0 31 18 12 596.3 0 12 19 17 596.3 0 17 20 13 596.3 0 13 21 15 596.3 0 15 22 19 596.3 0 19 23 19 596.3 0 19 24 18 596.3 0 18 25 31 596.3 0 31 26 18 596.3 0 18 27 25 596.3 0 25 28 15 596.3 0 15 29 20 596.3 0 20 30 12 596.3 0 12 31 18 596.3 0 18 32 178 596.3 0 178 33 31 596.3 0 31 34 53 596.3 0 53 35 38 596.3 0 38 36 36 596.3 0 36 37 238 596.3 0 238 38 66 596.3 0 66 39 191 596.3 0 191 40 36 596.3 0 36 41 35 596.3 0 35 42 124 596.3 0 124 43 18 596.3 0 18 44 27 596.3 0 27 45 15 596.3 0 15 46 24 596.3 0 24 47 28 596.3 0 28 48 37 596.3 0 37 49 34 596.3 0 34 50 15 596.3 0 15 51 21 596.3 0 21 52 425 596.3 0 425 53 155 596.3 0 155 54 425 596.3 0 425 55 22 596.3 0 22 56 18 596.3 0 18 57 31 596.3 0 31 58 30 596.3 0 30 59 24 596.3 0 24 60 28 596.3 0 28 61 45 596.3 0 45 62 37 596.3 0 37 63 22 596.3 0 22 64 15 596.3 0 15 65 23 596.3 0 23 66 69 596.3 0 69 67 29 596.3 0 29 68 111 596.3 0 111 69 16 596.3 0 16 70 19 596.3 0 19 71 51 596.3 0 51 72 75 596.3 0 75 73 106 596.3 0 106 74 16 596.3 0 16 75 10 596.3 0 10 76 8 596.3 0 8 77 15 596.3 0 15 78 8 596.3 0 8 79 12 596.3 0 12 80 16 596.3 0 16 81 25 596.3 0 25 82 21 596.3 0 21 83 35 596.3 0 35 84 123 596.3 0 123 85 130 596.3 0 130 86 217 596.3 0 217 87 28 596.3 0 28 88 22 596.3 0 22 89 27 596.3 0 27 90 122 596.3 0 122 91 84 596.3 0 84 92 115 596.3 0 115 93 97 596.3 0 97 94 101 596.3 0 101 95 124 596.3 0 124 96 27 596.3 0 27 97 47 596.3 0 47 98 11 596.3 0 11 99 19 596.3 0 19 100 21 596.3 0 21 101 14 596.3 0 14 102 17 596.3 0 17 103 25 596.3 0 25 104 72 596.3 0 72 105 75 596.3 0 75 106 68 596.3 0 68 107 95 596.3 0 95 108 181 596.3 0 181 109 189 596.3 0 189 110 259 596.3 0 259 111 142 596.3 0 142 112 301 596.3 0 301 113 127 596.3 0 127 114 22 596.3 0 22 115 33 596.3 0 33 116 34 596.3 0 34 117 105 596.3 0 105 118 54 596.3 0 54 119 66 596.3 0 66 120 17 596.3 0 17 121 13 596.3 0 13 122 14 596.3 0 14 123 26 596.3 0 26 124 33 596.3 0 33 125 53 596.3 0 53 126 77 596.3 0 77 127 390 596.3 0 390 128 120 596.3 0 120 129 365 596.3 0 365 130 55 596.3 0 55 131 69 596.3 0 69 132 174 596.3 0 174 133 58 596.3 0 58 134 84 596.3 0 84 135 21 596.3 0 21 136 37 596.3 0 37 137 51 596.3 0 51 138 77 596.3 0 77 139 130 596.3 0 130 140 228 596.3 0 228 141 263 596.3 0 263 142 347 596.3 0 347 143 768 596.3 0 768 144 11387 596.3 0 11387 145 7622 596.3 0 7622 146 9034 596.3 0 9034 147 110 596.3 0 110 148 84 596.3 0 84 149 32 596.3 0 32 150 43 596.3 0 43 151 249 596.3 0 249 152 193 596.3 0 193 153 297 596.3 0 297 154 130 596.3 0 130 155 90 596.3 0 90 156 196 596.3 0 196 157 311 596.3 0 311 158 208 596.3 0 208 159 84 596.3 0 84 160 52 596.3 0 52 161 69 596.3 0 69 162 27 596.3 0 27 163 25 596.3 0 25 164 22 596.3 0 22 165 42 596.3 0 42 166 42 596.3 0 42 167 25 596.3 0 25 168 28 596.3 0 28 169 35 596.3 0 35 170 56 596.3 0 56 171 82 596.3 0 82 172 132 596.3 0 132 173 70 596.3 0 70 174 56 596.3 0 56 175 38 596.3 0 38 176 40 596.3 0 40 177 52 596.3 0 52 178 65 596.3 0 65 179 310 596.3 0 310 180 101 596.3 0 101 181 249 596.3 0 249 182 37 596.3 0 37 183 23 596.3 0 23 184 43 596.3 0 43 185 48 596.3 0 48 186 59 596.3 0 59 187 37 596.3 0 37 188 38 596.3 0 38 189 43 596.3 0 43 190 218 596.3 0 218 191 84 596.3 0 84 192 280 596.3 0 280 193 48 596.3 0 48 194 35 596.3 0 35 195 29 596.3 0 29 196 40 596.3 0 40 197 71 596.3 0 71 198 150 596.3 0 150 199 90 596.3 0 90 200 79 596.3 0 79 201 66 596.3 0 66 202 27 596.3 0 27 203 49 596.3 0 49 204 33 596.3 0 33 205 46 596.3 0 46 206 58 596.3 0 58 207 70 596.3 0 70 208 27 596.3 0 27 209 42 596.3 0 42 210 26 596.3 0 26 211 18 596.3 0 18 212 43 596.3 0 43 213 36 596.3 0 36 214 32 596.3 0 32 215 21 596.3 0 21 216 30 596.3 0 30 217 103 596.3 0 103 218 81 596.3 0 81 219 508 596.3 0 508 220 30 596.3 0 30 221 22 596.3 0 22 222 32 596.3 0 32 223 28 596.3 0 28 224 34 596.3 0 34 225 76 596.3 0 76 226 49 596.3 0 49 227 89 596.3 0 89 228 98 596.3 0 98 229 78 596.3 0 78 230 196 596.3 0 196 231 140 596.3 0 140 232 166 596.3 0 166 233 88 596.3 0 88 234 86 596.3 0 86 235 281 596.3 0 281 236 595 596.3 0 595 237 562 596.3 0 562 238 731 596.3 0 731 239 154 596.3 0 154 240 808 596.3 0 808 241 299 596.3 0 299 242 813 596.3 0 813 243 97 596.3 0 97 244 70 596.3 0 70 245 143 596.3 0 143 246 147 596.3 0 147 247 220 596.3 0 220 248 178 596.3 0 178 249 160 596.3 0 160 250 335 596.3 0 335 251 561 596.3 0 561 252 595 596.3 0 595 253 154 596.3 0 154 254 73 596.3 0 73 255 101 596.3 0 101 256 158 596.3 0 158 257 147 596.3 0 147 258 237 596.3 0 237 259 539 596.3 0 539 260 6680 596.3 0 6680 261 4013 596.3 0 4013 262 6203 596.3 0 6203 263 1532 596.3 0 1532 264 70 596.3 0 70 265 64 596.3 0 64 266 101 596.3 0 101 267 180 596.3 0 180 268 184 596.3 0 184 269 140 596.3 0 140 270 280 596.3 0 280 271 461 596.3 0 461 272 357 596.3 0 357 273 125 596.3 0 125 274 72 596.3 0 72 275 54 596.3 0 54 276 122 596.3 0 122 277 191 596.3 0 191 278 171 596.3 0 171 279 247 596.3 0 247 280 340 596.3 0 340 281 445 596.3 0 445 282 463 596.3 0 463 283 2086 596.3 0 2086 284 6386 596.3 0 6386 285 1421 596.3 0 1421 286 101 596.3 0 101 287 141 596.3 0 141 288 350 596.3 0 350 289 256 596.3 0 256 290 255 596.3 0 255 291 407 596.3 0 407 292 510 596.3 0 510 293 908 596.3 0 908 294 635 596.3 0 635 295 497 596.3 0 497 296 537 596.3 0 537 297 132 596.3 0 132 298 49 596.3 0 49 299 57 596.3 0 57 300 53 596.3 0 53 301 65 596.3 0 65 Finished in 2.46 s (40 us/read; 1.50 M reads/minute). === Summary === Total reads processed: 61,646 Reads with adapters: 9,891 (16.0%) Reads that were too short: 73 (0.1%) Reads written (passing filters): 9,818 (15.9%) Total basepairs processed: 12,483,161 bp Total written (filtered): 2,700,745 bp (21.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 9891 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 8 0.0 3 0 0 6 2 16 102 0.0 3 0 87 5 10 17 9577 0.0 3 8965 498 84 30 18 18 0.0 3 0 16 1 1 19 29 0.0 3 1 0 15 13 20 20 0.0 3 0 0 0 20 23 1 0.0 3 0 0 1 25 1 0.0 3 0 0 0 1 29 2 0.0 3 0 0 0 2 30 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 34 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 40 3 0.0 3 0 0 0 3 42 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 44 7 0.0 3 0 0 0 7 47 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 50 3 0.0 3 0 0 0 3 53 2 0.0 3 0 0 0 2 55 3 0.0 3 0 0 0 3 57 2 0.0 3 0 0 0 2 60 1 0.0 3 0 0 0 1 62 5 0.0 3 0 0 0 5 63 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 69 3 0.0 3 0 0 0 3 70 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 74 2 0.0 3 0 0 1 1 76 3 0.0 3 0 0 0 3 80 2 0.0 3 0 0 0 2 81 2 0.0 3 0 0 0 2 84 1 0.0 3 0 0 0 1 87 2 0.0 3 0 0 0 2 88 1 0.0 3 0 0 0 1 95 2 0.0 3 0 0 0 2 97 2 0.0 3 0 1 0 1 98 2 0.0 3 0 0 0 2 101 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 106 2 0.0 3 0 0 0 2 109 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 128 2 0.0 3 0 0 0 2 129 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 142 3 0.0 3 0 0 0 3 143 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 145 2 0.0 3 0 0 2 148 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 151 7 0.0 3 0 0 1 6 155 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 173 2 0.0 3 0 0 0 2 174 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 177 3 0.0 3 0 0 0 3 178 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 1 193 1 0.0 3 0 0 0 1 194 3 0.0 3 0 0 0 3 204 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 221 4 0.0 3 0 0 0 4 234 1 0.0 3 0 0 0 1 237 2 0.0 3 0 0 0 2 279 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.36 s (37 us/read; 1.64 M reads/minute). === Summary === Total reads processed: 9,818 Reads with adapters: 143 (1.5%) Reads written (passing filters): 9,818 (100.0%) Total basepairs processed: 2,700,745 bp Total written (filtered): 2,700,307 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 143 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 16.1% C: 30.8% G: 48.3% T: 4.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 138 153.4 0 138 4 1 38.4 0 1 5 4 9.6 0 4